BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30679
(497 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|... 38 6e-04
SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun... 27 1.6
SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces pom... 27 2.1
SPAC2F3.03c |rpa49|rpa51|DNA-directed RNA polymerase I complex |... 25 4.8
SPAC31G5.15 |||phosphatidylserine decarboxylase |Schizosaccharom... 25 6.3
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 25 6.3
SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase Cdc... 25 6.3
>SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 137
Score = 38.3 bits (85), Expect = 6e-04
Identities = 18/37 (48%), Positives = 25/37 (67%)
Frame = +2
Query: 2 LYSSSFDALKKSLVGVQKYIQATDLSEASQEAVEEKL 112
+YSSS D L+++ G+ IQATD SE + E V EK+
Sbjct: 98 VYSSSKDTLRRAFTGIGTDIQATDFSEVAYETVLEKV 134
>SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 214
Score = 27.1 bits (57), Expect = 1.6
Identities = 10/26 (38%), Positives = 19/26 (73%)
Frame = +2
Query: 14 SFDALKKSLVGVQKYIQATDLSEASQ 91
S +AL++ L G + Y+Q+TD++ S+
Sbjct: 188 SLEALQEELEGFEDYVQSTDIAAMSK 213
>SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 447
Score = 26.6 bits (56), Expect = 2.1
Identities = 22/76 (28%), Positives = 34/76 (44%), Gaps = 3/76 (3%)
Frame = +1
Query: 76 LGSVSGGRRREAPRHRSPINSIYTRARDE---TEPALRHSCPDDTRPRHH*PLSIITKEN 246
LG G ++ E+ R + P S + EP +RHS RP+ H S +T+++
Sbjct: 20 LGEEDGRQKGESSRKKRPPLSRKNPSNVSFWSNEPIIRHSSVKTDRPQFHRADSTVTEQS 79
Query: 247 E*YNMIDFTGGRTSCE 294
+ I F G S E
Sbjct: 80 ---SEILFDGNAESAE 92
>SPAC2F3.03c |rpa49|rpa51|DNA-directed RNA polymerase I complex
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 425
Score = 25.4 bits (53), Expect = 4.8
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = -2
Query: 187 RSVGERVRFRRELVCKCCLLAIGGAELLFDGLLRRFREVGGL 62
RSVG R R++L E+L DGL++RF + G+
Sbjct: 294 RSVGNRETLRKKLA--------DPPEILIDGLIKRFTQTTGI 327
>SPAC31G5.15 |||phosphatidylserine decarboxylase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 980
Score = 25.0 bits (52), Expect = 6.3
Identities = 11/27 (40%), Positives = 16/27 (59%), Gaps = 1/27 (3%)
Frame = +2
Query: 332 CREA-VMRFGLKGGAAVVTIWVAHLCC 409
CR A +R G+K G ++ I V +CC
Sbjct: 7 CRSANTLRKGIKNGKLILKIIVNDVCC 33
>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1334
Score = 25.0 bits (52), Expect = 6.3
Identities = 14/39 (35%), Positives = 17/39 (43%)
Frame = +1
Query: 109 APRHRSPINSIYTRARDETEPALRHSCPDDTRPRHH*PL 225
APR R P ++ T A E P + P TR PL
Sbjct: 24 APRKREPARTVSTPAFMEPAPVSKKPLPPPTRRLPRKPL 62
>SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase
Cdc7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1062
Score = 25.0 bits (52), Expect = 6.3
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +1
Query: 124 SPINSIYTRARDETEPALRHSCPDDTRPRHH*PLSIITKEN 246
SP+++ D ++PAL S P+H LS KEN
Sbjct: 391 SPLSTPLKAQFDPSKPALNRSIDHQKTPQHKRYLSTEFKEN 431
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,989,203
Number of Sequences: 5004
Number of extensions: 39231
Number of successful extensions: 91
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 91
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 91
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 196153982
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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