Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30982
(614 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC18G6.14c |rps7||40S ribosomal protein S7|Schizosaccharomyces... 106 3e-24
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 31 0.13
SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces... 26 3.8
SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr... 25 8.7
SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3 Rfp1|Schizo... 25 8.7
SPCP1E11.05c |||sterol O-acyltransferase |Schizosaccharomyces po... 25 8.7
SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|... 25 8.7
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 25 8.7
>SPAC18G6.14c |rps7||40S ribosomal protein S7|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 195
Score = 106 bits (254), Expect = 3e-24
Identities = 51/93 (54%), Positives = 70/93 (75%), Gaps = 1/93 (1%)
Frame = +2
Query: 251 KEFSGKHVVFVGDRKILPKPSHKTRVANKQKRPRSRTLTSVYDAILEDLVFPAEIVGKRI 430
K+F+ +HV+F+ R+ILPKP K+RV QKRPRSRTLT+V++AILED+VFP EI+GKR
Sbjct: 85 KKFADRHVIFIAQRRILPKPGRKSRVT--QKRPRSRTLTAVHNAILEDIVFPTEIIGKRT 142
Query: 431 RVKLDGSQLIKVHLDKNQQTTIEHKV-TPSSLY 526
R DG + IKV LD T+++K+ + SS+Y
Sbjct: 143 RQATDGRKTIKVFLDNRDANTVDYKLGSFSSVY 175
Score = 72.9 bits (171), Expect = 3e-14
Identities = 37/80 (46%), Positives = 55/80 (68%), Gaps = 2/80 (2%)
Frame = +3
Query: 21 KIIKASGAEADSFETSISQALVELETNS-DLKAQLRELYITKAKEIELHN-KKSIIIYVP 194
KI+K S ++ + ++Q L +LE++S D+ +LR L IT A+E+E+ KK+I+++VP
Sbjct: 6 KIVKRSSSQPTETDLLVAQCLYDLESSSKDMAKELRPLQITSAREVEVGGGKKAIVVFVP 65
Query: 195 MPKLKAFQKIQIRLVRELEK 254
P LKAF K Q RL RELEK
Sbjct: 66 QPLLKAFHKCQARLTRELEK 85
Score = 34.7 bits (76), Expect = 0.011
Identities = 14/19 (73%), Positives = 16/19 (84%)
Frame = +1
Query: 511 TFQSVYKKLTGREVTFEFP 567
+F SVY KLTG+ VTFEFP
Sbjct: 170 SFSSVYHKLTGKNVTFEFP 188
>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1828
Score = 31.1 bits (67), Expect = 0.13
Identities = 25/86 (29%), Positives = 38/86 (44%), Gaps = 1/86 (1%)
Frame = +2
Query: 254 EFSGKHVVFVGDRKILPKPSHKTRVANKQKRPRSRTLTSVYDAILEDLVFPAEIVGKRIR 433
E + H V V D+ + P N+Q R +L+ + D + + V E V R
Sbjct: 1589 EENRSHTVLVLDKSVHQFPWESLPCLNRQSVSRVPSLSILRDILSQSFVVNGEYVEVR-- 1646
Query: 434 VKLDGSQLIKVHLD-KNQQTTIEHKV 508
K GS ++ LD K+ Q EHK+
Sbjct: 1647 -KEAGSYILNPSLDLKHTQEMFEHKL 1671
>SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 404
Score = 26.2 bits (55), Expect = 3.8
Identities = 9/23 (39%), Positives = 16/23 (69%)
Frame = +1
Query: 340 KEATLKDIDLCVRCYPRGLGLPC 408
++AT++++D C C RGL + C
Sbjct: 110 RKATIRNVDYCSACGGRGLFICC 132
>SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr
1|||Manual
Length = 703
Score = 25.0 bits (52), Expect = 8.7
Identities = 11/26 (42%), Positives = 19/26 (73%)
Frame = -2
Query: 358 P*AWPLLFVSNTSFVAGLRQDLTVSN 281
P A +LF+S TSF++G+ Q + ++N
Sbjct: 373 PPAAMILFISCTSFISGILQLVLLNN 398
>SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3
Rfp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 254
Score = 25.0 bits (52), Expect = 8.7
Identities = 13/42 (30%), Positives = 22/42 (52%)
Frame = +2
Query: 302 PKPSHKTRVANKQKRPRSRTLTSVYDAILEDLVFPAEIVGKR 427
P S + R N+++ RSR S + + LED+++ V R
Sbjct: 49 PVLSPRRRRMNRRRNERSRNFPSNHLSYLEDMIYLGPQVSTR 90
>SPCP1E11.05c |||sterol O-acyltransferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 472
Score = 25.0 bits (52), Expect = 8.7
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = -3
Query: 513 GVTLCSIVVCWFLSKCTLMSC 451
GV L SI++ FLS C L C
Sbjct: 138 GVYLYSILILLFLSHCVLRCC 158
>SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 532
Score = 25.0 bits (52), Expect = 8.7
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = -3
Query: 489 VCWFLSKCTLMSCEPSNLTLMR 424
+C FL K T SC NL L++
Sbjct: 207 LCSFLPKSTYRSCRSDNLDLLK 228
>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1576
Score = 25.0 bits (52), Expect = 8.7
Identities = 18/61 (29%), Positives = 30/61 (49%), Gaps = 5/61 (8%)
Frame = +3
Query: 120 LRELYITKAKEIELHNKKSIIIYVPMPKLK-----AFQKIQIRLVRELEKSSVVNM*SLL 284
L +L + + I HN K + P P LK A +++ + LV + K S++ SLL
Sbjct: 1292 LEKLVFSMLRYIRFHNSKQSSKHSPSPTLKSVGPWAPRRVDV-LVTSIGKDSILEKCSLL 1350
Query: 285 E 287
+
Sbjct: 1351 Q 1351
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,394,384
Number of Sequences: 5004
Number of extensions: 48243
Number of successful extensions: 138
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 137
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 269634532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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