Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30970
(685 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 171 8e-44
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 167 1e-42
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 74 2e-14
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 57 3e-09
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 34 0.022
SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 28 1.1
SPBC428.07 |meu6||meiotic chromosome segregation protein Meu6|Sc... 27 1.9
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 26 5.8
SPAC824.02 |||GPI inositol deacylase|Schizosaccharomyces pombe|c... 26 5.8
SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 25 7.7
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 25 7.7
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 171 bits (416), Expect = 8e-44
Identities = 87/144 (60%), Positives = 98/144 (68%), Gaps = 1/144 (0%)
Frame = +1
Query: 256 NEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRI 435
NE+ YDICRRNLDIERP+Y NLNRLI Q+VSSITASLRF+G+LNVDL EFQTNLVPYPRI
Sbjct: 206 NESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRI 265
Query: 436 HFPLVTYAPVISAEKAYHEQLSVAGSQTHASSPPTRW*NATPVMASTWLAVCCTVVTS-Y 612
HFPLVTYAP++SA KA+HE SV P + P A ++A C
Sbjct: 266 HFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDP-RAGRYMATCLLYRGDVI 324
Query: 613 PRM*TRPSLTIKTKRTIQFRRWCP 684
PR TIK KRTIQF WCP
Sbjct: 325 PRDVQAAVTTIKAKRTIQFVDWCP 348
Score = 118 bits (284), Expect = 8e-28
Identities = 51/84 (60%), Positives = 67/84 (79%)
Frame = +2
Query: 2 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 181
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 182 VVEPYNSILTTHTTLEHSDCAFMV 253
VVEPYNS+LTTH TL+ +DC FMV
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMV 204
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 167 bits (406), Expect = 1e-42
Identities = 85/144 (59%), Positives = 97/144 (67%), Gaps = 1/144 (0%)
Frame = +1
Query: 256 NEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRI 435
NEA YDICRRNLDIERPTY NLNRLI Q+VSSITASLRF G+LNVDL EFQTNLVPYPRI
Sbjct: 210 NEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRI 269
Query: 436 HFPLVTYAPVISAEKAYHEQLSVAGSQTHASSPPTRW*NATPVMASTWLAVCCTVVTS-Y 612
HFPLVTY+P++SA KA+HE SV P + P ++A C
Sbjct: 270 HFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDP-RTGRYMATCLLYRGDVI 328
Query: 613 PRM*TRPSLTIKTKRTIQFRRWCP 684
PR +IK++RTIQF WCP
Sbjct: 329 PRDVQAAVTSIKSRRTIQFVDWCP 352
Score = 123 bits (297), Expect = 2e-29
Identities = 53/84 (63%), Positives = 68/84 (80%)
Frame = +2
Query: 2 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 181
RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAPQVST+
Sbjct: 125 RIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTS 184
Query: 182 VVEPYNSILTTHTTLEHSDCAFMV 253
VVEPYNS+LTTH TL++SDC FMV
Sbjct: 185 VVEPYNSVLTTHATLDNSDCTFMV 208
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 73.7 bits (173), Expect = 2e-14
Identities = 32/83 (38%), Positives = 52/83 (62%)
Frame = +1
Query: 256 NEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRI 435
NEA+ I L I+ P+Y +LN L+ +++ +T S RF G LN DL + N+VP+PR+
Sbjct: 204 NEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRL 263
Query: 436 HFPLVTYAPVISAEKAYHEQLSV 504
HF +V +AP+ + + + +SV
Sbjct: 264 HFFMVGFAPLAAIGSSSFQAVSV 286
Score = 61.3 bits (142), Expect = 1e-10
Identities = 28/83 (33%), Positives = 45/83 (54%)
Frame = +2
Query: 5 IRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAV 184
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S V
Sbjct: 120 VRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTV 179
Query: 185 VEPYNSILTTHTTLEHSDCAFMV 253
VEPYN+ L+ H +E+SD F +
Sbjct: 180 VEPYNATLSMHQLVENSDETFCI 202
Score = 46.4 bits (105), Expect = 4e-06
Identities = 22/53 (41%), Positives = 31/53 (58%), Gaps = 2/53 (3%)
Frame = +3
Query: 510 ITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIANHQ--NQAYY 662
+T F+ N MV DPRHG+Y+ L+RG V K+V+ I + Q N AY+
Sbjct: 289 LTQQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYF 341
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 56.8 bits (131), Expect = 3e-09
Identities = 25/69 (36%), Positives = 39/69 (56%)
Frame = +1
Query: 256 NEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRI 435
N A+ I L + PT+ N+L+ ++S+ T +LR+ G +N DL +L+P PR
Sbjct: 207 NAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRC 266
Query: 436 HFPLVTYAP 462
HF L +Y P
Sbjct: 267 HFLLTSYTP 275
Score = 44.0 bits (99), Expect = 2e-05
Identities = 25/69 (36%), Positives = 36/69 (52%), Gaps = 1/69 (1%)
Frame = +2
Query: 5 IRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VSTA 181
I + AD L+GF + H L+ERL+ Y KK ++++P Q VS
Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDV 181
Query: 182 VVEPYNSIL 208
VV+PYNS+L
Sbjct: 182 VVQPYNSLL 190
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 33.9 bits (74), Expect = 0.022
Identities = 18/54 (33%), Positives = 30/54 (55%)
Frame = -2
Query: 423 RHQVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 262
R + E + H ++ + Q+SSD ++ +D T VG+G AL I + D +DG
Sbjct: 165 RRVLQAERAKEHRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPCC18.03 |||shuttle craft like transcriptional
regulator|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1077
Score = 28.3 bits (60), Expect = 1.1
Identities = 14/42 (33%), Positives = 18/42 (42%)
Frame = +3
Query: 510 ITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAI 635
+ ACFEP N C H K C L + KD N ++
Sbjct: 361 LCGACFEPINAKCYCG-LHSKTYPCSSLPSPSISKKDENGSV 401
>SPBC428.07 |meu6||meiotic chromosome segregation protein
Meu6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 651
Score = 27.5 bits (58), Expect = 1.9
Identities = 20/64 (31%), Positives = 29/64 (45%), Gaps = 2/64 (3%)
Frame = +1
Query: 379 ALNVDLTEFQTNLVPYPRIHFPLVT--YAPVISAEKAYHEQLSVAGSQTHASSPPTRW*N 552
A +V++ E +TN +P HFP T AP S EK + S + T+ N
Sbjct: 368 AQSVEVPENETNQIPTTEEHFPATTEEVAPA-SEEKPATGPAEESTSTQNVEQASTQNDN 426
Query: 553 ATPV 564
TP+
Sbjct: 427 GTPI 430
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 25.8 bits (54), Expect = 5.8
Identities = 10/20 (50%), Positives = 11/20 (55%)
Frame = -2
Query: 615 WVRRHHGTAYSKPCTCHDGG 556
W R H Y+KPC DGG
Sbjct: 16 WRRDHPFGFYAKPCKSSDGG 35
>SPAC824.02 |||GPI inositol deacylase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1142
Score = 25.8 bits (54), Expect = 5.8
Identities = 13/57 (22%), Positives = 26/57 (45%)
Frame = -3
Query: 575 VLAMTGVAFHHLVGGLEACVCDPATESCSW*AFSAEMTGAYVTSGKWIRG*GTKLVW 405
++++ +AF +L+ +E V D A SW F+ + ++ + G T W
Sbjct: 788 LVSILSIAFSYLISRVELIVADGADPVASWKIFAMMVPKSFWKQNHLLFGLQTAQFW 844
>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 330
Score = 25.4 bits (53), Expect = 7.7
Identities = 16/50 (32%), Positives = 20/50 (40%)
Frame = +1
Query: 229 AL*LCFHGRNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDG 378
AL CF A YDI +R E L R++ I+ A L G
Sbjct: 225 ALNECFKETKNAAYDIIQRKGSTEYGVAAGLVRILAAIIRDENALLTVSG 274
>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 3227
Score = 25.4 bits (53), Expect = 7.7
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = -3
Query: 638 SDGRVHILGYDVTTVQHTASHVLAMTGVAFHHLVGG 531
S G +LGY ++ A++V+A + V HL+ G
Sbjct: 407 SAGLTSLLGYHLSVKTPQATYVVARSIVMLDHLIDG 442
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,887,985
Number of Sequences: 5004
Number of extensions: 59791
Number of successful extensions: 193
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 188
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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