Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS30611
         (408 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein L30|Sc...   121   4e-29
SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein L30|Sc...   119   2e-28
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha...    29   0.21 
SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce...    26   2.0  
SPAC977.14c |||aldo/keto reductase, unknown biological role|Schi...    25   4.5  
SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor |S...    25   6.0  
SPBPB21E7.09 ||SPAPB21E7.09, SPAPB21E7.09|L-asparaginase |Schizo...    24   7.9  

>SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein
           L30|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 117

 Score =  121 bits (292), Expect = 4e-29
 Identities = 55/83 (66%), Positives = 68/83 (81%)
 Frame = +3

Query: 18  APKMVAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPLRK 197
           AP    +KK KK+ ++INS+LAL MKSGKY LGYK TLKTLR GKAKL++IA NAPPLRK
Sbjct: 8   APVAAVSKKGKKSGDTINSKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAANAPPLRK 67

Query: 198 SEIEYYALLAKTGVHHYSGTTLN 266
           SE+EYYA+L++  VHHYSG  ++
Sbjct: 68  SELEYYAMLSRCSVHHYSGNNID 90



 Score = 45.6 bits (103), Expect = 3e-06
 Identities = 19/29 (65%), Positives = 24/29 (82%)
 Frame = +2

Query: 254 DNIELGTACGKYYRVCTLAITDPGDSDII 340
           +NI+LGTACGK +RV  LA+ D GDSDI+
Sbjct: 87  NNIDLGTACGKLFRVGVLAVIDAGDSDIL 115


>SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein
           L30|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 109

 Score =  119 bits (287), Expect = 2e-28
 Identities = 53/79 (67%), Positives = 66/79 (83%)
 Frame = +3

Query: 30  VAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPLRKSEIE 209
           V  KK KK+ ++IN++LAL MKSGKY LGYK TLKTLR GKAKL++IA N PPLRKSE+E
Sbjct: 4   VVTKKSKKSGDTINAKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAGNCPPLRKSELE 63

Query: 210 YYALLAKTGVHHYSGTTLN 266
           YYA+L+K  VHHY+GT ++
Sbjct: 64  YYAMLSKANVHHYAGTNID 82



 Score = 48.0 bits (109), Expect = 6e-07
 Identities = 21/28 (75%), Positives = 24/28 (85%)
 Frame = +2

Query: 257 NIELGTACGKYYRVCTLAITDPGDSDII 340
           NI+LGTACGK +RV  LAITD GDSDI+
Sbjct: 80  NIDLGTACGKLFRVGVLAITDAGDSDIL 107


>SPAC1834.01 |sup45||translation release factor
           eRF1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 433

 Score = 29.5 bits (63), Expect = 0.21
 Identities = 13/42 (30%), Positives = 24/42 (57%)
 Frame = +3

Query: 42  KQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVI 167
           ++KK I+     ++L   SGKYC G   T+  L++G  + ++
Sbjct: 279 QEKKLIQRFFDEISL--DSGKYCFGVVDTMNALQEGAVETLL 318


>SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 154

 Score = 26.2 bits (55), Expect = 2.0
 Identities = 17/58 (29%), Positives = 32/58 (55%)
 Frame = +3

Query: 18  APKMVAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPL 191
           APK +  KK  KT++  + +  ++        G K+ +K +R+G+  LVI+A +  P+
Sbjct: 33  APKKLN-KKMMKTVKKASKQKHILR-------GVKEVVKAVRKGEKGLVILAGDISPM 82


>SPAC977.14c |||aldo/keto reductase, unknown biological
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 351

 Score = 25.0 bits (52), Expect = 4.5
 Identities = 11/31 (35%), Positives = 20/31 (64%)
 Frame = +3

Query: 150 KAKLVIIAKNAPPLRKSEIEYYALLAKTGVH 242
           ++ +VI++K   P+RK  I+ +  L+  GVH
Sbjct: 87  RSSIVILSKCFFPVRKDLIKIFGDLSSRGVH 117


>SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1052

 Score = 24.6 bits (51), Expect = 6.0
 Identities = 11/30 (36%), Positives = 15/30 (50%)
 Frame = +2

Query: 233 RCSPLQRDNIELGTACGKYYRVCTLAITDP 322
           +C  +QRD+I          +V TL  TDP
Sbjct: 136 KCLEIQRDSIVYAADSAPIIQVSTLETTDP 165


>SPBPB21E7.09 ||SPAPB21E7.09, SPAPB21E7.09|L-asparaginase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 360

 Score = 24.2 bits (50), Expect = 7.9
 Identities = 11/29 (37%), Positives = 19/29 (65%)
 Frame = -3

Query: 235 PVLARRA*YSISDFLSGGAFLAMITSFAL 149
           P+L ++   ++SDF+S  A L  +T FA+
Sbjct: 18  PLLFQKRSSNVSDFISFNASLPNVTIFAM 46


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,480,089
Number of Sequences: 5004
Number of extensions: 25964
Number of successful extensions: 70
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 68
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 70
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 140222766
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)