BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30268
(750 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC7D4.10 |vma13||V-type ATPase subunit H|Schizosaccharomyces p... 33 0.057
SPCC1450.16c |||triacylglycerol lipase|Schizosaccharomyces pombe... 28 1.2
SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit ... 28 1.6
SPCC16C4.02c |||DUF1941 family protein|Schizosaccharomyces pombe... 27 3.8
SPAC22F8.05 |||alpha,alpha-trehalose-phosphate synthase |Schizos... 26 5.0
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 26 5.0
SPAC23C11.16 |plo1||Polo kinase Plo1|Schizosaccharomyces pombe|c... 26 5.0
SPAC869.05c |||sulfate transporter |Schizosaccharomyces pombe|ch... 26 6.6
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 26 6.6
SPAC13C5.02 |dre4||DNA replication protein Dre4|Schizosaccharomy... 25 8.7
>SPAC7D4.10 |vma13||V-type ATPase subunit H|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 450
Score = 32.7 bits (71), Expect = 0.057
Identities = 34/173 (19%), Positives = 73/173 (42%), Gaps = 3/173 (1%)
Frame = +2
Query: 209 SEIRQTQINWQSYLQSQMITQRDHDFIVNLDQR---GQKDLPDKNPDACAEVFLNLLTHI 379
+ +R I WQ Y +S + + + I NL + +++ A + +FL LL+
Sbjct: 29 NNVRCVAIPWQGYQRSGSLEENELQEIENLTGKPLSAYVKTAEEDTTAYSNLFLKLLSMK 88
Query: 380 SKDHTIQYILVLIDDILSEDKSRVKIFRETKFSGNVWQPFLNLLNRQDEFVQHMTARIIA 559
+ + LV + D L + F + + + + +N D+ + + AR+ A
Sbjct: 89 DTPDVVNFALVKLADTLLNSNKFLSAFGPAFY--DFLEKDESYINYLDDDSKLLFARVFA 146
Query: 560 KLACWHPQLMDKSDLHFYLSWLKDQLKTNNNDYIQSVARCLQMMLRIDEYRFA 718
+ P + K+ +L +L +++ N +CL +L + +R+A
Sbjct: 147 LCSSSSPCSVAKA-FTLFLEYLGKLMQSLNPLTRLFAVQCLNGVLTLKAHRYA 198
>SPCC1450.16c |||triacylglycerol lipase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 513
Score = 28.3 bits (60), Expect = 1.2
Identities = 9/34 (26%), Positives = 22/34 (64%)
Frame = -3
Query: 706 FIDTQHHLQTPGHRLDIVVVIGLELIFKPGEIEV 605
F D HH + G+ L ++ ++GLE+ ++ ++++
Sbjct: 397 FYDELHHHRVSGYSLKMIRLVGLEMAYRFRQLDI 430
>SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit
Sec63 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 611
Score = 27.9 bits (59), Expect = 1.6
Identities = 14/36 (38%), Positives = 22/36 (61%), Gaps = 1/36 (2%)
Frame = -2
Query: 224 FGVSHLPGSVGRWWPQSCRFFR-HLALELTD*HFPR 120
FG+ LP +VG+WW S + R H+ ++ D FP+
Sbjct: 203 FGIV-LPYAVGKWWYGSRTYTRDHVHVDTVDEWFPK 237
>SPCC16C4.02c |||DUF1941 family protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 548
Score = 26.6 bits (56), Expect = 3.8
Identities = 16/66 (24%), Positives = 35/66 (53%)
Frame = +2
Query: 464 ETKFSGNVWQPFLNLLNRQDEFVQHMTARIIAKLACWHPQLMDKSDLHFYLSWLKDQLKT 643
E++ S + + +LL+ QD+ + ++ ++AKL HP L+ K + +L L +
Sbjct: 58 ESRGSMELLENCFSLLHAQDDTSKFVSLTMLAKLLNDHPNLIFKCWERMDMKFLDRLLLS 117
Query: 644 NNNDYI 661
+ +Y+
Sbjct: 118 THYEYV 123
>SPAC22F8.05 |||alpha,alpha-trehalose-phosphate synthase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 891
Score = 26.2 bits (55), Expect = 5.0
Identities = 8/25 (32%), Positives = 16/25 (64%)
Frame = -1
Query: 732 STERKAKRYSSIRSIICRHRATDWI 658
ST + +RYS+ ++ H A++W+
Sbjct: 580 STNERNQRYSNCLDVVLTHSASNWV 604
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 26.2 bits (55), Expect = 5.0
Identities = 13/45 (28%), Positives = 25/45 (55%)
Frame = -3
Query: 463 TKYLHPRFIFRKNVINEHKNVLNGVVLADMRQEVEKDFGTGIRIL 329
+KY P+F+F K ++E + + +G+ + M V + G I +L
Sbjct: 46 SKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFFENGKQIDML 90
>SPAC23C11.16 |plo1||Polo kinase Plo1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 683
Score = 26.2 bits (55), Expect = 5.0
Identities = 17/55 (30%), Positives = 28/55 (50%)
Frame = +2
Query: 428 LSEDKSRVKIFRETKFSGNVWQPFLNLLNRQDEFVQHMTARIIAKLACWHPQLMD 592
L DK+++K+F E K ++ P N++ D F +I +L C H LM+
Sbjct: 76 LQNDKTKLKLFGEIKVHQSMSHP--NIVGFIDCFEDSTNIYLILEL-CEHKSLME 127
>SPAC869.05c |||sulfate transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 840
Score = 25.8 bits (54), Expect = 6.6
Identities = 12/32 (37%), Positives = 17/32 (53%)
Frame = -1
Query: 237 QLI*VWRISLARICRTLVAAIMSIFSSPSVGI 142
Q I WR+ C ++ I+S+FSS GI
Sbjct: 492 QTILFWRLQPLEACIFFISVIVSVFSSIENGI 523
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 25.8 bits (54), Expect = 6.6
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = -2
Query: 398 EWCGPC*YASGG*ERLRHRHQ 336
+WCGPC Y E+L ++Q
Sbjct: 45 DWCGPCKYLKPFLEKLSEQNQ 65
>SPAC13C5.02 |dre4||DNA replication protein Dre4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 411
Score = 25.4 bits (53), Expect = 8.7
Identities = 16/37 (43%), Positives = 18/37 (48%), Gaps = 1/37 (2%)
Frame = +1
Query: 139 VNSNAR*RKNRHDCGHQRPTDPG-K*DTPNSD*LAVI 246
VN NA RKN D Q P P K PN+D V+
Sbjct: 62 VNENAEERKNSRDLRKQLPDRPKFKKRIPNNDSWVVV 98
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,028,243
Number of Sequences: 5004
Number of extensions: 58090
Number of successful extensions: 172
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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