BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= maV30003 (687 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC11C11.09c |rpl502|rpl5-2, rpl5b|60S ribosomal protein L5|Sch... 180 2e-46 SPAC3H5.12c |rpl501|rpl5-1, rpl5|60S ribosomal protein L5|Schizo... 178 5e-46 SPAC23G3.06 |||U3 snoRNP protein Nop58 |Schizosaccharomyces pomb... 29 0.48 SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|... 29 0.83 SPBC646.10c |||U3 snoRNP protein Nop56 |Schizosaccharomyces pomb... 29 0.83 SPAC140.04 |||conserved fungal protein|Schizosaccharomyces pombe... 29 0.83 SPAC22F3.08c |rok1||ATP-dependent RNA helicase Rok1 |Schizosacch... 28 1.1 SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 27 1.9 SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 27 2.5 SPBC25B2.10 |||Usp |Schizosaccharomyces pombe|chr 2|||Manual 27 2.5 SPAC29A4.04c |||pseudouridylate synthase |Schizosaccharomyces po... 27 3.4 SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces po... 26 4.4 SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos... 26 4.4 SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual 26 4.4 SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 26 5.9 SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyc... 26 5.9 SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 26 5.9 SPCC1827.06c |||aspartate semialdehyde dehydrogenase|Schizosacch... 25 7.8 SPAC17C9.11c |||zinc finger protein, zf-C2H2 type/UBA domain pro... 25 7.8 SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual 25 7.8 >SPBC11C11.09c |rpl502|rpl5-2, rpl5b|60S ribosomal protein L5|Schizosaccharomyces pombe|chr 2|||Manual Length = 294 Score = 180 bits (438), Expect = 2e-46 Identities = 92/215 (42%), Positives = 129/215 (60%), Gaps = 2/215 (0%) Frame = -2 Query: 686 HTVCAAYSHELPRYGVKVGLTNYAAAYSTGXXXXXXXXXXXXXXXXXXXXXXXXXDEYNV 507 + + A+S ELPRYG+K GL N+ AAY+TG + Sbjct: 73 YVLAHAHSSELPRYGIKWGLANWTAAYATGLLVARRALAKVGLADKYEGVTEPEGEFELT 132 Query: 506 EPVDNGPGAFRCYLDVGLARTTTGARVFGAMKGAVDGGLNVPHSIKRFPGYDAESKKFNA 327 E +++GP F+ +LDVGL RT+TG+RVFGAMKGA DGGL +PHS RFPG+D E+++ + Sbjct: 133 EAIEDGPRPFKVFLDVGLKRTSTGSRVFGAMKGASDGGLFIPHSPNRFPGFDIETEELDD 192 Query: 326 EVHRAHIFGLHVAEYMRSLEQDDEDSFKRQFSKYIKLGVTADAIEAIYKKAHEAIRADPS 147 E R +I+G HVAEYM L DDE+ +++QFS I G+ +D +E IY +A+ IR DPS Sbjct: 193 ETLRKYIYGGHVAEYMEMLIDDDEERYQKQFSGLIADGIESDQLEDIYAEAYAKIREDPS 252 Query: 146 HKK--KELKKDSVKQKRWNKRKLTLAERKNRIKQK 48 +K K+ + ++ +RKLT ERK R K Sbjct: 253 FQKSGKDAAAFKAESLKYTQRKLTAEERKERFNAK 287 >SPAC3H5.12c |rpl501|rpl5-1, rpl5|60S ribosomal protein L5|Schizosaccharomyces pombe|chr 1|||Manual Length = 294 Score = 178 bits (434), Expect = 5e-46 Identities = 92/215 (42%), Positives = 128/215 (59%), Gaps = 2/215 (0%) Frame = -2 Query: 686 HTVCAAYSHELPRYGVKVGLTNYAAAYSTGXXXXXXXXXXXXXXXXXXXXXXXXXDEYNV 507 + + A+S ELPRYG+K GL N+ AAY+TG + Sbjct: 73 YVLAHAHSSELPRYGIKWGLANWTAAYATGLLVARRALAKVGLADKYEGVTEPEGEFELT 132 Query: 506 EPVDNGPGAFRCYLDVGLARTTTGARVFGAMKGAVDGGLNVPHSIKRFPGYDAESKKFNA 327 E +++GP F+ +LDVGL RT+TG+RVFGAMKGA DGGL +PHS RFPG+D E+++ + Sbjct: 133 EAIEDGPRPFKVFLDVGLKRTSTGSRVFGAMKGASDGGLFIPHSPNRFPGFDIETEELDD 192 Query: 326 EVHRAHIFGLHVAEYMRSLEQDDEDSFKRQFSKYIKLGVTADAIEAIYKKAHEAIRADPS 147 E R +I+G HVAEYM L DDE+ +++QFS I G+ +D +E IY +A+ IR DPS Sbjct: 193 ETLRKYIYGGHVAEYMEMLIDDDEERYQKQFSGLIADGIESDQLEDIYAEAYAKIREDPS 252 Query: 146 HKK--KELKKDSVKQKRWNKRKLTLAERKNRIKQK 48 +K K+ + + +RKLT ERK R K Sbjct: 253 FQKSGKDAAAFKAESLKHTQRKLTAEERKERFNAK 287 >SPAC23G3.06 |||U3 snoRNP protein Nop58 |Schizosaccharomyces pombe|chr 1|||Manual Length = 508 Score = 29.5 bits (63), Expect = 0.48 Identities = 19/53 (35%), Positives = 27/53 (50%) Frame = -2 Query: 197 IEAIYKKAHEAIRADPSHKKKELKKDSVKQKRWNKRKLTLAERKNRIKQKKAS 39 IE KK+ + AD KKE K+ K+ + +KRK +E K+KK S Sbjct: 451 IEKKKKKSSKLKEADGESSKKEKKEKKDKKHKKSKRKSEESEDGESPKKKKKS 503 >SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1184 Score = 28.7 bits (61), Expect = 0.83 Identities = 17/59 (28%), Positives = 35/59 (59%), Gaps = 2/59 (3%) Frame = -2 Query: 182 KKAHEAIRADPSHKKKELKKDSVKQKRWNKR--KLTLAERKNRIKQKKASFIKRLQDQA 12 + A E R + + + K LKK + ++KR K+ K AE K R+++++A + K++ ++ Sbjct: 708 RAAQERERKEQAKEAKRLKKLAKEEKRLKKKEEKARKAEEK-RLQKERAKYAKQMSRES 765 >SPBC646.10c |||U3 snoRNP protein Nop56 |Schizosaccharomyces pombe|chr 2|||Manual Length = 497 Score = 28.7 bits (61), Expect = 0.83 Identities = 17/45 (37%), Positives = 24/45 (53%), Gaps = 1/45 (2%) Frame = -2 Query: 170 EAIRADPSHKKKELKKDSVKQKRWNKRKL-TLAERKNRIKQKKAS 39 E I PS K+K+ KK+ K+K KR +E + K+KK S Sbjct: 452 ETISEKPSKKEKKDKKEKKKEKSKKKRSADDASEEVKKSKKKKKS 496 >SPAC140.04 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 295 Score = 28.7 bits (61), Expect = 0.83 Identities = 15/56 (26%), Positives = 29/56 (51%) Frame = -2 Query: 182 KKAHEAIRADPSHKKKELKKDSVKQKRWNKRKLTLAERKNRIKQKKASFIKRLQDQ 15 KK +E + SH+K K KRKL + +++ K+ +++ IK+ +D+ Sbjct: 27 KKRYEDEKRKISHQKYTDKLLEANDNETTKRKLLVKQKRKSNKEFQSNIIKKRKDE 82 >SPAC22F3.08c |rok1||ATP-dependent RNA helicase Rok1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 481 Score = 28.3 bits (60), Expect = 1.1 Identities = 22/79 (27%), Positives = 38/79 (48%) Frame = -2 Query: 248 FKRQFSKYIKLGVTADAIEAIYKKAHEAIRADPSHKKKELKKDSVKQKRWNKRKLTLAER 69 F ++ +YIKL A + + + + A P KE+KK K KR T A Sbjct: 392 FTKEDGEYIKL--IAGVMRSSGCEVPNWVMALPK-PSKEMKKKLKKSPPKRKRITTRASY 448 Query: 68 KNRIKQKKASFIKRLQDQA 12 + +Q+K +IK+++ +A Sbjct: 449 DRQKEQRKKEYIKKVKKEA 467 >SPCC162.08c |nup211||nuclear pore complex associated protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1837 Score = 27.5 bits (58), Expect = 1.9 Identities = 13/54 (24%), Positives = 29/54 (53%) Frame = -2 Query: 266 QDDEDSFKRQFSKYIKLGVTADAIEAIYKKAHEAIRADPSHKKKELKKDSVKQK 105 +++++S ++ +K ++ V +KK +E IR+D LK+D K++ Sbjct: 1023 KEEKESSSKELAKQLEDAVREKDSALSFKKDYEKIRSDADRVITSLKEDIEKER 1076 >SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 27.1 bits (57), Expect = 2.5 Identities = 17/60 (28%), Positives = 34/60 (56%) Frame = -2 Query: 194 EAIYKKAHEAIRADPSHKKKELKKDSVKQKRWNKRKLTLAERKNRIKQKKASFIKRLQDQ 15 EA K+ + ++ + K++EL++ QKR K+K E+K + +Q++A K ++Q Sbjct: 636 EARKKREEQRLKREQEKKQQELER----QKREEKQKQKEREKKLKKQQQEADREKMAREQ 691 >SPBC25B2.10 |||Usp |Schizosaccharomyces pombe|chr 2|||Manual Length = 307 Score = 27.1 bits (57), Expect = 2.5 Identities = 11/31 (35%), Positives = 20/31 (64%) Frame = +2 Query: 572 EAVFVLIADQLNMLQHNLSDQPSHHNVATHV 664 EAV + + D + L +LSD+ S+ ++A H+ Sbjct: 169 EAVVLRVIDPSSKLAEDLSDEQSYRSLAEHI 199 >SPAC29A4.04c |||pseudouridylate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 474 Score = 26.6 bits (56), Expect = 3.4 Identities = 13/38 (34%), Positives = 22/38 (57%) Frame = -2 Query: 200 AIEAIYKKAHEAIRADPSHKKKELKKDSVKQKRWNKRK 87 ++E+ K EA + + +KKE KK+ K+K+ K K Sbjct: 430 SVESSEKDEDEAAKKEEKRRKKEAKKEK-KEKKEKKEK 466 >SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 284 Score = 26.2 bits (55), Expect = 4.4 Identities = 22/79 (27%), Positives = 36/79 (45%), Gaps = 2/79 (2%) Frame = -2 Query: 269 EQDDEDSFKRQFSKYIKLGVTADA--IEAIYKKAHEAIRADPSHKKKELKKDSVKQKRWN 96 E+ + S K++ K G D +E I KK + HKKK+ + S K+K + Sbjct: 166 EKRKKHSSKKKSKKKTSTGSALDPKKLEKITKKKKK------EHKKKDKESSSKKRKSGS 219 Query: 95 KRKLTLAERKNRIKQKKAS 39 K ++K ++K K S Sbjct: 220 SDKEEKKKKKIKLKDKPES 238 >SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizosaccharomyces pombe|chr 2|||Manual Length = 932 Score = 26.2 bits (55), Expect = 4.4 Identities = 18/57 (31%), Positives = 27/57 (47%), Gaps = 1/57 (1%) Frame = -2 Query: 194 EAIYKKAHEAIRADPSHKKKELKKDSVKQKR-WNKRKLTLAERKNRIKQKKASFIKR 27 EAI + EA RA +EL + QKR +R E K R +++ A ++R Sbjct: 788 EAIREAEEEAARAAEEEANRELHEQEEAQKRAIEERTRAAREAKEREQREMAEKLER 844 >SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 26.2 bits (55), Expect = 4.4 Identities = 15/28 (53%), Positives = 19/28 (67%) Frame = -2 Query: 209 TADAIEAIYKKAHEAIRADPSHKKKELK 126 TA IEA+YKKA+ I+ D +H K LK Sbjct: 174 TAQLIEAVYKKAN--IK-DAAHDSKNLK 198 >SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1049 Score = 25.8 bits (54), Expect = 5.9 Identities = 23/101 (22%), Positives = 39/101 (38%) Frame = -2 Query: 335 FNAEVHRAHIFGLHVAEYMRSLEQDDEDSFKRQFSKYIKLGVTADAIEAIYKKAHEAIRA 156 FNA R+ + A+ D E F + G A+ +IY+ + E IR+ Sbjct: 363 FNASGERSESANVETAQVW----DDREQYFFYEVFPNFNEGSIAEMKSSIYESSQEGIRS 418 Query: 155 DPSHKKKELKKDSVKQKRWNKRKLTLAERKNRIKQKKASFI 33 + KKE D +K + ++ R + K S + Sbjct: 419 SSENNKKE---DDLKDSTGDLNTTQVSSRVDNFLLKLPSMV 456 >SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 682 Score = 25.8 bits (54), Expect = 5.9 Identities = 16/46 (34%), Positives = 28/46 (60%), Gaps = 2/46 (4%) Frame = -2 Query: 137 KELKKDSVKQKRWNKRKLTLAER--KNRIKQKKASFIKRLQDQAEA 6 +E + DS + ++ + + AE+ KNR +KKAS ++ LQ+ EA Sbjct: 553 REDENDSEQLDHFDMKSILKAEKFKKNRKLKKKASNLEGLQEGFEA 598 >SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|||Manual Length = 207 Score = 25.8 bits (54), Expect = 5.9 Identities = 18/60 (30%), Positives = 30/60 (50%), Gaps = 2/60 (3%) Frame = -2 Query: 182 KKAHEAIRADPSHKKKE--LKKDSVKQKRWNKRKLTLAERKNRIKQKKASFIKRLQDQAE 9 KK E A S K+ E KKD+ + ++W+K T + + K+K A K +++ E Sbjct: 5 KKRAEKAEAAKSRKQDEEKKKKDAEEDEKWSKGVKTNKKEQEAEKRKAALERKAERERLE 64 >SPCC1827.06c |||aspartate semialdehyde dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual Length = 357 Score = 25.4 bits (53), Expect = 7.8 Identities = 12/39 (30%), Positives = 22/39 (56%) Frame = -3 Query: 583 EDCFKDLDLTLYTLAQQMSQVMNTMLNLSTMDQEHLDVI 467 E F+D +L + + A+ + L + T++ +HLDVI Sbjct: 93 EKSFRDANLVIVSNAKNYRREPTVPLVVPTVNTDHLDVI 131 >SPAC17C9.11c |||zinc finger protein, zf-C2H2 type/UBA domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 240 Score = 25.4 bits (53), Expect = 7.8 Identities = 19/67 (28%), Positives = 37/67 (55%), Gaps = 9/67 (13%) Frame = -2 Query: 185 YKKAHEAIRA-DPSHKKKELK--KDSVKQKRWNKRKLTLAERKN--RIKQKK----ASFI 33 +++ E I+ P K+ ++ ++ K+K+ +R L L E+K +I QK A + Sbjct: 30 FEETEEEIKKRSPEELKQAIEALREKAKEKKEKERILALEEKKTNYKILQKSNDETAQAM 89 Query: 32 KRLQDQA 12 +++QDQA Sbjct: 90 RKMQDQA 96 >SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual Length = 978 Score = 25.4 bits (53), Expect = 7.8 Identities = 10/24 (41%), Positives = 17/24 (70%) Frame = -2 Query: 290 AEYMRSLEQDDEDSFKRQFSKYIK 219 +EY+++L D SFK+ F K+I+ Sbjct: 948 SEYLQALSNLDSRSFKQFFQKFIQ 971 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,600,252 Number of Sequences: 5004 Number of extensions: 49377 Number of successful extensions: 248 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 223 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 241 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 317927284 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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