SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= maV30186
         (693 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pomb...   117   2e-27
SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro...    52   8e-08
SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|...    48   1e-06
SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz...    48   1e-06
SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi...    48   1e-06
SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M...    48   1e-06
SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch...    48   1e-06
SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc...    46   5e-06
SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual          33   0.052
SPBC6B1.06c |ubp14|ucp2|ubiquitin C-terminal hydrolase Ubp14|Sch...    31   0.21 
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha...    29   0.84 
SPAC56F8.08 |mud1|ucp1, ddi1|UBA domain protein Mud1|Schizosacch...    27   1.9  
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S...    27   3.4  
SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces p...    27   3.4  
SPBC13A2.02 |||nucleoporin Nup82|Schizosaccharomyces pombe|chr 2...    26   4.5  
SPBC1706.01 |tea4|wsh3|tip elongation aberrant protein Tea4|Schi...    26   4.5  
SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pomb...    25   7.9  
SPAC328.03 |tps1||alpha,alpha-trehalose-phosphate synthase [UDP-...    25   7.9  

>SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 368

 Score =  117 bits (281), Expect = 2e-27
 Identities = 68/188 (36%), Positives = 102/188 (54%), Gaps = 13/188 (6%)
 Frame = +3

Query: 93  MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272
           M +T K LQQQ F I  D     K  +LK +++  +++  + Q+LIY+G+IL DD  +  
Sbjct: 1   MNLTFKNLQQQKFVIS-DVSADTKISELKEKIQTQQNYEVERQKLIYSGRILADDKTVGE 59

Query: 273 YNIDEKKFIVIMVTKPKVSESQPTSTSIPEAGESASTDSGDXXXXXXXXXXXXXXXXXXX 452
           YNI E+ FIV MV++PK S S P S + P     AS                        
Sbjct: 60  YNIKEQDFIVCMVSRPKTSTSTPKSAASPAPNPPASVPEKKVEAPSSTVAESTSTTQTVA 119

Query: 453 XRVTETPNT--TSDAELEA-----------TIQSIMDMGYDRPQVEQALRASFNNRERAV 593
                 P+T  TS+A ++A            ++++++MGY+R +VE+A+RA+FNN +RAV
Sbjct: 120 AAAPSNPDTTATSEAPIDANTLAVGAQRNVAVENMVEMGYERSEVERAMRAAFNNPDRAV 179

Query: 594 EYLITGIP 617
           EYL+TGIP
Sbjct: 180 EYLLTGIP 187


>SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like
           protein modifier Ned8|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 78

 Score = 52.0 bits (119), Expect = 8e-08
 Identities = 24/64 (37%), Positives = 40/64 (62%)
 Frame = +3

Query: 93  MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272
           ML+ +KTL  +  +++IDP + V  +K ++E ++G       QRLIYAGK + DD    S
Sbjct: 1   MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEG--IPPSQQRLIYAGKQMADDKNAES 58

Query: 273 YNID 284
           Y+++
Sbjct: 59  YHLE 62


>SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein
           Ubi2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 128

 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 24/73 (32%), Positives = 45/73 (61%)
 Frame = +3

Query: 93  MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272
           M + +KTL  +T  +E++  +T+  +K KI+ ++G     D QRLI+AGK L D   ++ 
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEG--IPPDQQRLIFAGKQLEDGRTLSD 58

Query: 273 YNIDEKKFIVIMV 311
           YNI ++  + +++
Sbjct: 59  YNIQKESTLHLVL 71


>SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein
           Ubi1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 128

 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 24/73 (32%), Positives = 45/73 (61%)
 Frame = +3

Query: 93  MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272
           M + +KTL  +T  +E++  +T+  +K KI+ ++G     D QRLI+AGK L D   ++ 
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEG--IPPDQQRLIFAGKQLEDGRTLSD 58

Query: 273 YNIDEKKFIVIMV 311
           YNI ++  + +++
Sbjct: 59  YNIQKESTLHLVL 71


>SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein
           Ubi3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 150

 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 24/73 (32%), Positives = 45/73 (61%)
 Frame = +3

Query: 93  MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272
           M + +KTL  +T  +E++  +T+  +K KI+ ++G     D QRLI+AGK L D   ++ 
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEG--IPPDQQRLIFAGKQLEDGRTLSD 58

Query: 273 YNIDEKKFIVIMV 311
           YNI ++  + +++
Sbjct: 59  YNIQKESTLHLVL 71


>SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 382

 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 24/73 (32%), Positives = 45/73 (61%)
 Frame = +3

Query: 93  MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272
           M + +KTL  +T  +E++  +T+  +K KI+ ++G     D QRLI+AGK L D   ++ 
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEG--IPPDQQRLIFAGKQLEDGRTLSD 58

Query: 273 YNIDEKKFIVIMV 311
           YNI ++  + +++
Sbjct: 59  YNIQKESTLHLVL 71



 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 24/73 (32%), Positives = 45/73 (61%)
 Frame = +3

Query: 93  MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272
           M + +KTL  +T  +E++  +T+  +K KI+ ++G     D QRLI+AGK L D   ++ 
Sbjct: 77  MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEG--IPPDQQRLIFAGKQLEDGRTLSD 134

Query: 273 YNIDEKKFIVIMV 311
           YNI ++  + +++
Sbjct: 135 YNIQKESTLHLVL 147



 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 24/73 (32%), Positives = 45/73 (61%)
 Frame = +3

Query: 93  MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272
           M + +KTL  +T  +E++  +T+  +K KI+ ++G     D QRLI+AGK L D   ++ 
Sbjct: 153 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEG--IPPDQQRLIFAGKQLEDGRTLSD 210

Query: 273 YNIDEKKFIVIMV 311
           YNI ++  + +++
Sbjct: 211 YNIQKESTLHLVL 223



 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 24/73 (32%), Positives = 45/73 (61%)
 Frame = +3

Query: 93  MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272
           M + +KTL  +T  +E++  +T+  +K KI+ ++G     D QRLI+AGK L D   ++ 
Sbjct: 229 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEG--IPPDQQRLIFAGKQLEDGRTLSD 286

Query: 273 YNIDEKKFIVIMV 311
           YNI ++  + +++
Sbjct: 287 YNIQKESTLHLVL 299



 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 24/73 (32%), Positives = 45/73 (61%)
 Frame = +3

Query: 93  MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272
           M + +KTL  +T  +E++  +T+  +K KI+ ++G     D QRLI+AGK L D   ++ 
Sbjct: 305 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEG--IPPDQQRLIFAGKQLEDGRTLSD 362

Query: 273 YNIDEKKFIVIMV 311
           YNI ++  + +++
Sbjct: 363 YNIQKESTLHLVL 375


>SPAC589.10c |||ribomal-ubiquitin fusion protein
           Ubi5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 150

 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 24/73 (32%), Positives = 45/73 (61%)
 Frame = +3

Query: 93  MLVTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINS 272
           M + +KTL  +T  +E++  +T+  +K KI+ ++G     D QRLI+AGK L D   ++ 
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEG--IPPDQQRLIFAGKQLEDGRTLSD 58

Query: 273 YNIDEKKFIVIMV 311
           YNI ++  + +++
Sbjct: 59  YNIQKESTLHLVL 71


>SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 354

 Score = 46.0 bits (104), Expect = 5e-06
 Identities = 26/85 (30%), Positives = 44/85 (51%)
 Frame = +3

Query: 99  VTLKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINSYN 278
           +T+K    Q + + +D E +V ALK  I      D   + QRLIYAG++L D+  + +Y 
Sbjct: 6   LTIKAANDQKYAVTVDSESSVLALKEAIAPVA--DIEKERQRLIYAGRVLKDEESLKTYK 63

Query: 279 IDEKKFIVIMVTKPKVSESQPTSTS 353
           I +   I ++ T  +   +  T+ S
Sbjct: 64  IQDGHSIHLVKTLGQNPAAAATNVS 88


>SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 674

 Score = 32.7 bits (71), Expect = 0.052
 Identities = 17/51 (33%), Positives = 25/51 (49%)
 Frame = +3

Query: 243 ILLDDNKINSYNIDEKKFIVIMVTKPKVSESQPTSTSIPEAGESASTDSGD 395
           +L   + INS + D KK+   M   P  ++ Q T   + EA  S  T SG+
Sbjct: 426 VLQKTDPINSRSFDPKKYTASMEILPPENKRQRTEKGVDEASNSTLTKSGN 476


>SPBC6B1.06c |ubp14|ucp2|ubiquitin C-terminal hydrolase
           Ubp14|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 775

 Score = 30.7 bits (66), Expect = 0.21
 Identities = 13/36 (36%), Positives = 22/36 (61%)
 Frame = +3

Query: 498 EATIQSIMDMGYDRPQVEQALRASFNNRERAVEYLI 605
           E  +QS+ + G+   Q  + L  S NN ERAV++++
Sbjct: 642 EENVQSLCEFGFTVAQARKGLLESNNNIERAVDWIL 677


>SPAC1834.01 |sup45||translation release factor
           eRF1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 433

 Score = 28.7 bits (61), Expect = 0.84
 Identities = 11/30 (36%), Positives = 19/30 (63%)
 Frame = +3

Query: 207 VADHQRLIYAGKILLDDNKINSYNIDEKKF 296
           V D+  +IY G+++++ NK    NID + F
Sbjct: 85  VPDNGLVIYCGEVIMEGNKTRKLNIDFEPF 114


>SPAC56F8.08 |mud1|ucp1, ddi1|UBA domain protein
           Mud1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 332

 Score = 27.5 bits (58), Expect = 1.9
 Identities = 13/44 (29%), Positives = 24/44 (54%)
 Frame = +3

Query: 474 NTTSDAELEATIQSIMDMGYDRPQVEQALRASFNNRERAVEYLI 605
           N  +D  L + I  ++ MG+D  +  QAL A+  + + A  +L+
Sbjct: 289 NPPTDPGLNSKIAQLVSMGFDPLEAAQALDAANGDLDVAASFLL 332


>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
            Mok11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2397

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 22/88 (25%), Positives = 39/88 (44%), Gaps = 4/88 (4%)
 Frame = +3

Query: 108  KTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLI---YAGKILLDDNKINSY- 275
            K   Q+   + IDPE  +     +  ++KG D +AD    +   +  +I++    I+ Y 
Sbjct: 1421 KRSAQEWANLNIDPEADLLIFVGRWTLQKGIDLIADITPTLLENFNSQIVVVGPVIDLYG 1480

Query: 276  NIDEKKFIVIMVTKPKVSESQPTSTSIP 359
                +KF  +M   P    S+P  T +P
Sbjct: 1481 KFAAEKFTALMKKYPGRIYSRPLFTQLP 1508


>SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1323

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 20/97 (20%), Positives = 38/97 (39%)
 Frame = +3

Query: 105 LKTLQQQTFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINSYNID 284
           ++ LQ      +  P ++V      I+ E     + D + L Y  K    +N  + YN+D
Sbjct: 1   MRELQGDDSSRKSPPSDSVVKNSSPIDYEHSLKSLQDERTLNYPNKQFNSENPSSYYNMD 60

Query: 285 EKKFIVIMVTKPKVSESQPTSTSIPEAGESASTDSGD 395
           +   +     +    ES    +S   + +S +   GD
Sbjct: 61  DSGELCNFELEKDSLESMIHESSTALSAQSNTAQDGD 97


>SPBC13A2.02 |||nucleoporin Nup82|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 803

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 13/34 (38%), Positives = 19/34 (55%)
 Frame = +3

Query: 288 KKFIVIMVTKPKVSESQPTSTSIPEAGESASTDS 389
           K  IVI+  + K+SE  P + S+   GES +  S
Sbjct: 103 KSKIVILGLRSKLSEQNPLAESVSNFGESVNNFS 136


>SPBC1706.01 |tea4|wsh3|tip elongation aberrant protein
           Tea4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 809

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 12/45 (26%), Positives = 22/45 (48%)
 Frame = +3

Query: 468 TPNTTSDAELEATIQSIMDMGYDRPQVEQALRASFNNRERAVEYL 602
           T  +TS +   + I S   + YD    +Q++ A  NN   +V ++
Sbjct: 674 TEESTSSSSFSSLITSPASLQYDENPFKQSVVAELNNNSSSVPFV 718


>SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 741

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 12/24 (50%), Positives = 15/24 (62%)
 Frame = +3

Query: 318 PKVSESQPTSTSIPEAGESASTDS 389
           P  + S PT+ S P AGE AS D+
Sbjct: 252 PDSNTSTPTTRSEPLAGEGASIDA 275


>SPAC328.03 |tps1||alpha,alpha-trehalose-phosphate synthase
           [UDP-forming]|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 513

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 17/57 (29%), Positives = 30/57 (52%)
 Frame = +3

Query: 126 TFQIEIDPEETVKALKLKIEVEKGKDFVADHQRLIYAGKILLDDNKINSYNIDEKKF 296
           TF I IDPE+   ALK  +     KD +A  +R +   K+++  ++++      +KF
Sbjct: 253 TFPIGIDPEKFSDALKSDVV----KDRIASIERRLQGVKVIVGVDRLDYIKGVPQKF 305


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,414,995
Number of Sequences: 5004
Number of extensions: 43644
Number of successful extensions: 177
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -