BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= maV30071 (669 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 212 2e-55 At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 151 5e-37 At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 151 5e-37 At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 148 3e-36 At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 93 2e-19 At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 52 5e-07 At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 52 5e-07 At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 48 4e-06 At5g13650.2 68418.m01585 elongation factor family protein contai... 42 5e-04 At5g13650.1 68418.m01584 elongation factor family protein contai... 42 5e-04 At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 31 0.92 At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634... 29 2.8 At2g35490.1 68415.m04347 plastid-lipid associated protein PAP, p... 28 6.5 At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /... 27 8.5 At3g63180.1 68416.m07097 expressed protein 27 8.5 >At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, putative similar to ELONGATION FACTOR 2 GB:O14460 from [Schizosaccharomyces pombe] Length = 843 Score = 212 bits (518), Expect = 2e-55 Identities = 93/147 (63%), Positives = 118/147 (80%) Frame = +3 Query: 3 FNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYG 182 F + DV LH+DAIHRGGGQ+IPT RR +YA +TA+PRL+EPVY+ EIQ PE A+GGIY Sbjct: 687 FEVCDVVLHSDAIHRGGGQVIPTARRVIYASQITAKPRLLEPVYMVEIQAPEGALGGIYS 746 Query: 183 VLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLP 362 VLN++RGHVFEE Q GTP++ +KAYLPV ESFGF++ LR+ T GQAFPQCVFDHW+++ Sbjct: 747 VLNQKRGHVFEEMQRPGTPLYNIKAYLPVVESFGFSSQLRAATSGQAFPQCVFDHWEMMS 806 Query: 363 GDPCEPQSKPYNVVQETRKRKGLKEGL 443 DP EP ++ +V + RKRKGLKE + Sbjct: 807 SDPLEPGTQASVLVADIRKRKGLKEAM 833 >At1g06220.2 68414.m00656 elongation factor Tu family protein similar to Cryptosporidium parvum elongation factor-2 GB:U21667 GI:706974 from [Cryptosporidium parvum] Length = 987 Score = 151 bits (365), Expect = 5e-37 Identities = 79/157 (50%), Positives = 97/157 (61%), Gaps = 12/157 (7%) Frame = +3 Query: 3 FNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYG 182 F I D + + +HRG GQ+IPT RR Y+ L A PRLMEPVY EIQ P V IY Sbjct: 803 FKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYT 862 Query: 183 VLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLP 362 VL+RRRGHV + GTP +IVKA+LPV ESFGF DLR +T GQAF VFDHW ++P Sbjct: 863 VLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDHWAIVP 922 Query: 363 GDPCEP--QSKPY----------NVVQETRKRKGLKE 437 GDP + Q +P + +TR+RKG+ E Sbjct: 923 GDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRKGMSE 959 >At1g06220.1 68414.m00655 elongation factor Tu family protein similar to Cryptosporidium parvum elongation factor-2 GB:U21667 GI:706974 from [Cryptosporidium parvum] Length = 987 Score = 151 bits (365), Expect = 5e-37 Identities = 79/157 (50%), Positives = 97/157 (61%), Gaps = 12/157 (7%) Frame = +3 Query: 3 FNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYG 182 F I D + + +HRG GQ+IPT RR Y+ L A PRLMEPVY EIQ P V IY Sbjct: 803 FKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYT 862 Query: 183 VLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLP 362 VL+RRRGHV + GTP +IVKA+LPV ESFGF DLR +T GQAF VFDHW ++P Sbjct: 863 VLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDHWAIVP 922 Query: 363 GDPCEP--QSKPY----------NVVQETRKRKGLKE 437 GDP + Q +P + +TR+RKG+ E Sbjct: 923 GDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRKGMSE 959 >At5g25230.1 68418.m02991 elongation factor Tu family protein translation Elongation Factor 2, Schizosaccharomyces pombe, PIR:T39902 Length = 973 Score = 148 bits (359), Expect = 3e-36 Identities = 78/157 (49%), Positives = 97/157 (61%), Gaps = 12/157 (7%) Frame = +3 Query: 3 FNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYG 182 F I D + + +HRG GQ+IPT RR Y+ L A PRLMEPVY EIQ P V IY Sbjct: 789 FKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYT 848 Query: 183 VLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLP 362 VL+RRRG+V + GTP +IVKA+LPV ESFGF DLR +T GQAF VFDHW ++P Sbjct: 849 VLSRRRGYVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDHWAIVP 908 Query: 363 GDPCEP--QSKPY----------NVVQETRKRKGLKE 437 GDP + Q +P + +TR+RKG+ E Sbjct: 909 GDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRKGMSE 945 >At3g22980.1 68416.m02898 elongation factor Tu family protein similar to eukaryotic translation elongation factor 2 GB:NP_001952 [Homo sapiens] Length = 1015 Score = 93.1 bits (221), Expect = 2e-19 Identities = 41/106 (38%), Positives = 61/106 (57%) Frame = +3 Query: 54 GQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAG 233 GQ++ + A +L PR++E +Y CE+ +G +Y VL+RRR + +E G Sbjct: 853 GQVMTAVKDACRAAVLQTNPRIVEAMYFCELNTAPEYLGPMYAVLSRRRARILKEEMQEG 912 Query: 234 TPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDP 371 + +F V AY+PV+ESFGF +LR T G A V HW++L DP Sbjct: 913 SSLFTVHAYVPVSESFGFADELRKGTSGGASALMVLSHWEMLEEDP 958 >At2g45030.1 68415.m05606 mitochondrial elongation factor, putative similar to SP|P25039 Elongation factor G 1, mitochondrial precursor (mEF-G-1) {Saccharomyces cerevisiae}; contains Pfam profiles PF00009: Elongation factor Tu GTP binding domain, PF03764: Elongation factor G domain IV, PF00679: Elongation factor G C-terminus Length = 754 Score = 51.6 bits (118), Expect = 5e-07 Identities = 25/76 (32%), Positives = 42/76 (55%) Frame = +3 Query: 93 CLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVN 272 C A+P ++EPV L E++ P G + G +N+R+G + Q ++ A +P+N Sbjct: 649 CYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLN 706 Query: 273 ESFGFTADLRSNTGGQ 320 FG++ LRS T G+ Sbjct: 707 NMFGYSTSLRSMTQGK 722 >At1g45332.1 68414.m05195 mitochondrial elongation factor, putative similar to mitochondrial elongation factor GI:3917 from [Saccharomyces cerevisiae] Length = 754 Score = 51.6 bits (118), Expect = 5e-07 Identities = 25/76 (32%), Positives = 42/76 (55%) Frame = +3 Query: 93 CLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVN 272 C A+P ++EPV L E++ P G + G +N+R+G + Q ++ A +P+N Sbjct: 649 CYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLN 706 Query: 273 ESFGFTADLRSNTGGQ 320 FG++ LRS T G+ Sbjct: 707 NMFGYSTSLRSMTQGK 722 >At1g62750.1 68414.m07082 elongation factor Tu family protein similar to elongation factor G SP:P34811 [Glycine max (Soybean)] Length = 783 Score = 48.4 bits (110), Expect = 4e-06 Identities = 27/86 (31%), Positives = 44/86 (51%) Frame = +3 Query: 105 AQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFG 284 A PR++EP+ E+ PE +G + G LN RRG + G + +V + +P+ E F Sbjct: 683 AGPRMLEPIMRVEVVTPEEHLGDVIGDLNSRRGQINSFGDKPG-GLKVVDSLVPLAEMFQ 741 Query: 285 FTADLRSNTGGQAFPQCVFDHWQVLP 362 + + LR T G+A + V+P Sbjct: 742 YVSTLRGMTKGRASYTMQLAKFDVVP 767 >At5g13650.2 68418.m01585 elongation factor family protein contains Pfam profiles: PF00009 elongation factor Tu GTP binding domain,PF00679 elongation factor G C-terminus, PF03144 elongation factor Tu domain 2 Length = 676 Score = 41.5 bits (93), Expect = 5e-04 Identities = 21/85 (24%), Positives = 39/85 (45%) Frame = +3 Query: 114 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTA 293 +L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G Sbjct: 473 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRN 532 Query: 294 DLRSNTGGQAFPQCVFDHWQVLPGD 368 + + + G A VFD + GD Sbjct: 533 AILTASRGTAILNTVFDSYGPWAGD 557 >At5g13650.1 68418.m01584 elongation factor family protein contains Pfam profiles: PF00009 elongation factor Tu GTP binding domain,PF00679 elongation factor G C-terminus, PF03144 elongation factor Tu domain 2 Length = 675 Score = 41.5 bits (93), Expect = 5e-04 Identities = 21/85 (24%), Positives = 39/85 (45%) Frame = +3 Query: 114 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTA 293 +L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G Sbjct: 472 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRN 531 Query: 294 DLRSNTGGQAFPQCVFDHWQVLPGD 368 + + + G A VFD + GD Sbjct: 532 AILTASRGTAILNTVFDSYGPWAGD 556 >At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-binding protein GUF1 - Saccharomyces cerevisiae, PIR:S50374 Length = 661 Score = 30.7 bits (66), Expect = 0.92 Identities = 21/68 (30%), Positives = 32/68 (47%), Gaps = 1/68 (1%) Frame = +3 Query: 123 EPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNE-SFGFTADL 299 EP + I P VG + + + RRG E + + +F+ K LP+ E F +L Sbjct: 466 EPTVIATIILPSEYVGAVINLCSDRRGQQLEYTFIDAQRVFL-KYQLPLREIVVDFYDEL 524 Query: 300 RSNTGGQA 323 +S T G A Sbjct: 525 KSITSGYA 532 >At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634: Arabidopsis thaliana protein of unknown function (DUF794) Length = 340 Score = 29.1 bits (62), Expect = 2.8 Identities = 11/17 (64%), Positives = 12/17 (70%) Frame = -1 Query: 408 PVQRCRACSEVRTGLQG 358 PV RCR C+EV G QG Sbjct: 62 PVHRCRLCAEVHIGKQG 78 >At2g35490.1 68415.m04347 plastid-lipid associated protein PAP, putative similar to plastid-lipid associated protein PAP3 [Brassica rapa] GI:14248552; contains Pfam profile PF04755: PAP_fibrillin Length = 376 Score = 27.9 bits (59), Expect = 6.5 Identities = 12/22 (54%), Positives = 13/22 (59%) Frame = +3 Query: 336 VFDHWQVLPGDPCEPQSKPYNV 401 V D W PGD EP S+P NV Sbjct: 71 VTDEWGEKPGDANEPDSQPDNV 92 >At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein weak similarity to polygalacturonase PG1 [Glycine max] GI:5669846; contains PF00295: Glycosyl hydrolases family 28 Length = 444 Score = 27.5 bits (58), Expect = 8.5 Identities = 11/22 (50%), Positives = 17/22 (77%), Gaps = 1/22 (4%) Frame = +2 Query: 197 SWSRFRRVPGGRY-TYVHCEGL 259 S+ R R +PGGRY +++H +GL Sbjct: 123 SYGRGRELPGGRYMSFIHGDGL 144 >At3g63180.1 68416.m07097 expressed protein Length = 978 Score = 27.5 bits (58), Expect = 8.5 Identities = 10/35 (28%), Positives = 21/35 (60%) Frame = +1 Query: 256 PTYLSMSRSVLLPICVPTPADRPSRSAYSTIGRSS 360 P ++ + L P+ VP+ + RP++S + +G +S Sbjct: 539 PAFIYPANHHLQPVMVPSKSSRPTKSPHLAVGLAS 573 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,900,367 Number of Sequences: 28952 Number of extensions: 316431 Number of successful extensions: 755 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 731 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 750 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1412971776 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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