Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30072
(564 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces po... 219 3e-58
SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosacch... 190 9e-50
SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces... 93 3e-20
SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizos... 34 0.017
SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr ... 27 1.4
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 1.9
SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic... 27 1.9
SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase Byr2... 27 2.5
SPAC27F1.08 |pdt1||Nramp family manganese ion transporter|Schizo... 27 2.5
SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyce... 26 3.3
SPBP23A10.14c |ell1||RNA polymerase II transcription elongation ... 26 3.3
SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large... 25 7.7
SPAP8A3.03 |||ZIP zinc transporter 1|Schizosaccharomyces pombe|c... 25 7.7
>SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 161
Score = 219 bits (534), Expect = 3e-58
Identities = 109/152 (71%), Positives = 130/152 (85%)
Frame = +1
Query: 73 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAI 252
P+Y PFFGVMG +AI+F++ GAAYGTAK+G GI+AM V+RP+LI+K+ IPVVMAGIIAI
Sbjct: 7 PVYAPFFGVMGCTAAIVFASFGAAYGTAKAGVGISAMGVLRPDLIVKNTIPVVMAGIIAI 66
Query: 253 YGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVRFSGLAAGFAISIVGDAXVRGTAQQP 432
YGLVV+VLI+G L++ + LY GFI LGAGL+V +GLAAGFAI IVGDA VRGTAQQP
Sbjct: 67 YGLVVSVLISGNLKQILS--LYSGFIQLGAGLSVGLAGLAAGFAIGIVGDAGVRGTAQQP 124
Query: 433 KLFVGMILILIFAEVLGLYGLIVAIYLYTK*T 528
+LFV MILILIFAEVLGLYGLIVA+ L T+ T
Sbjct: 125 RLFVAMILILIFAEVLGLYGLIVALLLNTRAT 156
>SPAC732.01 |vma11||V-type ATPase proteolipid
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 162
Score = 190 bits (464), Expect = 9e-50
Identities = 89/150 (59%), Positives = 115/150 (76%)
Frame = +1
Query: 73 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAI 252
PIY FFG G ++++FS LGA YGTA +G GIAA+ RPE++MKS+IPVVM+GII +
Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAVGAFRPEIVMKSLIPVVMSGIIGV 66
Query: 253 YGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVRFSGLAAGFAISIVGDAXVRGTAQQP 432
YGLV++VLIAG + +Y L+ GFIHL AGLAV +G+AAG+AI +VGD V+ +Q
Sbjct: 67 YGLVMSVLIAGDMSPDNDYSLFSGFIHLSAGLAVGLTGVAAGYAIGVVGDRGVQSFMRQD 126
Query: 433 KLFVGMILILIFAEVLGLYGLIVAIYLYTK 522
++FV M+LILIFAEVLGLYGLIV + L TK
Sbjct: 127 RIFVSMVLILIFAEVLGLYGLIVGLILQTK 156
Score = 38.7 bits (86), Expect = 6e-04
Identities = 18/79 (22%), Positives = 43/79 (54%), Gaps = 1/79 (1%)
Frame = +1
Query: 310 PLYKGFIHL-GAGLAVRFSGLAAGFAISIVGDAXVRGTAQQPKLFVGMILILIFAEVLGL 486
P+Y F G ++ FS L AG+ ++ G A +P++ + ++ ++ + ++G+
Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAVGAFRPEIVMKSLIPVVMSGIIGV 66
Query: 487 YGLIVAIYLYTK*TPWNTH 543
YGL++++ + +P N +
Sbjct: 67 YGLVMSVLIAGDMSPDNDY 85
>SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 199
Score = 92.7 bits (220), Expect = 3e-20
Identities = 47/147 (31%), Positives = 79/147 (53%), Gaps = 6/147 (4%)
Frame = +1
Query: 91 FGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIYGLVVA 270
+G++G AS + F +GAA+G GT I +V P + K++I ++ ++AIY L++A
Sbjct: 45 WGLLGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIFCEVVAIYSLIIA 104
Query: 271 VLIAGALQE--PANY----PLYKGFIHLGAGLAVRFSGLAAGFAISIVGDAXVRGTAQQP 432
++ + + + PA + Y GF G+ V L G + I G + AQ
Sbjct: 105 IVFSAKINDINPAGFYTKSHYYTGFALFWGGITVGLCNLICGVCVGITGSSAALADAQDA 164
Query: 433 KLFVGMILILIFAEVLGLYGLIVAIYL 513
LFV ++++ IF VLGL+GLIV + +
Sbjct: 165 SLFVKVLVVEIFGSVLGLFGLIVGLLI 191
>SPAC212.04c |||S. pombe specific DUF999 family protein
1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 288
Score = 33.9 bits (74), Expect = 0.017
Identities = 21/68 (30%), Positives = 31/68 (45%)
Frame = +1
Query: 217 IIPVVMAGIIAIYGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVRFSGLAAGFAISIV 396
II +AG+IA + +++ IAG + G ++ G L + LA GF I
Sbjct: 193 IITATIAGVIAAFSVIITATIAGVIAAMV------GILYFGHWLVYKILILAFGFKIVTS 246
Query: 397 GDAXVRGT 420
GD V T
Sbjct: 247 GDVCVSNT 254
>SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr
1|||Manual
Length = 249
Score = 27.5 bits (58), Expect = 1.4
Identities = 22/72 (30%), Positives = 32/72 (44%)
Frame = +1
Query: 76 IYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIY 255
+YGPF+ A+ FS Y +G G + S+ K +I A II Y
Sbjct: 87 LYGPFWITTTVIQALFFSNSITEYARYATGHGTSGYSI-------KKLISA--ASIIYGY 137
Query: 256 GLVVAVLIAGAL 291
++AVL+ G L
Sbjct: 138 TTIIAVLLWGIL 149
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.1 bits (57), Expect = 1.9
Identities = 19/57 (33%), Positives = 27/57 (47%)
Frame = -2
Query: 491 P*RPNTSAKIRIRIIPTNNLGC*AVPRTXASPTMLMAKPAARPENLTAKPAPKWMNP 321
P RP ++A ++ PT +VP ++P M P+A P L A AP NP
Sbjct: 1691 PVRPQSAAPPQMSA-PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic
subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1729
Score = 27.1 bits (57), Expect = 1.9
Identities = 21/95 (22%), Positives = 47/95 (49%), Gaps = 6/95 (6%)
Frame = +1
Query: 52 NKMAENNP---IYGPFFG-VMGAASAIIFSALGAAYGTAKSGTGIAAMSVMR-PELIMKS 216
+K+A ++P I F+G ++G + F+ + + ++ G S LI+ S
Sbjct: 1424 DKIAMDSPRARITTMFYGEILGPLGTLFFTCIPFLFINSQPGNDDETQSTNAFIRLIIMS 1483
Query: 217 IIPVVMAGIIAIYGLVVAVLIAGALQEPA-NYPLY 318
+ P+V++ IIA + + +++ L + + Y +Y
Sbjct: 1484 VAPLVLSAIIAFFFFCLGIMLRPILGDRSKTYGVY 1518
>SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase
Byr2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 659
Score = 26.6 bits (56), Expect = 2.5
Identities = 19/74 (25%), Positives = 34/74 (45%)
Frame = -1
Query: 513 QVDGDDKSVKTQYFSENKNKNHSDE*LRLLSSTTHACISHDAYGETGSQTRESYSQTSTQ 334
Q +G++KS K FSE+ + NH + L ++ + I + S T E +
Sbjct: 329 QSNGNEKSSKYNVFSESAHGNH--QVLSFSPGSSPSFIEQPSPISPTSTTSEDTNTLEED 386
Query: 333 VDEPFVKGVVGWLL 292
D+ +K + G L+
Sbjct: 387 TDDQSIKWIRGALI 400
>SPAC27F1.08 |pdt1||Nramp family manganese ion
transporter|Schizosaccharomyces pombe|chr 1|||Manual
Length = 521
Score = 26.6 bits (56), Expect = 2.5
Identities = 13/41 (31%), Positives = 22/41 (53%), Gaps = 4/41 (9%)
Frame = +1
Query: 307 YPLYKGFIHLGAG----LAVRFSGLAAGFAISIVGDAXVRG 417
Y L K ++ + G +A+ FSG++AG+ +I G G
Sbjct: 359 YDLLKEYVSISCGRLFAVALLFSGMSAGYVCTIAGQIVSEG 399
>SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 760
Score = 26.2 bits (55), Expect = 3.3
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = -1
Query: 330 DEPFVKGVVGWLLEGTSNQDSHDQTVDGNNTRHDDRND 217
DEPF+K +L+ SN++S+ VD N+ D +D
Sbjct: 48 DEPFLKSKYMDILQKISNRESNVINVDLNDLYEFDPSD 85
>SPBP23A10.14c |ell1||RNA polymerase II transcription elongation
factor SpELL|Schizosaccharomyces pombe|chr 2|||Manual
Length = 533
Score = 26.2 bits (55), Expect = 3.3
Identities = 11/28 (39%), Positives = 19/28 (67%)
Frame = +3
Query: 474 SIGSLRTYRRHLPVHKINALEHTPLPSP 557
SI S+ +Y++ + K+NA ++PL SP
Sbjct: 332 SISSVSSYQQQHRIPKLNASNYSPLLSP 359
>SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large
subunit Rpc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1405
Score = 25.0 bits (52), Expect = 7.7
Identities = 8/19 (42%), Positives = 13/19 (68%)
Frame = -3
Query: 397 PRCLWRNRQPDQRILQPNQ 341
PRCLW +Q +++PN+
Sbjct: 582 PRCLWTGKQVFTVLMKPNR 600
>SPAP8A3.03 |||ZIP zinc transporter 1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 453
Score = 25.0 bits (52), Expect = 7.7
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +1
Query: 448 MILILIFAEVLGLYGLIVAIYLYT 519
++++ + V GL G IVA Y+YT
Sbjct: 358 VLVLQMITMVTGLLGAIVATYIYT 381
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,556,128
Number of Sequences: 5004
Number of extensions: 52976
Number of successful extensions: 165
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 163
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 238029836
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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