Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS31000
(500 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC17G9.07 |rps2402|rps24-2|40S ribosomal protein S24|Schizosac... 100 2e-22
SPAC17G6.06 |rps2401|rps24-1, rps24|40S ribosomal protein S24|Sc... 94 1e-20
SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|S... 28 0.91
SPAC22A12.11 |dak1||dihydroxyacetone kinase Dak1|Schizosaccharom... 27 1.2
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 26 3.7
SPBC25B2.04c |||mitochondrial ribosome assembly protein|Schizosa... 25 4.8
SPAC1952.02 |||ribosome biogenesis protein|Schizosaccharomyces p... 25 8.4
SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr ... 25 8.4
SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces pomb... 25 8.4
SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22... 25 8.4
SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schi... 25 8.4
SPBC32H8.10 |cdk9||cyclin-dependent protein kinase Cdk9 |Schizos... 25 8.4
SPBC776.03 |||homoserine dehydrogenase |Schizosaccharomyces pomb... 25 8.4
>SPBC17G9.07 |rps2402|rps24-2|40S ribosomal protein
S24|Schizosaccharomyces pombe|chr 2|||Manual
Length = 134
Score = 99.5 bits (237), Expect = 2e-22
Identities = 48/70 (68%), Positives = 55/70 (78%)
Frame = +3
Query: 45 MSEGTATIRTRKFMTNRLLARKQMVCDVLHPGKPTVSKTEIREKLAKMYKVTPDVVFVFG 224
MSE TIRTRKFMTNRLL RKQMV D+LHPGK +SK EIREKLA+MYK + V FG
Sbjct: 1 MSEAV-TIRTRKFMTNRLLQRKQMVVDILHPGKANLSKNEIREKLAQMYKTDSECVQAFG 59
Query: 225 FKTNFGGGKS 254
+T+FGGG+S
Sbjct: 60 LRTHFGGGRS 69
Score = 60.5 bits (140), Expect = 1e-10
Identities = 31/52 (59%), Positives = 38/52 (73%), Gaps = 1/52 (1%)
Frame = +2
Query: 254 TGFALIYDTLDLAKKFEPKHRLARHGLYEK-KRPTRKQRKERKNRMKKVRGT 406
TGFALIYD+ + KKFEP +RL R G E ++ R+QRK+RKNR KKV GT
Sbjct: 70 TGFALIYDSTESMKKFEPHYRLVRVGQAEPIQKVARQQRKQRKNRGKKVFGT 121
>SPAC17G6.06 |rps2401|rps24-1, rps24|40S ribosomal protein
S24|Schizosaccharomyces pombe|chr 1|||Manual
Length = 134
Score = 93.9 bits (223), Expect = 1e-20
Identities = 43/70 (61%), Positives = 53/70 (75%)
Frame = +3
Query: 45 MSEGTATIRTRKFMTNRLLARKQMVCDVLHPGKPTVSKTEIREKLAKMYKVTPDVVFVFG 224
MSE TIRTRKFMTNRLL RKQMV D+LHPGK +SK ++REKL +MYK V FG
Sbjct: 1 MSEAV-TIRTRKFMTNRLLQRKQMVVDILHPGKANISKNDLREKLGQMYKTDASAVQAFG 59
Query: 225 FKTNFGGGKS 254
+T++GGG++
Sbjct: 60 LRTHYGGGRT 69
Score = 60.9 bits (141), Expect = 1e-10
Identities = 31/54 (57%), Positives = 39/54 (72%), Gaps = 1/54 (1%)
Frame = +2
Query: 248 QVTGFALIYDTLDLAKKFEPKHRLARHGLYEK-KRPTRKQRKERKNRMKKVRGT 406
+ TGFALIYD ++ KKFEP +RL R G E ++ R+QRK+RKNR KKV GT
Sbjct: 68 RTTGFALIYDDVEAMKKFEPHYRLVRVGHAEPIQKVARQQRKQRKNRGKKVFGT 121
>SPCC417.06c |ppk35|mug27|serine/threonine protein kinase
Ppk35|Schizosaccharomyces pombe|chr 3|||Manual
Length = 624
Score = 27.9 bits (59), Expect = 0.91
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = +2
Query: 296 KFEPKHRLARHGLYEKKRPTRKQRKERKNRMKK 394
K+ P R G + +KR RK++ E+KN K
Sbjct: 539 KYRPNARKPLVGRHREKRQLRKEKPEKKNNSTK 571
>SPAC22A12.11 |dak1||dihydroxyacetone kinase
Dak1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 580
Score = 27.5 bits (58), Expect = 1.2
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +3
Query: 162 EIREKLAKMYKVTPDVVFVFGFKTNFGG 245
+++E LAK YK+ P +F F T+ G
Sbjct: 286 KVKEALAKEYKINPVRIFAGPFTTSLNG 313
>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1828
Score = 25.8 bits (54), Expect = 3.7
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = -1
Query: 77 ASANSRCSFTHFELFSII 24
+S SRCSFT FE+ SI+
Sbjct: 777 SSVISRCSFTDFEINSIL 794
>SPBC25B2.04c |||mitochondrial ribosome assembly
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 328
Score = 25.4 bits (53), Expect = 4.8
Identities = 23/91 (25%), Positives = 37/91 (40%), Gaps = 1/91 (1%)
Frame = -2
Query: 316 PVLGLELLGQIECVVDQSESSDLPPPKFVLKPNTNTTSGVTLYILASFSRISVLLTVGFP 137
P ELL I +VDQ+ ++ FV PNT +S + + + + +P
Sbjct: 123 PYFAKELLRLIRTLVDQASANGRVYVYFVGMPNTGKSSILNSLRNVALRKSKSAIVGNYP 182
Query: 136 G-CKTSQTICLRANNLLVMNLRVRIVAVPSL 47
G K I N++ V L + PS+
Sbjct: 183 GVTKRISEIVRLFNDMDVYMLDTPGIMTPSI 213
>SPAC1952.02 |||ribosome biogenesis protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 202
Score = 24.6 bits (51), Expect = 8.4
Identities = 9/35 (25%), Positives = 21/35 (60%)
Frame = +2
Query: 290 AKKFEPKHRLARHGLYEKKRPTRKQRKERKNRMKK 394
+KK + + + +K +K++KE+K+++KK
Sbjct: 125 SKKVKVRKTSGKESSKREKSKKKKEKKEKKDKLKK 159
>SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1854
Score = 24.6 bits (51), Expect = 8.4
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = -2
Query: 244 PPKFVLKPNTNTTSGVTLYILASFS 170
PPK LKP+ +T+ ++ Y LA S
Sbjct: 818 PPKEPLKPSLDTSPELSKYSLAKLS 842
>SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 440
Score = 24.6 bits (51), Expect = 8.4
Identities = 17/39 (43%), Positives = 20/39 (51%), Gaps = 5/39 (12%)
Frame = +2
Query: 296 KFEPKHRLARHGLYEKKRPT-----RKQRKERKNRMKKV 397
+F KH L RHG EKKR R+Q + R KKV
Sbjct: 58 EFGTKH-LRRHGRIEKKRRNWKGLFRRQSNWKDGRCKKV 95
>SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 332
Score = 24.6 bits (51), Expect = 8.4
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = -2
Query: 172 SRISVLLTVGFPGCKTSQTICLRANNL 92
SRI + ++G K Q++CLR N +
Sbjct: 48 SRIQSMASLGLERFKNLQSLCLRQNQI 74
>SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit
Rpt2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 448
Score = 24.6 bits (51), Expect = 8.4
Identities = 8/23 (34%), Positives = 17/23 (73%)
Frame = +2
Query: 338 EKKRPTRKQRKERKNRMKKVRGT 406
E+ +P ++ +E +NR+ ++RGT
Sbjct: 90 ERLKPQDERTQEERNRVDEIRGT 112
>SPBC32H8.10 |cdk9||cyclin-dependent protein kinase Cdk9
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 591
Score = 24.6 bits (51), Expect = 8.4
Identities = 11/39 (28%), Positives = 21/39 (53%)
Frame = +2
Query: 53 RNSDYSHSQVHDQQIVGAQADGLRCFTSRKTNRQQDRDP 169
+NS++S + +Q + ADG R + + Q+ R+P
Sbjct: 449 KNSEFSETNAMNQTSNHSHADGQRYYRPEQDRSQRLRNP 487
>SPBC776.03 |||homoserine dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 376
Score = 24.6 bits (51), Expect = 8.4
Identities = 14/48 (29%), Positives = 27/48 (56%), Gaps = 1/48 (2%)
Frame = -2
Query: 385 SVLTFFTL-FARGPLLLVQAVAG*PVLGLELLGQIECVVDQSESSDLP 245
++++F T + PL+++ A AG V +LG + ++ Q +SD P
Sbjct: 327 NIISFTTKRYHTRPLVVIGAGAGAAVTAAGVLGDMIKIMSQVRASDAP 374
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,979,850
Number of Sequences: 5004
Number of extensions: 39557
Number of successful extensions: 131
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 121
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 129
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 198176188
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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