BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= maV30069 (658 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g17830.1 68417.m02659 peptidase M20/M25/M40 family protein si... 37 0.014 At1g44820.1 68414.m05134 aminoacylase, putative / N-acyl-L-amino... 35 0.055 At1g44180.1 68414.m05103 aminoacylase, putative / N-acyl-L-amino... 33 0.17 At1g62570.1 68414.m07059 flavin-containing monooxygenase family ... 30 1.6 At4g11030.1 68417.m01794 long-chain-fatty-acid--CoA ligase, puta... 27 8.3 At3g11490.1 68416.m01401 rac GTPase activating protein, putative... 27 8.3 At2g17530.1 68415.m02028 protein kinase family protein identical... 27 8.3 >At4g17830.1 68417.m02659 peptidase M20/M25/M40 family protein similar to acetylornithine deacetylase (Acetylornithinase, AO; N-acetylornithinase, NAO) [Dictyostelium discoideum] SWISS-PROT:P54638 Length = 440 Score = 36.7 bits (81), Expect = 0.014 Identities = 19/39 (48%), Positives = 23/39 (58%) Frame = +3 Query: 360 HLDVQPALKSDGWETEPFELVERNEKLYGRGSTDDKGPV 476 H+DV A D WE +PF L +KL GRG+TD G V Sbjct: 101 HMDVVTA-NPDDWEFDPFSLSIDGDKLRGRGTTDCLGHV 138 >At1g44820.1 68414.m05134 aminoacylase, putative / N-acyl-L-amino-acid amidohydrolase, putative similar to aminoacylase-1 (N-acyl-L-amino-acid amidohydrolase ACY-1)[Homo sapiens] SWISS-PROT:Q03154 Length = 438 Score = 34.7 bits (76), Expect = 0.055 Identities = 24/84 (28%), Positives = 40/84 (47%), Gaps = 1/84 (1%) Frame = +3 Query: 324 NDPKKNTVCIYGHLDVQPALKSDGWETEPFELVERNE-KLYGRGSTDDKGPVLGWLHTIN 500 ++P ++ HLD PA +S+ W PF + + +Y RG+ DDK + +L +I Sbjct: 85 SNPNLPSILFNSHLDSVPA-ESEKWTYPPFSAHKTIDGHIYARGAQDDKCIGVQYLESIR 143 Query: 501 AYKALVLSCLSILKFIFECMEESG 572 K+ S L + + EE G Sbjct: 144 NLKSRGFSPLRTIHISYVPEEEIG 167 >At1g44180.1 68414.m05103 aminoacylase, putative / N-acyl-L-amino-acid amidohydrolase, putative similar to aminoacylase-1 (N-acyl-L-amino-acid amidohydrolase, ACY-1) [Homo sapiens] SWISS-PROT:Q03154 Length = 435 Score = 33.1 bits (72), Expect = 0.17 Identities = 24/82 (29%), Positives = 37/82 (45%), Gaps = 1/82 (1%) Frame = +3 Query: 339 NTVCIYGHLDVQPALKSDGWETEPFELVERNE-KLYGRGSTDDKGPVLGWLHTINAYKAL 515 +++ HLD PA +S+ W PF + +Y RG+ DDK + +L I K+ Sbjct: 92 SSILFNSHLDSVPA-ESEKWIHPPFSAHRTIDGHIYARGAQDDKCIGVQYLEAIRNLKSR 150 Query: 516 VLSCLSILKFIFECMEESGSEG 581 S L + + EE G G Sbjct: 151 SFSPLRTIHISYVPEEEIGGFG 172 >At1g62570.1 68414.m07059 flavin-containing monooxygenase family protein / FMO family protein low similarity to flavin-containing monooxygenase FMO3 [Rattus norvegicus] GI:12006730; contains Pfam profile PF00743: Flavin-binding monooxygenase-like Length = 461 Score = 29.9 bits (64), Expect = 1.6 Identities = 19/64 (29%), Positives = 33/64 (51%) Frame = +3 Query: 27 YSVSSKQVSAKMATEKTLPEIFKYVDQNKDSYKQLLKEAVAIPSVSCDVKYRADCIRMVH 206 + V SK V+A ++ TLP + + +D K SY+ +EA+ IP ++ C + Sbjct: 337 FEVQSKWVAAVLSGRVTLPSVDEMMDDLKLSYE--TQEALGIPKRYTHKLGKSQC-EYLD 393 Query: 207 WMQD 218 W+ D Sbjct: 394 WIAD 397 >At4g11030.1 68417.m01794 long-chain-fatty-acid--CoA ligase, putative / long-chain acyl-CoA synthetase, putative similar to acyl-CoA synthetase (MF7P) gi:1617270 from Brassica napus Length = 666 Score = 27.5 bits (58), Expect = 8.3 Identities = 19/70 (27%), Positives = 33/70 (47%) Frame = +3 Query: 27 YSVSSKQVSAKMATEKTLPEIFKYVDQNKDSYKQLLKEAVAIPSVSCDVKYRADCIRMVH 206 + +S +VS EK +PE+FK N Y +K V+ V + K A+ + +V Sbjct: 145 FIISHAEVSIAFVEEKKIPELFK-TCPNSTKY---MKTVVSFGGVKPEQKEEAEKLGLVI 200 Query: 207 WMQDKLKEVG 236 D+ ++G Sbjct: 201 HSWDEFLKLG 210 >At3g11490.1 68416.m01401 rac GTPase activating protein, putative similar to rac GTPase activating protein 1 GB:AAC62624 GI:3695059 [Lotus japonicus]; contains Pfam profile PF00620: RhoGAP domain Length = 435 Score = 27.5 bits (58), Expect = 8.3 Identities = 14/37 (37%), Positives = 19/37 (51%), Gaps = 1/37 (2%) Frame = +2 Query: 524 LPVNFEVH-FRMYGGIWFRRLDSLLMDKLKPEGFFDS 631 +P N +VH WFR L S ++D L PE +S Sbjct: 198 IPDNIDVHCLASLIKAWFRELPSGVLDSLSPEQVMES 234 >At2g17530.1 68415.m02028 protein kinase family protein identical to SRPK2 [Arabidopsis thaliana] gi|9843645|emb|CAC03676; contains protein kinase domain, Pfam:PF00069 Length = 440 Score = 27.5 bits (58), Expect = 8.3 Identities = 13/40 (32%), Positives = 18/40 (45%) Frame = +2 Query: 254 GCWLSNNRWQRRSTAASFSRRIG**SKKKYSLYLWSFGCT 373 GCW N + T + + S YS+ +WSF CT Sbjct: 263 GCWADNKFAEEIQTRQYRAPEVILQSGYSYSVDMWSFACT 302 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,979,885 Number of Sequences: 28952 Number of extensions: 316599 Number of successful extensions: 770 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 757 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 770 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1373722560 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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