Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= maV30280
         (627 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase |Schizosaccharom...   135   4e-33
SPBC902.05c |idh2|glu2|isocitrate dehydrogenase |Schizosaccharom...    91   1e-19
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ...    44   2e-05
SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces pombe...    33   0.045
SPAC29B12.14c |||purine transporter |Schizosaccharomyces pombe|c...    27   2.2  
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo...    26   3.9  
SPBC14F5.12c |cbh2||centromere binding protein Cbh2|Schizosaccha...    26   5.1  
SPAC17G6.04c |cpp1||protein farnesyltransferase beta subunit Cpp...    25   6.8  
SPAC15A10.03c |rhp54|rad54|Rad54 homolog Rhp54|Schizosaccharomyc...    25   6.8  
SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated prote...    25   9.0  
SPAC1687.16c |erg3||C-5 sterol desaturase Erg3 |Schizosaccharomy...    25   9.0  
SPAC22E12.03c |||THIJ/PFPI family peptidase |Schizosaccharomyces...    25   9.0  

>SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 356

 Score =  135 bits (327), Expect = 4e-33
 Identities = 71/154 (46%), Positives = 96/154 (62%), Gaps = 4/154 (2%)
 Frame = +3

Query: 150 VIRKQKLIPKAQYGGRHAVTMLPGGGIGPECMGYVRDIFKYIGAPIDFEVVDIDPTMDND 329
           ++RK       +YGG++ VT++PG GIG E    V +IFK    PI+FE +D+     N+
Sbjct: 5   LVRKSSAFQPLKYGGKYTVTLIPGDGIGRETSNAVTEIFKTANVPIEFEEIDVTGMEKNN 64

Query: 330 ----DDVQYAITTIKRNGVGLKGNIETKSEAAYVTSRNVALRNELDMYAYILNCKSYPGV 497
               D +  AI ++KRN VGLKG + T  E    TS NVALR ELD+YA ++  K+ PG 
Sbjct: 65  KSSGDALHEAIQSLKRNKVGLKGILFTPFEKGGHTSFNVALRKELDIYASLVLIKNIPGF 124

Query: 498 ATRHKDIDVVIIRQNTEGEYAMLEHESVEWVWSS 599
            TRH ++D  IIR+NTEGEY+ LEH+SV  V  S
Sbjct: 125 KTRHDNVDFAIIRENTEGEYSGLEHQSVPGVVES 158


>SPBC902.05c |idh2|glu2|isocitrate dehydrogenase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 378

 Score = 91.1 bits (216), Expect = 1e-19
 Identities = 51/138 (36%), Positives = 76/138 (55%), Gaps = 2/138 (1%)
 Frame = +3

Query: 192 GRHAVTMLPGGGIGPECMGYVRDIFKYIGAPIDFEVVDIDPTMDNDDDV--QYAITTIKR 365
           G + VTM+ G GIGPE    V  IFK    PI++E V + P + N        A  ++++
Sbjct: 44  GNYTVTMIAGDGIGPEIAQSVERIFKAAKVPIEWERVKVYPILKNGTTTIPDDAKESVRK 103

Query: 366 NGVGLKGNIETKSEAAYVTSRNVALRNELDMYAYILNCKSYPGVATRHKDIDVVIIRQNT 545
           N V LKG + T     +V S N+ LR    ++A +  C S  G  T + +++ V+IR+NT
Sbjct: 104 NKVALKGPLATPIGKGHV-SMNLTLRRTFGLFANVRPCVSITGYKTPYDNVNTVLIRENT 162

Query: 546 EGEYAMLEHESVEWVWSS 599
           EGEY+ +EHE +  V  S
Sbjct: 163 EGEYSGIEHEVIPGVVQS 180


>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 362

 Score = 44.0 bits (99), Expect = 2e-05
 Identities = 37/134 (27%), Positives = 67/134 (50%), Gaps = 9/134 (6%)
 Frame = +3

Query: 195 RHAVTMLPGGGIGPECMGYVRDIFKYIGAP--IDFEVVDIDPTMDNDDDVQYAIT--TIK 362
           R  + ++P  GIG E +   R + + + A   + F+ +D+D      +    A+   T++
Sbjct: 6   RIVLGLIPADGIGKEVVPAARRLMENLPAKHKLKFDFIDLDAGWGTFERTGKALPERTVE 65

Query: 363 RN----GVGLKGNIETKS-EAAYVTSRNVALRNELDMYAYILNCKSYPGVATRHKDIDVV 527
           R        L G +++ + + A  +S  VALR ++ +YA +   KS  G   + K +D+V
Sbjct: 66  RLKTECNAALFGAVQSPTHKVAGYSSPIVALRKKMGLYANVRPVKSLDGA--KGKPVDLV 123

Query: 528 IIRQNTEGEYAMLE 569
           I+R+NTE  Y   E
Sbjct: 124 IVRENTECLYVKEE 137


>SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 734

 Score = 32.7 bits (71), Expect = 0.045
 Identities = 16/51 (31%), Positives = 27/51 (52%)
 Frame = +3

Query: 318 MDNDDDVQYAITTIKRNGVGLKGNIETKSEAAYVTSRNVALRNELDMYAYI 470
           M +DD VQY I  +K  G+ L  +++T  E   +  +    R + D+Y Y+
Sbjct: 685 MKHDDLVQYVINNVKDRGIPLVSDVKTAIEK--LLEKEYLEREDNDIYTYV 733


>SPAC29B12.14c |||purine transporter |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 581

 Score = 27.1 bits (57), Expect = 2.2
 Identities = 17/54 (31%), Positives = 26/54 (48%), Gaps = 2/54 (3%)
 Frame = +3

Query: 366 NGVGLKGNIETKSEAAYVTSRNVA--LRNELDMYAYILNCKSYPGVATRHKDID 521
           N VGL G +  K   A +T  N+A  L       ++++ CK +P  A   K +D
Sbjct: 470 NVVGLAGAVGDKVPKAALTMNNIAYLLGIVTSFLSHLIICKIFPVTACGEKFLD 523


>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1224

 Score = 26.2 bits (55), Expect = 3.9
 Identities = 15/46 (32%), Positives = 23/46 (50%)
 Frame = -3

Query: 337 TSSSLSIVGSMSTTSKSIGAPMYLNMSRTYPMHSGPIPPPGSMVTA 200
           TS + SI+ ++  T+ S  +P     S   P    P+PPP +  TA
Sbjct: 816 TSQASSILPTVPRTT-SYTSPYATTSSHITPADVHPLPPPSTSTTA 860


>SPBC14F5.12c |cbh2||centromere binding protein
           Cbh2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 514

 Score = 25.8 bits (54), Expect = 5.1
 Identities = 8/19 (42%), Positives = 15/19 (78%)
 Frame = +3

Query: 531 IRQNTEGEYAMLEHESVEW 587
           IR+N +G+Y +LE+  ++W
Sbjct: 71  IRRNRQGKYPILENALIDW 89


>SPAC17G6.04c |cpp1||protein farnesyltransferase beta subunit
           Cpp1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 382

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 13/39 (33%), Positives = 22/39 (56%), Gaps = 4/39 (10%)
 Frame = -3

Query: 400 LVSMLPFSPTPFLLMVV----IAYWTSSSLSIVGSMSTT 296
           L  ML   P+PF ++      + YW  SSL+I+G + ++
Sbjct: 38  LTKMLDPLPSPFTVLDASRAWMVYWELSSLAILGKLDSS 76


>SPAC15A10.03c |rhp54|rad54|Rad54 homolog Rhp54|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 852

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 10/34 (29%), Positives = 20/34 (58%)
 Frame = -2

Query: 362 LNGRYSILDIIIIVHRWVNVHHFEVNRSTDVFEY 261
           L+G+ S  ++I+ + +W +VH  +V R   +  Y
Sbjct: 356 LDGKSSKQELIMALQQWASVHGRQVTRPVLIASY 389


>SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated protein
           Mug36|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1646

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 11/26 (42%), Positives = 16/26 (61%)
 Frame = -2

Query: 587 PFNGFVFQHSIFTFCVLSDNNNINVL 510
           P NG VF+  I    + +DNN+I+ L
Sbjct: 416 PSNGAVFEMKINQIQIFNDNNDIDEL 441


>SPAC1687.16c |erg3||C-5 sterol desaturase Erg3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 300

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 9/20 (45%), Positives = 10/20 (50%)
 Frame = -1

Query: 429 YVMSHRLPHFWFQCCPLAPH 370
           Y   H+L H W  C P A H
Sbjct: 146 YPRLHKLHHKWIICTPYASH 165


>SPAC22E12.03c |||THIJ/PFPI family peptidase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 191

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 10/31 (32%), Positives = 19/31 (61%), Gaps = 1/31 (3%)
 Frame = +3

Query: 156 RKQKLIPKAQ-YGGRHAVTMLPGGGIGPECM 245
           R  K IP A  +  ++ + ++PGGG+G + +
Sbjct: 56  RSYKEIPSADDFAKQYDIAIIPGGGLGAKTL 86


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,717,254
Number of Sequences: 5004
Number of extensions: 57754
Number of successful extensions: 182
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 179
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 277683324
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)