BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= maV30187 (710 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC2D10.09 |||3-hydroxyisobutyryl-CoA hydrolase|Schizosaccharom... 53 5e-08 SPAC1782.08c |rex3||exonuclease Rex3 |Schizosaccharomyces pombe|... 29 0.87 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 27 2.0 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 27 2.0 SPBC1604.19c |||TRAPP complex subunit Trs85 |Schizosaccharomyces... 27 3.5 SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr... 27 3.5 SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 26 4.6 SPAC1296.03c |sxa2||serine carboxypeptidase Sxa2|Schizosaccharom... 26 6.1 SPCC550.11 |||karyopherin|Schizosaccharomyces pombe|chr 3|||Manual 25 8.1 SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 25 8.1 SPBC543.08 |||phosphoinositide biosynthesis protein |Schizosacch... 25 8.1 SPAC139.06 |hat1|SPAC23C4.01|histone acetyltransferase Hat1|Schi... 25 8.1 SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccha... 25 8.1 >SPBC2D10.09 |||3-hydroxyisobutyryl-CoA hydrolase|Schizosaccharomyces pombe|chr 2|||Manual Length = 429 Score = 52.8 bits (121), Expect = 5e-08 Identities = 45/175 (25%), Positives = 80/175 (45%), Gaps = 4/175 (2%) Frame = +3 Query: 189 NGVYVVTLDSPNVKVNSLNTQVMEEVSNIVNEIETNSGIEAAVIISGKPGCFIAGADISM 368 NG + TL+ P V +N++N +++ + + +E S + +I+ G F +G DI Sbjct: 64 NGARIFTLNRPKV-LNAINVDMIDSILPKLVSLE-ESNLAKVIILKGNGRSFSSGGDIKA 121 Query: 369 ----IENCKTKEEVVSLSKRGHEIFRRIEQSRKPYIAAIQGSCLGGGLETALACKYRIAV 536 I++ K E + ++ + + + +KP +A + G +GGG A+ +RIA Sbjct: 122 AALSIQDGKLPEVRHAFAQE-YRLSHTLATYQKPVVALMNGITMGGGSGLAMHVPFRIAC 180 Query: 537 KDSKTGFGLPEVMLGLLPGGGGTQRLPALTSIPTTLDLALTGKTVKADKAKKLGI 701 +D T F +PE +G + L T L LT + VK + GI Sbjct: 181 ED--TMFAMPETGIGYFTDVAASFFFSRLPGYFGTY-LGLTSQIVKGYDCLRTGI 232 >SPAC1782.08c |rex3||exonuclease Rex3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 540 Score = 28.7 bits (61), Expect = 0.87 Identities = 15/47 (31%), Positives = 24/47 (51%) Frame = +3 Query: 168 HTKCKLVNGVYVVTLDSPNVKVNSLNTQVMEEVSNIVNEIETNSGIE 308 + CKL V +VT P V +LN + + +S +N +TN I+ Sbjct: 63 YDNCKLSICVPIVTTCIPYHSVENLNFNIRQNISTTLNLEKTNDSIK 109 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 27.5 bits (58), Expect = 2.0 Identities = 17/42 (40%), Positives = 19/42 (45%) Frame = -2 Query: 616 GNLCVPPPPGKSPNMTSGSPNPVLESFTAMRYLHARAVSNPP 491 G PPPP +S T P P+ S RAVSNPP Sbjct: 357 GRSAPPPPPPRSAPSTGRQPPPLSSS---------RAVSNPP 389 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 27.5 bits (58), Expect = 2.0 Identities = 22/94 (23%), Positives = 42/94 (44%) Frame = +3 Query: 225 VKVNSLNTQVMEEVSNIVNEIETNSGIEAAVIISGKPGCFIAGADISMIENCKTKEEVVS 404 V+ + + Q +++ N + + A + I P G S + + T E VS Sbjct: 534 VRGDDSDVQTGYVLTSTKNPVHATTRFIAQIAILELPSILTTG--YSCVMHIHTAVEEVS 591 Query: 405 LSKRGHEIFRRIEQSRKPYIAAIQGSCLGGGLET 506 +K H++ + +S+KP + A +G + LET Sbjct: 592 FAKLLHKLDKTNRKSKKPPMFATKGMKIIAELET 625 >SPBC1604.19c |||TRAPP complex subunit Trs85 |Schizosaccharomyces pombe|chr 2|||Manual Length = 658 Score = 26.6 bits (56), Expect = 3.5 Identities = 15/59 (25%), Positives = 27/59 (45%) Frame = +2 Query: 467 RNTRQLSRRWIRDCSCM*ISHCCERLQNRIWTTRSHVGTFARRWRDTEITCSHLHTYNF 643 RNT + W SC I C +QNR + +S + ++ +W D + + ++F Sbjct: 467 RNTFSQDKAWNYLASCEEIQIICLLMQNRNISLKSQI-SYLNKWFDEMVYIYAVRLHSF 524 >SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr 3|||Manual Length = 512 Score = 26.6 bits (56), Expect = 3.5 Identities = 24/76 (31%), Positives = 38/76 (50%), Gaps = 2/76 (2%) Frame = -2 Query: 538 FTAMRYLHARAVSNPPPRQLPCIAAIYGFRDCSILLNISCPLLDRL--TTSSFVLQFSIM 365 +TA+ +H + PPP+ LP I I R CS L + P+L +L T + VL+ M Sbjct: 254 YTAVPTIHQILLRTPPPKPLPRIRFI---RSCSSPL--APPVLSKLEATFRAPVLEAYAM 308 Query: 364 LISAPAMKHPGLPDIM 317 ++ M LP ++ Sbjct: 309 TEASHQMTTNPLPPLV 324 >SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 821 Score = 26.2 bits (55), Expect = 4.6 Identities = 12/40 (30%), Positives = 18/40 (45%) Frame = -2 Query: 601 PPPPGKSPNMTSGSPNPVLESFTAMRYLHARAVSNPPPRQ 482 P P G S + S P SF + +L + SN P ++ Sbjct: 140 PRPSGNSSRLDRNSSRPFTSSFHSAPFLSSYGSSNDPSQE 179 >SPAC1296.03c |sxa2||serine carboxypeptidase Sxa2|Schizosaccharomyces pombe|chr 1|||Manual Length = 507 Score = 25.8 bits (54), Expect = 6.1 Identities = 20/66 (30%), Positives = 32/66 (48%) Frame = -3 Query: 633 VWR*EQVISVSLHRRAKVPT*LLVVQILFWSLSQQCDIYMQEQSLIHLLDSCLVLRQYTV 454 +WR E S S R + + +F S+S CD+Y L++L +SC++ Y V Sbjct: 294 IWRPEYNFSTSTSLRKREALDGEDIGNVFNSISG-CDLYSLSNFLLYLENSCVI--TYDV 350 Query: 453 SVIVRF 436 S+ F Sbjct: 351 SLDCSF 356 >SPCC550.11 |||karyopherin|Schizosaccharomyces pombe|chr 3|||Manual Length = 1029 Score = 25.4 bits (53), Expect = 8.1 Identities = 15/46 (32%), Positives = 23/46 (50%) Frame = -2 Query: 448 DCSILLNISCPLLDRLTTSSFVLQFSIMLISAPAMKHPGLPDIMTA 311 D S LLN +L+ L + ++ L P M+H + D+MTA Sbjct: 499 DKSQLLNAYQAVLNCLQDNDLPVRIQAALALQPLMRHLEVHDVMTA 544 >SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces pombe|chr 1|||Manual Length = 670 Score = 25.4 bits (53), Expect = 8.1 Identities = 26/120 (21%), Positives = 61/120 (50%), Gaps = 3/120 (2%) Frame = +3 Query: 66 NSKILSALKILRSRKELFISGVHSRKYAVPASQVHTKCKLVNGVYVVTLDSPNVK-VNSL 242 NS + L L+S + GV +R+ + + + L + V +T + +++ +N L Sbjct: 384 NSTVYQELLNLKSNNSS-VDGVKTRRQLLEENAL-----LSHKVLKLTEEIQDLETLNQL 437 Query: 243 NTQVMEEVSNIVNEIETNSGIEAAVIISGKPGCFIAGADISMIENC--KTKEEVVSLSKR 416 NT++ S +NE++ + + ++IS +P A ++ ++ C ++EE++ L+ + Sbjct: 438 NTEIEARQSEKLNEVQEETQRLSQLLISSQP----ALTEVKHLKLCLSDSQEELLQLNAK 493 >SPBC543.08 |||phosphoinositide biosynthesis protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 250 Score = 25.4 bits (53), Expect = 8.1 Identities = 9/20 (45%), Positives = 14/20 (70%) Frame = +2 Query: 164 STYKMQIGQWSLRSHFGLSK 223 S Y + + +WS+ S+FG SK Sbjct: 38 SIYSIYVDKWSITSYFGNSK 57 >SPAC139.06 |hat1|SPAC23C4.01|histone acetyltransferase Hat1|Schizosaccharomyces pombe|chr 1|||Manual Length = 378 Score = 25.4 bits (53), Expect = 8.1 Identities = 7/18 (38%), Positives = 13/18 (72%) Frame = -2 Query: 463 IYGFRDCSILLNISCPLL 410 IYG++D ++ + CPL+ Sbjct: 45 IYGYKDLNVTITYECPLM 62 >SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccharomyces pombe|chr 3|||Manual Length = 1284 Score = 25.4 bits (53), Expect = 8.1 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = -2 Query: 610 LCVPPPPGKSPNMTSGSPNPVLESFTAMR 524 L P PP + P +T+ SPN ++ S +R Sbjct: 240 LTPPAPPVELPPVTTTSPNAIIRSVQWIR 268 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,802,033 Number of Sequences: 5004 Number of extensions: 60677 Number of successful extensions: 224 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 207 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 224 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 331187010 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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