Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30230
(591 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1039.07c |||4-aminobutyrate aminotransferase |Schizosaccharo... 74 1e-14
SPCC777.09c |arg1||acetylornithine aminotransferase|Schizosaccha... 70 2e-13
SPBC21C3.08c |||ornithine aminotransferase|Schizosaccharomyces p... 56 4e-09
SPCC417.11c |||glutamate-1-semialdehyde 2,1-aminomutaseaminotran... 52 5e-08
SPBC1773.03c |||aminotransferase class-III, unknown specificty|S... 42 5e-05
SPAC19D5.07 |uga1||4-aminobutyrate aminotransferase |Schizosacch... 42 7e-05
SPAC27F1.05c |||aminotransferase class-III, unknown specificty|S... 36 0.006
SPAC6B12.04c |||aminotransferase class I and II|Schizosaccharomy... 27 1.5
SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28 |Schizo... 27 2.7
SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein 2|S... 26 3.6
SPBC8D2.15 |||mitochondrial lipoic acid synthetase |Schizosaccha... 26 4.7
SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60... 25 6.2
SPBC4B4.04 |||translation initiation factor eIF2A |Schizosacchar... 25 6.2
SPBC530.04 |mod5||Tea1 anchoring protein Mod5|Schizosaccharomyce... 25 8.3
>SPAC1039.07c |||4-aminobutyrate aminotransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 448
Score = 74.1 bits (174), Expect = 1e-14
Identities = 35/124 (28%), Positives = 61/124 (49%)
Frame = +1
Query: 220 PVLLTQGHMQWLYDNDGKRYLDLFGGIVTVSVGHCHPKVNAALKDQLDVLWHTTNLYRHP 399
P ++ + +YD LD G ++ +GH HP + A ++ L L H + + P
Sbjct: 33 PKIIVRAKGCCVYDEQDNAILDFTSGQMSAILGHSHPDITACIEKNLPKLVHLFSGFLSP 92
Query: 400 KIYEYVEQLAAKLPGDLNVVYLVNSGSEANELATLLAKAYTGNLDIISLQTSYHGYTSSL 579
+ + +L+ LP L+ +++G EANE A +AK YT + ++ +S+HG T
Sbjct: 93 PVVQLATELSDLLPDGLDKTLFLSTGGEANEAALRMAKVYTNKYECVAFSSSWHGVTGGA 152
Query: 580 MGLT 591
LT
Sbjct: 153 ASLT 156
>SPCC777.09c |arg1||acetylornithine
aminotransferase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 441
Score = 70.1 bits (164), Expect = 2e-13
Identities = 43/144 (29%), Positives = 70/144 (48%), Gaps = 12/144 (8%)
Frame = +1
Query: 196 SITNAYKK-PVLLTQGHMQWLYDNDGKRYLDLFGGIVTVSVGHCHPKVNAALKDQLDVLW 372
+I + Y + PV+ +G +L+D +G++Y+D G+ S+GH HP+V DQ L
Sbjct: 46 NIISVYARYPVVAAKGEGSYLFDKEGRKYIDFTSGVAVTSLGHAHPEVARLAADQCSKLV 105
Query: 373 HTTNL-YRHP--KIYEYVEQLAAKLPG--DLNVVYLVNSGSEANELATLLA------KAY 519
H++NL Y P ++ + AK G ++ N G+EANE A A K
Sbjct: 106 HSSNLFYNEPAIELSNVINNSLAKNSGIAGPTKIFFANCGTEANETALKFARKAAFEKYG 165
Query: 520 TGNLDIISLQTSYHGYTSSLMGLT 591
G I+ S+HG + + +T
Sbjct: 166 EGKSQIVYFNNSFHGRSLGSLSIT 189
>SPBC21C3.08c |||ornithine aminotransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 438
Score = 56.0 bits (129), Expect = 4e-09
Identities = 38/155 (24%), Positives = 74/155 (47%), Gaps = 8/155 (5%)
Frame = +1
Query: 151 SYQQVEQMKGVYMPPSITNAYKKPVLLTQGHMQWLYDNDGKRYLDLFGGIVTVSVGHCHP 330
S +Q+E ++ Y + N + PV ++ ++D +G+ YLD V+ GHCHP
Sbjct: 12 STEQIEVLENEY---AAHNYHPLPVCFSKAKGAKVWDPEGREYLDFLSAYSAVNQGHCHP 68
Query: 331 KVNAALKDQLDVLWHTTNLYRHPKIYEYVEQLAAKLPGDLNVVYLVNSGSEANELATLLA 510
K+ AL +Q + ++ + + K + + + +V +N+G+EA E A LA
Sbjct: 69 KIIEALVEQAQRVTLSSRAFYNDKFGPFAKYITEYF--GYEMVIPMNTGAEAVETACKLA 126
Query: 511 KAY--------TGNLDIISLQTSYHGYTSSLMGLT 591
+ + T I+S ++HG T ++ ++
Sbjct: 127 RLWGYKAKKIPTDEAIILSCVDNFHGRTMGIISMS 161
>SPCC417.11c |||glutamate-1-semialdehyde
2,1-aminomutaseaminotransferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 435
Score = 52.4 bits (120), Expect = 5e-08
Identities = 31/115 (26%), Positives = 52/115 (45%)
Frame = +1
Query: 220 PVLLTQGHMQWLYDNDGKRYLDLFGGIVTVSVGHCHPKVNAALKDQLDVLWHTTNLYRHP 399
P+ + G+ L D DG Y D + GH +P + AL D + +
Sbjct: 52 PIFIEAGYGSKLRDVDGHEYTDFLNELTAGIYGHSNPVIKKALMQGFDEIGISLGGTTTC 111
Query: 400 KIYEYVEQLAAKLPGDLNVVYLVNSGSEANELATLLAKAYTGNLDIISLQTSYHG 564
++ Y E L ++ + + NSG+EAN A + A+ +TG +I++ YHG
Sbjct: 112 EL-NYAEALKSRFLS-IEKIRFCNSGTEANITAIIAARKFTGKRAVIAMHGGYHG 164
>SPBC1773.03c |||aminotransferase class-III, unknown
specificty|Schizosaccharomyces pombe|chr 2|||Manual
Length = 459
Score = 42.3 bits (95), Expect = 5e-05
Identities = 33/130 (25%), Positives = 55/130 (42%), Gaps = 7/130 (5%)
Frame = +1
Query: 202 TNAYKKPVLLTQGHMQWLYDNDGKRYLDLFGGIVTVSVGHCHPKVNAALKDQLDVLWHTT 381
T+ +P + + +LY DG R +D GG +GH + +V A+ Q + + +
Sbjct: 22 TSPNHRPPTVVRAEGVYLYLEDGTRIMDATGGAAVACLGHGNKEVIDAMHKQSEKVCYIH 81
Query: 382 NL-YRHPKIYEYVEQLAAKLPGDLNVVYLVNSGSEANELATLLAKAY------TGNLDII 540
++ + + + L ++ P Y NSGSEA E L Y II
Sbjct: 82 SMGFSNEPADKLANLLVSEHPDVFARAYFANSGSEAVETCLKLILQYWQLVGEKQRCHII 141
Query: 541 SLQTSYHGYT 570
+ + YHG T
Sbjct: 142 ARKQGYHGNT 151
>SPAC19D5.07 |uga1||4-aminobutyrate aminotransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 474
Score = 41.9 bits (94), Expect = 7e-05
Identities = 36/113 (31%), Positives = 55/113 (48%), Gaps = 5/113 (4%)
Frame = +1
Query: 205 NAYKKPVLLTQGHMQWLYDNDGKRYLDLFGGIVTVSVGHCHPKV-NAALKDQL-DVLWHT 378
+A K PV + +L D DG LD++ I T+ +G+ +P + AA D++ +L +
Sbjct: 47 SAVKFPVDYEKSIGNYLVDLDGNVLLDVYSQIATIPIGYNNPTLLKAAKSDEVATILMNR 106
Query: 379 TNLYRHP-KIYEYV--EQLAAKLPGDLNVVYLVNSGSEANELATLLAKAYTGN 528
L +P K + V E P VY SGS+ANE+A LA + N
Sbjct: 107 PALGNYPPKEWARVAYEGAIKYAPKGQKYVYFQMSGSDANEIAYKLAMLHHFN 159
>SPAC27F1.05c |||aminotransferase class-III, unknown
specificty|Schizosaccharomyces pombe|chr 1|||Manual
Length = 484
Score = 35.5 bits (78), Expect = 0.006
Identities = 30/116 (25%), Positives = 48/116 (41%), Gaps = 6/116 (5%)
Frame = +1
Query: 259 DNDGKRYLDLFGGIVTVSVGHCHPKVNAALKDQLDVLWHTTNLYRHPKI-YEYVEQLAAK 435
D G +LDL GG+ V+VG+ + V L+ D + + + + +A
Sbjct: 85 DEKGTEHLDLIGGVGVVTVGNNNQYVWDCLQKCFDAKLYMMGAISYRNLAAAFGRNMALL 144
Query: 436 LPGD-LNVVYLVNSGSEANE----LATLLAKAYTGNLDIISLQTSYHGYTSSLMGL 588
PG L + G+EANE L L + +S S+HG T+ + L
Sbjct: 145 SPGQKLTRTWTATGGAEANEGVIKLIRLATRYKPNKKKFLSTLNSFHGKTTGAVFL 200
>SPAC6B12.04c |||aminotransferase class I and II|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 421
Score = 27.5 bits (58), Expect = 1.5
Identities = 13/29 (44%), Positives = 19/29 (65%), Gaps = 2/29 (6%)
Frame = +1
Query: 46 EMANRGT--KLCFDIVRTYSTAKMPPTDF 126
E+ANR KLC+ I++ A +PPT+F
Sbjct: 355 EIANRPRDFKLCYWILKEIGVATIPPTEF 383
>SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1055
Score = 26.6 bits (56), Expect = 2.7
Identities = 12/30 (40%), Positives = 20/30 (66%)
Frame = +1
Query: 142 TGPSYQQVEQMKGVYMPPSITNAYKKPVLL 231
+G SY+ V+ + VY+ PS + A KKP ++
Sbjct: 977 SGDSYRTVKSNQTVYIHPSSSVAEKKPKVI 1006
>SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein
2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 513
Score = 26.2 bits (55), Expect = 3.6
Identities = 9/34 (26%), Positives = 16/34 (47%)
Frame = -2
Query: 338 FTFG*QWPTETVTIPPNRSRYLLPSLSYNHCMCP 237
+T+ +W E + P Y++ YN +CP
Sbjct: 108 YTYNSRWKIEIPSYPVREIPYVVADTPYNKLLCP 141
>SPBC8D2.15 |||mitochondrial lipoic acid synthetase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 370
Score = 25.8 bits (54), Expect = 4.7
Identities = 21/50 (42%), Positives = 27/50 (54%), Gaps = 3/50 (6%)
Frame = +1
Query: 388 YRHPKIYEYVEQLAAKLPGDLNVVY--LVNSGSEANE-LATLLAKAYTGN 528
Y HPK +EY +++A KL G L V LV S +A E L K +GN
Sbjct: 316 YVHPKKFEYWKEVAEKL-GFLYVASGPLVRSSYKAGEYFMENLIKKRSGN 364
>SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein
Hsp60|Schizosaccharomyces pombe|chr 1|||Manual
Length = 582
Score = 25.4 bits (53), Expect = 6.2
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = -2
Query: 155 YEGPVYGLGTKSVGGILAVLYVLTMSKHNFVPLLAISMCFSVEAIAIC 12
+E P+ L K V + +L L ++ PL+ I+ EA+A C
Sbjct: 246 FENPLILLSEKKVSAVQDILPSLELAAQQRRPLVIIAEDVDGEALAAC 293
>SPBC4B4.04 |||translation initiation factor eIF2A
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 576
Score = 25.4 bits (53), Expect = 6.2
Identities = 9/20 (45%), Positives = 11/20 (55%)
Frame = +2
Query: 209 PIRSRCFSPRGTCSGYTTTT 268
P R+ C+SP G Y T T
Sbjct: 36 PARNACYSPNGKLFAYATAT 55
>SPBC530.04 |mod5||Tea1 anchoring protein Mod5|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 522
Score = 25.0 bits (52), Expect = 8.3
Identities = 12/34 (35%), Positives = 15/34 (44%)
Frame = +1
Query: 121 DFVPRPYTGPSYQQVEQMKGVYMPPSITNAYKKP 222
D P P PS Q + MPP +T Y +P
Sbjct: 454 DGSPDPPAAPSIQNSLSVHESEMPPHVTRDYTQP 487
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,657,328
Number of Sequences: 5004
Number of extensions: 57623
Number of successful extensions: 160
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 155
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 157
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 256184654
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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