BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= maV30038 (529 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC306.08c |||malate dehydrogenase|Schizosaccharomyces pombe|ch... 140 2e-34 SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 48 7e-07 SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4 desaturase... 27 1.7 SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc... 26 3.0 SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyc... 26 4.0 SPBC36.06c |spo9||farnesyl pyrophosphate synthetase|Schizosaccha... 25 5.3 SPAC11D3.06 |||MatE family transporter|Schizosaccharomyces pombe... 25 7.0 SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 25 9.2 SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces ... 25 9.2 >SPCC306.08c |||malate dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual Length = 341 Score = 140 bits (338), Expect = 2e-34 Identities = 71/133 (53%), Positives = 91/133 (68%), Gaps = 1/133 (0%) Frame = +3 Query: 99 KNFSTTSQRNFKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHVNT 278 ++FSTTS R FK IGQPL++LLK N V+ LAL+DI PGVAAD+ H+NT Sbjct: 19 RSFSTTSSRAFKVAVLGAGGGIGQPLSMLLKLNDKVSELALFDIRGA-PGVAADIGHINT 77 Query: 279 PAKVSGHKGPEE-LSXAIKDADVVVIPXGVPREPGMTRDDLFNTNASIVRDIALSIAXNA 455 + V G+ ++ L A+ ADVV+IP GVPR+PGMTRDDLF TNASIVRD+A + Sbjct: 78 TSNVVGYAPDDKGLEKALNGADVVIIPAGVPRKPGMTRDDLFATNASIVRDLAFAAGETC 137 Query: 456 PKAIVAIITNPVN 494 P+A ++TNPVN Sbjct: 138 PEAKYLVVTNPVN 150 >SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 330 Score = 48.4 bits (110), Expect = 7e-07 Identities = 45/141 (31%), Positives = 63/141 (44%), Gaps = 7/141 (4%) Frame = +3 Query: 93 GAKNFSTTSQRN--FKXXXXXXXXX--IGQPLALLLKQNPLVTRLALYDI-APVTPGVAA 257 G+K FS S R+ FK +G A L + L + + D+ G A Sbjct: 4 GSKVFSNDSVRSSSFKSIKIVIVGAGNVGSTTAFTLLLSGLAAEIVIIDLNKKKAEGEAM 63 Query: 258 DLSHVNTPAKVSGHKGPEELSXA--IKDADVVVIPXGVPREPGMTRDDLFNTNASIVRDI 431 DL+H A +S H+ L KDA VVI G ++PG TR DL N SI ++I Sbjct: 64 DLNHA---APLS-HETRVYLGDYKDCKDATAVVITAGKNQKPGETRMDLLKANISIFKEI 119 Query: 432 ALSIAXNAPKAIVAIITNPVN 494 + AI+ + TNPV+ Sbjct: 120 LREVTKYTKDAILLVATNPVD 140 >SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4 desaturase|Schizosaccharomyces pombe|chr 2|||Manual Length = 362 Score = 27.1 bits (57), Expect = 1.7 Identities = 10/26 (38%), Positives = 14/26 (53%) Frame = +2 Query: 77 PCCTKWCKELFHHITEEL*SGGRWCR 154 P C WC ++ IT+ + G WCR Sbjct: 319 PDCKSWCGIIYQFITDS--NVGMWCR 342 >SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Schizosaccharomyces pombe|chr 2|||Manual Length = 388 Score = 26.2 bits (55), Expect = 3.0 Identities = 19/56 (33%), Positives = 22/56 (39%), Gaps = 1/56 (1%) Frame = +2 Query: 134 SGGRWCRRWYRPAFGPSTEAESSG-DQAGFIRHSACDPRRRSRPFPREHPSQGQRP 298 S G W RR P P+ +G R A DP R R PRE +G P Sbjct: 179 STGDWVRRGPLPPAEPAESPFGKRRTNSGRFRDPARDPSDRVREEPREWVRRGPLP 234 >SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 489 Score = 25.8 bits (54), Expect = 4.0 Identities = 13/33 (39%), Positives = 18/33 (54%) Frame = +2 Query: 65 KTCRPCCTKWCKELFHHITEEL*SGGRWCRRWY 163 K C+ C + C+ +HHI E+L CRR Y Sbjct: 33 KPCQ-CGYRVCRFCWHHIKEDLNGRCPACRRLY 64 >SPBC36.06c |spo9||farnesyl pyrophosphate synthetase|Schizosaccharomyces pombe|chr 2|||Manual Length = 351 Score = 25.4 bits (53), Expect = 5.3 Identities = 10/18 (55%), Positives = 13/18 (72%) Frame = +3 Query: 474 IITNPVNFYSALLPLKCS 527 IIT +FYS LP+KC+ Sbjct: 192 IITYKTSFYSFYLPIKCA 209 >SPAC11D3.06 |||MatE family transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 455 Score = 25.0 bits (52), Expect = 7.0 Identities = 15/36 (41%), Positives = 18/36 (50%) Frame = +3 Query: 219 LYDIAPVTPGVAADLSHVNTPAKVSGHKGPEELSXA 326 L + APV G A S + V+G GP ELS A Sbjct: 12 LINSAPVILGYALQNSLQTSSVIVTGRLGPSELSVA 47 >SPCC18.03 |||shuttle craft like transcriptional regulator|Schizosaccharomyces pombe|chr 3|||Manual Length = 1077 Score = 24.6 bits (51), Expect = 9.2 Identities = 10/32 (31%), Positives = 14/32 (43%) Frame = +2 Query: 146 WCRRWYRPAFGPSTEAESSGDQAGFIRHSACD 241 WC + +P F + S GD G R C+ Sbjct: 260 WCGKQEKPEFVKNLVPHSCGDPCGKTRGQDCE 291 >SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces pombe|chr 2|||Manual Length = 937 Score = 24.6 bits (51), Expect = 9.2 Identities = 15/70 (21%), Positives = 31/70 (44%) Frame = +3 Query: 297 HKGPEELSXAIKDADVVVIPXGVPREPGMTRDDLFNTNASIVRDIALSIAXNAPKAIVAI 476 H E ++ ++ AD+VV G+P + D + D+ ++ +A K Sbjct: 192 HSKTESIADIVRTADIVVAAIGIPH---FVKADWLKKGV-VAIDVGINSIPDATKKSGYR 247 Query: 477 ITNPVNFYSA 506 +T ++F +A Sbjct: 248 LTGDIDFENA 257 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,099,747 Number of Sequences: 5004 Number of extensions: 41088 Number of successful extensions: 94 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 91 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 92 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 216376042 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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