BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30258
(607 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC11E3.07 |vma4||V-type ATPase subunit E|Schizosaccharomyces p... 76 4e-15
SPBC3B9.09 |vps36||RBZ zinc finger protein Vps36|Schizosaccharom... 28 0.92
SPBC14C8.04 |||acetolactate synthase regulatory unit|Schizosacch... 28 0.92
SPBC215.14c |vps20||vacuolar sorting protein Vps20|Schizosacchar... 26 3.7
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 25 6.5
SPBC215.10 |||haloacid dehalogenase-like hydrolase|Schizosacchar... 25 8.6
SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 25 8.6
>SPAC11E3.07 |vma4||V-type ATPase subunit E|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 227
Score = 76.2 bits (179), Expect = 4e-15
Identities = 44/156 (28%), Positives = 80/156 (51%)
Frame = +2
Query: 107 LSDADVQKQIKHMMAFIEQXXXXXXXXXXXXXXXXFNIEKGRLVQQQRLKIMEYSEKXEK 286
LSD VQ ++ M++FI+Q F +EK ++V++Q I + + K
Sbjct: 3 LSDEQVQAEMHKMVSFIKQEALEKAKEIHTLSEEEFQVEKAKIVREQCDAIDQTYDMKLK 62
Query: 287 QXELXKKIQSSNMLNQARLXXLKVREDHVRNVLDEXRKRLAEVPKDTKLYSELLVTLIVQ 466
+ + +KI SN+LN++RL L ++ + ++ K+L + + Y++ + LIVQ
Sbjct: 63 RASMAQKIAKSNVLNKSRLEILNSKQKVIDDIFSRVEKKLDGIEQKKDAYTKFMADLIVQ 122
Query: 467 ALFQLMEPTVTIRVRQTDKALVESLLGKAPTDYKNK 574
A+ L EP + RQ D +V++ + KA K+K
Sbjct: 123 AMELLGEPVGIVYSRQRDAEIVKAAIPKATEVLKSK 158
>SPBC3B9.09 |vps36||RBZ zinc finger protein
Vps36|Schizosaccharomyces pombe|chr 2|||Manual
Length = 467
Score = 28.3 bits (60), Expect = 0.92
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = -3
Query: 602 RLSTQHPS*SYSCSLLGLFRAGTPPEPCR 516
RLS +H S++C + G P PCR
Sbjct: 89 RLSLRHVEKSWACKICTFINVGDPINPCR 117
>SPBC14C8.04 |||acetolactate synthase regulatory
unit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 289
Score = 28.3 bits (60), Expect = 0.92
Identities = 16/45 (35%), Positives = 26/45 (57%), Gaps = 1/45 (2%)
Frame = +1
Query: 16 TSFPEPVINFLALYRPFFVLVVLKISSSHGAQRCRCSE-TDQAHD 147
T+ P+ V NFL+L RP+ VL + +S + +E T++A D
Sbjct: 233 TATPDRVDNFLSLLRPYGVLEACRTGTSAMTRAPHSNEVTEEAED 277
>SPBC215.14c |vps20||vacuolar sorting protein
Vps20|Schizosaccharomyces pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 3.7
Identities = 27/102 (26%), Positives = 50/102 (49%), Gaps = 4/102 (3%)
Frame = +2
Query: 236 VQQQRLKIMEYSEKXEKQXELXKKIQSSNMLN---QARLXXLKVREDHVRNVLDEXRKRL 406
+++QR K++ YS++ EK +L I + + + L LK ++ + ++ + +L
Sbjct: 18 IKEQRDKLLRYSKRLEKIEQLEIDIARKCLRDSDKRGALRALKAKKLY-SGLITQTYGQL 76
Query: 407 AEVPKDTKLYSELLVTLIVQ-ALFQLMEPTVTIRVRQTDKAL 529
+ +L S + TLI + +F L E T IR Q D L
Sbjct: 77 GNI---EQLLSTIEFTLIQKDVMFGLQEGTNLIRQLQADMPL 115
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 25.4 bits (53), Expect = 6.5
Identities = 17/49 (34%), Positives = 25/49 (51%)
Frame = -1
Query: 568 LVVCWGFSEQGLHQSLVGLTDADGDSGFHELEESLHNKCDQQL*VQFGV 422
L+V EQG ++ D+ +SG HE+ + L N DQQL Q +
Sbjct: 492 LIVACEELEQGFDLDIL---DSLRESGIHEVIQLLRNFPDQQLEKQLNI 537
>SPBC215.10 |||haloacid dehalogenase-like
hydrolase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 302
Score = 25.0 bits (52), Expect = 8.6
Identities = 14/42 (33%), Positives = 23/42 (54%)
Frame = +2
Query: 365 DHVRNVLDEXRKRLAEVPKDTKLYSELLVTLIVQALFQLMEP 490
D N L+E +KRLA +P D E+ +T + F+++ P
Sbjct: 181 DDDTNGLEEAKKRLAGIPSD-----EVALTQALPQTFEIIPP 217
>SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 780
Score = 25.0 bits (52), Expect = 8.6
Identities = 9/19 (47%), Positives = 11/19 (57%)
Frame = -1
Query: 106 RHGCCLFLTPLTRKTADIE 50
RHGCC+ L R DI+
Sbjct: 619 RHGCCILQRCLDRTNGDIQ 637
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,104,656
Number of Sequences: 5004
Number of extensions: 35521
Number of successful extensions: 101
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 99
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 101
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 266270664
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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