SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS30994
         (789 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde reduc...    65   1e-11
SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism...    62   9e-11
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy...    60   3e-10
SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyc...    49   9e-07
SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc...    41   2e-04
SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomy...    41   2e-04
SPCC965.06 |||potassium channel subunit |Schizosaccharomyces pom...    35   0.012
SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase YakC|Schizo...    31   0.19 
SPAC25A8.02 |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    28   1.3  
SPCC1795.12c |||sequence orphan|Schizosaccharomyces pombe|chr 3|...    28   1.8  
SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyce...    28   1.8  
SPAC1093.01 ||SPAC12B10.18|PPR repeat protein|Schizosaccharomyce...    27   4.0  
SPBC409.12c |||nuclear telomere cap complex subunit Stn1|Schizos...    26   5.4  
SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomy...    26   5.4  
SPAC977.14c |||aldo/keto reductase, unknown biological role|Schi...    26   7.1  
SPBC1709.15c |cft2||cleavage factor two Cft2/polyadenylation fac...    26   7.1  
SPBC83.08 |||AAA family ATPase Rvb2 |Schizosaccharomyces pombe|c...    26   7.1  
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni...    25   9.4  
SPCC14G10.04 |||sequence orphan|Schizosaccharomyces pombe|chr 3|...    25   9.4  
SPAC637.12c |mst1||histone acetyltransferase Mst1|Schizosaccharo...    25   9.4  

>SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde
           reductase AKR3C2|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 284

 Score = 64.9 bits (151), Expect = 1e-11
 Identities = 32/66 (48%), Positives = 40/66 (60%), Gaps = 2/66 (3%)
 Frame = +2

Query: 506 GIVKGIGLSNFNKQQLERVLKEGTIKPDAIQIEVHLQ--NVQKEMVEFCQSEGVVVMGYS 679
           G+V  +G+SNF    LE +LK  TI P   QIE H Q     K +VEFCQS+G++V GY 
Sbjct: 135 GLVHSVGVSNFRIPDLEELLKTSTITPRVNQIEFHPQVYKAAKPLVEFCQSKGIIVEGYG 194

Query: 680 PFGSLV 697
           P   LV
Sbjct: 195 PLSPLV 200



 Score = 58.8 bits (136), Expect = 8e-10
 Identities = 31/85 (36%), Positives = 48/85 (56%)
 Frame = +3

Query: 255 EAVKMKVEEGVVKREDIFITTKLWNTQHRRTEVAQAINDSLKKLGLDYIDLYLMHWPIGL 434
           E V + ++E  V R  +FIT+K+    H    + +A+N+SL+KLG DY+DLYL+H PI  
Sbjct: 58  EEVGVALKEANVPRSKLFITSKV---MHNVDNIPEALNESLRKLGTDYLDLYLLHSPI-- 112

Query: 435 NADYSHSDVDFMETWRGLEDAQRMG 509
              +    +   E W+ +E A   G
Sbjct: 113 --PFYEKKIPISEGWKAMETALGTG 135



 Score = 37.5 bits (83), Expect = 0.002
 Identities = 19/53 (35%), Positives = 30/53 (56%)
 Frame = +1

Query: 97  MPAIGLGTYLGFDQNGVVKSKDKQLRDVVMRAIDVGYRHFDTASVYETEQEIG 255
           +PA G+GT L   + G +   ++ + D V  A+  G+ H D A VY  E+E+G
Sbjct: 12  VPAYGVGTALFKKEKGEI---NRTIVDSVKNALAAGFIHIDCAEVYGNEEEVG 61


>SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 325

 Score = 62.1 bits (144), Expect = 9e-11
 Identities = 32/90 (35%), Positives = 46/90 (51%), Gaps = 11/90 (12%)
 Frame = +3

Query: 273 VEEGVVKREDIFITTKLWNTQHRRTEVAQAINDSLKKLGLDYIDLYLMHWPIGL------ 434
           + E  V R DI++T+KLW   HR   V  A+  +L+ L L+YID YL+HWP  L      
Sbjct: 69  IRESGVPRTDIWVTSKLWCNAHRAGLVPLALEKTLQDLNLEYIDAYLIHWPFALLSGPEE 128

Query: 435 -----NADYSHSDVDFMETWRGLEDAQRMG 509
                  +  + DV   ETW+ +E+    G
Sbjct: 129 LPRNEKGELIYEDVPIEETWQAMEELLETG 158



 Score = 48.8 bits (111), Expect = 9e-07
 Identities = 31/89 (34%), Positives = 45/89 (50%)
 Frame = +2

Query: 506 GIVKGIGLSNFNKQQLERVLKEGTIKPDAIQIEVHLQNVQKEMVEFCQSEGVVVMGYSPF 685
           G V+ IG+SNFN + L+RVLK   +KP   Q+E+H    Q E +E  +   + V  YSP 
Sbjct: 158 GKVRYIGISNFNNEYLDRVLKIAKVKPTIHQMELHPYLPQTEYLEKHKKLQIHVSAYSPL 217

Query: 686 GSLVARHGSTVEGPKIDDPVLSSIAQNTG 772
            +    + S +    I+   L  IA   G
Sbjct: 218 ANQNDAYNSDI-SKLIEHKTLVDIANARG 245



 Score = 44.8 bits (101), Expect = 1e-05
 Identities = 23/58 (39%), Positives = 34/58 (58%)
 Frame = +1

Query: 85  NGRDMPAIGLGTYLGFDQNGVVKSKDKQLRDVVMRAIDVGYRHFDTASVYETEQEIGK 258
           NG  +P+IGLGT+         +S   + ++ V  A+  GYRH DTA +Y  E+EIG+
Sbjct: 19  NGDKIPSIGLGTW---------RSGKDETKNAVCAALKAGYRHIDTAHIYGNEKEIGE 67


>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 321

 Score = 60.5 bits (140), Expect = 3e-10
 Identities = 37/90 (41%), Positives = 51/90 (56%), Gaps = 1/90 (1%)
 Frame = +2

Query: 506 GIVKGIGLSNFNKQQLERVLKEGTIKPDAIQIEVHLQNVQKEMVEFCQSEGVVVMGYSPF 685
           G V+ IGLSNFN   LER+LK   +KP   Q+E+H    Q E VE  +  G+ V  YSPF
Sbjct: 159 GKVRHIGLSNFNDTNLERILKVAKVKPAVHQMELHPFLPQTEFVEKHKKLGIHVTAYSPF 218

Query: 686 GSLVARHGSTVEGPK-IDDPVLSSIAQNTG 772
           G+    + S +  PK I+   +  IA++ G
Sbjct: 219 GNQNTIYESKI--PKLIEHETIQKIAKSKG 246



 Score = 59.3 bits (137), Expect = 6e-10
 Identities = 24/52 (46%), Positives = 36/52 (69%)
 Frame = +3

Query: 273 VEEGVVKREDIFITTKLWNTQHRRTEVAQAINDSLKKLGLDYIDLYLMHWPI 428
           ++E  V R+DI++T+KLW   H    V +A+  +LK L LDY+D YL+HWP+
Sbjct: 70  IKESGVPRKDIWVTSKLWCNAHAPEAVPKALEKTLKDLKLDYLDEYLIHWPV 121



 Score = 42.3 bits (95), Expect = 8e-05
 Identities = 20/57 (35%), Positives = 32/57 (56%)
 Frame = +1

Query: 85  NGRDMPAIGLGTYLGFDQNGVVKSKDKQLRDVVMRAIDVGYRHFDTASVYETEQEIG 255
           +G  +P +GLGT+         +S+  Q ++ V  A+  GYRH D A++Y  E E+G
Sbjct: 20  DGSKIPGLGLGTW---------RSEPNQTKNAVKTALQYGYRHIDAAAIYGNEDEVG 67


>SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 275

 Score = 48.8 bits (111), Expect = 9e-07
 Identities = 32/88 (36%), Positives = 50/88 (56%)
 Frame = +3

Query: 258 AVKMKVEEGVVKREDIFITTKLWNTQHRRTEVAQAINDSLKKLGLDYIDLYLMHWPIGLN 437
           A+   +EE   KREDI+ T+KL +    ++ ++ +I+ S+K  GL YIDL+L+H P G  
Sbjct: 59  AILKFMEETGTKREDIWFTSKLNDLSGYKSTLS-SIDASVKACGLGYIDLFLLHSPYG-- 115

Query: 438 ADYSHSDVDFMETWRGLEDAQRMG*LKA 521
                   D +E+W+ LE     G L+A
Sbjct: 116 --------DRIESWKALEKGVEEGKLRA 135



 Score = 42.7 bits (96), Expect = 6e-05
 Identities = 20/60 (33%), Positives = 37/60 (61%), Gaps = 1/60 (1%)
 Frame = +2

Query: 506 GIVKGIGLSNFNKQQLERVL-KEGTIKPDAIQIEVHLQNVQKEMVEFCQSEGVVVMGYSP 682
           G ++ IG+SNF    ++ +L     I P   QIE+H    Q+++V++C+S+G+ +  Y+P
Sbjct: 131 GKLRAIGVSNFGPHHIQELLDSHPKIIPCVNQIELHPFCSQQKVVDYCESKGIQLAAYAP 190



 Score = 36.7 bits (81), Expect = 0.004
 Identities = 21/62 (33%), Positives = 32/62 (51%)
 Frame = +1

Query: 73  LKMNNGRDMPAIGLGTYLGFDQNGVVKSKDKQLRDVVMRAIDVGYRHFDTASVYETEQEI 252
           +K+NNG   P    G+Y+      V ++K     D V  A+  GYRH D+A +Y  E + 
Sbjct: 6   VKLNNGLKCPQFAYGSYM------VNRTK---CFDSVYAALQCGYRHIDSAQMYHNEADC 56

Query: 253 GK 258
           G+
Sbjct: 57  GR 58


>SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 283

 Score = 41.1 bits (92), Expect = 2e-04
 Identities = 24/69 (34%), Positives = 35/69 (50%)
 Frame = +1

Query: 52  STMEVPSLKMNNGRDMPAIGLGTYLGFDQNGVVKSKDKQLRDVVMRAIDVGYRHFDTASV 231
           S  E  ++ + NG  +P IG G ++          K  +   +V +A+D GYRH DTA+V
Sbjct: 4   SQTESTTVTLTNGMVIPRIGFGAFM---------LKYNECYGLVTQALDSGYRHIDTAAV 54

Query: 232 YETEQEIGK 258
           Y  E   GK
Sbjct: 55  YGNEDICGK 63



 Score = 39.9 bits (89), Expect = 4e-04
 Identities = 18/61 (29%), Positives = 34/61 (55%), Gaps = 1/61 (1%)
 Frame = +2

Query: 503 NGIVKGIGLSNFNKQQLERVLKEGT-IKPDAIQIEVHLQNVQKEMVEFCQSEGVVVMGYS 679
           +G ++ +G+SN+  + L+ +        P   QIE+H    Q ++V++CQS  + +  YS
Sbjct: 137 SGDIRSVGVSNYGVKHLQELYASNPKFYPCVNQIELHPFLSQDDIVKYCQSHDIAIEAYS 196

Query: 680 P 682
           P
Sbjct: 197 P 197



 Score = 38.7 bits (86), Expect = 0.001
 Identities = 25/52 (48%), Positives = 31/52 (59%)
 Frame = +3

Query: 276 EEGVVKREDIFITTKLWNTQHRRTEVAQAINDSLKKLGLDYIDLYLMHWPIG 431
           E+  VKR DIF+T+KL N     +  A AI  SL  LG  YIDL+L+  P G
Sbjct: 70  EKNNVKRTDIFLTSKLANCSDYYSTRA-AIRSSLHHLG-TYIDLFLIQSPAG 119


>SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 276

 Score = 40.7 bits (91), Expect = 2e-04
 Identities = 18/59 (30%), Positives = 31/59 (52%)
 Frame = +2

Query: 506 GIVKGIGLSNFNKQQLERVLKEGTIKPDAIQIEVHLQNVQKEMVEFCQSEGVVVMGYSP 682
           G +  IG+SN+N   LE ++  G   P   Q+E    N +   + +C + G++V  +SP
Sbjct: 132 GRINKIGVSNYNIHHLEEIISLGLPLPAINQVEFSAFNNRPTFLSYCFNHGILVQAFSP 190



 Score = 33.5 bits (73), Expect = 0.035
 Identities = 16/50 (32%), Positives = 29/50 (58%), Gaps = 3/50 (6%)
 Frame = +3

Query: 288 VKREDIFITTKLWNTQHRRTEVAQAINDSLKKLGLDYIDLYLMH---WPI 428
           +KREDI + TK+ ++        +A+  SL++ G   +D+ L+H   WP+
Sbjct: 67  IKREDITLITKIPDSLQGFERTWKAVEQSLRRTGRPKLDVVLIHSPKWPV 116


>SPCC965.06 |||potassium channel subunit |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 344

 Score = 35.1 bits (77), Expect = 0.012
 Identities = 17/38 (44%), Positives = 24/38 (63%), Gaps = 1/38 (2%)
 Frame = +3

Query: 315 TKLWNTQH-RRTEVAQAINDSLKKLGLDYIDLYLMHWP 425
           TKL NT    R  + + +N SLK+LGL Y+D+ + H P
Sbjct: 102 TKLPNTTGLSRKHIIEGLNASLKRLGLPYVDVIMAHRP 139



 Score = 25.4 bits (53), Expect = 9.4
 Identities = 15/50 (30%), Positives = 24/50 (48%)
 Frame = +1

Query: 85  NGRDMPAIGLGTYLGFDQNGVVKSKDKQLRDVVMRAIDVGYRHFDTASVY 234
           +G  + A  LG +L +   G      K   + + +A D+G   FDTA +Y
Sbjct: 21  SGLKVSAFSLGGWLTYGNEGYDVEHTK---NCLKQAWDLGINTFDTAEIY 67


>SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase
           YakC|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 340

 Score = 31.1 bits (67), Expect = 0.19
 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 10/53 (18%)
 Frame = +3

Query: 291 KREDIFITTKLWNTQHRRTE----------VAQAINDSLKKLGLDYIDLYLMH 419
           +R++IF+ TK    ++  T           + +A++ SLK+LG+D IDLY +H
Sbjct: 74  RRKEIFLATKFGYEKNPETGELSLNNEPDYIEKALDLSLKRLGIDCIDLYYVH 126


>SPAC25A8.02 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 390

 Score = 28.3 bits (60), Expect = 1.3
 Identities = 13/37 (35%), Positives = 24/37 (64%), Gaps = 1/37 (2%)
 Frame = +3

Query: 315 TKLWNTQHRRTEVAQ-AINDSLKKLGLDYIDLYLMHW 422
           +K + T +RR+E+ Q  +++  KKL    +D+Y +HW
Sbjct: 125 SKEYLTAYRRSELLQNKLHEYQKKLITRVLDMYQLHW 161


>SPCC1795.12c |||sequence orphan|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 112

 Score = 27.9 bits (59), Expect = 1.8
 Identities = 12/30 (40%), Positives = 18/30 (60%)
 Frame = -3

Query: 325 HSLVVIKISSLLTTPSSTFIFTASQFLVQF 236
           H L+V ++  + T+    FIFT S FL+ F
Sbjct: 72  HMLLVQRLKRVSTSSRRLFIFTLSMFLIAF 101


>SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 2310

 Score = 27.9 bits (59), Expect = 1.8
 Identities = 16/74 (21%), Positives = 35/74 (47%)
 Frame = +2

Query: 533  NFNKQQLERVLKEGTIKPDAIQIEVHLQNVQKEMVEFCQSEGVVVMGYSPFGSLVARHGS 712
            +F+   L  V+K G +    +++   L  V K ++E+ Q++   ++        +A HG+
Sbjct: 1429 SFDIVSLVSVIKCGQLLSSKLRLGPLLTTVIKLVIEYSQAKHAAIILKDASNYTLAAHGN 1488

Query: 713  TVEGPKIDDPVLSS 754
              +    + PV+ S
Sbjct: 1489 VEKAESFEPPVILS 1502


>SPAC1093.01 ||SPAC12B10.18|PPR repeat protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1261

 Score = 26.6 bits (56), Expect = 4.0
 Identities = 9/13 (69%), Positives = 11/13 (84%)
 Frame = -2

Query: 272 LHLHGFPISCSVS 234
           LHLH +P+ CSVS
Sbjct: 535 LHLHAYPVICSVS 547


>SPBC409.12c |||nuclear telomere cap complex subunit
           Stn1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 325

 Score = 26.2 bits (55), Expect = 5.4
 Identities = 9/25 (36%), Positives = 16/25 (64%)
 Frame = +2

Query: 605 VHLQNVQKEMVEFCQSEGVVVMGYS 679
           VH+ NV+ E+VE C    ++ + +S
Sbjct: 208 VHIFNVENEIVENCDGNHILALNFS 232


>SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 874

 Score = 26.2 bits (55), Expect = 5.4
 Identities = 9/30 (30%), Positives = 19/30 (63%)
 Frame = +3

Query: 315 TKLWNTQHRRTEVAQAINDSLKKLGLDYID 404
           TK+WN  H+  E  + ++D++++  L  +D
Sbjct: 680 TKIWNCVHQLLEREKKMSDAMRQTKLTIVD 709


>SPAC977.14c |||aldo/keto reductase, unknown biological
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 351

 Score = 25.8 bits (54), Expect = 7.1
 Identities = 13/40 (32%), Positives = 23/40 (57%), Gaps = 3/40 (7%)
 Frame = +1

Query: 148 VKSKDKQLRDVVMRAIDVGYRHFDTASVYE---TEQEIGK 258
           V   ++++  ++  A D G R FDTA+ Y    +E+ +GK
Sbjct: 39  VLEDEEEVFKIMKAAYDAGIRTFDTANCYSAGVSEELVGK 78


>SPBC1709.15c |cft2||cleavage factor two Cft2/polyadenylation factor
           CPSF-73 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 797

 Score = 25.8 bits (54), Expect = 7.1
 Identities = 17/46 (36%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
 Frame = -1

Query: 558 LSNCCLLKLD-SPMPLTIPFAEHLPILARSP*SLRPNDYNRHLIQW 424
           L  CC+L    S     +PF    PIL  SP S +  DY + +I+W
Sbjct: 236 LELCCILDNHWSASQPPLPF----PILFLSPTSTKTIDYAKSMIEW 277


>SPBC83.08 |||AAA family ATPase Rvb2 |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 465

 Score = 25.8 bits (54), Expect = 7.1
 Identities = 9/23 (39%), Positives = 17/23 (73%)
 Frame = +2

Query: 467 HGDLARIGRCSANGIVKGIGLSN 535
           H D++++ R  A+  +KGIGL++
Sbjct: 8   HNDVSKLERIGAHSHIKGIGLND 30


>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
            Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2199

 Score = 25.4 bits (53), Expect = 9.4
 Identities = 11/23 (47%), Positives = 15/23 (65%)
 Frame = -1

Query: 441  RHLIQWASASNKDLYNPDLVSSV 373
            +HL +W S+S   LY+P L S V
Sbjct: 1780 QHLARWISSSKSYLYSPLLSSHV 1802


>SPCC14G10.04 |||sequence orphan|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 497

 Score = 25.4 bits (53), Expect = 9.4
 Identities = 10/31 (32%), Positives = 17/31 (54%)
 Frame = -3

Query: 223 RYRNDGIRHLWRALQHHGAACPWTSLRRSDQ 131
           RYRN        ++ H G+  PW+S+ R ++
Sbjct: 113 RYRNGASVSRSSSIGHSGSTAPWSSVGRHNR 143


>SPAC637.12c |mst1||histone acetyltransferase
           Mst1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 463

 Score = 25.4 bits (53), Expect = 9.4
 Identities = 9/18 (50%), Positives = 12/18 (66%)
 Frame = -1

Query: 453 NDYNRHLIQWASASNKDL 400
           NDYN+ L +W +  N DL
Sbjct: 57  NDYNKRLDEWITIDNIDL 74


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,371,850
Number of Sequences: 5004
Number of extensions: 70144
Number of successful extensions: 227
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 205
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 223
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 383374054
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -