BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= maV30223 (727 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC364.06 |nap1||nucleosome assembly protein Nap1 |Schizosaccha... 96 4e-21 SPBC2D10.11c |||nucleosome assembly protein Nap2 |Schizosaccharo... 74 2e-14 SPAC1783.01 |||FAD binding protein|Schizosaccharomyces pombe|chr... 28 1.2 SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 26 4.8 SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 26 6.3 SPAC13G6.03 |gpi7||GPI anchor biosynthesis protein Gpi7 |Schizos... 26 6.3 SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 8.3 SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 25 8.3 >SPCC364.06 |nap1||nucleosome assembly protein Nap1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 393 Score = 96.3 bits (229), Expect = 4e-21 Identities = 62/178 (34%), Positives = 87/178 (48%) Frame = +2 Query: 194 LHAEAMASLPPNVRRRIRALRTLQKEFVDIEAKFYSEVHAXXXXXXXXXXXXXXXRALIV 373 L +E ++ LP V+RRI LR LQK + D+E++F E+ R+ +V Sbjct: 63 LTSEGVSELPEAVQRRISGLRGLQKRYSDLESQFQKELFELEKAYAKKYAPIFKRRSEVV 122 Query: 374 NGTYEPNDDECLNPWRDDTEEEELARAVQNAAITEGEEKKDDKAIEPPMDPNVKGIPDFW 553 G EP ++E ++ E A + T E KK + + KGIP+FW Sbjct: 123 RGADEPTEEEI--------KKGEAADENEKKEPTSSESKKQEGG------DDTKGIPEFW 168 Query: 554 YNIFRNVSMLSEMMQEHDEPILKCLQDIKVQMHEDPISFTLEFYFAPNEYFTNTVLTK 727 +NV LSEM+ DE L L DI++ E P F LEF FA N +FTN +LTK Sbjct: 169 LTAMKNVLSLSEMITPEDEGALSHLVDIRISYMEKP-GFKLEFEFAENPFFTNKILTK 225 >SPBC2D10.11c |||nucleosome assembly protein Nap2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 73.7 bits (173), Expect = 2e-14 Identities = 66/202 (32%), Positives = 91/202 (45%), Gaps = 9/202 (4%) Frame = +2 Query: 149 SGVTRNE--MIAAITNRLHAEA------MASLPPNVRRRIRALRTLQKEFVDIEAKFYSE 304 SG+ RN M++ I RL + + SL P V+ RI AL+ LQK+ I+ +F + Sbjct: 56 SGLLRNNPAMLSMIEGRLSSLVGKSSGYIESLAPAVQNRITALKGLQKDCDAIQYEFRQK 115 Query: 305 VHAXXXXXXXXXXXXXXXRALIVNGTYEPNDDECLNPWRDDTEEEELARAVQNAAITEGE 484 + RA I+ G EP DDE D EEE QN Sbjct: 116 MLDLETKYEKKYQPIFSRRAEIIKGVSEPVDDEL------DHEEE----IFQNNL----- 160 Query: 485 EKKDDKAIEPPMDPNVKGIPDFWYNIFRNVSMLSEMMQEHDEPILKCLQDIK-VQMHEDP 661 P+ KGIP+FW NV ++ EM+ DE +L+ L DI+ + D Sbjct: 161 -------------PDPKGIPEFWLTCLHNVFLVGEMITPEDENVLRSLSDIRFTNLSGDV 207 Query: 662 ISFTLEFYFAPNEYFTNTVLTK 727 + LEF F N+YFTN +LTK Sbjct: 208 HGYKLEFEFDSNDYFTNKILTK 229 >SPAC1783.01 |||FAD binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 583 Score = 28.3 bits (60), Expect = 1.2 Identities = 16/40 (40%), Positives = 21/40 (52%) Frame = +1 Query: 178 GHHKSPSCRSDGIPTPECSSANPRLENSSEGVCRH*GQVL 297 GHHK+P CR+ + S N R E + V RH G V+ Sbjct: 485 GHHKNPKCRAKKLVV---ESRNGRREYVQDAVRRH-GDVI 520 >SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 726 Score = 26.2 bits (55), Expect = 4.8 Identities = 12/29 (41%), Positives = 18/29 (62%) Frame = +2 Query: 458 QNAAITEGEEKKDDKAIEPPMDPNVKGIP 544 +N+ + E EEK D +AIE + +V G P Sbjct: 554 KNSLVNEAEEKNDLEAIEAAKNFHVNGKP 582 >SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2100 Score = 25.8 bits (54), Expect = 6.3 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = +2 Query: 137 HLLKSGVTRNEMIAAITNRLHAEAMASLP 223 HLL++ T +E AA +LH + + S P Sbjct: 1596 HLLRNSATNDETKAAFVYQLHKQGILSEP 1624 >SPAC13G6.03 |gpi7||GPI anchor biosynthesis protein Gpi7 |Schizosaccharomyces pombe|chr 1|||Manual Length = 758 Score = 25.8 bits (54), Expect = 6.3 Identities = 16/55 (29%), Positives = 32/55 (58%), Gaps = 4/55 (7%) Frame = -1 Query: 532 YIGIH-WRLDSLVILLFLTLSDGSI---LYRPS*LFFFSVITPWVETFIIIRFIC 380 Y G+ + + ++ ILLF ++ G++ L++P + SV T W+ + I + F+C Sbjct: 659 YTGLDSYNIFAVGILLFTSVFAGALWWCLHQPKRMMDRSVKTFWIMSSISLTFLC 713 >SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1038 Score = 25.4 bits (53), Expect = 8.3 Identities = 10/36 (27%), Positives = 21/36 (58%) Frame = +2 Query: 434 EEELARAVQNAAITEGEEKKDDKAIEPPMDPNVKGI 541 EEEL+ ++ N + E++KD+ +P P++ + Sbjct: 475 EEELSDSLSNDFGIDAEKEKDENLSKPEHHPSITSV 510 >SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1583 Score = 25.4 bits (53), Expect = 8.3 Identities = 11/23 (47%), Positives = 13/23 (56%) Frame = +1 Query: 505 YRASNGSQCKGYPRLLVQHIQEC 573 YR SNG + Y L V+ IQ C Sbjct: 489 YRLSNGKSIQYYSTLFVRLIQSC 511 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,932,424 Number of Sequences: 5004 Number of extensions: 57602 Number of successful extensions: 201 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 193 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 198 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 341222980 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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