BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30714
(534 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC29B5.03c |rpl26||60S ribosomal protein L26|Schizosaccharomyc... 105 3e-24
SPAC328.03 |tps1||alpha,alpha-trehalose-phosphate synthase [UDP-... 25 5.4
SPAC22H10.03c |kap114||karyopherin Kap14|Schizosaccharomyces pom... 25 7.1
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 25 9.4
SPBC4C3.07 |||translation initiation factor eIF3f|Schizosaccharo... 25 9.4
>SPBC29B5.03c |rpl26||60S ribosomal protein L26|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 126
Score = 105 bits (253), Expect = 3e-24
Identities = 49/89 (55%), Positives = 69/89 (77%), Gaps = 1/89 (1%)
Frame = +2
Query: 32 MKFNKQVTSSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDEVQVVRG 211
MKF++ VTSSRRK RK HF APS +RRVLMS+PLSKELR+++ ++S+P+R+DD++ V+RG
Sbjct: 1 MKFSRDVTSSRRKQRKAHFGAPSSVRRVLMSAPLSKELREQYKIRSLPVRRDDQITVIRG 60
Query: 212 HYKGQQVGKVMQVYLKSLLYTL-RGFKEK 295
KG++ GK+ VY K L + R +EK
Sbjct: 61 SNKGRE-GKITSVYRKKFLLLIERVTREK 88
Score = 58.8 bits (136), Expect = 5e-10
Identities = 28/47 (59%), Positives = 37/47 (78%)
Frame = +1
Query: 256 KKFVVYIERIQREKANGATAYVGIHPSKCVIVKLKMNKDRKAILDRR 396
KKF++ IER+ REKANGA+A VGI SK VI KL ++KDRK ++ R+
Sbjct: 75 KKFLLLIERVTREKANGASAPVGIDASKVVITKLHLDKDRKDLIVRK 121
>SPAC328.03 |tps1||alpha,alpha-trehalose-phosphate synthase
[UDP-forming]|Schizosaccharomyces pombe|chr 1|||Manual
Length = 513
Score = 25.4 bits (53), Expect = 5.4
Identities = 15/42 (35%), Positives = 23/42 (54%), Gaps = 1/42 (2%)
Frame = +1
Query: 319 VGIHPSKCV-IVKLKMNKDRKAILDRRAKGRLAALGKDKGKY 441
+GI P K +K + KDR A ++RR +G +G D+ Y
Sbjct: 256 IGIDPEKFSDALKSDVVKDRIASIERRLQGVKVIVGVDRLDY 297
>SPAC22H10.03c |kap114||karyopherin Kap14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 986
Score = 25.0 bits (52), Expect = 7.1
Identities = 11/27 (40%), Positives = 13/27 (48%)
Frame = -1
Query: 441 VFTLVFAKCSQSALCSAIEDCFAVFIH 361
+F LV S +C IED F IH
Sbjct: 588 LFNLVATNASDPYICGIIEDTFEDIIH 614
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 24.6 bits (51), Expect = 9.4
Identities = 8/40 (20%), Positives = 20/40 (50%)
Frame = +1
Query: 7 CRFGEERQNEVQQAGDFLKKEKQEEAFQCSFTYKASVDVL 126
C F ++ +E++ G +L + +F C + V+++
Sbjct: 3300 CEFHHQKFDEIEVPGQYLLHKDNNNSFSCIERFLPEVELI 3339
>SPBC4C3.07 |||translation initiation factor
eIF3f|Schizosaccharomyces pombe|chr 2|||Manual
Length = 302
Score = 24.6 bits (51), Expect = 9.4
Identities = 14/48 (29%), Positives = 21/48 (43%)
Frame = +1
Query: 229 GWQSDAGVSKKFVVYIERIQREKANGATAYVGIHPSKCVIVKLKMNKD 372
GW + + F I+ + A TA +G +P C V L +N D
Sbjct: 104 GWYATSPDLDAFSALIQNLYASPAEPGTAPLGTYPHPC--VHLTVNTD 149
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,028,293
Number of Sequences: 5004
Number of extensions: 41500
Number of successful extensions: 128
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 121
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 128
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 220420454
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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