Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30821
(778 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 71 1e-13
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 50 4e-07
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 42 1e-04
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 37 0.004
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 36 0.005
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 33 0.035
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 30 0.43
SPCC553.04 |cyp9||WD repeat containing cyclophilin family peptid... 28 1.3
SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomy... 28 1.7
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 27 2.3
SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos... 27 4.0
SPAC6F6.11c |||pyridoxine-pyridoxal-pyridoxamine kinase |Schizos... 26 6.9
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 25 9.2
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 25 9.2
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 71.3 bits (167), Expect = 1e-13
Identities = 29/63 (46%), Positives = 46/63 (73%)
Frame = +2
Query: 272 VALAKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSSEYNGPRESNGIVKYMRAQVGPSS 451
++L +VDCTE G C ++S+ GYPTL +F+ G+ S+Y+GPR+ + +VKYMR Q+ P+
Sbjct: 73 ISLVEVDCTEEG-DLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQLLPTV 131
Query: 452 KEL 460
K +
Sbjct: 132 KPI 134
Score = 48.4 bits (110), Expect = 1e-06
Identities = 21/51 (41%), Positives = 29/51 (56%)
Frame = +3
Query: 93 YLCKAAEEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAA 245
+ C +AE V + + +++ +V FYAPWCGHCK L PEY AA
Sbjct: 17 FFCASAE--VPKVNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAA 65
Score = 47.6 bits (108), Expect = 2e-06
Identities = 20/43 (46%), Positives = 27/43 (62%), Gaps = 1/43 (2%)
Frame = +3
Query: 108 AEEDVLDLTDSDFS-AVLSQHDTALVMFYAPWCGHCKRLKPEY 233
++ED++ L +F V+ + LV FYAPWCGHCK L P Y
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTY 395
Score = 29.5 bits (63), Expect = 0.56
Identities = 26/91 (28%), Positives = 42/91 (46%), Gaps = 4/91 (4%)
Frame = +2
Query: 257 TDVPPVALAKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSS--EYNGPRESNGIVKYM- 427
+D V +AK+D TE S S+SG+PT+ F+ + + Y G R + ++
Sbjct: 403 SDDSNVVVAKIDATENDISV----SISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFID 458
Query: 428 -RAQVGPSSKELLTVADFEAFTSKDEVVLSD 517
A P KE +V A +D+V + D
Sbjct: 459 KHASFEPIKKEKESV---PAPDLEDQVAVED 486
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 50.0 bits (114), Expect = 4e-07
Identities = 24/78 (30%), Positives = 39/78 (50%)
Frame = +3
Query: 30 KAPAKFKMFGSLKFVLLLGIIYLCKAAEEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGH 209
+ P F +F + F L+ G+ + ++L +F + +LV+FYAPWCG+
Sbjct: 4 RIPTLFTLFLAC-FSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGY 62
Query: 210 CKRLKPEYAVAAGLLRPM 263
CK+L P Y A L +
Sbjct: 63 CKKLVPTYQKLASNLHSL 80
Score = 39.9 bits (89), Expect = 4e-04
Identities = 29/86 (33%), Positives = 45/86 (52%), Gaps = 6/86 (6%)
Frame = +2
Query: 287 VDC-TEGGKSTCEQFSVSGYPTLKIF---RKGE--LSSEYNGPRESNGIVKYMRAQVGPS 448
VDC + ++ C Q+ V G+PT+K+ KG S++YNG R + K++ + PS
Sbjct: 86 VDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRSYKSLQKFVSDSI-PS 144
Query: 449 SKELLTVADFEAFTSKDEVVLSDSSK 526
++LT EA T K +SSK
Sbjct: 145 KVKILT---SEAKTQKFIQDAQNSSK 167
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 41.5 bits (93), Expect = 1e-04
Identities = 18/40 (45%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = +3
Query: 117 DVLDLTDSDFS-AVLSQHDTALVMFYAPWCGHCKRLKPEY 233
+V++L +F V+ LV FYA WCG+CKRL P Y
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTY 180
Score = 39.1 bits (87), Expect = 7e-04
Identities = 14/20 (70%), Positives = 15/20 (75%)
Frame = +3
Query: 174 ALVMFYAPWCGHCKRLKPEY 233
AL+ FYA WCGHCK L P Y
Sbjct: 42 ALIEFYATWCGHCKSLAPVY 61
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 36.7 bits (81), Expect = 0.004
Identities = 12/32 (37%), Positives = 21/32 (65%)
Frame = +3
Query: 138 SDFSAVLSQHDTALVMFYAPWCGHCKRLKPEY 233
S+F +++ Q +V F+A WCG CK + P++
Sbjct: 9 SEFKSIVCQDKLVVVDFFATWCGPCKAIAPKF 40
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 36.3 bits (80), Expect = 0.005
Identities = 14/29 (48%), Positives = 18/29 (62%)
Frame = +3
Query: 141 DFSAVLSQHDTALVMFYAPWCGHCKRLKP 227
D++ +S +V FYA WCG CK LKP
Sbjct: 27 DYNTRISADKVTVVDFYADWCGPCKYLKP 55
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 33.5 bits (73), Expect = 0.035
Identities = 15/33 (45%), Positives = 21/33 (63%)
Frame = +3
Query: 129 LTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKP 227
LTD+D + +S+ T + +Y P CG CKRL P
Sbjct: 31 LTDNDLESEVSK-GTWFIKYYLPSCGACKRLGP 62
Score = 28.3 bits (60), Expect = 1.3
Identities = 15/49 (30%), Positives = 25/49 (51%)
Frame = +2
Query: 278 LAKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSSEYNGPRESNGIVKY 424
+A ++C K C+Q+S+ +PT +F K E EY G +V +
Sbjct: 333 VAHINCAVS-KRACKQYSIQYFPTF-LFFKEEAFVEYVGLPNEGDLVSF 379
>SPCC162.08c |nup211||nuclear pore complex associated
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1837
Score = 29.9 bits (64), Expect = 0.43
Identities = 25/94 (26%), Positives = 43/94 (45%), Gaps = 10/94 (10%)
Frame = +1
Query: 526 KESDLKGEFLKTADKLREEVTFAHSSANEVLEK---------TGYKNNVVLYRPKRLQNK 678
+E D F K +K+R + +S E +EK + Y++ +V + R K
Sbjct: 1042 REKDSALSFKKDYEKIRSDADRVITSLKEDIEKERSLMKECHSNYESEIVSHG--RTTQK 1099
Query: 679 FEDSSVAFDG-DTEKVSLKAFIKENYHGLSGVRQ 777
D FD +T+ + LKA ++ + GLSG +
Sbjct: 1100 LRDLRTEFDEVNTKYLKLKANFEQQHSGLSGAEK 1133
>SPCC553.04 |cyp9||WD repeat containing cyclophilin family
peptidyl-prolyl cis-trans isomerase
Cyp9|Schizosaccharomyces pombe|chr 3|||Manual
Length = 610
Score = 28.3 bits (60), Expect = 1.3
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = -3
Query: 623 FSRTSLADE*AKVTSSLNLSAVFRNSPFRSDSFSKNPT 510
F+R SL + K ++ +L + N+P +SF K+PT
Sbjct: 366 FTRLSLYQQAPKKSNLPSLDVIASNNPLVEESFQKDPT 403
>SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2052
Score = 27.9 bits (59), Expect = 1.7
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = +1
Query: 586 TFAHSSANEVLEKTGYKNNVVLYRPKRLQNKFEDSSVAF 702
T + S +NE+ EKT K + L + K++ +F D +F
Sbjct: 1265 TSSTSDSNEI-EKTQEKKRLALEKQKKIMQQFRDQQASF 1302
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 27.5 bits (58), Expect = 2.3
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = +3
Query: 189 YAPWCGHCKRLKPEYAVAA 245
YA WCG CK + P ++ A
Sbjct: 27 YADWCGPCKAISPLFSQLA 45
>SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 640
Score = 26.6 bits (56), Expect = 4.0
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = +1
Query: 121 FSILQIPTFRLFYLNMIQPWSCF 189
F LQ+ FR + N+++PW CF
Sbjct: 208 FYCLQLQMFRKMH-NIVRPWDCF 229
>SPAC6F6.11c |||pyridoxine-pyridoxal-pyridoxamine kinase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 309
Score = 25.8 bits (54), Expect = 6.9
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = +1
Query: 565 DKLREEVTFAHSSANEVLEKT 627
DKL++ V A SS +EV++KT
Sbjct: 248 DKLKKSVEMALSSVHEVIQKT 268
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 25.4 bits (53), Expect = 9.2
Identities = 8/15 (53%), Positives = 11/15 (73%)
Frame = +3
Query: 177 LVMFYAPWCGHCKRL 221
L+ FYAPW CK++
Sbjct: 24 LLNFYAPWAAPCKQM 38
>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 3655
Score = 25.4 bits (53), Expect = 9.2
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = -1
Query: 616 ELHWLMNEQK*PPPLICQLFSGTLLSGQIPFRRI 515
E+ W +Q+ P P QLF ++ ++ FRRI
Sbjct: 3581 EVFWWSKQQRKPIPQGIQLFETVKVNVELLFRRI 3614
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,189,280
Number of Sequences: 5004
Number of extensions: 66219
Number of successful extensions: 227
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 215
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 227
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 375345278
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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