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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= maV30158
         (748 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1006.07 |||translation initiation factor eIF4A|Schizosacchar...    28   1.2  
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha...    27   2.1  
SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces pom...    26   5.0  
SPAPB1A10.09 |ase1||microtubule-associated protein Ase1 |Schizos...    25   8.7  
SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated memb...    25   8.7  
SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    25   8.7  
SPBC3D6.16 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    25   8.7  
SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyce...    25   8.7  
SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual    25   8.7  

>SPAC1006.07 |||translation initiation factor
           eIF4A|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 392

 Score = 28.3 bits (60), Expect = 1.2
 Identities = 9/21 (42%), Positives = 17/21 (80%)
 Frame = +3

Query: 261 AIDLLTNDECRLLLEVEDFFN 323
           +I+ +TND+ R++ E+E F+N
Sbjct: 358 SINFVTNDDVRMMREIEQFYN 378


>SPAC1834.01 |sup45||translation release factor
           eRF1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 433

 Score = 27.5 bits (58), Expect = 2.1
 Identities = 12/30 (40%), Positives = 16/30 (53%)
 Frame = +1

Query: 394 STRHHRRS*NQVPPNSLTIFCSS*TVNANK 483
           STR   +  N+VP N L I+C    +  NK
Sbjct: 74  STRERLKLYNKVPDNGLVIYCGEVIMEGNK 103


>SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 396

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 24/85 (28%), Positives = 36/85 (42%), Gaps = 2/85 (2%)
 Frame = +2

Query: 23  FSCNFAITMGF*YLIVCTPACAFTHN-IKMLAKMAPS-QDKLVHLHKTPTSLDINPSGLL 196
           F C   I++ F   I    A A  H  I  L  + P  +D   H  K      I+P  LL
Sbjct: 310 FVCPSCISLDFDLQI---QAFARQHRVISTLGVVYPEREDSCFHKIKAAQWHQISPRSLL 366

Query: 197 FAFVELNHYNNECESEGVMGCGHRF 271
           F F E NH +++     ++  G ++
Sbjct: 367 FQFQEQNHIHHKKIRRKLLAAGWKW 391


>SPAPB1A10.09 |ase1||microtubule-associated protein Ase1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 731

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
 Frame = +3

Query: 462 LDSQCKQENIFSNWLEEKVD-LPSIFENISEVPERVDPQP 578
           +D  CKQ+ +FS   E++ D L SI   +S +  ++   P
Sbjct: 242 IDQLCKQKEVFSAEKEKRSDHLKSIQSEVSNLWNKLQVSP 281


>SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated
           membrane proteins, ESCRT 0 complex|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 610

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 12/41 (29%), Positives = 20/41 (48%)
 Frame = +3

Query: 504 LEEKVDLPSIFENISEVPERVDPQPPAAVLASSPFVTSQPT 626
           +E + D+ S   N S +P+      P  V+ SSP   + P+
Sbjct: 470 IEPEPDVISTVRNSSTIPQASSSSVPKIVVDSSPVTENPPS 510


>SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 583

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 9/18 (50%), Positives = 12/18 (66%)
 Frame = +3

Query: 279 NDECRLLLEVEDFFNDDC 332
           NDECR L +   FF+ +C
Sbjct: 535 NDECRRLKQCNHFFHREC 552


>SPBC3D6.16 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 95

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 12/29 (41%), Positives = 14/29 (48%)
 Frame = +2

Query: 134 DKLVHLHKTPTSLDINPSGLLFAFVELNH 220
           D + H  K  +S   NPS LLF    L H
Sbjct: 59  DGIPHSRKKVSSAHFNPSTLLFLLKRLGH 87


>SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 2310

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 14/49 (28%), Positives = 23/49 (46%)
 Frame = -3

Query: 404  WRVETRSVIDFDLRCSARKVFKQITIIIEEILNFEQQTALIVSEQIDGR 258
            W   T+ V     R S RKV + + +  EE    +++ ALI  + +  R
Sbjct: 2143 WSTPTKLVEPSQFRASPRKVDQAVVLSSEEKEILQKKYALIAEDNLIAR 2191


>SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 304

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 4/49 (8%)
 Frame = +2

Query: 110 LAKMAPSQDKLVHLH----KTPTSLDINPSGLLFAFVELNHYNNECESE 244
           L +    ++KLV+L      T T LD +   ++ A V+ N Y N C+++
Sbjct: 170 LEEFESQKEKLVYLSADSDNTITELDEDKIYIIGAIVDKNRYKNLCQNK 218


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,816,031
Number of Sequences: 5004
Number of extensions: 54917
Number of successful extensions: 148
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 355273338
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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