Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30722
(512 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 162 2e-41
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 162 3e-41
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 162 3e-41
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 97 2e-21
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 94 1e-20
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 74 1e-14
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 35 0.008
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 34 0.011
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 33 0.019
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 32 0.044
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 31 0.13
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 30 0.18
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 29 0.31
SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces p... 29 0.41
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 28 0.94
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 28 0.94
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 27 2.2
SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1||... 26 2.9
SPAC12D12.09 |rev7||DNA polymerase zeta Rev7 |Schizosaccharomyce... 25 6.7
SPBC11B10.05c |rsp1||random septum position protein Rsp1|Schizos... 25 6.7
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 25 6.7
SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces pomb... 25 8.8
SPAC1687.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 8.8
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 162 bits (394), Expect = 2e-41
Identities = 76/85 (89%), Positives = 80/85 (94%)
Frame = +1
Query: 1 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 180
ITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQADCAVLI+ GTGEFEAGISK+
Sbjct: 71 ITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAVLIIGGGTGEFEAGISKD 130
Query: 181 GQTREHALLAFTLGVKQLIVGVNKM 255
GQTREHALLA+TLGVKQLIV VNKM
Sbjct: 131 GQTREHALLAYTLGVKQLIVAVNKM 155
Score = 103 bits (248), Expect = 1e-23
Identities = 42/71 (59%), Positives = 56/71 (78%)
Frame = +3
Query: 273 YSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEG 452
+S+ RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G
Sbjct: 160 WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAG 219
Query: 453 KADGKCLIEAL 485
GK L+EA+
Sbjct: 220 VVKGKTLLEAI 230
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 162 bits (393), Expect = 3e-41
Identities = 75/85 (88%), Positives = 80/85 (94%)
Frame = +1
Query: 1 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 180
ITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQADCA+LI+ GTGEFEAGISK+
Sbjct: 71 ITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISKD 130
Query: 181 GQTREHALLAFTLGVKQLIVGVNKM 255
GQTREHALLA+TLGVKQLIV VNKM
Sbjct: 131 GQTREHALLAYTLGVKQLIVAVNKM 155
Score = 103 bits (248), Expect = 1e-23
Identities = 42/71 (59%), Positives = 56/71 (78%)
Frame = +3
Query: 273 YSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEG 452
+S+ RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G
Sbjct: 160 WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAG 219
Query: 453 KADGKCLIEAL 485
GK L+EA+
Sbjct: 220 VVKGKTLLEAI 230
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 162 bits (393), Expect = 3e-41
Identities = 75/85 (88%), Positives = 80/85 (94%)
Frame = +1
Query: 1 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 180
ITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQADCA+LI+ GTGEFEAGISK+
Sbjct: 71 ITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISKD 130
Query: 181 GQTREHALLAFTLGVKQLIVGVNKM 255
GQTREHALLA+TLGVKQLIV VNKM
Sbjct: 131 GQTREHALLAYTLGVKQLIVAVNKM 155
Score = 103 bits (248), Expect = 1e-23
Identities = 42/71 (59%), Positives = 56/71 (78%)
Frame = +3
Query: 273 YSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEG 452
+S+ RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G
Sbjct: 160 WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAG 219
Query: 453 KADGKCLIEAL 485
GK L+EA+
Sbjct: 220 VVKGKTLLEAI 230
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 96.7 bits (230), Expect = 2e-21
Identities = 42/84 (50%), Positives = 59/84 (70%)
Frame = +1
Query: 4 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 183
T+++ FET +++DAPGH+ ++ NMI G SQAD VL+++A GEFEAG + G
Sbjct: 303 TVEVGRAYFETEHRRFSLLDAPGHKGYVTNMINGASQADIGVLVISARRGEFEAGFERGG 362
Query: 184 QTREHALLAFTLGVKQLIVGVNKM 255
QTREHA+LA T G+ L+V +NKM
Sbjct: 363 QTREHAVLARTQGINHLVVVINKM 386
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 94.3 bits (224), Expect = 1e-20
Identities = 44/85 (51%), Positives = 56/85 (65%)
Frame = +1
Query: 1 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 180
+T+D+A FE+ K I DAPGHRDFI MI G S AD AVL+V + FE G +N
Sbjct: 241 VTMDVASTTFESDKKIYEIGDAPGHRDFISGMIAGASSADFAVLVVDSSQNNFERGFLEN 300
Query: 181 GQTREHALLAFTLGVKQLIVGVNKM 255
GQTREHA L LG+ +++V VNK+
Sbjct: 301 GQTREHAYLLRALGISEIVVSVNKL 325
Score = 45.2 bits (102), Expect = 6e-06
Identities = 24/55 (43%), Positives = 34/55 (61%), Gaps = 3/55 (5%)
Frame = +3
Query: 273 YSEPRFEEIKKEVSSY-IKKIGYNPAAVAFVPISGWHGDNML--EPSTKMPWFKG 428
+SE RF+EIK VS + IK +G+ + V FVPIS G N++ + S W+KG
Sbjct: 330 WSEDRFQEIKNIVSDFLIKMVGFKTSNVHFVPISAISGTNLIQKDSSDLYKWYKG 384
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 74.1 bits (174), Expect = 1e-14
Identities = 38/85 (44%), Positives = 55/85 (64%)
Frame = +1
Query: 1 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKN 180
ITI A ++ET+ + +D PGH D+IKNMITG + D A+++V+A G+
Sbjct: 102 ITISSAHVEYETANRHYAHVDCPGHADYIKNMITGAATMDGAIIVVSATDGQMP------ 155
Query: 181 GQTREHALLAFTLGVKQLIVGVNKM 255
QTREH LLA +GVKQ++V +NK+
Sbjct: 156 -QTREHLLLARQVGVKQIVVYINKV 179
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 34.7 bits (76), Expect = 0.008
Identities = 19/70 (27%), Positives = 33/70 (47%)
Frame = +1
Query: 46 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV 225
+V+ +D PGH + M+ G + D A+L++A QT EH + +
Sbjct: 109 HVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNES------CPQPQTSEHLAAIEIMQL 162
Query: 226 KQLIVGVNKM 255
K +I+ NK+
Sbjct: 163 KHIIILQNKV 172
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 34.3 bits (75), Expect = 0.011
Identities = 21/57 (36%), Positives = 29/57 (50%)
Frame = +1
Query: 28 FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREH 198
FE S Y + IID PGH DF + D AVL++ A +G I+ + Q R +
Sbjct: 141 FEKS-YNINIIDTPGHIDFTIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQMRRY 196
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 33.5 bits (73), Expect = 0.019
Identities = 15/42 (35%), Positives = 21/42 (50%)
Frame = +1
Query: 28 FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 153
F+ ++T +D PGH F G + AD VL+VA G
Sbjct: 215 FDKGSKFITFLDTPGHMAFEAMRKRGANIADIVVLVVAGDDG 256
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 32.3 bits (70), Expect = 0.044
Identities = 17/51 (33%), Positives = 23/51 (45%)
Frame = +1
Query: 1 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 153
ITI+ A F + +ID PGH DF + + D AV I+ G
Sbjct: 79 ITINSAAISFTWRNQRINLIDTPGHADFTFEVERSVAVLDGAVAIIDGSAG 129
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 30.7 bits (66), Expect = 0.13
Identities = 12/45 (26%), Positives = 23/45 (51%)
Frame = +1
Query: 19 LWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 153
++ + Y + +ID PGH DF ++ + + +L+V A G
Sbjct: 116 IYYYHGQSYLLNLIDTPGHVDFRAEVMHSLAACEGCILLVDASQG 160
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 30.3 bits (65), Expect = 0.18
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +1
Query: 43 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 153
Y + +ID+PGH DF + + + D A ++V A G
Sbjct: 94 YLINLIDSPGHVDFSSEVSSASRLCDGAFVLVDAVEG 130
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 29.5 bits (63), Expect = 0.31
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +1
Query: 55 IIDAPGHRDFIKNMITGTSQADCAVLIV 138
IID PGH F GTS + A+L++
Sbjct: 553 IIDTPGHESFTNLRSRGTSLCNIAILVI 580
>SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 926
Score = 29.1 bits (62), Expect = 0.41
Identities = 27/79 (34%), Positives = 37/79 (46%)
Frame = +1
Query: 136 VAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMIPLNHHTVSPDLRKSRRKYP 315
VA GT E GI NG E+A+ A +I V++ P++H + KSRR+YP
Sbjct: 47 VAEGTAE---GIVSNG---EYAVGAMGAAPTNIIGSVDQQPPVSHTS-----HKSRRQYP 95
Query: 316 XTSRRLATTQLLSLSCPFL 372
L T S + P L
Sbjct: 96 AEVFELTNTLAASPAPPSL 114
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 27.9 bits (59), Expect = 0.94
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +1
Query: 43 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 138
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 27.9 bits (59), Expect = 0.94
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +1
Query: 43 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 138
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 26.6 bits (56), Expect = 2.2
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +1
Query: 58 IDAPGHRDFIKNMITGTSQADCAVLIV 138
ID PGH DF+ + + +D VL+V
Sbjct: 214 IDTPGHVDFVDEVAAPMAISDGVVLVV 240
>SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 380
Score = 26.2 bits (55), Expect = 2.9
Identities = 12/33 (36%), Positives = 19/33 (57%), Gaps = 2/33 (6%)
Frame = +1
Query: 1 ITIDIALWKFETSKYYV--TIIDAPGHRDFIKN 93
+ I+I + E +++ T+ID PG DFI N
Sbjct: 71 VEIEITKAELEEKNFHLRLTVIDTPGFGDFINN 103
>SPAC12D12.09 |rev7||DNA polymerase zeta Rev7 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 213
Score = 25.0 bits (52), Expect = 6.7
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = -1
Query: 359 ESDSSWVVANLLDVXGYFLL 300
E+D+ W V +D+ G FLL
Sbjct: 2 ENDTGWSVKKCIDIFGEFLL 21
>SPBC11B10.05c |rsp1||random septum position protein
Rsp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 494
Score = 25.0 bits (52), Expect = 6.7
Identities = 15/45 (33%), Positives = 23/45 (51%), Gaps = 3/45 (6%)
Frame = +3
Query: 303 KEVS---SYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 428
KEVS SYI +P +G+ +N+++P + P FKG
Sbjct: 159 KEVSTSKSYISSGYLHPKTSPIFKKNGYATENVVDPISSSPRFKG 203
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 25.0 bits (52), Expect = 6.7
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = +1
Query: 370 LDGTETTCWSLQPKCLGSRDGRWSVRKAKLTENASLK 480
LD + W++QP C + DG A L++NA LK
Sbjct: 149 LDKLKDRLWNVQPTCALTGDGLLE-GLAWLSQNAKLK 184
>SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 347
Score = 24.6 bits (51), Expect = 8.8
Identities = 18/56 (32%), Positives = 27/56 (48%)
Frame = +3
Query: 255 DSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWF 422
D +P + F IKK+V Y+ ++ + P + G N LEP TK PW+
Sbjct: 108 DLKKPLLPQILFGNIKKDV--YLDQV-HRPRHYRGSGSAPLFG-NFLEPLTKTPWY 159
>SPAC1687.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 96
Score = 24.6 bits (51), Expect = 8.8
Identities = 12/26 (46%), Positives = 14/26 (53%)
Frame = -3
Query: 102 CDHVLDEISVSRSINDGNIVLASFEL 25
CDH+L IS SR G + FEL
Sbjct: 23 CDHLLQYISNSRDSKAGLKIFLVFEL 48
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,221,833
Number of Sequences: 5004
Number of extensions: 46101
Number of successful extensions: 184
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 182
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 206265012
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -