Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30192
(644 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g20360.1 68415.m02377 expressed protein 135 2e-32
At1g19540.1 68414.m02434 isoflavone reductase, putative similar ... 38 0.004
At1g75290.1 68414.m08746 isoflavone reductase, putative similar ... 33 0.16
At1g75280.1 68414.m08745 isoflavone reductase, putative identica... 33 0.16
At2g44040.1 68415.m05475 dihydrodipicolinate reductase family pr... 32 0.28
At1g75300.1 68414.m08747 isoflavone reductase, putative identica... 32 0.28
At2g45310.1 68415.m05639 NAD-dependent epimerase/dehydratase fam... 31 0.87
At5g10730.1 68418.m01243 expressed protein 30 1.5
At5g13490.1 68418.m01556 ADP, ATP carrier protein 2, mitochondri... 29 2.0
At3g59890.2 68416.m06683 dihydrodipicolinate reductase family pr... 29 2.0
At3g59890.1 68416.m06684 dihydrodipicolinate reductase family pr... 29 2.0
At3g03980.1 68416.m00419 short-chain dehydrogenase/reductase (SD... 29 2.6
At2g34070.1 68415.m04171 expressed protein 29 3.5
At5g19060.1 68418.m02266 expressed protein ; expression supporte... 28 4.6
At4g11370.1 68417.m01833 zinc finger (C3HC4-type RING finger) fa... 28 4.6
At3g46810.1 68416.m05081 DC1 domain-containing protein contains ... 28 6.1
At1g16930.1 68414.m02053 F-box family protein contains F-box dom... 28 6.1
At1g02000.1 68414.m00118 NAD-dependent epimerase/dehydratase fam... 28 6.1
>At2g20360.1 68415.m02377 expressed protein
Length = 402
Score = 135 bits (327), Expect = 2e-32
Identities = 71/173 (41%), Positives = 113/173 (65%), Gaps = 1/173 (0%)
Frame = +3
Query: 126 HLNGSMSVVYIKAANYSSDRKPNLAAYKRGTGGRSSFNGIVATVFGCTGFVGRYVCNKLG 305
HLNG+ + Y +++ ++ +LA ++GTGGRSS +GIVATVFG TGF+GRY+ +L
Sbjct: 34 HLNGTDNCRY--SSSLATKGVGHLA--RKGTGGRSSVSGIVATVFGATGFLGRYLVQQLA 89
Query: 306 KIGTQLILPYRGDFYDAQRLKVCGDLGQVLFTPYHLLDEESIAKAVRYSNVVINLVGRDY 485
K+G+Q+++P+RG + LK+ GDLGQV+ + DE+SI + +NVVINL+GR+Y
Sbjct: 90 KMGSQVLVPFRGSEDSPRHLKLMGDLGQVVPMKFDPRDEDSIKAVMAKANVVINLIGREY 149
Query: 486 ETKNFKYNDVHVDGVRRIARICREE-GVERFIHLSYLNAEEHPKPLVLKKPSA 641
ET+NF + D + ++A + +E G+ R+I +S L A +L+ +A
Sbjct: 150 ETRNFSFEDANHHIAEKLALVAKEHGGIMRYIQVSCLGASVSSPSRMLRAKAA 202
>At1g19540.1 68414.m02434 isoflavone reductase, putative similar to
SP|P52577; contains isoflavone reductase domain PF02716
Length = 310
Score = 38.3 bits (85), Expect = 0.004
Identities = 29/104 (27%), Positives = 51/104 (49%), Gaps = 4/104 (3%)
Frame = +3
Query: 255 VFGCTGFVGRYVCNKLGKIGTQLILPYR----GDFYDAQRLKVCGDLGQVLFTPYHLLDE 422
V G TG +G+ + + K G R D AQ ++ DLG + L D+
Sbjct: 7 VIGATGLIGKVLVEESAKSGHATFALVREASLSDPVKAQLVERFKDLGVTILYG-SLSDK 65
Query: 423 ESIAKAVRYSNVVINLVGRDYETKNFKYNDVHVDGVRRIARICR 554
ES+ KA++ +VVI+ VGR ++T+ ++ +D ++ + R
Sbjct: 66 ESLVKAIKQVDVVISAVGR-FQTEILNQTNI-IDAIKESGNVKR 107
>At1g75290.1 68414.m08746 isoflavone reductase, putative similar to
SP|P52577 Isoflavone reductase homolog P3 (EC 1.3.1.-)
{Arabidopsis thaliana}; contains Pfam profile PF02716:
Isoflavone reductase
Length = 323
Score = 33.1 bits (72), Expect = 0.16
Identities = 24/77 (31%), Positives = 36/77 (46%), Gaps = 3/77 (3%)
Frame = +3
Query: 255 VFGCTGFVGRYVCNKLGKIG-TQLILPYRGDFYDAQRLKVCGDLGQVLFTPYH--LLDEE 425
V G TG +G+ + K G + L L D + K + T H L D E
Sbjct: 10 VIGGTGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTLLHGDLNDHE 69
Query: 426 SIAKAVRYSNVVINLVG 476
S+ KA++ ++VVI+ VG
Sbjct: 70 SLVKAIKQADVVISTVG 86
>At1g75280.1 68414.m08745 isoflavone reductase, putative identical
to SP|P52577 Isoflavone reductase homolog P3 (EC
1.3.1.-) {Arabidopsis thaliana}; contains Pfam profile
PF02716: isoflavone reductase
Length = 310
Score = 33.1 bits (72), Expect = 0.16
Identities = 23/77 (29%), Positives = 36/77 (46%), Gaps = 3/77 (3%)
Frame = +3
Query: 255 VFGCTGFVGRYVCNKLGKIG-TQLILPYRGDFYDAQRLKVCGDLGQVLFTPYH--LLDEE 425
V G TG++G+++ K G + L D + K + T H L D E
Sbjct: 10 VIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHGDLNDHE 69
Query: 426 SIAKAVRYSNVVINLVG 476
S+ KA++ +VVI+ VG
Sbjct: 70 SLVKAIKQVDVVISTVG 86
>At2g44040.1 68415.m05475 dihydrodipicolinate reductase family
protein weak similarity to SP|Q52419 Dihydrodipicolinate
reductase (EC 1.3.1.26) (DHPR) {Pseudomonas syringae} ;
contains Pfam profiles PF01113: Dihydrodipicolinate
reductase N-terminus, PF05173: Dihydrodipicolinate
reductase C-terminus
Length = 347
Score = 32.3 bits (70), Expect = 0.28
Identities = 16/48 (33%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Frame = +3
Query: 237 NGIVATVFGCTGFVGRYVCNKLGKIGTQLI-LPYRGDFYDAQRLKVCG 377
NGI V GC+G +G+ V G ++ + + D QR++VCG
Sbjct: 71 NGISIMVNGCSGKMGKAVIKAADSAGVNIVPISFGSAGEDGQRVEVCG 118
>At1g75300.1 68414.m08747 isoflavone reductase, putative identical
to SP|P52577 Isoflavone reductase homolog P3 (EC
1.3.1.-) {Arabidopsis thaliana}; contains Pfam profile
PF02716: Isoflavone reductase
Length = 322
Score = 32.3 bits (70), Expect = 0.28
Identities = 23/78 (29%), Positives = 37/78 (47%), Gaps = 4/78 (5%)
Frame = +3
Query: 255 VFGCTGFVGRYVCNKLGKIGTQLILPYR----GDFYDAQRLKVCGDLGQVLFTPYHLLDE 422
V G TG++G ++ K G R D ++ ++ DLG V L D
Sbjct: 10 VIGGTGYMGEFIVEGSAKAGNPTFALVREASLSDPVKSKTIQSFKDLG-VTILHGDLNDH 68
Query: 423 ESIAKAVRYSNVVINLVG 476
ES+ KA++ +VVI+ +G
Sbjct: 69 ESLVKAIKQVDVVISTIG 86
>At2g45310.1 68415.m05639 NAD-dependent epimerase/dehydratase family
protein similar to nucleotide sugar epimerase from
Vibrio vulnificus GI:3093975 [PID:g3093975], WbnF
[Escherichia coli] GI:5739472, CAPI protein
{Staphylococcus aureus} SP|P39858; contains Pfam profile
PF01370 NAD dependent epimerase/dehydratase family
Length = 437
Score = 30.7 bits (66), Expect = 0.87
Identities = 18/43 (41%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
Frame = +3
Query: 228 SSFNGIVATVFGCTGFVGRYVCNKLGKIGTQLI-LPYRGDFYD 353
S+ NGI V G GFVG +V L + G +I L D+YD
Sbjct: 92 STNNGITVLVTGAAGFVGTHVSAALKRRGDGVIGLDNFNDYYD 134
>At5g10730.1 68418.m01243 expressed protein
Length = 287
Score = 29.9 bits (64), Expect = 1.5
Identities = 28/111 (25%), Positives = 48/111 (43%)
Frame = +3
Query: 255 VFGCTGFVGRYVCNKLGKIGTQLILPYRGDFYDAQRLKVCGDLGQVLFTPYHLLDEESIA 434
V G GFVG +VC + G + R Q +V + +LL + +
Sbjct: 61 VLGGNGFVGSHVCKEALDRGLSVSSLSRSGRSSLQE----SWASRVTWHQGNLLSSDLLK 116
Query: 435 KAVRYSNVVINLVGRDYETKNFKYNDVHVDGVRRIARICREEGVERFIHLS 587
A+ VI+ VG + + ++ Y + I R E+GV+RF+++S
Sbjct: 117 DALEGVTSVISCVG-GFGSNSYMYKINGTANINAI-RAASEKGVKRFVYIS 165
>At5g13490.1 68418.m01556 ADP, ATP carrier protein 2, mitochondrial
/ ADP/ATP translocase 2 / adenine nucleotide
translocator 2 (ANT2) identical to SWISS-PROT:P40941
ADP,ATP carrier protein 2, mitochondrial precursor
(Adenine nucleotide translocator 2) [Arabidopsis
thaliana]
Length = 385
Score = 29.5 bits (63), Expect = 2.0
Identities = 26/100 (26%), Positives = 44/100 (44%), Gaps = 7/100 (7%)
Frame = +3
Query: 135 GSMSVVYIKAANYSSDRKPNLAAYKRGTGGRSSFNGIV---ATVFGCTGFVGRYVCNKLG 305
G+ S++++ + +Y+ R N + + GG FNG+V G G Y +
Sbjct: 200 GASSLLFVYSLDYARTRLANDSKSAKKGGGERQFNGLVDVYKKTLKSDGIAGLYRGFNIS 259
Query: 306 KIGTQLILPYRG---DFYDAQR-LKVCGDLGQVLFTPYHL 413
G I+ YRG YD+ + + + GDL F + L
Sbjct: 260 CAG---IIVYRGLYFGLYDSVKPVLLTGDLQDSFFASFAL 296
>At3g59890.2 68416.m06683 dihydrodipicolinate reductase family
protein weak similarity to SP|Q52419 Dihydrodipicolinate
reductase (EC 1.3.1.26) (DHPR) {Pseudomonas syringae};
contains Pfam profiles PF01113: Dihydrodipicolinate
reductase N-terminus, PF05173: Dihydrodipicolinate
reductase C-terminus
Length = 343
Score = 29.5 bits (63), Expect = 2.0
Identities = 16/48 (33%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +3
Query: 237 NGIVATVFGCTGFVGRYVCNKLGKIGTQLILPYRGDFYDA-QRLKVCG 377
NGI V GC+G +G+ V G ++ G +A Q ++VCG
Sbjct: 67 NGISIMVNGCSGKMGKAVIKAADSAGVNIVPTSFGSVEEAGQTVEVCG 114
>At3g59890.1 68416.m06684 dihydrodipicolinate reductase family
protein weak similarity to SP|Q52419 Dihydrodipicolinate
reductase (EC 1.3.1.26) (DHPR) {Pseudomonas syringae};
contains Pfam profiles PF01113: Dihydrodipicolinate
reductase N-terminus, PF05173: Dihydrodipicolinate
reductase C-terminus
Length = 349
Score = 29.5 bits (63), Expect = 2.0
Identities = 16/48 (33%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +3
Query: 237 NGIVATVFGCTGFVGRYVCNKLGKIGTQLILPYRGDFYDA-QRLKVCG 377
NGI V GC+G +G+ V G ++ G +A Q ++VCG
Sbjct: 73 NGISIMVNGCSGKMGKAVIKAADSAGVNIVPTSFGSVEEAGQTVEVCG 120
>At3g03980.1 68416.m00419 short-chain dehydrogenase/reductase (SDR)
family protein similar to short-chain type
dehydrogenase/reductase SP:Q08632 [Picea abies]
Length = 270
Score = 29.1 bits (62), Expect = 2.6
Identities = 13/42 (30%), Positives = 24/42 (57%)
Frame = +3
Query: 240 GIVATVFGCTGFVGRYVCNKLGKIGTQLILPYRGDFYDAQRL 365
G VA V G + +GR + L ++G ++++ Y DA+R+
Sbjct: 16 GRVAIVTGSSRGIGRAIAIHLAELGARIVINYTSKAADAERV 57
>At2g34070.1 68415.m04171 expressed protein
Length = 385
Score = 28.7 bits (61), Expect = 3.5
Identities = 19/55 (34%), Positives = 27/55 (49%), Gaps = 1/55 (1%)
Frame = +3
Query: 129 LNGS-MSVVYIKAANYSSDRKPNLAAYKRGTGGRSSFNGIVATVFGCTGFVGRYV 290
L+G+ ++ Y+K + S K LAA GG+ G T GC F GR+V
Sbjct: 20 LSGADQALAYVKKPHVSQRNKTALAAVAGRGGGKEMLKGRKQT-SGCNLFQGRWV 73
>At5g19060.1 68418.m02266 expressed protein ; expression supported
by MPSS
Length = 551
Score = 28.3 bits (60), Expect = 4.6
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = -3
Query: 246 RCR*SYCGHQYLAYMLQGWVYDPNCS*RL 160
RCR +C A GWVY +CS +L
Sbjct: 272 RCREPWCDGALSALESNGWVYSSHCSFKL 300
>At4g11370.1 68417.m01833 zinc finger (C3HC4-type RING finger)
family protein strong similarity to RING-H2 finger
protein RHA1a [Arabidopsis thaliana] GI:3790554;
contains Pfam profile PF00097: Zinc finger, C3HC4 type
(RING finger)
Length = 159
Score = 28.3 bits (60), Expect = 4.6
Identities = 23/81 (28%), Positives = 33/81 (40%), Gaps = 2/81 (2%)
Frame = -3
Query: 411 DGKE*TGPGLNLRTLSTFGHHRNRLC-MVKLTGYQFFPI-CCTHSVRQIRCNRKLWLRCR 238
D E + P L LST N L +V+ + P CCT + + K+ +
Sbjct: 45 DHNETSAPDLTRHALSTSASLANELIPVVRFSDLPTDPEDCCTVCLSDFESDDKVRQLPK 104
Query: 237 *SYCGHQYLAYMLQGWVYDPN 175
CGH + Y L W+ D N
Sbjct: 105 ---CGHVFHHYCLDRWIVDYN 122
>At3g46810.1 68416.m05081 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 686
Score = 27.9 bits (59), Expect = 6.1
Identities = 16/66 (24%), Positives = 28/66 (42%)
Frame = +3
Query: 270 GFVGRYVCNKLGKIGTQLILPYRGDFYDAQRLKVCGDLGQVLFTPYHLLDEESIAKAVRY 449
GF G Y C+K + R D +D L+ G + P+ ++DE +I
Sbjct: 328 GFYGAYSCSKCSSYVVHSLCATRKDVWDMVELE--GTPEEEEIAPFEVVDENTIKHMSHD 385
Query: 450 SNVVIN 467
+++ N
Sbjct: 386 HDLIFN 391
>At1g16930.1 68414.m02053 F-box family protein contains F-box domain
Pfam:PF00646
Length = 449
Score = 27.9 bits (59), Expect = 6.1
Identities = 18/58 (31%), Positives = 31/58 (53%), Gaps = 3/58 (5%)
Frame = +3
Query: 411 LLDEESIAKAV---RYSNVVINLVGRDYETKNFKYNDVHVDGVRRIARICREEGVERF 575
L +ES+ +V R+ N+ +++ D ++ NF +DV V V R E+ +ERF
Sbjct: 32 LSTKESVCTSVLSKRWRNLWLHVPVLDLDSNNFPDDDVFVSFVNRFLGSENEQHLERF 89
>At1g02000.1 68414.m00118 NAD-dependent epimerase/dehydratase family
protein similar to nucleotide sugar epimerase from
Vibrio vulnificus GI:3093975 [PID:g3093975], WbnF
[Escherichia coli] GI:5739472, CAPI protein
{Staphylococcus aureus} SP|P39858; contains Pfam profile
PF01370 NAD dependent epimerase/dehydratase family
Length = 434
Score = 27.9 bits (59), Expect = 6.1
Identities = 15/40 (37%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
Frame = +3
Query: 237 NGIVATVFGCTGFVGRYVCNKLGKIGTQLI-LPYRGDFYD 353
NG+ V G GFVG +V L + G ++ L D+YD
Sbjct: 90 NGVSVLVTGAAGFVGTHVSAALKRRGDGVLGLDNFNDYYD 129
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,241,448
Number of Sequences: 28952
Number of extensions: 265161
Number of successful extensions: 683
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 651
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 682
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1334473344
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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