Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30827
(727 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 96 4e-21
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 73 6e-14
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 71 1e-13
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 49 6e-07
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 44 2e-05
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 40 4e-04
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 38 0.001
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 38 0.002
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 29 0.51
SPAC22F3.11c |snu23||U4/U6 x U5 tri-snRNP complex subunit Snu23|... 27 2.7
SPBC3H7.10 |||elongator homolog|Schizosaccharomyces pombe|chr 2|... 25 8.3
SPBC839.07 |ibp1||itty bitty phosphatase Ibp1|Schizosaccharomyce... 25 8.3
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 96.3 bits (229), Expect = 4e-21
Identities = 53/145 (36%), Positives = 71/145 (48%), Gaps = 4/145 (2%)
Frame = +1
Query: 274 IIEFFAPWCGHCKSLVPEYKKAARALK--GIVKVGALDADEHRSVSQKYGVTGFPTIKIF 447
+IEF+A WCGHCKSL P Y++ + V +G +DAD H V+ KY +TGFPT+ F
Sbjct: 43 LIEFYATWCGHCKSLAPVYEELGALFEDHNDVLIGKIDADTHSDVADKYHITGFPTLIWF 102
Query: 448 --TGSKHTPYQGQRTAEGFVEAV*TQPRRRHMXNLXXXXXXXXXXXXXVITLTDSNFKEL 621
GS+ Y R + + V V+ L NF ++
Sbjct: 103 PPDGSEPVQYSNARDVDSLTQFV---------SEKTGIKKRKIVLPSNVVELDSLNFDKV 153
Query: 622 VLDSDDLWLVEFYAPWCGHCKNLEP 696
V+D LVEFYA WCG+CK L P
Sbjct: 154 VMDDKKDVLVEFYADWCGYCKRLAP 178
Score = 60.5 bits (140), Expect = 2e-10
Identities = 29/84 (34%), Positives = 46/84 (54%), Gaps = 5/84 (5%)
Frame = +1
Query: 274 IIEFFAPWCGHCKSLVPEYKKAARALKG--IVKVGALDADEHRSVSQKYGVTGFPTIKIF 447
++EF+A WCG+CK L P Y+ + K V++ ++AD + + + V FPTIK F
Sbjct: 162 LVEFYADWCGYCKRLAPTYETLGKVFKNEPNVEIVKINADVFADIGRLHEVASFPTIKFF 221
Query: 448 -TGSKHTP--YQGQRTAEGFVEAV 510
K P Y+G R+ E +E +
Sbjct: 222 PKDDKDKPELYEGDRSLESLIEYI 245
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 72.5 bits (170), Expect = 6e-14
Identities = 30/84 (35%), Positives = 52/84 (61%), Gaps = 2/84 (2%)
Frame = +1
Query: 259 SDEIWIIEFFAPWCGHCKSLVPEYKKAARAL-KGIVKVGALDADEHRSVSQKYGVTGFPT 435
+D++ +++F+APWCGHCK+L PEY+ AA L K + + +D E + +Y + G+PT
Sbjct: 38 ADKVLMVKFYAPWCGHCKALAPEYESAADELEKDGISLVEVDCTEEGDLCSEYSIRGYPT 97
Query: 436 IKIF-TGSKHTPYQGQRTAEGFVE 504
+ +F G + + Y G R + V+
Sbjct: 98 LNVFKNGKQISQYSGPRKHDALVK 121
Score = 61.3 bits (142), Expect = 1e-10
Identities = 34/78 (43%), Positives = 45/78 (57%), Gaps = 5/78 (6%)
Frame = +1
Query: 274 IIEFFAPWCGHCKSLVPEYKKAARALK--GIVKVGALDADEHRSVSQKYGVTGFPTIKIF 447
++EF+APWCGHCK+L P Y+K A V V +DA E+ +S ++GFPTI F
Sbjct: 377 LVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVAKIDATEN-DIS--VSISGFPTIMFF 433
Query: 448 -TGSKHTP--YQGQRTAE 492
K P Y+G RT E
Sbjct: 434 KANDKVNPVRYEGDRTLE 451
Score = 58.8 bits (136), Expect = 7e-10
Identities = 24/45 (53%), Positives = 30/45 (66%)
Frame = +1
Query: 586 VITLTDSNFKELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATE 720
++ L NF ++V+D LVEFYAPWCGHCKNL P + K A E
Sbjct: 357 LVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEE 401
Score = 49.2 bits (112), Expect = 6e-07
Identities = 18/35 (51%), Positives = 26/35 (74%)
Frame = +1
Query: 622 VLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELK 726
++ +D + +V+FYAPWCGHCK L P + AA EL+
Sbjct: 35 LITADKVLMVKFYAPWCGHCKALAPEYESAADELE 69
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 71.3 bits (167), Expect = 1e-13
Identities = 36/79 (45%), Positives = 51/79 (64%), Gaps = 8/79 (10%)
Frame = +1
Query: 274 IIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGAL--DADEHRSVSQKYGVTGFPTIK-I 444
++ F+APWCG+CK LVP Y+K A L ++ V A+ DAD++R+V +Y V GFPTIK +
Sbjct: 52 LVVFYAPWCGYCKKLVPTYQKLASNLHSLLPVTAVDCDADQNRAVCSQYQVQGFPTIKLV 111
Query: 445 FTGSK-----HTPYQGQRT 486
+ SK T Y G R+
Sbjct: 112 YPSSKGSSLSSTDYNGDRS 130
Score = 44.4 bits (100), Expect = 2e-05
Identities = 21/45 (46%), Positives = 26/45 (57%)
Frame = +1
Query: 589 ITLTDSNFKELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATEL 723
I L NF++ V LV FYAPWCG+CK L P + K A+ L
Sbjct: 34 IELNSKNFRKFVKAKGPS-LVVFYAPWCGYCKKLVPTYQKLASNL 77
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 49.2 bits (112), Expect = 6e-07
Identities = 21/64 (32%), Positives = 40/64 (62%)
Frame = +1
Query: 256 NSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGVTGFPT 435
++D++ +++F+A WCG CK L P +K + + + A++AD+ ++QK GV PT
Sbjct: 33 SADKVTVVDFYADWCGPCKYLKPFLEKLSEQNQKASFI-AVNADKFSDIAQKNGVYALPT 91
Query: 436 IKIF 447
+ +F
Sbjct: 92 MVLF 95
Score = 36.3 bits (80), Expect = 0.004
Identities = 13/32 (40%), Positives = 21/32 (65%)
Frame = +1
Query: 625 LDSDDLWLVEFYAPWCGHCKNLEPHWAKAATE 720
+ +D + +V+FYA WCG CK L+P K + +
Sbjct: 32 ISADKVTVVDFYADWCGPCKYLKPFLEKLSEQ 63
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 44.0 bits (99), Expect = 2e-05
Identities = 19/78 (24%), Positives = 41/78 (52%), Gaps = 1/78 (1%)
Frame = +1
Query: 262 DEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGVTGFPTIK 441
D++ +++FFA WCG CK++ P++++ + + +D D+ ++ + GV P+
Sbjct: 18 DKLVVVDFFATWCGPCKAIAPKFEQFSNTYSDATFI-KVDVDQLSEIAAEAGVHAMPSFF 76
Query: 442 IF-TGSKHTPYQGQRTAE 492
++ G K G A+
Sbjct: 77 LYKNGEKIEEIVGANPAK 94
Score = 35.5 bits (78), Expect = 0.008
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = +1
Query: 604 SNFKELVLDSDDLWLVEFYAPWCGHCKNLEP 696
S FK +V D L +V+F+A WCG CK + P
Sbjct: 9 SEFKSIVCQ-DKLVVVDFFATWCGPCKAIAP 38
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 39.9 bits (89), Expect = 4e-04
Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 1/62 (1%)
Frame = +1
Query: 277 IEFFAPWCGHCKSLVPEYKKAARALKGIVKVGA-LDADEHRSVSQKYGVTGFPTIKIFTG 453
++ +A WCG CK++ P + + A V A ++ DE R ++ GV PT F
Sbjct: 24 VDCYADWCGPCKAISPLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFFEN 83
Query: 454 SK 459
K
Sbjct: 84 GK 85
Score = 29.9 bits (64), Expect = 0.39
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +1
Query: 649 VEFYAPWCGHCKNLEPHWAKAATE 720
V+ YA WCG CK + P +++ A++
Sbjct: 24 VDCYADWCGPCKAISPLFSQLASK 47
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 38.3 bits (85), Expect = 0.001
Identities = 20/77 (25%), Positives = 40/77 (51%), Gaps = 1/77 (1%)
Frame = +1
Query: 232 E*FRQIS*NSDE-IWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQ 408
E F++I N E I ++ F+APW CK + + + A+ K V + ++A++ +++
Sbjct: 9 EQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAVFL-KIEAEKFSDIAE 67
Query: 409 KYGVTGFPTIKIFTGSK 459
+ V P + G+K
Sbjct: 68 SFDVNAVPLFVLIHGAK 84
Score = 28.7 bits (61), Expect = 0.89
Identities = 12/40 (30%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +1
Query: 610 FKELVLDS-DDLWLVEFYAPWCGHCKNLEPHWAKAATELK 726
F+E++ + + + L+ FYAPW CK + + + A + K
Sbjct: 11 FQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTK 50
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 37.5 bits (83), Expect = 0.002
Identities = 16/46 (34%), Positives = 24/46 (52%)
Frame = +1
Query: 589 ITLTDSNFKELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELK 726
+ LTD++ + V S W +++Y P CG CK L P W + K
Sbjct: 29 VPLTDNDLESEV--SKGTWFIKYYLPSCGACKRLGPMWDNMVEKAK 72
Score = 36.3 bits (80), Expect = 0.004
Identities = 16/71 (22%), Positives = 30/71 (42%)
Frame = +1
Query: 265 EIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGVTGFPTIKI 444
E W I+F++ C C + + A ++G + V ++ + ++Y + FPT
Sbjct: 299 EGWFIQFYSSECDDCDDVSTAWYAMANRMRGKLNVAHINCAVSKRACKQYSIQYFPTFLF 358
Query: 445 FTGSKHTPYQG 477
F Y G
Sbjct: 359 FKEEAFVEYVG 369
Score = 29.5 bits (63), Expect = 0.51
Identities = 15/62 (24%), Positives = 27/62 (43%), Gaps = 3/62 (4%)
Frame = +1
Query: 271 WIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGAL---DADEHRSVSQKYGVTGFPTIK 441
W I+++ P CG CK L P + K V+ + D + +S + PT+
Sbjct: 45 WFIKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVDCSKELSSCANIRAVPTLY 104
Query: 442 IF 447
++
Sbjct: 105 LY 106
Score = 26.6 bits (56), Expect = 3.6
Identities = 8/37 (21%), Positives = 17/37 (45%)
Frame = +1
Query: 616 ELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELK 726
+ L + W ++FY+ C C ++ W A ++
Sbjct: 292 DAALTDKEGWFIQFYSSECDDCDDVSTAWYAMANRMR 328
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 29.5 bits (63), Expect = 0.51
Identities = 13/60 (21%), Positives = 24/60 (40%)
Frame = +1
Query: 298 CGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGVTGFPTIKIFTGSKHTPYQG 477
C C + R +K+ ++ DE + + + + FPT ++F G Y G
Sbjct: 209 CEDCFHWEAVWSSITRNTDERLKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYNG 268
>SPAC22F3.11c |snu23||U4/U6 x U5 tri-snRNP complex subunit
Snu23|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 27.1 bits (57), Expect = 2.7
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = -2
Query: 288 KELNDPNFVGILTNLSKLLGVSSITS 211
KE N P VG T L+ ++G+SS S
Sbjct: 123 KEKNSPRLVGENTELAAIMGISSFGS 148
>SPBC3H7.10 |||elongator homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 249
Score = 25.4 bits (53), Expect = 8.3
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = +3
Query: 330 QKSCESSKGHCKSRCLRC 383
QKS SS GH +RC+ C
Sbjct: 172 QKSLGSSIGHMATRCISC 189
>SPBC839.07 |ibp1||itty bitty phosphatase Ibp1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 138
Score = 25.4 bits (53), Expect = 8.3
Identities = 17/51 (33%), Positives = 24/51 (47%), Gaps = 1/51 (1%)
Frame = +1
Query: 304 HCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGV-TGFPTIKIFTG 453
HC KAAR L I++ ++ E S+SQK + PT+ I G
Sbjct: 69 HCTYSQVRGPKAARVLSEILRNRITESKEKLSLSQKEKLFQNLPTVYILHG 119
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,957,390
Number of Sequences: 5004
Number of extensions: 59811
Number of successful extensions: 199
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 196
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 341222980
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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