Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30274
(680 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g30970.1 68415.m03777 aspartate aminotransferase, mitochondri... 170 8e-43
At4g31990.2 68417.m04554 aspartate aminotransferase, chloroplast... 168 3e-42
At4g31990.1 68417.m04553 aspartate aminotransferase, chloroplast... 168 3e-42
At5g11520.1 68418.m01344 aspartate aminotransferase, chloroplast... 163 1e-40
At5g19550.1 68418.m02328 aspartate aminotransferase, cytoplasmic... 162 2e-40
At1g62800.1 68414.m07089 aspartate aminotransferase, cytoplasmic... 142 3e-34
At1g62800.2 68414.m07090 aspartate aminotransferase, cytoplasmic... 139 2e-33
At4g37235.1 68417.m05271 expressed protein 30 1.2
At3g54460.1 68416.m06025 SNF2 domain-containing protein / helica... 29 2.8
At3g54000.3 68416.m05969 expressed protein 28 5.0
At3g54000.2 68416.m05968 expressed protein 28 5.0
At3g54000.1 68416.m05970 expressed protein 28 5.0
At1g75530.1 68414.m08778 forkhead-associated domain-containing p... 28 6.6
At3g11325.1 68416.m01377 hypothetical protein 27 8.7
>At2g30970.1 68415.m03777 aspartate aminotransferase, mitochondrial
/ transaminase A (ASP1) identical to SP|P46643 Aspartate
aminotransferase, mitochondrial precursor (EC 2.6.1.1)
(Transaminase A) {Arabidopsis thaliana}
Length = 430
Score = 170 bits (413), Expect = 8e-43
Identities = 84/184 (45%), Positives = 113/184 (61%)
Frame = +2
Query: 59 GLRASSTWWNNVQMGPPDVILGITEAYKKDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAE 238
GLR+ S+WW +V+ P D ILG+TEA+ D P+KVN+GVGAYRDD GKP VL VR+AE
Sbjct: 25 GLRSMSSWWKSVEPAPKDPILGVTEAFLADPSPEKVNVGVGAYRDDNGKPVVLECVREAE 84
Query: 239 EILHSRGLNHEYAPISGEATYTDAVAKLAFGEDSPVIKNKSNCTVQTLSGTGALRLGLEF 418
+ L + EY P+ G A D KLA+G++S IK+K VQTLSGTGA RL +F
Sbjct: 85 KRL-AGSTFMEYLPMGGSAKMVDLTLKLAYGDNSEFIKDKRIAAVQTLSGTGACRLFADF 143
Query: 419 ITKHYAKAKEIWLPTPTWGNHPQICNTLNLPHKKYRYFDPKTNGFDLQGSTRGYIKNSRR 598
K ++ +I++P PTW NH I +P K Y Y+ P+T G D + +KN+
Sbjct: 144 -QKRFSPGSQIYIPVPTWSNHHNIWKDAQVPQKTYHYYHPETKGLDF-SALMDDVKNAPE 201
Query: 599 FHYF 610
+F
Sbjct: 202 GSFF 205
Score = 48.0 bits (109), Expect = 6e-06
Identities = 18/29 (62%), Positives = 21/29 (72%)
Frame = +1
Query: 592 PKVPLFLLHACAHNPTGVDPKPNDWEQLS 678
P+ FLLHACAHNPTGVDP W ++S
Sbjct: 200 PEGSFFLLHACAHNPTGVDPTEEQWREIS 228
>At4g31990.2 68417.m04554 aspartate aminotransferase, chloroplast /
transaminase A (ASP5) (AAT1) nearly identical to
SP|P46248 Aspartate aminotransferase, chloroplast
precursor (EC 2.6.1.1) (Transaminase A) {Arabidopsis
thaliana}
Length = 453
Score = 168 bits (409), Expect = 3e-42
Identities = 79/161 (49%), Positives = 107/161 (66%)
Frame = +2
Query: 83 WNNVQMGPPDVILGITEAYKKDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAEEILHSRGL 262
+ + M PPD ILG++EA+K DT+ K+NLGVGAYR +E +P+VL V+KAE ++ RG
Sbjct: 53 FEGITMAPPDPILGVSEAFKADTNGMKLNLGVGAYRTEELQPYVLNVVKKAENLMLERGD 112
Query: 263 NHEYAPISGEATYTDAVAKLAFGEDSPVIKNKSNCTVQTLSGTGALRLGLEFITKHYAKA 442
N EY PI G A + A A+L FG PVIK + T+Q LSGTG+LRL I +++ A
Sbjct: 113 NKEYLPIEGLAAFNKATAELLFGAGHPVIKEQRVATIQGLSGTGSLRLAAALIERYFPGA 172
Query: 443 KEIWLPTPTWGNHPQICNTLNLPHKKYRYFDPKTNGFDLQG 565
K + + +PTWGNH I N +P +YRY+DPKT G D +G
Sbjct: 173 KVV-ISSPTWGNHKNIFNDAKVPWSEYRYYDPKTIGLDFEG 212
Score = 44.4 bits (100), Expect = 7e-05
Identities = 15/29 (51%), Positives = 20/29 (68%)
Frame = +1
Query: 592 PKVPLFLLHACAHNPTGVDPKPNDWEQLS 678
P+ LLH CAHNPTG+DP P W +++
Sbjct: 221 PEGSFILLHGCAHNPTGIDPTPEQWVKIA 249
>At4g31990.1 68417.m04553 aspartate aminotransferase, chloroplast /
transaminase A (ASP5) (AAT1) nearly identical to
SP|P46248 Aspartate aminotransferase, chloroplast
precursor (EC 2.6.1.1) (Transaminase A) {Arabidopsis
thaliana}
Length = 453
Score = 168 bits (409), Expect = 3e-42
Identities = 79/161 (49%), Positives = 107/161 (66%)
Frame = +2
Query: 83 WNNVQMGPPDVILGITEAYKKDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAEEILHSRGL 262
+ + M PPD ILG++EA+K DT+ K+NLGVGAYR +E +P+VL V+KAE ++ RG
Sbjct: 53 FEGITMAPPDPILGVSEAFKADTNGMKLNLGVGAYRTEELQPYVLNVVKKAENLMLERGD 112
Query: 263 NHEYAPISGEATYTDAVAKLAFGEDSPVIKNKSNCTVQTLSGTGALRLGLEFITKHYAKA 442
N EY PI G A + A A+L FG PVIK + T+Q LSGTG+LRL I +++ A
Sbjct: 113 NKEYLPIEGLAAFNKATAELLFGAGHPVIKEQRVATIQGLSGTGSLRLAAALIERYFPGA 172
Query: 443 KEIWLPTPTWGNHPQICNTLNLPHKKYRYFDPKTNGFDLQG 565
K + + +PTWGNH I N +P +YRY+DPKT G D +G
Sbjct: 173 KVV-ISSPTWGNHKNIFNDAKVPWSEYRYYDPKTIGLDFEG 212
Score = 44.4 bits (100), Expect = 7e-05
Identities = 15/29 (51%), Positives = 20/29 (68%)
Frame = +1
Query: 592 PKVPLFLLHACAHNPTGVDPKPNDWEQLS 678
P+ LLH CAHNPTG+DP P W +++
Sbjct: 221 PEGSFILLHGCAHNPTGIDPTPEQWVKIA 249
>At5g11520.1 68418.m01344 aspartate aminotransferase, chloroplast /
transaminase A (ASP3) (YLS4) identical to SP|P46644
Aspartate aminotransferase, chloroplast precursor (EC
2.6.1.1) (Transaminase A) {Arabidopsis thaliana};
identical to cDNA YLS4 mRNA for aspartate
aminotransferase (ASP3), partial cds GI:13122285
Length = 449
Score = 163 bits (396), Expect = 1e-40
Identities = 81/162 (50%), Positives = 104/162 (64%), Gaps = 1/162 (0%)
Frame = +2
Query: 83 WNNVQMGPPDVILGITEAYKKDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAE-EILHSRG 259
++++ P D ILG+T AY KD P K+NLGVGAYR +EGKP VL VRKAE ++++ R
Sbjct: 49 FSHLVQAPEDPILGVTVAYNKDPSPVKLNLGVGAYRTEEGKPLVLNVVRKAEQQLINDRT 108
Query: 260 LNHEYAPISGEATYTDAVAKLAFGEDSPVIKNKSNCTVQTLSGTGALRLGLEFITKHYAK 439
EY PI G + AKL G DSP I+ TV+ LSGTG+LR+G EF+ KHY +
Sbjct: 109 RIKEYLPIVGLVEFNKLSAKLILGADSPAIRENRITTVECLSGTGSLRVGGEFLAKHYHQ 168
Query: 440 AKEIWLPTPTWGNHPQICNTLNLPHKKYRYFDPKTNGFDLQG 565
K I++ PTWGNHP+I L K YRY+DP T G + QG
Sbjct: 169 -KTIYITQPTWGNHPKIFTLAGLTVKTYRYYDPATRGLNFQG 209
Score = 45.2 bits (102), Expect = 4e-05
Identities = 17/24 (70%), Positives = 19/24 (79%)
Frame = +1
Query: 604 LFLLHACAHNPTGVDPKPNDWEQL 675
+ LLHACAHNPTGVDP WEQ+
Sbjct: 222 IVLLHACAHNPTGVDPTIQQWEQI 245
>At5g19550.1 68418.m02328 aspartate aminotransferase, cytoplasmic
isozyme 1 / transaminase A (ASP2) identical to SP|P46645
Aspartate aminotransferase, cytoplasmic isozyme 1 (EC
2.6.1.1) (Transaminase A) {Arabidopsis thaliana}
Length = 405
Score = 162 bits (393), Expect = 2e-40
Identities = 79/162 (48%), Positives = 103/162 (63%), Gaps = 1/162 (0%)
Frame = +2
Query: 83 WNNVQMGPPDVILGITEAYKKDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAE-EILHSRG 259
++NV P D ILG+T AY D P K+NLGVGAYR +EGKP VL VRKAE ++++
Sbjct: 5 FSNVARAPEDPILGVTVAYNNDPSPVKINLGVGAYRTEEGKPLVLDVVRKAEQQLVNDPS 64
Query: 260 LNHEYAPISGEATYTDAVAKLAFGEDSPVIKNKSNCTVQTLSGTGALRLGLEFITKHYAK 439
EY PI G + + AKL G DSP I TVQ LSGTG+LR+G EF+ HY +
Sbjct: 65 RVKEYIPIVGISDFNKLSAKLILGADSPAITESRVTTVQCLSGTGSLRVGAEFLKTHYHQ 124
Query: 440 AKEIWLPTPTWGNHPQICNTLNLPHKKYRYFDPKTNGFDLQG 565
+ I++P PTWGNHP++ N L + +RY+DP T G D +G
Sbjct: 125 S-VIYIPKPTWGNHPKVFNLAGLSVEYFRYYDPATRGLDFKG 165
Score = 47.2 bits (107), Expect = 1e-05
Identities = 18/28 (64%), Positives = 20/28 (71%)
Frame = +1
Query: 592 PKVPLFLLHACAHNPTGVDPKPNDWEQL 675
P + LLHACAHNPTGVDP WEQ+
Sbjct: 174 PSGAIVLLHACAHNPTGVDPTSEQWEQI 201
>At1g62800.1 68414.m07089 aspartate aminotransferase, cytoplasmic
isozyme 2 / transaminase A (ASP4) identical to aspartate
aminotransferase, cytoplasmic isozyme 2 SP:P46646
[Arabidopsis thaliana]
Length = 403
Score = 142 bits (343), Expect = 3e-34
Identities = 69/160 (43%), Positives = 96/160 (60%)
Frame = +2
Query: 86 NNVQMGPPDVILGITEAYKKDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAEEILHSRGLN 265
++V P D +L + A + D P K+NL G YR +EGKP VL VR+AE+ L + L+
Sbjct: 6 SSVLPAPEDPVLSVIFACRDDPSPVKLNLSAGTYRTEEGKPLVLDVVRRAEQQL-ANDLD 64
Query: 266 HEYAPISGEATYTDAVAKLAFGEDSPVIKNKSNCTVQTLSGTGALRLGLEFITKHYAKAK 445
EY P++G + KL G+DSP +K T Q LSGTG+LR+G EF+ H K
Sbjct: 65 KEYLPLNGLPEFNKLSTKLILGDDSPALKENRVVTTQCLSGTGSLRVGAEFLATH-NKES 123
Query: 446 EIWLPTPTWGNHPQICNTLNLPHKKYRYFDPKTNGFDLQG 565
I++P PTWGNHP+I L + +RY+DPK+ G D +G
Sbjct: 124 VIFVPNPTWGNHPRIFTLAGLSVQYFRYYDPKSRGLDFKG 163
Score = 39.5 bits (88), Expect = 0.002
Identities = 15/28 (53%), Positives = 19/28 (67%)
Frame = +1
Query: 592 PKVPLFLLHACAHNPTGVDPKPNDWEQL 675
P + +L ACAHNPTGVDP WE++
Sbjct: 172 PPGAIVVLQACAHNPTGVDPTFEQWEKI 199
>At1g62800.2 68414.m07090 aspartate aminotransferase, cytoplasmic
isozyme 2 / transaminase A (ASP4) identical to aspartate
aminotransferase, cytoplasmic isozyme 2 SP:P46646
[Arabidopsis thaliana]
Length = 405
Score = 139 bits (336), Expect = 2e-33
Identities = 68/161 (42%), Positives = 95/161 (59%), Gaps = 1/161 (0%)
Frame = +2
Query: 86 NNVQMGPPDVILGITEAYKKDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAEEIL-HSRGL 262
++V P D +L + A + D P K+NL G YR +EGKP VL VR+AE+ L +
Sbjct: 6 SSVLPAPEDPVLSVIFACRDDPSPVKLNLSAGTYRTEEGKPLVLDVVRRAEQQLANDLSR 65
Query: 263 NHEYAPISGEATYTDAVAKLAFGEDSPVIKNKSNCTVQTLSGTGALRLGLEFITKHYAKA 442
+ EY P++G + KL G+DSP +K T Q LSGTG+LR+G EF+ H K
Sbjct: 66 DKEYLPLNGLPEFNKLSTKLILGDDSPALKENRVVTTQCLSGTGSLRVGAEFLATH-NKE 124
Query: 443 KEIWLPTPTWGNHPQICNTLNLPHKKYRYFDPKTNGFDLQG 565
I++P PTWGNHP+I L + +RY+DPK+ G D +G
Sbjct: 125 SVIFVPNPTWGNHPRIFTLAGLSVQYFRYYDPKSRGLDFKG 165
Score = 39.5 bits (88), Expect = 0.002
Identities = 15/28 (53%), Positives = 19/28 (67%)
Frame = +1
Query: 592 PKVPLFLLHACAHNPTGVDPKPNDWEQL 675
P + +L ACAHNPTGVDP WE++
Sbjct: 174 PPGAIVVLQACAHNPTGVDPTFEQWEKI 201
>At4g37235.1 68417.m05271 expressed protein
Length = 152
Score = 30.3 bits (65), Expect = 1.2
Identities = 14/44 (31%), Positives = 26/44 (59%), Gaps = 4/44 (9%)
Frame = -2
Query: 385 GQCLYSTVALIF----DDRAVFSKSQFSYSISICGLTTDGSILM 266
GQ L+S+ +L+F DD ++ + F Y +++ GL T S+ +
Sbjct: 19 GQTLFSSASLLFMCFNDDEDFYAYTTFCYLVTVMGLVTPWSVTL 62
>At3g54460.1 68416.m06025 SNF2 domain-containing protein / helicase
domain-containing protein / F-box family protein similar
to SP|P36607 DNA repair protein rad8 {Schizosaccharomyces
pombe}; contains Pfam profiles PF00271: Helicase
conserved C-terminal domain, PF00176: SNF2 family
N-terminal domain, PF00646: F-box domain
Length = 1378
Score = 29.1 bits (62), Expect = 2.8
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +2
Query: 344 VIKNKSNCTVQTLSGTGALRLGLEFITKHYAKAKEIW 454
+ +N ++C + G+GAL L L F+T H + IW
Sbjct: 1238 MFQNDADCMALLMDGSGALGLDLSFVT-HVFLMEPIW 1273
>At3g54000.3 68416.m05969 expressed protein
Length = 301
Score = 28.3 bits (60), Expect = 5.0
Identities = 9/24 (37%), Positives = 13/24 (54%)
Frame = -1
Query: 563 PANRNHWSWDRSNGISCAAS*GCC 492
PA +H +W+ + C A GCC
Sbjct: 97 PAGNDHKAWEMNRSPPCVAGTGCC 120
>At3g54000.2 68416.m05968 expressed protein
Length = 301
Score = 28.3 bits (60), Expect = 5.0
Identities = 9/24 (37%), Positives = 13/24 (54%)
Frame = -1
Query: 563 PANRNHWSWDRSNGISCAAS*GCC 492
PA +H +W+ + C A GCC
Sbjct: 97 PAGNDHKAWEMNRSPPCVAGTGCC 120
>At3g54000.1 68416.m05970 expressed protein
Length = 352
Score = 28.3 bits (60), Expect = 5.0
Identities = 9/24 (37%), Positives = 13/24 (54%)
Frame = -1
Query: 563 PANRNHWSWDRSNGISCAAS*GCC 492
PA +H +W+ + C A GCC
Sbjct: 97 PAGNDHKAWEMNRSPPCVAGTGCC 120
>At1g75530.1 68414.m08778 forkhead-associated domain-containing
protein / FHA domain-containing protein
Length = 555
Score = 27.9 bits (59), Expect = 6.6
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +2
Query: 446 EIWLPTPTWGNHPQICN 496
EIW+P P + HP+I N
Sbjct: 231 EIWIPPPPYDPHPEIVN 247
>At3g11325.1 68416.m01377 hypothetical protein
Length = 401
Score = 27.5 bits (58), Expect = 8.7
Identities = 16/47 (34%), Positives = 23/47 (48%), Gaps = 2/47 (4%)
Frame = +3
Query: 501 STCRTRNTVTSIPRPMVSI--CRGALEDISKIPEGSIISVARVRTQP 635
S+C+ R VT +PR MV I R LE I ++ ++ QP
Sbjct: 93 SSCKKRVVVTGMPRVMVEIRSPRSILEPIMSSIHALVMHSRKIELQP 139
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,079,594
Number of Sequences: 28952
Number of extensions: 404398
Number of successful extensions: 1050
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1005
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1038
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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