BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= maV30241 (333 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 26 1.3 SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomy... 25 2.3 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 25 2.3 SPAC1952.02 |||ribosome biogenesis protein|Schizosaccharomyces p... 25 4.0 SPCC1322.08 |srk1|mkp1|MAPK-activated protein kinase Srk1|Schizo... 24 5.3 SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces pom... 24 7.0 SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc... 23 9.2 SPBC32C12.02 |ste11|aff1, stex|transcription factor Ste11|Schizo... 23 9.2 SPBC19C7.11 |||ClC chloride channel |Schizosaccharomyces pombe|c... 23 9.2 SPBC15D4.07c |atg9|apg9|autophagy associated protein Atg9 |Schiz... 23 9.2 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 26.2 bits (55), Expect = 1.3 Identities = 10/21 (47%), Positives = 13/21 (61%) Frame = -2 Query: 131 HKFGSVTIRCASSLLFVSVPP 69 H GS+ +R S+LF VPP Sbjct: 2172 HASGSIALRITGSVLFALVPP 2192 >SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 2|||Manual Length = 786 Score = 25.4 bits (53), Expect = 2.3 Identities = 14/36 (38%), Positives = 20/36 (55%), Gaps = 3/36 (8%) Frame = +2 Query: 68 VAERSQTTRKKHIGSSPNQIYATS---KRLNHQRQH 166 V ++TT SS N ++ TS +RLNHQ +H Sbjct: 255 VDHNTRTTTWTRPASSTNPVHNTSSDSQRLNHQNRH 290 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 25.4 bits (53), Expect = 2.3 Identities = 13/27 (48%), Positives = 16/27 (59%) Frame = +3 Query: 3 LQHRLLVLSSPHLTRTVHSHLKWRNAH 83 L+H LL S L + + S LKWRN H Sbjct: 369 LRHELL---SAGLQKAIDSLLKWRNRH 392 >SPAC1952.02 |||ribosome biogenesis protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 202 Score = 24.6 bits (51), Expect = 4.0 Identities = 12/43 (27%), Positives = 23/43 (53%) Frame = +2 Query: 65 EVAERSQTTRKKHIGSSPNQIYATSKRLNHQRQHDEKRGQR*R 193 E ++R ++ +KK +++ SKRL H +KR ++ R Sbjct: 137 ESSKREKSKKKKEKKEKKDKLKKKSKRLKLDDSHTQKRKRKVR 179 >SPCC1322.08 |srk1|mkp1|MAPK-activated protein kinase Srk1|Schizosaccharomyces pombe|chr 3|||Manual Length = 580 Score = 24.2 bits (50), Expect = 5.3 Identities = 15/51 (29%), Positives = 22/51 (43%), Gaps = 1/51 (1%) Frame = +2 Query: 59 PPEVAERSQTTRKKHIGSSPNQIYATSKRLNHQRQHD-EKRGQR*RQGVQN 208 P +VA R + S +++ S + Q KRGQR QG+ N Sbjct: 451 PEDVAAAGSAARTPGVNSL-REVFNISYAAHRMEQEKIRKRGQRGNQGIMN 500 >SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces pombe|chr 1|||Manual Length = 419 Score = 23.8 bits (49), Expect = 7.0 Identities = 8/15 (53%), Positives = 11/15 (73%) Frame = -2 Query: 251 EGCAGF*REKLRSYG 207 +GC+ F +EK R YG Sbjct: 46 QGCSSFIKEKFRPYG 60 >SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 23.4 bits (48), Expect = 9.2 Identities = 11/50 (22%), Positives = 20/50 (40%) Frame = +2 Query: 2 FTASTTSVKFTSPHPHRS*PPEVAERSQTTRKKHIGSSPNQIYATSKRLN 151 F S P+P PP E + + SS N++++ + L+ Sbjct: 119 FNIPMPSASMFGPNPENPVPPSTEELANMLSNPMVQSSINEMFSNPQMLD 168 >SPBC32C12.02 |ste11|aff1, stex|transcription factor Ste11|Schizosaccharomyces pombe|chr 2|||Manual Length = 468 Score = 23.4 bits (48), Expect = 9.2 Identities = 10/32 (31%), Positives = 17/32 (53%) Frame = +2 Query: 71 AERSQTTRKKHIGSSPNQIYATSKRLNHQRQH 166 ++ S R+KH+ +P Y KR +R+H Sbjct: 62 SDLSALERQKHMLENPEYKYTPKKRSTVRRRH 93 >SPBC19C7.11 |||ClC chloride channel |Schizosaccharomyces pombe|chr 2|||Manual Length = 812 Score = 23.4 bits (48), Expect = 9.2 Identities = 11/20 (55%), Positives = 12/20 (60%) Frame = -2 Query: 215 SYGFALPAVIFAPSFRHAAS 156 SYG +PA IF PS AS Sbjct: 482 SYGAKVPAGIFVPSMAVGAS 501 >SPBC15D4.07c |atg9|apg9|autophagy associated protein Atg9 |Schizosaccharomyces pombe|chr 2|||Manual Length = 702 Score = 23.4 bits (48), Expect = 9.2 Identities = 9/15 (60%), Positives = 13/15 (86%) Frame = +1 Query: 214 LRSFSRQKPAQPSFN 258 L++ S+QKPA P+FN Sbjct: 115 LQNGSKQKPALPNFN 129 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,135,630 Number of Sequences: 5004 Number of extensions: 18955 Number of successful extensions: 55 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 55 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 55 length of database: 2,362,478 effective HSP length: 64 effective length of database: 2,042,222 effective search space used: 93942212 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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