BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= maV30179 (703 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyc... 29 0.64 SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 28 1.5 SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|... 28 1.5 SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 27 2.6 SPBC1709.13c |||lysine methyltransferase |Schizosaccharomyces po... 27 2.6 SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 26 4.5 SPBC3F6.04c |||U3 snoRNP protein Nop14 |Schizosaccharomyces pomb... 26 4.5 SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyce... 26 4.5 SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces pomb... 26 6.0 SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 26 6.0 SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schiz... 26 6.0 SPAC343.09 |ubx3|mug39|UBX domain protein Ubx3|Schizosaccharomyc... 26 6.0 SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosac... 26 6.0 SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccha... 25 7.9 SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosacc... 25 7.9 SPCC777.04 |||amino acid transporter |Schizosaccharomyces pombe|... 25 7.9 SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 25 7.9 SPCC5E4.03c |taf72||transcription factor TFIID complex subunit 5... 25 7.9 >SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 29.1 bits (62), Expect = 0.64 Identities = 23/71 (32%), Positives = 38/71 (53%), Gaps = 4/71 (5%) Frame = +2 Query: 440 LDIPKVKKQSLQHLK-SSVEEDF-QFLSELAA-LKAVTEKVE-SGAISADNIIDFYNLRI 607 L+I K KK K ++V D Q+ E + ++ EK++ + + +N +F+NL+ Sbjct: 546 LEIEKRKKYETNEAKITTVATDLSQYYRESKEYIASLYEKLDRTERNNKENENNFWNLKF 605 Query: 608 NSLHALRDFHG 640 N L LR FHG Sbjct: 606 NLLTMLRSFHG 616 >SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1958 Score = 27.9 bits (59), Expect = 1.5 Identities = 17/39 (43%), Positives = 22/39 (56%) Frame = +2 Query: 86 SFSGSSKTFESLLKEIFSASLGLSVEENSEWNGLLITDP 202 SF S +SLL E+FSA LG +++ WN LL P Sbjct: 32 SFLWSESAKKSLLNEVFSALLGY---DHTLWNTLLPERP 67 >SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|Schizosaccharomyces pombe|chr 2|||Manual Length = 1173 Score = 27.9 bits (59), Expect = 1.5 Identities = 19/65 (29%), Positives = 35/65 (53%), Gaps = 4/65 (6%) Frame = -2 Query: 207 LNGSVINNPFHSEFSSTE-RPREAENISFKRLSKVLLEPEKLNDSGLW---RMLSSPEAL 40 L G ++NPF S+F + E R EAE I ++ + ++ ++ +W RM++S + Sbjct: 298 LLGDEVHNPF-SDFETVEDRAHEAEFIEKQKKHLSIEASDRFKENSMWEKNRMITSGVSK 356 Query: 39 IPIIE 25 P +E Sbjct: 357 APGLE 361 >SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr 1|||Manual Length = 1778 Score = 27.1 bits (57), Expect = 2.6 Identities = 14/45 (31%), Positives = 24/45 (53%) Frame = -3 Query: 200 DQLSIIHSIRSSLQRKGQEKQKIFLLKDSRKFYWNQRNLTTQDYG 66 DQL++ + ++ E Q LK+SR Y+N+ +L T +G Sbjct: 1126 DQLNLSVQRSALIKAAFPESQSNANLKNSRGIYYNEHDLVTDIFG 1170 >SPBC1709.13c |||lysine methyltransferase |Schizosaccharomyces pombe|chr 2|||Manual Length = 547 Score = 27.1 bits (57), Expect = 2.6 Identities = 23/86 (26%), Positives = 39/86 (45%), Gaps = 4/86 (4%) Frame = +2 Query: 119 LLKEIFSASLGLSVEE---NSEWNGLLITDPFNTPEAVVEVYISGISS-LGSSADFKSKK 286 LL+ A L ++E +++ L+ TD + ++ VY IS L S + Sbjct: 425 LLQHPLFAPLSHAIESLYGSTDAEALVATDEQDILMILICVYCLSISEKLPFSISMLVEG 484 Query: 287 YPLVVDEYEPDTFDVLKHRINQRFTN 364 YP V + + F++L Q+FTN Sbjct: 485 YPAVANPEGVEVFEILDEMFFQQFTN 510 >SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 3699 Score = 26.2 bits (55), Expect = 4.5 Identities = 13/33 (39%), Positives = 18/33 (54%) Frame = +2 Query: 77 ESLSFSGSSKTFESLLKEIFSASLGLSVEENSE 175 E L SGS K FE+ + F+ L + V +N E Sbjct: 119 ELLKKSGSYKGFEAFVNRTFAVLLRIVVNDNEE 151 >SPBC3F6.04c |||U3 snoRNP protein Nop14 |Schizosaccharomyces pombe|chr 2|||Manual Length = 827 Score = 26.2 bits (55), Expect = 4.5 Identities = 13/40 (32%), Positives = 19/40 (47%) Frame = +2 Query: 68 HSPESLSFSGSSKTFESLLKEIFSASLGLSVEENSEWNGL 187 + +S S +GS + E +F GL E EWNG+ Sbjct: 321 YQEDSASEAGSIED-EQATDNVFGFGKGLEQENEEEWNGI 359 >SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 2104 Score = 26.2 bits (55), Expect = 4.5 Identities = 16/42 (38%), Positives = 24/42 (57%) Frame = +2 Query: 551 VESGAISADNIIDFYNLRINSLHALRDFHGPNSLPTKEAKKL 676 V+SG +++D I Y+ +L L+D SL TKEA +L Sbjct: 1099 VKSGPLNSDENI--YSTSSTTLSILKDVQELKSLHTKEANQL 1138 >SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 398 Score = 25.8 bits (54), Expect = 6.0 Identities = 17/54 (31%), Positives = 29/54 (53%) Frame = +2 Query: 488 SVEEDFQFLSELAALKAVTEKVESGAISADNIIDFYNLRINSLHALRDFHGPNS 649 SV++D FLSE+ ALK + E + S + + ++ L AL + +G +S Sbjct: 166 SVKQDRVFLSEIYALKILVEFLSSKSQQERSDTSIVIGQLVGLEALYEKYGKDS 219 >SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 1|||Manual Length = 632 Score = 25.8 bits (54), Expect = 6.0 Identities = 20/84 (23%), Positives = 37/84 (44%) Frame = +2 Query: 407 QLLSYSNVLGDLDIPKVKKQSLQHLKSSVEEDFQFLSELAALKAVTEKVESGAISADNII 586 QLLSYSN + K+++ ++ +++S F L + A +E + + Sbjct: 274 QLLSYSNQVASYQAIKIEEGDIESIENSHPVFFLVLYDFATTSEDFSIIERLKLQLAGVA 333 Query: 587 DFYNLRINSLHALRDFHGPNSLPT 658 Y I + AL + +G S P+ Sbjct: 334 PLY---ICNSKALANKYGAQSQPS 354 >SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schizosaccharomyces pombe|chr 2|||Manual Length = 809 Score = 25.8 bits (54), Expect = 6.0 Identities = 14/43 (32%), Positives = 25/43 (58%), Gaps = 1/43 (2%) Frame = +2 Query: 50 GELSILHSPESLSFSGSSK-TFESLLKEIFSASLGLSVEENSE 175 G + + SP +++ SGS + E++ EIF + GLS + +E Sbjct: 132 GMIVMGESPMNMAKSGSKNLSSENMATEIFEINSGLSAQSGTE 174 >SPAC343.09 |ubx3|mug39|UBX domain protein Ubx3|Schizosaccharomyces pombe|chr 1|||Manual Length = 410 Score = 25.8 bits (54), Expect = 6.0 Identities = 14/35 (40%), Positives = 20/35 (57%) Frame = +2 Query: 293 LVVDEYEPDTFDVLKHRINQRFTNGGNKLVNINLS 397 L +DE +P T RI R +NGG ++ +NLS Sbjct: 330 LQIDENKPTT------RIQVRLSNGGRTVLTVNLS 358 >SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 1|||Manual Length = 905 Score = 25.8 bits (54), Expect = 6.0 Identities = 22/78 (28%), Positives = 36/78 (46%), Gaps = 1/78 (1%) Frame = +2 Query: 299 VDEY-EPDTFDVLKHRINQRFTNGGNKLVNINLSDSDQLLSYSNVLGDLDIPKVKKQSLQ 475 V E+ + ++FD ++ F N +N S + LLSY+ VLG ++ L Sbjct: 364 VSEFVDENSFDSSSSCSSKVFLTTRNNSINSEDSAHEVLLSYNRVLGS----DIQGTILD 419 Query: 476 HLKSSVEEDFQFLSELAA 529 +K + D Q SEL + Sbjct: 420 RVKKGYQFDSQKNSELVS 437 >SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccharomyces pombe|chr 1|||Manual Length = 569 Score = 25.4 bits (53), Expect = 7.9 Identities = 28/105 (26%), Positives = 42/105 (40%), Gaps = 1/105 (0%) Frame = +2 Query: 41 NASGELSILHSPESLSFSGSSKTFESLLKEIFSASLGLSVEENSEWNGL-LITDPFNTPE 217 ++SG+ S L +LSFS S T E ++ + +S S N L L T P Sbjct: 466 SSSGDFSALSCHRNLSFSESLPTAED---QVHNNHCAVSPSRRSSSNALYLATLPMGYER 522 Query: 218 AVVEVYISGISSLGSSADFKSKKYPLVVDEYEPDTFDVLKHRINQ 352 + Y S S D + P ++ FDVL + + Q Sbjct: 523 SNSVSYCSDSSLSSPLPDDNMLQDPHALNMAYAKEFDVLINEVLQ 567 >SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosaccharomyces pombe|chr 2|||Manual Length = 2335 Score = 25.4 bits (53), Expect = 7.9 Identities = 9/16 (56%), Positives = 12/16 (75%) Frame = +3 Query: 651 YQLRKLKSYWVKALEE 698 Y LRK +SYW K L++ Sbjct: 1875 YDLRKARSYWQKFLQD 1890 >SPCC777.04 |||amino acid transporter |Schizosaccharomyces pombe|chr 3|||Manual Length = 521 Score = 25.4 bits (53), Expect = 7.9 Identities = 19/57 (33%), Positives = 29/57 (50%), Gaps = 1/57 (1%) Frame = +2 Query: 38 INASGELSILHSPESLSFSGSSKTFESLLKEIFSAS-LGLSVEENSEWNGLLITDPF 205 INA + I+ S S + GS +T +L KE ++ L L ++ W G L+T F Sbjct: 337 INACLLVFIISSANSDIYIGS-RTLYALAKEGYAPKILMLQTKQGIPWVGCLVTSSF 392 >SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 25.4 bits (53), Expect = 7.9 Identities = 15/41 (36%), Positives = 24/41 (58%), Gaps = 1/41 (2%) Frame = +2 Query: 59 SILHSPE-SLSFSGSSKTFESLLKEIFSASLGLSVEENSEW 178 S + SPE +L S S+ TF+SL KE+ L+ ++ E+ Sbjct: 93 SNVSSPEKTLLTSASTSTFDSLKKELLPELPSLAYSDDDEF 133 >SPCC5E4.03c |taf72||transcription factor TFIID complex subunit 5 Taf72|Schizosaccharomyces pombe|chr 3|||Manual Length = 643 Score = 25.4 bits (53), Expect = 7.9 Identities = 11/43 (25%), Positives = 24/43 (55%) Frame = +2 Query: 260 SSADFKSKKYPLVVDEYEPDTFDVLKHRINQRFTNGGNKLVNI 388 ++ ++ KY L + TFD+L H + + +NGG+ ++ + Sbjct: 139 TAQQYRQNKYQL---HFSRITFDLLLHFLFENVSNGGSIIIKL 178 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,769,292 Number of Sequences: 5004 Number of extensions: 56339 Number of successful extensions: 242 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 230 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 242 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 325165428 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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