BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= maV30186 (693 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pomb... 117 2e-27 SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro... 52 8e-08 SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|... 48 1e-06 SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz... 48 1e-06 SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi... 48 1e-06 SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 48 1e-06 SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch... 48 1e-06 SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc... 46 5e-06 SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual 33 0.052 SPBC6B1.06c |ubp14|ucp2|ubiquitin C-terminal hydrolase Ubp14|Sch... 31 0.21 SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 29 0.84 SPAC56F8.08 |mud1|ucp1, ddi1|UBA domain protein Mud1|Schizosacch... 27 1.9 SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 27 3.4 SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces p... 27 3.4 SPBC13A2.02 |||nucleoporin Nup82|Schizosaccharomyces pombe|chr 2... 26 4.5 SPBC1706.01 |tea4|wsh3|tip elongation aberrant protein Tea4|Schi... 26 4.5 SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pomb... 25 7.9 SPAC328.03 |tps1||alpha,alpha-trehalose-phosphate synthase [UDP-... 25 7.9 >SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pombe|chr 2|||Manual Length = 368 Score = 117 bits (281), Expect = 2e-27 Identities = 68/188 (36%), Positives = 102/188 (54%), Gaps = 13/188 (6%) Frame = +3 Query: 93 MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272 M +T K LQQQ F I D K +LK +++ +++ + Q+LIY+G+IL DD + Sbjct: 1 MNLTFKNLQQQKFVIS-DVSADTKISELKEKIQTQQNYEVERQKLIYSGRILADDKTVGE 59 Query: 273 YNIDEKKFIVIMVTKPKVSESQPTSTSIPEAGESASTDSGDXXXXXXXXXXXXXXXXXXX 452 YNI E+ FIV MV++PK S S P S + P AS Sbjct: 60 YNIKEQDFIVCMVSRPKTSTSTPKSAASPAPNPPASVPEKKVEAPSSTVAESTSTTQTVA 119 Query: 453 XRVTETPNT--TSDAELEA-----------TIQSIMDMGYDRPQVEQALRASFNNRERAV 593 P+T TS+A ++A ++++++MGY+R +VE+A+RA+FNN +RAV Sbjct: 120 AAAPSNPDTTATSEAPIDANTLAVGAQRNVAVENMVEMGYERSEVERAMRAAFNNPDRAV 179 Query: 594 EYLITGIP 617 EYL+TGIP Sbjct: 180 EYLLTGIP 187 >SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like protein modifier Ned8|Schizosaccharomyces pombe|chr 2|||Manual Length = 78 Score = 52.0 bits (119), Expect = 8e-08 Identities = 24/64 (37%), Positives = 40/64 (62%) Frame = +3 Query: 93 MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272 ML+ +KTL + +++IDP + V +K ++E ++G QRLIYAGK + DD S Sbjct: 1 MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEG--IPPSQQRLIYAGKQMADDKNAES 58 Query: 273 YNID 284 Y+++ Sbjct: 59 YHLE 62 >SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 48.4 bits (110), Expect = 1e-06 Identities = 24/73 (32%), Positives = 45/73 (61%) Frame = +3 Query: 93 MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272 M + +KTL +T +E++ +T+ +K KI+ ++G D QRLI+AGK L D ++ Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEG--IPPDQQRLIFAGKQLEDGRTLSD 58 Query: 273 YNIDEKKFIVIMV 311 YNI ++ + +++ Sbjct: 59 YNIQKESTLHLVL 71 >SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 48.4 bits (110), Expect = 1e-06 Identities = 24/73 (32%), Positives = 45/73 (61%) Frame = +3 Query: 93 MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272 M + +KTL +T +E++ +T+ +K KI+ ++G D QRLI+AGK L D ++ Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEG--IPPDQQRLIFAGKQLEDGRTLSD 58 Query: 273 YNIDEKKFIVIMV 311 YNI ++ + +++ Sbjct: 59 YNIQKESTLHLVL 71 >SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 48.4 bits (110), Expect = 1e-06 Identities = 24/73 (32%), Positives = 45/73 (61%) Frame = +3 Query: 93 MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272 M + +KTL +T +E++ +T+ +K KI+ ++G D QRLI+AGK L D ++ Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEG--IPPDQQRLIFAGKQLEDGRTLSD 58 Query: 273 YNIDEKKFIVIMV 311 YNI ++ + +++ Sbjct: 59 YNIQKESTLHLVL 71 >SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||Manual Length = 382 Score = 48.4 bits (110), Expect = 1e-06 Identities = 24/73 (32%), Positives = 45/73 (61%) Frame = +3 Query: 93 MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272 M + +KTL +T +E++ +T+ +K KI+ ++G D QRLI+AGK L D ++ Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEG--IPPDQQRLIFAGKQLEDGRTLSD 58 Query: 273 YNIDEKKFIVIMV 311 YNI ++ + +++ Sbjct: 59 YNIQKESTLHLVL 71 Score = 48.4 bits (110), Expect = 1e-06 Identities = 24/73 (32%), Positives = 45/73 (61%) Frame = +3 Query: 93 MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272 M + +KTL +T +E++ +T+ +K KI+ ++G D QRLI+AGK L D ++ Sbjct: 77 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEG--IPPDQQRLIFAGKQLEDGRTLSD 134 Query: 273 YNIDEKKFIVIMV 311 YNI ++ + +++ Sbjct: 135 YNIQKESTLHLVL 147 Score = 48.4 bits (110), Expect = 1e-06 Identities = 24/73 (32%), Positives = 45/73 (61%) Frame = +3 Query: 93 MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272 M + +KTL +T +E++ +T+ +K KI+ ++G D QRLI+AGK L D ++ Sbjct: 153 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEG--IPPDQQRLIFAGKQLEDGRTLSD 210 Query: 273 YNIDEKKFIVIMV 311 YNI ++ + +++ Sbjct: 211 YNIQKESTLHLVL 223 Score = 48.4 bits (110), Expect = 1e-06 Identities = 24/73 (32%), Positives = 45/73 (61%) Frame = +3 Query: 93 MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272 M + +KTL +T +E++ +T+ +K KI+ ++G D QRLI+AGK L D ++ Sbjct: 229 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEG--IPPDQQRLIFAGKQLEDGRTLSD 286 Query: 273 YNIDEKKFIVIMV 311 YNI ++ + +++ Sbjct: 287 YNIQKESTLHLVL 299 Score = 48.4 bits (110), Expect = 1e-06 Identities = 24/73 (32%), Positives = 45/73 (61%) Frame = +3 Query: 93 MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272 M + +KTL +T +E++ +T+ +K KI+ ++G D QRLI+AGK L D ++ Sbjct: 305 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEG--IPPDQQRLIFAGKQLEDGRTLSD 362 Query: 273 YNIDEKKFIVIMV 311 YNI ++ + +++ Sbjct: 363 YNIQKESTLHLVL 375 >SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 48.4 bits (110), Expect = 1e-06 Identities = 24/73 (32%), Positives = 45/73 (61%) Frame = +3 Query: 93 MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272 M + +KTL +T +E++ +T+ +K KI+ ++G D QRLI+AGK L D ++ Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEG--IPPDQQRLIFAGKQLEDGRTLSD 58 Query: 273 YNIDEKKFIVIMV 311 YNI ++ + +++ Sbjct: 59 YNIQKESTLHLVL 71 >SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 46.0 bits (104), Expect = 5e-06 Identities = 26/85 (30%), Positives = 44/85 (51%) Frame = +3 Query: 99 VTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINSYN 278 +T+K Q + + +D E +V ALK I D + QRLIYAG++L D+ + +Y Sbjct: 6 LTIKAANDQKYAVTVDSESSVLALKEAIAPVA--DIEKERQRLIYAGRVLKDEESLKTYK 63 Query: 279 IDEKKFIVIMVTKPKVSESQPTSTS 353 I + I ++ T + + T+ S Sbjct: 64 IQDGHSIHLVKTLGQNPAAAATNVS 88 >SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 32.7 bits (71), Expect = 0.052 Identities = 17/51 (33%), Positives = 25/51 (49%) Frame = +3 Query: 243 ILLDDNKINSYNIDEKKFIVIMVTKPKVSESQPTSTSIPEAGESASTDSGD 395 +L + INS + D KK+ M P ++ Q T + EA S T SG+ Sbjct: 426 VLQKTDPINSRSFDPKKYTASMEILPPENKRQRTEKGVDEASNSTLTKSGN 476 >SPBC6B1.06c |ubp14|ucp2|ubiquitin C-terminal hydrolase Ubp14|Schizosaccharomyces pombe|chr 2|||Manual Length = 775 Score = 30.7 bits (66), Expect = 0.21 Identities = 13/36 (36%), Positives = 22/36 (61%) Frame = +3 Query: 498 EATIQSIMDMGYDRPQVEQALRASFNNRERAVEYLI 605 E +QS+ + G+ Q + L S NN ERAV++++ Sbjct: 642 EENVQSLCEFGFTVAQARKGLLESNNNIERAVDWIL 677 >SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccharomyces pombe|chr 1|||Manual Length = 433 Score = 28.7 bits (61), Expect = 0.84 Identities = 11/30 (36%), Positives = 19/30 (63%) Frame = +3 Query: 207 VADHQRLIYAGKILLDDNKINSYNIDEKKF 296 V D+ +IY G+++++ NK NID + F Sbjct: 85 VPDNGLVIYCGEVIMEGNKTRKLNIDFEPF 114 >SPAC56F8.08 |mud1|ucp1, ddi1|UBA domain protein Mud1|Schizosaccharomyces pombe|chr 1|||Manual Length = 332 Score = 27.5 bits (58), Expect = 1.9 Identities = 13/44 (29%), Positives = 24/44 (54%) Frame = +3 Query: 474 NTTSDAELEATIQSIMDMGYDRPQVEQALRASFNNRERAVEYLI 605 N +D L + I ++ MG+D + QAL A+ + + A +L+ Sbjct: 289 NPPTDPGLNSKIAQLVSMGFDPLEAAQALDAANGDLDVAASFLL 332 >SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|Schizosaccharomyces pombe|chr 1|||Manual Length = 2397 Score = 26.6 bits (56), Expect = 3.4 Identities = 22/88 (25%), Positives = 39/88 (44%), Gaps = 4/88 (4%) Frame = +3 Query: 108 KTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLI---YAGKILLDDNKINSY- 275 K Q+ + IDPE + + ++KG D +AD + + +I++ I+ Y Sbjct: 1421 KRSAQEWANLNIDPEADLLIFVGRWTLQKGIDLIADITPTLLENFNSQIVVVGPVIDLYG 1480 Query: 276 NIDEKKFIVIMVTKPKVSESQPTSTSIP 359 +KF +M P S+P T +P Sbjct: 1481 KFAAEKFTALMKKYPGRIYSRPLFTQLP 1508 >SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1323 Score = 26.6 bits (56), Expect = 3.4 Identities = 20/97 (20%), Positives = 38/97 (39%) Frame = +3 Query: 105 LKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINSYNID 284 ++ LQ + P ++V I+ E + D + L Y K +N + YN+D Sbjct: 1 MRELQGDDSSRKSPPSDSVVKNSSPIDYEHSLKSLQDERTLNYPNKQFNSENPSSYYNMD 60 Query: 285 EKKFIVIMVTKPKVSESQPTSTSIPEAGESASTDSGD 395 + + + ES +S + +S + GD Sbjct: 61 DSGELCNFELEKDSLESMIHESSTALSAQSNTAQDGD 97 >SPBC13A2.02 |||nucleoporin Nup82|Schizosaccharomyces pombe|chr 2|||Manual Length = 803 Score = 26.2 bits (55), Expect = 4.5 Identities = 13/34 (38%), Positives = 19/34 (55%) Frame = +3 Query: 288 KKFIVIMVTKPKVSESQPTSTSIPEAGESASTDS 389 K IVI+ + K+SE P + S+ GES + S Sbjct: 103 KSKIVILGLRSKLSEQNPLAESVSNFGESVNNFS 136 >SPBC1706.01 |tea4|wsh3|tip elongation aberrant protein Tea4|Schizosaccharomyces pombe|chr 2|||Manual Length = 809 Score = 26.2 bits (55), Expect = 4.5 Identities = 12/45 (26%), Positives = 22/45 (48%) Frame = +3 Query: 468 TPNTTSDAELEATIQSIMDMGYDRPQVEQALRASFNNRERAVEYL 602 T +TS + + I S + YD +Q++ A NN +V ++ Sbjct: 674 TEESTSSSSFSSLITSPASLQYDENPFKQSVVAELNNNSSSVPFV 718 >SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pombe|chr 2|||Manual Length = 741 Score = 25.4 bits (53), Expect = 7.9 Identities = 12/24 (50%), Positives = 15/24 (62%) Frame = +3 Query: 318 PKVSESQPTSTSIPEAGESASTDS 389 P + S PT+ S P AGE AS D+ Sbjct: 252 PDSNTSTPTTRSEPLAGEGASIDA 275 >SPAC328.03 |tps1||alpha,alpha-trehalose-phosphate synthase [UDP-forming]|Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 25.4 bits (53), Expect = 7.9 Identities = 17/57 (29%), Positives = 30/57 (52%) Frame = +3 Query: 126 TFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINSYNIDEKKF 296 TF I IDPE+ ALK + KD +A +R + K+++ ++++ +KF Sbjct: 253 TFPIGIDPEKFSDALKSDVV----KDRIASIERRLQGVKVIVGVDRLDYIKGVPQKF 305 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,414,995 Number of Sequences: 5004 Number of extensions: 43644 Number of successful extensions: 177 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 158 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 174 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 321951680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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