SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= maV30160
         (709 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC16E8.07c |vph1||V-type ATPase subunit a|Schizosaccharomyces ...   101   8e-23
SPBC3D6.04c |mad1||mitotic spindle checkpoint protein Mad1|Schiz...    31   0.12 
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch...    31   0.16 
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce...    31   0.21 
SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomy...    30   0.37 
SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosacchar...    29   0.86 
SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase |Schizo...    29   0.86 
SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|...    27   2.0  
SPBC1711.09c |||SNARE associated Golgi protein |Schizosaccharomy...    27   2.0  
SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schi...    27   2.6  
SPAC22F3.13 |tsc1||hamartin|Schizosaccharomyces pombe|chr 1|||Ma...    27   3.5  
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M...    27   3.5  
SPCC297.03 |ssp1||serine/threonine protein kinase Ssp1 |Schizosa...    26   4.6  
SPCC553.04 |cyp9||WD repeat containing cyclophilin family peptid...    26   4.6  
SPBC23E6.09 |ssn6||transcriptional corepressor Ssn6|Schizosaccha...    26   6.1  
SPAC1006.05c |och1||alpha-1,6-mannosyltransferase Och1 |Schizosa...    25   8.0  

>SPAC16E8.07c |vph1||V-type ATPase subunit a|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 805

 Score =  101 bits (243), Expect = 8e-23
 Identities = 71/213 (33%), Positives = 105/213 (49%), Gaps = 31/213 (14%)
 Frame = +2

Query: 164 VSELGEAGSVQFRDLNPDVNAFQRKFVNEVRRCDEMERKLRYIEAEVHKDGVHIPAVKEA 343
           +S LGE  ++ F+DLNPDV AFQR FV E+RR  + ER LRY+ +E+  +G+H+P     
Sbjct: 1   MSALGELSTIHFKDLNPDVVAFQRSFVREIRRLTDTERLLRYLHSEIDLNGIHVPDHNLP 60

Query: 344 PRAPNPRE---IIDLEAHLEKTENEILELSHNAVNLKQNYLELTELRHVLEKTEAFF--- 505
           P   +  E   I D+   + + E  + +L  ++  L+  YL+  E  +VL K +AFF   
Sbjct: 61  PSYESVLESSTIEDIIERITRLEARVRQLVESSQLLEARYLQQLEFANVLTKADAFFSKS 120

Query: 506 ------------TAQEEIGMDSLTKSLI-------------SDETGQQAATRGRLGFVAG 610
                       T+    G D  T  LI             S+ET  Q  T   L FV+G
Sbjct: 121 GNTVDPLRNNYETSSIFSGEDDTTAPLIENALELGTTGTFDSEETSPQMNT--TLDFVSG 178

Query: 611 VVQRERVPAFERMLWRISRGNVFLRRAELDKPL 709
           ++   +    ER+LWR  RGN+F+ +   D  L
Sbjct: 179 IIPTVKFQFLERILWRTLRGNLFIHQVRADDSL 211


>SPBC3D6.04c |mad1||mitotic spindle checkpoint protein
           Mad1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 689

 Score = 31.5 bits (68), Expect = 0.12
 Identities = 26/108 (24%), Positives = 47/108 (43%), Gaps = 4/108 (3%)
 Frame = +2

Query: 284 RYIEAEVHKDGVHIPAVKEAPRAPNPREIIDLEAHLEKTENEI----LELSHNAVNLKQN 451
           R++   V K  +  P+V  A    NP+ +  LE  LE  +N++    LE     + L++ 
Sbjct: 30  RFLATSVKKPNLKKPSVNSANETKNPK-LASLEFQLENLKNDLKRKELEFEREQIELQRK 88

Query: 452 YLELTELRHVLEKTEAFFTAQEEIGMDSLTKSLISDETGQQAATRGRL 595
             E  E ++ L+        Q E    S  K  I +   ++ AT+ ++
Sbjct: 89  LAEEHEQKNSLQLRLTLVEKQLEEQSTSYQKE-IEEVRNEKEATQVKI 135


>SPCC162.08c |nup211||nuclear pore complex associated
            protein|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1837

 Score = 31.1 bits (67), Expect = 0.16
 Identities = 22/103 (21%), Positives = 50/103 (48%), Gaps = 2/103 (1%)
 Frame = +2

Query: 203  DLNPDVNAFQRKFVNEVR-RCDEMERKLRYIEA-EVHKDGVHIPAVKEAPRAPNPREIID 376
            +L  D+   QR   N++  +  E++ +L+++E  + +     I A K+  + P+  E+  
Sbjct: 889  ELKQDIT-LQRTVRNQLEIQTTELKERLKFMEERQENLQSKLIAANKDTTQNPDNVEVEA 947

Query: 377  LEAHLEKTENEILELSHNAVNLKQNYLELTELRHVLEKTEAFF 505
            +   LE+T+ ++        N++Q YL   +   ++ +T   F
Sbjct: 948  ISIELERTKEKLRMAELEKSNIQQKYLASEKTLEMMNETHEQF 990


>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1036

 Score = 30.7 bits (66), Expect = 0.21
 Identities = 50/188 (26%), Positives = 76/188 (40%), Gaps = 8/188 (4%)
 Frame = -2

Query: 708 SGLSSSARRRKTLPLEIRHSIRSNAG-----TRSRWTTPATNPRRPRVAAC*PVSSDIKD 544
           S L+SS+    T       SI S        T S  TT AT+      +A    SS +  
Sbjct: 225 SSLASSSLNSTTSATATSSSISSTVSSSTPLTSSNSTTAATSASATSSSAQYNTSSLLPS 284

Query: 543 LVKESMPISSCAVKKASVFSRTCLNSVNS-R*FCFKLTALCDSSRISFSVFSKCASKSMI 367
               S P+SS     A+  S T L SVNS        T L   S  + +  +  +S  + 
Sbjct: 285 STPSSTPLSSANSTTATSASSTPLTSVNSTTTTSASSTPLSSVSSANSTTATSTSSTPLS 344

Query: 366 SL-GLGARGASLTAGMWTPSLCTSASMYRSLRSISSQRRTSLTNF-LWKAFTSGFKSLNC 193
           S+    A  AS T      S   +++    L S++S   TS ++  L  A ++   S++ 
Sbjct: 345 SVNSTTATSASSTPLTSVNSTTATSASSTPLTSVNSTSATSASSTPLTSANSTTSTSVSS 404

Query: 192 TLPASPSS 169
           T P+  +S
Sbjct: 405 TAPSYNTS 412



 Score = 27.9 bits (59), Expect = 1.5
 Identities = 26/92 (28%), Positives = 42/92 (45%), Gaps = 3/92 (3%)
 Frame = -2

Query: 435 TALCDSSRISFSVFSKCASKSMISLGL---GARGASLTAGMWTPSLCTSASMYRSLRSIS 265
           ++L  SS ++ S  +   S S  S  L    A  +SL +   T S   S+S+  S  + S
Sbjct: 83  SSLTSSSSLASSSTNSTTSASPTSSSLTSSSATSSSLASSSTTSSSLASSSITSSSLASS 142

Query: 264 SQRRTSLTNFLWKAFTSGFKSLNCTLPASPSS 169
           S   +SL +    + +    S N T  A+P+S
Sbjct: 143 SITSSSLASSSTTSSSLASSSTNSTTSATPTS 174



 Score = 27.9 bits (59), Expect = 1.5
 Identities = 52/192 (27%), Positives = 80/192 (41%), Gaps = 7/192 (3%)
 Frame = -2

Query: 699 SSSARRRKTLPLEIRHSIRSNAGTRSRWT--TPATNPRRPRVAAC*P---VSSDIKDLVK 535
           S++A    + PL    S+ S + T +  T  T A +     V++  P    SS +     
Sbjct: 364 STTATSASSTPLT---SVNSTSATSASSTPLTSANSTTSTSVSSTAPSYNTSSVLPTSSV 420

Query: 534 ESMPISSCAVKKASVFSRTCLNSVNSR*FCFKLTALCDSSRISFSVFSKCASKSMISLGL 355
            S P+SS     A+  S T L+SVNS       TA   SS    SV S  A+ +  +   
Sbjct: 421 SSTPLSSANSTTATSASSTPLSSVNS------TTATSASSTPLSSVNSTTATSASSTPLT 474

Query: 354 GARGASLTAGMWTPSLCTSASMYRSLRSISSQRRTSLTNFLWKAFTSGFKSLNCT--LPA 181
                + T+   TP    +++   S  S SS   TS  +    + +S   S N +  LP 
Sbjct: 475 SVNSTTATSASSTPLTSVNST---SATSASSTPLTSANSTTSTSVSSTAPSYNTSSVLPT 531

Query: 180 SPSSETEV*AAS 145
           S  S T + +A+
Sbjct: 532 SSVSSTPLSSAN 543


>SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 1115

 Score = 29.9 bits (64), Expect = 0.37
 Identities = 19/57 (33%), Positives = 32/57 (56%), Gaps = 1/57 (1%)
 Frame = +2

Query: 152  AYTSVSELGEAGSVQFRDLNPDVNAFQRKFVNEVRRCDEMERKLRYIEAEVHK-DGV 319
            A T++S + +A ++ FR+L+  +  F+RK       C  MER+ +    E+ K DGV
Sbjct: 934  ALTTISSMNQAVNMNFRELDKSIQEFKRK-------CQSMEREFK---TELRKLDGV 980


>SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3
            |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1131

 Score = 28.7 bits (61), Expect = 0.86
 Identities = 23/84 (27%), Positives = 42/84 (50%), Gaps = 1/84 (1%)
 Frame = -2

Query: 393  SKCASKSMISLGLGARGASLTAGMWTPSLCTSASMYRSLRSISSQRRT-SLTNFLWKAFT 217
            S  +S S  S  + +  +S T      +  +S+++  S+ SI++   T    + L  + T
Sbjct: 931  SLTSSVSTASSYIASSASSNTLSTEPKTFSSSSTLSESISSINTNSLTVKPESSLSSSTT 990

Query: 216  SGFKSLNCTLPASPSSETEV*AAS 145
            SG  S + T+P+S  SE+   +AS
Sbjct: 991  SGLTSSSSTIPSSTRSESNSESAS 1014


>SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 944

 Score = 28.7 bits (61), Expect = 0.86
 Identities = 11/21 (52%), Positives = 16/21 (76%), Gaps = 1/21 (4%)
 Frame = -1

Query: 301 LGLDVPEFAFHFIAT-AHLIN 242
           LG  +PEFA+HF+ T + L+N
Sbjct: 383 LGFQIPEFAYHFLQTCSRLVN 403


>SPBC1826.01c |mot1||TATA-binding protein associated factor
           Mot1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1953

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 12/28 (42%), Positives = 16/28 (57%)
 Frame = -2

Query: 294 SMYRSLRSISSQRRTSLTNFLWKAFTSG 211
           SMY+ L S+  Q    LTN +W+A   G
Sbjct: 467 SMYKVLHSLVFQNELGLTNTVWEAAHGG 494


>SPBC1711.09c |||SNARE associated Golgi protein |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 270

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 14/56 (25%), Positives = 26/56 (46%)
 Frame = +2

Query: 317 VHIPAVKEAPRAPNPREIIDLEAHLEKTENEILELSHNAVNLKQNYLELTELRHVL 484
           V   A+K +    +     D+  HLE    + ++L H     +QN  + +E +H+L
Sbjct: 211 VRYKAIKNSTLEDSTNSTSDVLNHLESQPTDSIDLRHLEQTSEQNETDNSERKHLL 266


>SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 749

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 16/46 (34%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
 Frame = -2

Query: 303 TSASMYRSL-RSISSQRRTSLTNFLWKAFTSGFKSLNCTLPASPSS 169
           +SAS YRS  +  S+   T+ ++F +   TSG  + N   P+S +S
Sbjct: 657 SSASSYRSYSQRASTPAVTAKSSFHYATPTSGLSNFNSVTPSSSAS 702


>SPAC22F3.13 |tsc1||hamartin|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 899

 Score = 26.6 bits (56), Expect = 3.5
 Identities = 17/47 (36%), Positives = 23/47 (48%)
 Frame = +2

Query: 311 DGVHIPAVKEAPRAPNPREIIDLEAHLEKTENEILELSHNAVNLKQN 451
           D   I +  EA      RE+I+ E H  K E+E L L  N   LK++
Sbjct: 725 DSSMILSNSEAVSDEQERELIESEKHRMKLESENLHLQANIELLKKD 771


>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 500

 Score = 26.6 bits (56), Expect = 3.5
 Identities = 18/67 (26%), Positives = 29/67 (43%)
 Frame = +2

Query: 368 IIDLEAHLEKTENEILELSHNAVNLKQNYLELTELRHVLEKTEAFFTAQEEIGMDSLTKS 547
           ++ +E   E + +   E S +    + +  E  E   V+EKTE       E   DS + S
Sbjct: 153 VVKIEEKKESSSDSSSESSSSESESESSSSESEEEEEVVEKTEEKKEGSSESSSDSESSS 212

Query: 548 LISDETG 568
             S E+G
Sbjct: 213 DSSSESG 219


>SPCC297.03 |ssp1||serine/threonine protein kinase Ssp1
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 652

 Score = 26.2 bits (55), Expect = 4.6
 Identities = 16/63 (25%), Positives = 31/63 (49%)
 Frame = +2

Query: 518 EIGMDSLTKSLISDETGQQAATRGRLGFVAGVVQRERVPAFERMLWRISRGNVFLRRAEL 697
           ++G D++T+ L++ +   +   R +LG      Q+E+V     +L +    NV   R  +
Sbjct: 150 KLGRDTVTRELLAIKIIPKTERRPKLGRANASSQKEKVRREIAILKKCVHPNVVRLREVI 209

Query: 698 DKP 706
           D P
Sbjct: 210 DDP 212


>SPCC553.04 |cyp9||WD repeat containing cyclophilin family
           peptidyl-prolyl cis-trans isomerase
           Cyp9|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 610

 Score = 26.2 bits (55), Expect = 4.6
 Identities = 11/37 (29%), Positives = 19/37 (51%)
 Frame = -1

Query: 325 NVDTIFVHLGLDVPEFAFHFIATAHLINEFPLESIYV 215
           N D +F+H   D P +   ++  A +   FP +S Y+
Sbjct: 25  NHDQVFLHNLPDAPRYTKSYMHNAEIYKCFPTKSNYI 61


>SPBC23E6.09 |ssn6||transcriptional corepressor
           Ssn6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1102

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 19/73 (26%), Positives = 36/73 (49%)
 Frame = +2

Query: 461 LTELRHVLEKTEAFFTAQEEIGMDSLTKSLISDETGQQAATRGRLGFVAGVVQRERVPAF 640
           L +L  +  +  + FT Q+ + +  LTKSL +D+T  Q+       +VA     +   A+
Sbjct: 549 LQQLGWLCHQQSSSFTNQD-LAIQYLTKSLEADDTDAQSWYLIGRCYVAQQKYNKAYEAY 607

Query: 641 ERMLWRISRGNVF 679
           ++ ++R  R   F
Sbjct: 608 QQAVYRDGRNPTF 620


>SPAC1006.05c |och1||alpha-1,6-mannosyltransferase Och1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 396

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 17/52 (32%), Positives = 25/52 (48%)
 Frame = +2

Query: 374 DLEAHLEKTENEILELSHNAVNLKQNYLELTELRHVLEKTEAFFTAQEEIGM 529
           DLEA+L   E E LE S   ++ + NY     LR+   + + F    E + M
Sbjct: 76  DLEAYLSDLEREELEHSLEELDEENNY--KLHLRYSFSQLQDFDEENEAVHM 125


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,087,325
Number of Sequences: 5004
Number of extensions: 66412
Number of successful extensions: 236
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 224
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 232
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 329179816
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -