Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30690
(835 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 156 4e-39
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 156 4e-39
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 156 4e-39
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 77 2e-15
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 74 2e-14
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 49 7e-07
SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces pomb... 31 0.27
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 30 0.35
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 29 1.1
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 29 1.1
SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz... 28 1.9
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 4.3
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 26 5.7
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 26 7.6
SPCC1442.04c |||conserved fungal protein|Schizosaccharomyces pom... 26 7.6
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 156 bits (378), Expect = 4e-39
Identities = 68/87 (78%), Positives = 77/87 (88%)
Frame = +3
Query: 510 EMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQ 689
EMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQ
Sbjct: 291 EMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQ 350
Query: 690 ISNGYTPVLDCHTAHIACKFAEIKEKL 770
IS GY+PVLDCHTAHIACKFAE+ EK+
Sbjct: 351 ISAGYSPVLDCHTAHIACKFAELIEKI 377
Score = 147 bits (357), Expect = 1e-36
Identities = 66/84 (78%), Positives = 73/84 (86%)
Frame = +1
Query: 256 MPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRV 435
MPW++GWQ E K G GK L+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRV
Sbjct: 206 MPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRV 265
Query: 436 ETGVLKPGTIVVFAPANITTEVKS 507
ETGV+KPG IV FAPA +TTEVKS
Sbjct: 266 ETGVIKPGMIVTFAPAGVTTEVKS 289
Score = 131 bits (316), Expect = 1e-31
Identities = 71/134 (52%), Positives = 91/134 (67%), Gaps = 1/134 (0%)
Frame = +2
Query: 2 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIG 181
FEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFEEI KE S++IKK+G
Sbjct: 123 FEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTG--WSQARFEEIVKETSNFIKKVG 180
Query: 182 YNPAAVAFVPISGWHGDNMLEPSTKCLGSRDGRWSVRKAKLTENASLKLSMPSCHLPA-P 358
+NP V FVP+SG+ GDNM+EP+T + G KA + + +L ++ S PA P
Sbjct: 181 FNPKTVPFVPVSGFQGDNMIEPTTN-MPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARP 239
Query: 359 LTSPCVFPCKTYTK 400
P P + K
Sbjct: 240 TDKPLRLPLQDVYK 253
Score = 36.7 bits (81), Expect = 0.004
Identities = 15/24 (62%), Positives = 19/24 (79%)
Frame = +2
Query: 764 KVDRRTGKSTEVNPKSIKSGDAAI 835
K+DRR+GK E +PK +KSGDA I
Sbjct: 376 KIDRRSGKKIEESPKFVKSGDACI 399
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 156 bits (378), Expect = 4e-39
Identities = 68/87 (78%), Positives = 77/87 (88%)
Frame = +3
Query: 510 EMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQ 689
EMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQ
Sbjct: 291 EMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQ 350
Query: 690 ISNGYTPVLDCHTAHIACKFAEIKEKL 770
IS GY+PVLDCHTAHIACKFAE+ EK+
Sbjct: 351 ISAGYSPVLDCHTAHIACKFAELIEKI 377
Score = 147 bits (357), Expect = 1e-36
Identities = 66/84 (78%), Positives = 73/84 (86%)
Frame = +1
Query: 256 MPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRV 435
MPW++GWQ E K G GK L+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRV
Sbjct: 206 MPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRV 265
Query: 436 ETGVLKPGTIVVFAPANITTEVKS 507
ETGV+KPG IV FAPA +TTEVKS
Sbjct: 266 ETGVIKPGMIVTFAPAGVTTEVKS 289
Score = 131 bits (316), Expect = 1e-31
Identities = 71/134 (52%), Positives = 91/134 (67%), Gaps = 1/134 (0%)
Frame = +2
Query: 2 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIG 181
FEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFEEI KE S++IKK+G
Sbjct: 123 FEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTG--WSQARFEEIVKETSNFIKKVG 180
Query: 182 YNPAAVAFVPISGWHGDNMLEPSTKCLGSRDGRWSVRKAKLTENASLKLSMPSCHLPA-P 358
+NP V FVP+SG+ GDNM+EP+T + G KA + + +L ++ S PA P
Sbjct: 181 FNPKTVPFVPVSGFQGDNMIEPTTN-MPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARP 239
Query: 359 LTSPCVFPCKTYTK 400
P P + K
Sbjct: 240 TDKPLRLPLQDVYK 253
Score = 36.7 bits (81), Expect = 0.004
Identities = 15/24 (62%), Positives = 19/24 (79%)
Frame = +2
Query: 764 KVDRRTGKSTEVNPKSIKSGDAAI 835
K+DRR+GK E +PK +KSGDA I
Sbjct: 376 KIDRRSGKKIEESPKFVKSGDACI 399
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 156 bits (378), Expect = 4e-39
Identities = 68/87 (78%), Positives = 77/87 (88%)
Frame = +3
Query: 510 EMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQ 689
EMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQ
Sbjct: 291 EMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQ 350
Query: 690 ISNGYTPVLDCHTAHIACKFAEIKEKL 770
IS GY+PVLDCHTAHIACKFAE+ EK+
Sbjct: 351 ISAGYSPVLDCHTAHIACKFAELIEKI 377
Score = 147 bits (357), Expect = 1e-36
Identities = 66/84 (78%), Positives = 73/84 (86%)
Frame = +1
Query: 256 MPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRV 435
MPW++GWQ E K G GK L+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRV
Sbjct: 206 MPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRV 265
Query: 436 ETGVLKPGTIVVFAPANITTEVKS 507
ETGV+KPG IV FAPA +TTEVKS
Sbjct: 266 ETGVIKPGMIVTFAPAGVTTEVKS 289
Score = 131 bits (316), Expect = 1e-31
Identities = 71/134 (52%), Positives = 91/134 (67%), Gaps = 1/134 (0%)
Frame = +2
Query: 2 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIG 181
FEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFEEI KE S++IKK+G
Sbjct: 123 FEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTG--WSQARFEEIVKETSNFIKKVG 180
Query: 182 YNPAAVAFVPISGWHGDNMLEPSTKCLGSRDGRWSVRKAKLTENASLKLSMPSCHLPA-P 358
+NP V FVP+SG+ GDNM+EP+T + G KA + + +L ++ S PA P
Sbjct: 181 FNPKTVPFVPVSGFQGDNMIEPTTN-MPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARP 239
Query: 359 LTSPCVFPCKTYTK 400
P P + K
Sbjct: 240 TDKPLRLPLQDVYK 253
Score = 36.7 bits (81), Expect = 0.004
Identities = 15/24 (62%), Positives = 19/24 (79%)
Frame = +2
Query: 764 KVDRRTGKSTEVNPKSIKSGDAAI 835
K+DRR+GK E +PK +KSGDA I
Sbjct: 376 KIDRRSGKKIEESPKFVKSGDACI 399
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 77.4 bits (182), Expect = 2e-15
Identities = 40/88 (45%), Positives = 56/88 (63%), Gaps = 1/88 (1%)
Frame = +2
Query: 2 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY-IKKI 178
FE G +NGQTREHA L LG+ +++V VNK+D +SE RF+EIK VS + IK +
Sbjct: 293 FERGFLENGQTREHAYLLRALGISEIVVSVNKLDLMS--WSEDRFQEIKNIVSDFLIKMV 350
Query: 179 GYNPAAVAFVPISGWHGDNMLEPSTKCL 262
G+ + V FVPIS G N+++ + L
Sbjct: 351 GFKTSNVHFVPISAISGTNLIQKDSSDL 378
Score = 45.2 bits (102), Expect = 1e-05
Identities = 23/46 (50%), Positives = 29/46 (63%)
Frame = +1
Query: 307 GKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETG 444
G L+ ALD ++PP +P KPLRL + DVY+ TV GRVE G
Sbjct: 384 GPTLLSALDQLVPPEKPYRKPLRLSIDDVYRSPRSVTV-TGRVEAG 428
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 74.1 bits (174), Expect = 2e-14
Identities = 34/81 (41%), Positives = 54/81 (66%), Gaps = 2/81 (2%)
Frame = +2
Query: 2 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKI- 178
FEAG + GQTREHA+LA T G+ L+V +NKMD +SE R++E ++S +++++
Sbjct: 354 FEAGFERGGQTREHAVLARTQGINHLVVVINKMDEPSVQWSEERYKECVDKLSMFLRRVA 413
Query: 179 GYNPAA-VAFVPISGWHGDNM 238
GYN V ++P+S + G N+
Sbjct: 414 GYNSKTDVKYMPVSAYTGQNV 434
Score = 46.8 bits (106), Expect = 4e-06
Identities = 26/89 (29%), Positives = 45/89 (50%)
Frame = +3
Query: 504 VWEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHP 683
+++ E + ++ GD V V+ +++ GYV +KN P F AQ+ +L P
Sbjct: 513 IYDEADEEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAILELP 570
Query: 684 GQISNGYTPVLDCHTAHIACKFAEIKEKL 770
++ GY+ V+ HTA FA++ KL
Sbjct: 571 SILTTGYSCVMHIHTAVEEVSFAKLLHKL 599
Score = 41.1 bits (92), Expect = 2e-04
Identities = 22/65 (33%), Positives = 35/65 (53%)
Frame = +1
Query: 307 GKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPAN 486
G L+E LD++ R + P +P+ YK +GT+ G++E G +K + V+ P N
Sbjct: 448 GPSLLEYLDSMTHLERKVNAPFIMPIASKYK--DLGTILEGKIEAGSIKKNSNVLVMPIN 505
Query: 487 ITTEV 501
T EV
Sbjct: 506 QTLEV 510
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 49.2 bits (112), Expect = 7e-07
Identities = 24/49 (48%), Positives = 32/49 (65%), Gaps = 1/49 (2%)
Frame = +1
Query: 316 LIEALDAILP-PARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPG 459
L+EA+D+ + P R TD P + ++DV+ I G GTV GRVE G LK G
Sbjct: 234 LMEAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVERGTLKKG 282
Score = 45.2 bits (102), Expect = 1e-05
Identities = 22/46 (47%), Positives = 31/46 (67%)
Frame = +2
Query: 29 QTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY 166
QTREH LLA +GVKQ++V +NK+D EP E E+++ +S Y
Sbjct: 156 QTREHLLLARQVGVKQIVVYINKVDMVEPDMIELVEMEMRELLSEY 201
Score = 29.9 bits (64), Expect = 0.47
Identities = 12/33 (36%), Positives = 21/33 (63%)
Frame = +3
Query: 510 EMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 608
EM + L AV GDN G ++++ ++L+RG +
Sbjct: 302 EMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMI 334
>SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 695
Score = 30.7 bits (66), Expect = 0.27
Identities = 18/67 (26%), Positives = 31/67 (46%)
Frame = +2
Query: 62 LGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNML 241
L + + + KMD E + E +F + + S++K+ F+PISG G N+
Sbjct: 407 LSISSYMFAITKMDEIE--WDENKFINLVNSIQSFLKESCGIIEKSKFIPISGLKGTNLT 464
Query: 242 EPSTKCL 262
S + L
Sbjct: 465 SISQEKL 471
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 30.3 bits (65), Expect = 0.35
Identities = 21/68 (30%), Positives = 30/68 (44%)
Frame = +1
Query: 256 MPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRV 435
+P K V K GK + L E + I PP + PLR L D + G + + R+
Sbjct: 206 IPMSKPILVSSKTGKNVEQILPEIIQKIPPPKGSENAPLRCLLIDSWYNSYQGVIGLVRI 265
Query: 436 ETGVLKPG 459
G +K G
Sbjct: 266 MEGFIKKG 273
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 28.7 bits (61), Expect = 1.1
Identities = 18/53 (33%), Positives = 30/53 (56%)
Frame = -2
Query: 834 MAASPDLMDFGLTSVDLPVRRSTFL*FLQICRQCGQCGNPRLVCNRLRFDQDG 676
++ +PDL D L+SVD P++ +T C + GN L+C+ F++DG
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNT----------CSESGNQYLLCD---FNRDG 66
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 28.7 bits (61), Expect = 1.1
Identities = 21/62 (33%), Positives = 32/62 (51%), Gaps = 1/62 (1%)
Frame = -3
Query: 566 VETYIVSRYSFLESFVVHLPDLTSVVMLAGAKTTM-VPGFNTPVSTLPTGTVPIPPILYT 390
V+T +S+ S + L+S V+L AK T+ V + + VST TGTV +P
Sbjct: 63 VQTIAISQTDNAASCIPSASLLSSSVVLYSAKETVTVSSYWSLVSTSVTGTVYVPYTSSV 122
Query: 389 SC 384
+C
Sbjct: 123 AC 124
>SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 216
Score = 27.9 bits (59), Expect = 1.9
Identities = 21/81 (25%), Positives = 39/81 (48%)
Frame = -2
Query: 528 ELRGASPRLDFSSDVGGGKDNNGTWFQHTSFNSADGHGTNTTDFVYVLQGKTQGLVSGAG 349
E + S +L+F+ VG D+ + + + + DF+ ++GK L +
Sbjct: 113 EHKKRSVKLEFT--VGADADDYSSLQKANNLEPVEADIRRARDFIEEIKGKIYYLQAREA 170
Query: 348 RWQDGIESFNEAFSVSFAFLT 286
R+++ ES NE +FA+LT
Sbjct: 171 RFRNTNESTNERVK-NFAYLT 190
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 4.3
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -3
Query: 593 QFLDGHVLYVETYIVSRYSFLESFV 519
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 26.2 bits (55), Expect = 5.7
Identities = 22/72 (30%), Positives = 25/72 (34%), Gaps = 5/72 (6%)
Frame = -3
Query: 458 PGFNTPVSTLPTGTVPIP-PILYTSCR--GRRRGLSVGRAGGRMASRASMRH--FPSALP 294
P +N P G+VP+P P S R R S GR S H PS LP
Sbjct: 314 PNYNAPFQPAGVGSVPLPAPTSSQSLRLGSLHRSRSPSPRSGRPRRSPSPSHLSIPSTLP 373
Query: 293 SLRSTCHPLNQG 258
P G
Sbjct: 374 PADGVPKPTPDG 385
>SPAC18G6.05c |||translation elongation regulator Gcn1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2670
Score = 25.8 bits (54), Expect = 7.6
Identities = 14/37 (37%), Positives = 19/37 (51%)
Frame = -2
Query: 339 DGIESFNEAFSVSFAFLTLHLPSLEPRHLVEGSNMLS 229
D + EA V F + HLPS +PR V ++LS
Sbjct: 1294 DANDRLREATVVLFGTVAQHLPSNDPRLAVVMDSLLS 1330
>SPCC1442.04c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 409
Score = 25.8 bits (54), Expect = 7.6
Identities = 17/56 (30%), Positives = 27/56 (48%), Gaps = 1/56 (1%)
Frame = -2
Query: 558 LHCLQVQLLGELRGASPRLDFSSDVGGGKDNNGTW-FQHTSFNSADGHGTNTTDFV 394
LH +V+ + E SP L SDV K+N T+ + +FN + N T+ +
Sbjct: 42 LHDGKVEEVVEAINGSPDLLSESDVENAKNNEHTYDIDNKNFNFEPKNSVNKTETI 97
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,856,746
Number of Sequences: 5004
Number of extensions: 87343
Number of successful extensions: 309
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 274
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 304
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 410448950
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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