BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= maV30176 (726 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 340 1e-94 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 193 2e-50 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 192 6e-50 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 174 9e-45 SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosacc... 30 0.39 SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 29 0.89 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 2.1 SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 2.7 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 2.7 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 3.6 SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pomb... 26 4.8 SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccha... 26 4.8 SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence orphan|Schizos... 26 6.3 SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 8.3 SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb... 25 8.3 SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 8.3 SPAC32A11.03c |phx1||homeobox transcription factor Phx1|Schizosa... 25 8.3 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 340 bits (836), Expect = 1e-94 Identities = 152/222 (68%), Positives = 179/222 (80%) Frame = +3 Query: 60 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 239 MREIVH+QAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60 Query: 240 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 419 PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120 Query: 420 RKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDTVV 599 R+E+E+CD LQGFQ KIREEYPDR+M T+SV P+PK SDTVV Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVV 180 Query: 600 EPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTY 725 EPYNATLS+HQLVEN+DET+CIDNEAL I TLK+ +P+Y Sbjct: 181 EPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSY 222 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 193 bits (471), Expect = 2e-50 Identities = 91/228 (39%), Positives = 134/228 (58%), Gaps = 6/228 (2%) Frame = +3 Query: 60 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 221 MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60 Query: 222 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 401 GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120 Query: 402 SVLDVVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPK 581 SVL+ +R+ +++C LQGF ++ EY + +SV P+P+ Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQ 180 Query: 582 VSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTY 725 VS +VVEPYN+ L+ H ++N+D T+ +DNEA YDIC R L + PTY Sbjct: 181 VSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTY 228 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 192 bits (467), Expect = 6e-50 Identities = 90/224 (40%), Positives = 131/224 (58%), Gaps = 2/224 (0%) Frame = +3 Query: 60 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 233 MREI+ + GQ G QIG WE+ EHGI P G + ++ Q + +++E GK Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 234 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 413 YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120 Query: 414 VVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDT 593 +R+ +++C LQGF ++ EY + +SV P+P+VS + Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180 Query: 594 VVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTY 725 VVEPYN+ L+ H ++ D T+ +DNE+ YDIC R L + P+Y Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSY 224 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 174 bits (424), Expect = 9e-45 Identities = 82/224 (36%), Positives = 132/224 (58%), Gaps = 3/224 (1%) Frame = +3 Query: 63 REIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 242 REI+ LQAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62 Query: 243 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 416 RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+ Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121 Query: 417 VRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVP-SPKVSDT 593 + +E++ D L+GF ++ + YP +I+ TYSV P S VSD Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDV 181 Query: 594 VVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTY 725 VV+PYN+ L++ +L N D +DN AL I L PT+ Sbjct: 182 VVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTF 225 >SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosaccharomyces pombe|chr 2|||Manual Length = 658 Score = 29.9 bits (64), Expect = 0.39 Identities = 29/105 (27%), Positives = 50/105 (47%) Frame = +1 Query: 364 PRDTTQRVLSSLTRSSM*SAKNQNLAIAYRASNLHIPSVAAPGPVWAPSSSQRSVKSTPT 543 PR++T++ L S++ S S+ Q + +S L+ PS AP P + SSS T Sbjct: 219 PRESTEKPLLSVSALSS-SSHLQPTSATSSSSRLY-PSRPAPTPPASSSSSPLLSSQTVK 276 Query: 544 ES*THTQ*SPRPKYQTLSSNHTMRLSQFTS*LKTQTKPTASTTRL 678 + ++ P P + S+++ +R S L QT T+ T + Sbjct: 277 TTTSNASRQPSPLVSSKSTDNIIR-SHSPVLLTPQTLSTSETKHI 320 >SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 28.7 bits (61), Expect = 0.89 Identities = 24/102 (23%), Positives = 53/102 (51%) Frame = +1 Query: 391 SSLTRSSM*SAKNQNLAIAYRASNLHIPSVAAPGPVWAPSSSQRSVKSTPTES*THTQ*S 570 SS T ++ S+ + + + + +S+ S ++ + SSS S S+ + S + + S Sbjct: 147 SSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS 206 Query: 571 PRPKYQTLSSNHTMRLSQFTS*LKTQTKPTASTTRLYTTSAS 696 P + SS+H+ S +S + ++P++S++ + T S+S Sbjct: 207 SVPITSSTSSSHSSSSSSSSS-SSSSSRPSSSSSFITTMSSS 247 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 27.5 bits (58), Expect = 2.1 Identities = 13/30 (43%), Positives = 19/30 (63%) Frame = +3 Query: 363 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 452 A+GH G ELV + D +RK+SE+ L+ Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212 >SPBC19G7.16 |iws1||transcription elongation factor complex subunit Iws1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 27.1 bits (57), Expect = 2.7 Identities = 10/34 (29%), Positives = 22/34 (64%) Frame = +3 Query: 99 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 200 N++G E+++++ +DPT A + DLQ++ + Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 27.1 bits (57), Expect = 2.7 Identities = 28/91 (30%), Positives = 42/91 (46%) Frame = +1 Query: 454 ASNLHIPSVAAPGPVWAPSSSQRSVKSTPTES*THTQ*SPRPKYQTLSSNHTMRLSQFTS 633 +S+L P + P + SS+ + T T S T T SP P T + T + +S Sbjct: 184 SSSLSTP-ITPTVPPTSTSSTSIPIPPTSTSS-TDTNSSPLPTTSTSCTTSTSIPTGGSS 241 Query: 634 *LKTQTKPTASTTRLYTTSASALSNCPHPPT 726 L T PT T +TS++++ P PPT Sbjct: 242 SLSTPITPTVPPT---STSSTSI---PIPPT 266 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 26.6 bits (56), Expect = 3.6 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +3 Query: 294 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 395 GP+G +F P F+F +G NW+ Y E A L Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190 >SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pombe|chr 1|||Manual Length = 258 Score = 26.2 bits (55), Expect = 4.8 Identities = 11/32 (34%), Positives = 19/32 (59%) Frame = +1 Query: 436 LAIAYRASNLHIPSVAAPGPVWAPSSSQRSVK 531 LA+ Y + + +V APG +W+P+ +R K Sbjct: 159 LAVRYGPLGIRV-NVCAPGTIWSPAWDERFKK 189 >SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 26.2 bits (55), Expect = 4.8 Identities = 11/19 (57%), Positives = 15/19 (78%) Frame = +1 Query: 43 KITYTK*GKSFIYRPANVV 99 +I+YT G SFIYRP N++ Sbjct: 64 EISYT--GTSFIYRPGNII 80 >SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 1563 Score = 25.8 bits (54), Expect = 6.3 Identities = 11/28 (39%), Positives = 15/28 (53%) Frame = +1 Query: 643 TQTKPTASTTRLYTTSASALSNCPHPPT 726 T T +T + TTS S ++CP P T Sbjct: 166 TSTSCNPATVLIVTTSGSTSTSCPPPTT 193 >SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1063 Score = 25.4 bits (53), Expect = 8.3 Identities = 12/44 (27%), Positives = 20/44 (45%) Frame = +3 Query: 315 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 446 RP +F G++ G + E D ++ + + ESCDC Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997 >SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 262 Score = 25.4 bits (53), Expect = 8.3 Identities = 22/78 (28%), Positives = 34/78 (43%) Frame = +1 Query: 475 SVAAPGPVWAPSSSQRSVKSTPTES*THTQ*SPRPKYQTLSSNHTMRLSQFTS*LKTQTK 654 S A P SSS S STP S + S SS+ + S ++ KT + Sbjct: 145 STATPSSSSTTSSSSSSSSSTPISSSITSSISSSASSSVSSSSASSSGSISSADAKTVSA 204 Query: 655 PTASTTRLYTTSASALSN 708 + ST ++TS ++ S+ Sbjct: 205 SSNSTISGFSTSTTSASS 222 >SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 25.4 bits (53), Expect = 8.3 Identities = 13/32 (40%), Positives = 16/32 (50%) Frame = -3 Query: 352 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 257 P+ PK L R I P GPE + + GS S Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51 >SPAC32A11.03c |phx1||homeobox transcription factor Phx1|Schizosaccharomyces pombe|chr 1|||Manual Length = 942 Score = 25.4 bits (53), Expect = 8.3 Identities = 15/47 (31%), Positives = 20/47 (42%) Frame = +1 Query: 577 PKYQTLSSNHTMRLSQFTS*LKTQTKPTASTTRLYTTSASALSNCPH 717 PK T H +S T +K+ S+TRL + S S PH Sbjct: 664 PKTNTEGLMHAQSVSPITQAMKSANVLEGSSTRLNSYEPSVSSAYPH 710 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,827,699 Number of Sequences: 5004 Number of extensions: 57178 Number of successful extensions: 199 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 184 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 191 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 341222980 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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