BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= maV30281 (638 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC24C6.05 |sec28||coatomer epsilon subunit |Schizosaccharomyce... 45 8e-06 SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|S... 27 2.3 SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1 |Schizosa... 27 2.3 SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizos... 27 3.0 SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 26 5.3 SPAP27G11.02 |||TPR repeat protein, unknown biological role|Schi... 26 5.3 SPMIT.06 |||mitochondrial DNA binding endonuclease|Schizosacchar... 25 7.0 SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomy... 25 7.0 SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 25 9.2 >SPBC24C6.05 |sec28||coatomer epsilon subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 288 Score = 45.2 bits (102), Expect = 8e-06 Identities = 48/194 (24%), Positives = 86/194 (44%), Gaps = 2/194 (1%) Frame = +3 Query: 18 DELFDVKNAFYVGNYQQAINEAQSVSPSTPLVALQRDAFLYRSYIAQGNYRIVQQELKTA 197 +EL+ V+ FY GNY + E + S S + L + ++ R+ +A G Q + T Sbjct: 10 NELYFVRQYFYSGNYTKLF-EIDTTSMSEKGLELT-EIYMARAKLALGESLESIQSILTQ 67 Query: 198 DPMLQPLKSLVDYLLPDANKSAIVADIDARVAKGTELSNEIFLIVAATIYYHEDNYEAAL 377 P + + L + N I+ G S+ + + A +++ A+ Sbjct: 68 KT---PGSAAILALAGEGNMELIIDQ------HGN--SDSVVQTLGAIFQIKNGSFDDAM 116 Query: 378 KILHNA-ESLELRAFTLQCLLAMNRPDLARKQLKLLQDIEDDGTLTQLAQAWLNLIQGG- 551 +L + E+LE A + L ++ + A + LK D D+ + QLAQ+W+ ++ GG Sbjct: 117 DLLKKSVENLEAVALQVYIHLREHKIEAAEQTLKQALDWADEEIVLQLAQSWIKIVSGGV 176 Query: 552 PGIQDAHYSVMELS 593 DA Y EL+ Sbjct: 177 ESYNDAFYVFEELN 190 >SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|Schizosaccharomyces pombe|chr 2|||Manual Length = 2337 Score = 27.1 bits (57), Expect = 2.3 Identities = 10/31 (32%), Positives = 20/31 (64%) Frame = +1 Query: 298 VRSCPMKYS*LWLQQFTIMKIIMKLH*KSFI 390 +R+CP + QQ +I+ +I++ H +SF+ Sbjct: 857 MRTCPTNILEFYFQQLSILVLIVRQHIRSFL 887 >SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 642 Score = 27.1 bits (57), Expect = 2.3 Identities = 18/50 (36%), Positives = 26/50 (52%), Gaps = 3/50 (6%) Frame = +3 Query: 369 AALKILHNAESLELRAFTLQCLL---AMNRPDLARKQLKLLQDIEDDGTL 509 A +K+ H+ + L T+ L AM DL +K +LLQ I DDG + Sbjct: 267 AQVKLYHSIKPLFANKVTILVLNKIDAMRPEDLDQKNQELLQTIIDDGNV 316 >SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizosaccharomyces pombe|chr 2|||Manual Length = 872 Score = 26.6 bits (56), Expect = 3.0 Identities = 23/97 (23%), Positives = 44/97 (45%), Gaps = 2/97 (2%) Frame = +3 Query: 60 YQQAINEAQSVSPSTPLVALQRD-AFLYRSYI-AQGNYRIVQQELKTADPMLQPLKSLVD 233 Y + ++ S +T L LYR+ +G+Y IVQ+ ++A+P+ + Sbjct: 635 YSPSHKDSNKTSRNTSKEGLPSSPTMLYRTTSNTRGDY-IVQRTQQSANPLTNIEPQDMS 693 Query: 234 YLLPDANKSAIVADIDARVAKGTELSNEIFLIVAATI 344 L D N S +VAD + T ++ + +A+ + Sbjct: 694 NLSTDINASDVVADSTNSILYPTSTASSVANTIASDV 730 >SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces pombe|chr 1|||Manual Length = 670 Score = 25.8 bits (54), Expect = 5.3 Identities = 19/59 (32%), Positives = 32/59 (54%) Frame = +3 Query: 342 IYYHEDNYEAALKILHNAESLELRAFTLQCLLAMNRPDLARKQLKLLQDIEDDGTLTQL 518 ++++ Y+ L + N S++ T + LL N L+ K LKL ++I+D TL QL Sbjct: 381 VHWNSTVYQELLNLKSNNSSVD-GVKTRRQLLEENAL-LSHKVLKLTEEIQDLETLNQL 437 >SPAP27G11.02 |||TPR repeat protein, unknown biological role|Schizosaccharomyces pombe|chr 1|||Manual Length = 356 Score = 25.8 bits (54), Expect = 5.3 Identities = 26/102 (25%), Positives = 46/102 (45%), Gaps = 4/102 (3%) Frame = +3 Query: 237 LLPDANKSA--IVADIDARVAKGTELSNEIFLIVAATIYYHEDNYEAALKILHNAESLEL 410 L DA K+A IV + ++ G + +AT+Y+ A+ + H A +L + Sbjct: 166 LQKDATKAADLIVKALLSKQFNGDDEQKSRLFEQSATLYFQAGTPSYAVPLYHEALNLTM 225 Query: 411 RAFTLQCLLAMNRPDLARKQLKLLQ--DIEDDGTLTQLAQAW 530 + L+ MN +LA L + D + TL + +Q+W Sbjct: 226 ANPSCHGLILMN--NLATSLLAQTETVDKKHHETLMKQSQSW 265 >SPMIT.06 |||mitochondrial DNA binding endonuclease|Schizosaccharomyces pombe|chr mitochondrial|||Manual Length = 807 Score = 25.4 bits (53), Expect = 7.0 Identities = 11/37 (29%), Positives = 20/37 (54%) Frame = +3 Query: 156 QGNYRIVQQELKTADPMLQPLKSLVDYLLPDANKSAI 266 Q Y++ ++ L+P+K +DYL+ AN+ I Sbjct: 749 QSTYKVEMHHVRQMKN-LKPIKGTLDYLMAKANRKQI 784 >SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 537 Score = 25.4 bits (53), Expect = 7.0 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = +3 Query: 324 LIVAATIYYHEDNYEAALKILHNAESLELRAFTLQCL 434 LI+ Y H Y + ++ILHN E +L F +CL Sbjct: 214 LIMKQFSYSHHMGYISEIRILHN-EYEKLLKFVRECL 249 >SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual Length = 4717 Score = 25.0 bits (52), Expect = 9.2 Identities = 9/27 (33%), Positives = 18/27 (66%) Frame = +3 Query: 117 LQRDAFLYRSYIAQGNYRIVQQELKTA 197 ++R AFLY ++ ++ ++ QEL+ A Sbjct: 346 IKRVAFLYHKFMIPSDHVVISQELQDA 372 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,589,671 Number of Sequences: 5004 Number of extensions: 52255 Number of successful extensions: 130 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 125 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 129 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 285732116 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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