Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30107
(778 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase, p... 119 2e-27
At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, p... 117 8e-27
At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase, p... 116 2e-26
At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase, p... 75 4e-14
At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase (L... 69 5e-12
At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein ... 48 9e-06
At3g06850.2 68416.m00813 branched chain alpha-keto acid dehydrog... 38 0.007
At3g06850.1 68416.m00812 branched chain alpha-keto acid dehydrog... 38 0.007
At4g19515.1 68417.m02871 disease resistance family protein simil... 32 0.49
At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP... 30 2.0
At3g15690.1 68416.m01988 biotin carboxyl carrier protein of acet... 29 3.4
At5g57550.1 68418.m07190 xyloglucan:xyloglucosyl transferase / x... 29 4.5
At5g23110.1 68418.m02703 zinc finger (C3HC4-type RING finger) fa... 29 4.5
At3g15690.2 68416.m01989 biotin carboxyl carrier protein of acet... 29 4.5
At1g66270.2 68414.m07524 beta-glucosidase (PSR3.2) nearly identi... 28 6.0
At1g66270.1 68414.m07523 beta-glucosidase (PSR3.2) nearly identi... 28 6.0
At4g33200.1 68417.m04727 myosin, putative similar to myosin (GI:... 28 7.9
At1g68270.1 68414.m07798 AMP-dependent synthetase and ligase fam... 28 7.9
>At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
[Zea mays] GI:5669871; contains Pfam profiles PF00198:
2-oxo acid dehydrogenases acyltransferase (catalytic
domain), PF00364: Biotin-requiring enzyme, PF02817: e3
binding domain
Length = 539
Score = 119 bits (286), Expect = 2e-27
Identities = 55/110 (50%), Positives = 78/110 (70%), Gaps = 3/110 (2%)
Frame = +3
Query: 261 SSLPSHIKVNLPALSPTMESGSIVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEEGYL 440
S LP H ++ +P+LSPTM G+I W KKEGDK++ G++LCE+ETDKAT+ E EEG+L
Sbjct: 106 SDLPPHQEIGMPSLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGFL 165
Query: 441 AKILIPAGTKGVPVGKLLCIIVGDQNDVAAFKDF--KDDSSPATPQ-KPA 581
AKI+ G K + VG+++ I V D++D+ FKD+ D+ PA P+ KPA
Sbjct: 166 AKIVKEEGAKEIQVGEVIAITVEDEDDIQKFKDYTPSSDTGPAAPEAKPA 215
>At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide acetyltransferase
(E2) subunit of PDC [Arabidopsis thaliana] GI:559395;
contains Pfam profiles PF00198: 2-oxo acid
dehydrogenases acyltransferase (catalytic domain),
PF00364: Biotin-requiring enzyme, PF02817: e3 binding
domain; supporting cDNA
gi|5881964|gb|AF066080.1|AF066080
Length = 637
Score = 117 bits (282), Expect = 8e-27
Identities = 56/99 (56%), Positives = 69/99 (69%)
Frame = +3
Query: 261 SSLPSHIKVNLPALSPTMESGSIVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEEGYL 440
S LP H+ + +PALSPTM G+I W KKEGDK+ GD++ EIETDKAT+ FE+ EEGYL
Sbjct: 207 SDLPPHVVLEMPALSPTMNQGNIAKWWKKEGDKIEVGDVIGEIETDKATLEFESLEEGYL 266
Query: 441 AKILIPAGTKGVPVGKLLCIIVGDQNDVAAFKDFKDDSS 557
AKILIP G+K V VGK + +IV D + A K SS
Sbjct: 267 AKILIPEGSKDVAVGKPIALIVEDAESIEAIKSSSAGSS 305
Score = 105 bits (253), Expect = 2e-23
Identities = 45/78 (57%), Positives = 63/78 (80%)
Frame = +3
Query: 291 LPALSPTMESGSIVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPAGTK 470
+PALSPTM G++V W KKEGDK+ GD+LCEIETDKAT+ FE+ EEG+LAKIL+ G+K
Sbjct: 90 MPALSPTMSHGNVVKWMKKEGDKVEVGDVLCEIETDKATVEFESQEEGFLAKILVTEGSK 149
Query: 471 GVPVGKLLCIIVGDQNDV 524
+PV + + I+V +++D+
Sbjct: 150 DIPVNEPIAIMVEEEDDI 167
>At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
GI:5669871 [Zea mays]; contains Pfam profiles PF00198:
2-oxo acid dehydrogenases acyltransferase (catalytic
domain), PF00364: Biotin-requiring enzyme, PF02817: e3
binding domain
Length = 539
Score = 116 bits (278), Expect = 2e-26
Identities = 53/106 (50%), Positives = 74/106 (69%), Gaps = 1/106 (0%)
Frame = +3
Query: 261 SSLPSHIKVNLPALSPTMESGSIVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEEGYL 440
S LP H ++ +P+LSPTM G+I W KKEGDK++ G++LCE+ETDKAT+ E EEGYL
Sbjct: 106 SDLPPHQEIGMPSLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGYL 165
Query: 441 AKILIPAGTKGVPVGKLLCIIVGDQNDVAAFKDFKDDS-SPATPQK 575
AKI+ G+K + VG+++ I V D+ D+ FKD+ S + A P K
Sbjct: 166 AKIVKAEGSKEIQVGEVIAITVEDEEDIGKFKDYTPSSTADAAPTK 211
>At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963; contains Pfam
profiles PF00198: 2-oxo acid dehydrogenases
acyltransferase (catalytic domain), PF00364:
Biotin-requiring enzyme, PF02817: e3 binding domain
Length = 465
Score = 75.4 bits (177), Expect = 4e-14
Identities = 38/86 (44%), Positives = 57/86 (66%), Gaps = 1/86 (1%)
Frame = +3
Query: 282 KVNLPALSPTMESGSIVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPA 461
++ +PALS TM G IVSW K EGDKL++G+ + +E+DKA M ET +GYLA I++
Sbjct: 41 EIFMPALSSTMTEGKIVSWVKSEGDKLNKGESVVVVESDKADMDVETFYDGYLAAIMVEE 100
Query: 462 GTKGV-PVGKLLCIIVGDQNDVAAFK 536
G GV PVG + ++ ++++A K
Sbjct: 101 G--GVAPVGSAIALLAETEDEIADAK 124
>At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase
(LTA2) identical to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963
Length = 480
Score = 68.5 bits (160), Expect = 5e-12
Identities = 34/85 (40%), Positives = 54/85 (63%)
Frame = +3
Query: 282 KVNLPALSPTMESGSIVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPA 461
++ +PALS TM G IVSW K EG+KL++G+ + +E+DKA M ET +GYLA I++
Sbjct: 57 EIFMPALSSTMTEGKIVSWIKTEGEKLAKGESVVVVESDKADMDVETFYDGYLAAIVVGE 116
Query: 462 GTKGVPVGKLLCIIVGDQNDVAAFK 536
G + PVG + ++ + ++ K
Sbjct: 117 G-ETAPVGAAIGLLAETEAEIEEAK 140
>At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein
similar to SP|Q01205 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Rattus norvegicus}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 464
Score = 47.6 bits (108), Expect = 9e-06
Identities = 31/133 (23%), Positives = 59/133 (44%), Gaps = 5/133 (3%)
Frame = +3
Query: 210 NQTVLSTPQWTVQMRYYSSLPSH----IKVNLPALSPTMESGSIVSWEKKEGDKLSEGDL 377
++T+++ Q T R+ S ++ +P + ++ G++ ++ KK GD++ +
Sbjct: 67 SRTIINGYQGTALQRWVRPFSSDSGDVVEAVVPHMGESITDGTLAAFLKKPGDRVEADEA 126
Query: 378 LCEIETDKATMGFETPEEGYLAKILIPAGTKGVPVGKLLCIIVGDQNDVAAFKDFKDDSS 557
+ +IETDK T+ +P G + + L+ G P K+ I K
Sbjct: 127 IAQIETDKVTIDIASPASGVIQEFLVKEGDTVEPGNKVARISTSADAVSHVAPSEKAPEK 186
Query: 558 PA-TPQKPASQDK 593
PA P PA + K
Sbjct: 187 PAPKPSPPAEKPK 199
>At3g06850.2 68416.m00813 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 37.9 bits (84), Expect = 0.007
Identities = 23/66 (34%), Positives = 37/66 (56%), Gaps = 1/66 (1%)
Frame = +3
Query: 327 IVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPAGTKGVPVGK-LLCII 503
++ W KEGD + E LCE+++DKAT+ + +G +A I G + VG+ L+ +
Sbjct: 92 LLKWFVKEGDSVEEFQPLCEVQSDKATIEITSRFKGKVALISHSPGDI-IKVGETLVRLA 150
Query: 504 VGDQND 521
V D D
Sbjct: 151 VEDSQD 156
>At3g06850.1 68416.m00812 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 37.9 bits (84), Expect = 0.007
Identities = 23/66 (34%), Positives = 37/66 (56%), Gaps = 1/66 (1%)
Frame = +3
Query: 327 IVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPAGTKGVPVGK-LLCII 503
++ W KEGD + E LCE+++DKAT+ + +G +A I G + VG+ L+ +
Sbjct: 92 LLKWFVKEGDSVEEFQPLCEVQSDKATIEITSRFKGKVALISHSPGDI-IKVGETLVRLA 150
Query: 504 VGDQND 521
V D D
Sbjct: 151 VEDSQD 156
>At4g19515.1 68417.m02871 disease resistance family protein similar
to downy mildew resistance protein RPP5 [Arabidopsis
thaliana] GI:6449046; contains Pfam profile PF01582: TIR
domain
Length = 524
Score = 31.9 bits (69), Expect = 0.49
Identities = 14/44 (31%), Positives = 24/44 (54%)
Frame = +3
Query: 93 RNQILSDGLKKAIRSNITRCISTELAKRKVTNKLLEHAQNQTVL 224
R Q +SD LKK + I CI ++ R + NK+++ + V+
Sbjct: 270 RKQFISDFLKKLVYKGIRICIGDKILSRSLINKVIKESSIAVVV 313
>At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP2)
identical to biotin carboxyl carrier protein isoform 2
[Arabidopsis thaliana] gi|8886869|gb|AAF80592
Length = 255
Score = 29.9 bits (64), Expect = 2.0
Identities = 18/53 (33%), Positives = 27/53 (50%)
Frame = +3
Query: 345 KEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPAGTKGVPVGKLLCII 503
K GDK+ +G ++C IE K E + G + ++L G K V V L +I
Sbjct: 202 KVGDKVQKGQIVCIIEAMKLMNEIEAEKSGTIMELLAEDG-KPVSVDTPLFVI 253
>At3g15690.1 68416.m01988 biotin carboxyl carrier protein of
acetyl-CoA carboxylase-related contains weak similarity
to Biotin carboxyl carrier protein of acetyl-CoA
carboxylase, chloroplast precursor (BCCP)
(Swiss-Prot:Q42533) [Arabidopsis thaliana]
Length = 247
Score = 29.1 bits (62), Expect = 3.4
Identities = 16/42 (38%), Positives = 21/42 (50%)
Frame = +3
Query: 345 KEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPAGTK 470
KE D++ EG +LC IE E+ G + KIL G K
Sbjct: 201 KEKDQVKEGQILCYIEQLGGQFPIESDVTGEVVKILREDGGK 242
>At5g57550.1 68418.m07190 xyloglucan:xyloglucosyl transferase /
xyloglucan endotransglycosylase / endo-xyloglucan
transferase (XTR3) identical to endoxyloglucan
transferase GI:5533317 from [Arabidopsis thaliana]
Length = 284
Score = 28.7 bits (61), Expect = 4.5
Identities = 19/66 (28%), Positives = 33/66 (50%), Gaps = 6/66 (9%)
Frame = +3
Query: 294 PALSPTMESGSIVSWEKKEGDKLSEGDLLCEIETDKAT-MGFETPEEGYLAKI-----LI 455
P + T ++ ++W G L+ G+LL + D+A+ GF+T +E KI L+
Sbjct: 24 PVFAGTFDTEFDITWGDGRGKVLNNGELL-TLSLDRASGSGFQTKKEYLFGKIDMQLKLV 82
Query: 456 PAGTKG 473
P + G
Sbjct: 83 PGNSAG 88
>At5g23110.1 68418.m02703 zinc finger (C3HC4-type RING finger) family
protein contains Pfam profile: PF00097 zinc finger, C3HC4
type (RING finger)
Length = 4706
Score = 28.7 bits (61), Expect = 4.5
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = +3
Query: 279 IKVNLPALSPTMESGSIVSWEKKEGDKLSEG 371
++V L L P + G IV+W+ K+GDKL G
Sbjct: 4475 VQVQLHPLRPFYK-GEIVAWKIKQGDKLRYG 4504
>At3g15690.2 68416.m01989 biotin carboxyl carrier protein of
acetyl-CoA carboxylase-related contains weak similarity
to Biotin carboxyl carrier protein of acetyl-CoA
carboxylase, chloroplast precursor (BCCP)
(Swiss-Prot:Q42533) [Arabidopsis thaliana]
Length = 263
Score = 28.7 bits (61), Expect = 4.5
Identities = 18/47 (38%), Positives = 23/47 (48%)
Frame = +3
Query: 345 KEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPAGTKGVPVG 485
KE D++ EG +LC IE E+ G + KIL G PVG
Sbjct: 201 KEKDQVKEGQILCYIEQLGGQFPIESDVTGEVVKIL---REDGEPVG 244
>At1g66270.2 68414.m07524 beta-glucosidase (PSR3.2) nearly identical
to GI:2286069 from (Arabidopsis thaliana) (Plant Mol.
Biol. 34 (1), 57-68 (1997)); similar to thioglucoside
glucohydrolase (GI:984052) [Arabidopsis thaliana]
Length = 522
Score = 28.3 bits (60), Expect = 6.0
Identities = 14/45 (31%), Positives = 22/45 (48%)
Frame = +3
Query: 243 VQMRYYSSLPSHIKVNLPALSPTMESGSIVSWEKKEGDKLSEGDL 377
V + YY+S S+ P+ + S+VSWE K D + G +
Sbjct: 339 VGLNYYTSTFSNYNEKPDPSKPSWKQDSLVSWEPKNVDHSAIGSM 383
>At1g66270.1 68414.m07523 beta-glucosidase (PSR3.2) nearly identical
to GI:2286069 from (Arabidopsis thaliana) (Plant Mol.
Biol. 34 (1), 57-68 (1997)); similar to thioglucoside
glucohydrolase (GI:984052) [Arabidopsis thaliana]
Length = 524
Score = 28.3 bits (60), Expect = 6.0
Identities = 14/45 (31%), Positives = 22/45 (48%)
Frame = +3
Query: 243 VQMRYYSSLPSHIKVNLPALSPTMESGSIVSWEKKEGDKLSEGDL 377
V + YY+S S+ P+ + S+VSWE K D + G +
Sbjct: 341 VGLNYYTSTFSNYNEKPDPSKPSWKQDSLVSWEPKNVDHSAIGSM 385
>At4g33200.1 68417.m04727 myosin, putative similar to myosin
(GI:433663) [Arabidopsis thaliana]
Length = 1522
Score = 27.9 bits (59), Expect = 7.9
Identities = 15/63 (23%), Positives = 26/63 (41%)
Frame = +3
Query: 102 ILSDGLKKAIRSNITRCISTELAKRKVTNKLLEHAQNQTVLSTPQWTVQMRYYSSLPSHI 281
++ D LKK + + CI A R + K + QW +++ SL S +
Sbjct: 1260 LIRDNLKKELSPLLGSCIQAPKASRGIAGKSRSPGGVPQQSPSSQWESILKFLDSLMSRL 1319
Query: 282 KVN 290
+ N
Sbjct: 1320 REN 1322
>At1g68270.1 68414.m07798 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 535
Score = 27.9 bits (59), Expect = 7.9
Identities = 11/46 (23%), Positives = 24/46 (52%)
Frame = -3
Query: 488 FPDRHSLCTGRNQYFGQVTFFRCLKAHSCFVSFNLAQKIAFTKFVP 351
+P+R S+ G+ ++ T+ RC + + +S N+A+ + P
Sbjct: 27 YPNRTSIIYGQTRFTWPQTYDRCCRLAASLISLNIAKNDVVSVVAP 72
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,529,083
Number of Sequences: 28952
Number of extensions: 306988
Number of successful extensions: 909
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 884
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 908
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1736283200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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