Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30165
(660 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g59900.1 68414.m06748 pyruvate dehydrogenase E1 component alp... 141 4e-34
At1g24180.1 68414.m03050 pyruvate dehydrogenase E1 component alp... 140 1e-33
At1g01090.1 68414.m00011 pyruvate dehydrogenase E1 component alp... 101 4e-22
At4g18670.1 68417.m02762 leucine-rich repeat family protein / ex... 31 0.68
At5g14920.1 68418.m01750 gibberellin-regulated family protein si... 29 2.1
At4g16980.1 68417.m02560 arabinogalactan-protein family similar ... 29 2.1
At1g25540.1 68414.m03171 phytochrome and flowering time regulato... 29 2.7
At4g12470.1 68417.m01972 protease inhibitor/seed storage/lipid t... 29 3.6
At5g56330.1 68418.m07031 carbonic anhydrase family protein conta... 28 4.8
At3g24550.1 68416.m03083 protein kinase family protein contains ... 28 4.8
At5g57710.1 68418.m07214 heat shock protein-related contains sim... 28 6.3
At3g44210.1 68416.m04741 hypothetical protein 28 6.3
At3g19840.1 68416.m02513 FF domain-containing protein / WW domai... 28 6.3
At4g15740.1 68417.m02396 C2 domain-containing protein similar to... 27 8.4
At3g63500.2 68416.m07153 expressed protein 27 8.4
At3g63500.1 68416.m07152 expressed protein 27 8.4
>At1g59900.1 68414.m06748 pyruvate dehydrogenase E1 component alpha
subunit, mitochondrial (PDHE1-A) identical to SP|P52901
Pyruvate dehydrogenase E1 component alpha subunit,
mitochondrial precursor (EC 1.2.4.1) (PDHE1-A)
{Arabidopsis thaliana}
Length = 389
Score = 141 bits (341), Expect = 4e-34
Identities = 69/133 (51%), Positives = 92/133 (69%), Gaps = 2/133 (1%)
Frame = +2
Query: 266 PYKLHKLDQGPATSATLTSEDALKLYEQLTILRRIETASGNLYKEKIIRGFCHLYSGQEA 445
P+ H D P+ S +S++ L + + ++RR+E A+ +LYK K+IRGFCHLY GQEA
Sbjct: 39 PFTAHLCDP-PSRSVESSSQELLDFFRTMALMRRMEIAADSLYKAKLIRGFCHLYDGQEA 97
Query: 446 VAVGMRAAMRDADSVITAYRCHGWTYLMGVSVLGVLSELTGRRTGCSRGKGGSMHLYGR- 622
VA+GM AA+ D++ITAYR H G S+ V SEL GR+ GCS+GKGGSMH Y +
Sbjct: 98 VAIGMEAAITKKDAIITAYRDHCIFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHFYKKE 157
Query: 623 -NFYGGNGIVGAQ 658
+FYGG+GIVGAQ
Sbjct: 158 SSFYGGHGIVGAQ 170
>At1g24180.1 68414.m03050 pyruvate dehydrogenase E1 component alpha
subunit, mitochondrial, putative similar to SP|P52901
Pyruvate dehydrogenase E1 component alpha subunit,
mitochondrial precursor (EC 1.2.4.1) (PDHE1-A)
{Arabidopsis thaliana}; contains Pfam profile PF00676:
Dehydrogenase E1 component
Length = 393
Score = 140 bits (338), Expect = 1e-33
Identities = 68/133 (51%), Positives = 91/133 (68%), Gaps = 2/133 (1%)
Frame = +2
Query: 266 PYKLHKLDQGPATSATLTSEDALKLYEQLTILRRIETASGNLYKEKIIRGFCHLYSGQEA 445
P+ H L + P+ S +SE+ L + + +RR+E A+ +LYK K+IRGFCHLY GQEA
Sbjct: 43 PFTSH-LCESPSRSVETSSEEILAFFRDMARMRRMEIAADSLYKAKLIRGFCHLYDGQEA 101
Query: 446 VAVGMRAAMRDADSVITAYRCHGWTYLMGVSVLGVLSELTGRRTGCSRGKGGSMHLYGR- 622
+AVGM AA+ D++IT+YR H G ++ SEL GR+TGCS GKGGSMH Y +
Sbjct: 102 LAVGMEAAITKKDAIITSYRDHCTFIGRGGKLVDAFSELMGRKTGCSHGKGGSMHFYKKD 161
Query: 623 -NFYGGNGIVGAQ 658
+FYGG+GIVGAQ
Sbjct: 162 ASFYGGHGIVGAQ 174
>At1g01090.1 68414.m00011 pyruvate dehydrogenase E1 component alpha
subunit, chloroplast identical to pyruvate dehydrogenase
E1 alpha subunit GB:AAB86803 GI:2454182 from
[Arabidopsis thaliana]; identical to cDNA pyruvate
dehydrogenase E1 alpha subunit mRNA, nuclear gene
encoding plastid protein GI:2454181
Length = 428
Score = 101 bits (242), Expect = 4e-22
Identities = 50/119 (42%), Positives = 70/119 (58%), Gaps = 2/119 (1%)
Frame = +2
Query: 302 TSATLTSEDALKLYEQLTILRRIETASGNLYKEKIIRGFCHLYSGQEAVAVGMRAAMRDA 481
TS +T E+ L+LYE + + R E +Y + GF HLY+GQEAV+ G + +
Sbjct: 75 TSLLITKEEGLELYEDMILGRSFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKS 134
Query: 482 DSVITAYRCHGWTYLMGVSVLGVLSELTGRRTGCSRGKGGSMHLYGR--NFYGGNGIVG 652
DSV++ YR H GVS V+SEL G+ TGC RG+GGSMH++ + N GG +G
Sbjct: 135 DSVVSTYRDHVHALSKGVSARAVMSELFGKVTGCCRGQGGSMHMFSKEHNMLGGFAFIG 193
>At4g18670.1 68417.m02762 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 839
Score = 31.1 bits (67), Expect = 0.68
Identities = 20/51 (39%), Positives = 23/51 (45%), Gaps = 4/51 (7%)
Frame = -1
Query: 651 PTIPLPP*K-LRPYKCMEPPLPR---EQPVLRPVSSESTPSTLTPMR*VHP 511
P IP PP P PPLP P++ P S PST TP +HP
Sbjct: 579 PIIPSPPFTGPSPPSSPSPPLPPVIPSPPIVGPTPSSPPPSTPTPGTLLHP 629
>At5g14920.1 68418.m01750 gibberellin-regulated family protein
similar to SP|P46689 Gibberellin-regulated protein 1
precursor {Arabidopsis thaliana}; contains Pfam profile
PF02704: Gibberellin regulated protein
Length = 275
Score = 29.5 bits (63), Expect = 2.1
Identities = 14/44 (31%), Positives = 22/44 (50%), Gaps = 1/44 (2%)
Frame = -1
Query: 651 PTIPLP-P*KLRPYKCMEPPLPREQPVLRPVSSESTPSTLTPMR 523
PT+P P P P ++PP P +P P + P+T P++
Sbjct: 36 PTLPSPSPATKPPSPALKPPTPSYKPPTLPTTPIKPPTTKPPVK 79
>At4g16980.1 68417.m02560 arabinogalactan-protein family similar to
arabinogalactan protein [Arabidopsis thaliana]
gi|10880495|gb|AAG24277; contains proline-rich extensin
domains, INTERPRO:IPR002965
Length = 164
Score = 29.5 bits (63), Expect = 2.1
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = -1
Query: 651 PTIPLPP*KLRPYKCMEPPLPREQPVLRPVSSESTPSTLT 532
P +P+ P P PP+P P + P++ ++PS LT
Sbjct: 70 PPMPMTP---PPMPMAPPPMPMASPPMMPMTPSTSPSPLT 106
>At1g25540.1 68414.m03171 phytochrome and flowering time regulatory
protein (PFT1) PMID: 12815435
Length = 836
Score = 29.1 bits (62), Expect = 2.7
Identities = 23/78 (29%), Positives = 35/78 (44%), Gaps = 4/78 (5%)
Frame = -1
Query: 660 TCAPTIPLPP*KLRPYKCMEPPLPREQPV-LRPVSSEST---PSTLTPMR*VHP*QRYAV 493
T AP+IP+ P P+ QPV + PV + + PST+T M V
Sbjct: 255 TVAPSIPVTGQPPAPVSSANGPIQNRQPVSVGPVPTATVKVEPSTVTSMAPVPSFPHIPA 314
Query: 492 ITESASRIAALIPTATAS 439
+ A++ I T++AS
Sbjct: 315 VARPATQAIPSIQTSSAS 332
>At4g12470.1 68417.m01972 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein similar to pEARLI
1 (Accession No. L43080): an Arabidopsis member of a
conserved gene family (PGF95-099), Plant Physiol. 109
(4), 1497 (1995); contains Pfam protease inhibitor/seed
storage/LTP family domain PF00234
Length = 161
Score = 28.7 bits (61), Expect = 3.6
Identities = 14/36 (38%), Positives = 19/36 (52%), Gaps = 1/36 (2%)
Frame = -1
Query: 642 PLPP*KLRPYKCMEPPLPR-EQPVLRPVSSESTPST 538
P+P K +P +C PP P P RPV+ TP +
Sbjct: 38 PVPSPKPKPVQCPPPPRPSVPSPNPRPVTPPRTPGS 73
>At5g56330.1 68418.m07031 carbonic anhydrase family protein contains
proline-rich extensin domains, INTERPRO:IPR002965;
contains Pfam profile PF00194: Eukaryotic-type carbonic
anhydrase
Length = 350
Score = 28.3 bits (60), Expect = 4.8
Identities = 13/38 (34%), Positives = 19/38 (50%)
Frame = -1
Query: 642 PLPP*KLRPYKCMEPPLPREQPVLRPVSSESTPSTLTP 529
P PP K +P PP P+ +P P + + TP+ P
Sbjct: 70 PTPP-KPKPAPAPTPPKPKPKPAPTPPNPKPTPAPTPP 106
>At3g24550.1 68416.m03083 protein kinase family protein contains
Pfam domain PF00069: Protein kinase domain
Length = 652
Score = 28.3 bits (60), Expect = 4.8
Identities = 16/43 (37%), Positives = 18/43 (41%), Gaps = 1/43 (2%)
Frame = -1
Query: 654 APTIPLPP*KLRPYKCMEPPLPREQPVLRPV-SSESTPSTLTP 529
AP P PP P PP P + P P S+ TPS P
Sbjct: 84 APITPSPPSPTTPSNPRSPPSPNQGPPNTPSGSTPRTPSNTKP 126
>At5g57710.1 68418.m07214 heat shock protein-related contains
similarity to 101 kDa heat shock protein; HSP101
[Triticum aestivum] gi|11561808|gb|AAC83689
Length = 990
Score = 27.9 bits (59), Expect = 6.3
Identities = 15/49 (30%), Positives = 21/49 (42%), Gaps = 1/49 (2%)
Frame = -3
Query: 649 NNPVATVEVASVQMHGTSLAPGAAGPAPRQLREHPQ-HTNAHEISPSVT 506
NN V + SV G + PG GP R +P+ NA + V+
Sbjct: 166 NNSVTPTPIPSVSSVGLNFRPGGGGPMTRNSYLNPRLQQNASSVQSGVS 214
>At3g44210.1 68416.m04741 hypothetical protein
Length = 143
Score = 27.9 bits (59), Expect = 6.3
Identities = 11/33 (33%), Positives = 21/33 (63%)
Frame = +2
Query: 470 MRDADSVITAYRCHGWTYLMGVSVLGVLSELTG 568
+R ADS+ ++ H + L+G+ + GV+ + TG
Sbjct: 9 LRVADSLSIGFKLHPYLTLIGMKLSGVIHDTTG 41
>At3g19840.1 68416.m02513 FF domain-containing protein / WW
domain-containing protein weak similarity to
transcription factor CA150b [Mus musculus] GI:6329166;
contains Pfam profiles PF01846: FF domain, PF00397: WW
domain
Length = 743
Score = 27.9 bits (59), Expect = 6.3
Identities = 18/64 (28%), Positives = 32/64 (50%), Gaps = 6/64 (9%)
Frame = +2
Query: 152 IPSAAKFLAGNTITKVTAPVVATNAKYSTKKEAT------FEIKPYKLHKLDQGPATSAT 313
+PSA G+ +T ++AP ++ + + + T ++ KLH D G S+T
Sbjct: 255 VPSADLTEKGSDLTSLSAPAISNGGRDAASLKTTNFGSSALDLVKKKLH--DSGMPVSST 312
Query: 314 LTSE 325
+TSE
Sbjct: 313 ITSE 316
>At4g15740.1 68417.m02396 C2 domain-containing protein similar to
cold-regulated gene SRC2 [Glycine max] GI:2055230;
contains Pfam profile PF00168: C2 domain
Length = 468
Score = 27.5 bits (58), Expect = 8.4
Identities = 27/122 (22%), Positives = 50/122 (40%), Gaps = 8/122 (6%)
Frame = +2
Query: 71 LLSVVLQKDH*INLGFIEIIIDKMSKLIPSAAKFLAGNT--ITKVTAPVVATNAKYSTKK 244
L S L+ D+ + LG + + + + P F GN + +T P+ AT +T
Sbjct: 117 LFSYWLEGDNDLYLGEVNVSVQDLLASNP-IPPFTNGNINKMKSMTCPIKATEKSTNTTV 175
Query: 245 EATFEIKPYKLHKL-----DQGPATSATLTSE-DALKLYEQLTILRRIETASGNLYKEKI 406
++ KP + + D P+ + + D KL + L R + + L E +
Sbjct: 176 SLSYRFKPVPVEEFYPPSPDSLPSIGKPVYRDLDPAKLSQPLVFSPRFQATTTKLILELV 235
Query: 407 IR 412
I+
Sbjct: 236 IK 237
>At3g63500.2 68416.m07153 expressed protein
Length = 1162
Score = 27.5 bits (58), Expect = 8.4
Identities = 11/29 (37%), Positives = 17/29 (58%), Gaps = 1/29 (3%)
Frame = +3
Query: 243 KRRHSKSSPTNSINWIRAL-QHQPHSLLK 326
K RH + SP+ W+R++ PH+ LK
Sbjct: 994 KPRHERGSPSQDTAWLRSVCSDNPHNQLK 1022
>At3g63500.1 68416.m07152 expressed protein
Length = 887
Score = 27.5 bits (58), Expect = 8.4
Identities = 11/29 (37%), Positives = 17/29 (58%), Gaps = 1/29 (3%)
Frame = +3
Query: 243 KRRHSKSSPTNSINWIRAL-QHQPHSLLK 326
K RH + SP+ W+R++ PH+ LK
Sbjct: 719 KPRHERGSPSQDTAWLRSVCSDNPHNQLK 747
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,932,737
Number of Sequences: 28952
Number of extensions: 277539
Number of successful extensions: 869
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 816
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 866
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1383534864
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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