BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30533 (855 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g02580.1 68417.m00352 NADH-ubiquinone oxidoreductase 24 kDa s... 112 3e-25 At4g19700.1 68417.m02893 expressed protein 31 0.98 At3g59180.1 68416.m06598 hypothetical protein contains a novel d... 30 1.7 At5g28480.1 68418.m03462 hypothetical protein 29 3.0 At2g12100.1 68415.m01300 Ulp1 protease family protein contains P... 29 3.0 At1g45090.1 68414.m05169 Ulp1 protease family protein similar to... 29 3.0 At1g01900.1 68414.m00107 subtilase family protein contains simil... 29 3.0 At3g22550.1 68416.m02849 senescence-associated protein-related s... 29 3.9 At3g02860.2 68416.m00279 expressed protein 29 3.9 At3g02860.1 68416.m00278 expressed protein 29 3.9 At4g17610.1 68417.m02633 tRNA/rRNA methyltransferase (SpoU) fami... 28 6.9 At1g72220.1 68414.m08350 zinc finger (C3HC4-type RING finger) fa... 28 6.9 At5g02170.1 68418.m00138 amino acid transporter family protein b... 28 9.1 At3g29230.1 68416.m03667 pentatricopeptide (PPR) repeat-containi... 28 9.1 >At4g02580.1 68417.m00352 NADH-ubiquinone oxidoreductase 24 kDa subunit, putative similar to NADH-ubiquinone oxidoreductase 24 kDa subunit, mitochondrial precursor (EC 1.6.5.3) (EC 1.6.99.3) (Polypeptide II) (Swiss-Prot:P04394) [Bos taurus] Length = 255 Score = 112 bits (269), Expect = 3e-25 Identities = 52/97 (53%), Positives = 73/97 (75%) Frame = +2 Query: 200 AGQSKESRXFVGHLP*RSQRGAMIPLLDLAQRQSGGWLPISAMHKVAEILNLPKMRVYEV 379 A QSK + + + P ++ A+IPLLDLAQ+Q+GGWLP+SAM+ VA+++ + +RVYEV Sbjct: 52 ANQSKV-KEILSYYPSNYKQSAVIPLLDLAQQQNGGWLPVSAMNAVAKVIEVAPIRVYEV 110 Query: 380 ATFYTMFIRRPIGKYHVQVCTTTPCWLRGSDAILNAI 490 ATFY+MF R +GKYH+ VC TTPC +RGS I +A+ Sbjct: 111 ATFYSMFNRAKVGKYHLLVCGTTPCMIRGSRDIESAL 147 Score = 67.3 bits (157), Expect = 1e-11 Identities = 39/88 (44%), Positives = 50/88 (56%), Gaps = 11/88 (12%) Frame = +1 Query: 532 GKFSVSEVECLGACVNAPMIQVND----------DYYEDLSVEDTKEIISKLKKDEKPKP 681 G FSV E+EC+G CVNAPMI V D +Y+ED++ E EI+ KL+K EKP Sbjct: 162 GLFSVGEMECMGCCVNAPMITVADYSNGSEGYTYNYFEDVTPEKVVEIVEKLRKGEKPPH 221 Query: 682 GPRS-GRFASEPLGGLTSLTEEPTGPGF 762 G ++ R P GG +L EP P F Sbjct: 222 GTQNPKRIKCGPEGGNKTLLGEPKPPQF 249 Score = 45.6 bits (103), Expect = 4e-05 Identities = 16/40 (40%), Positives = 29/40 (72%) Frame = +3 Query: 141 SLFVHRDTPEDNPSIPFEFSQANQKRVEXLLAIYPEGHSE 260 +L H D+P++ P +P+EFS+ANQ +V+ +L+ YP + + Sbjct: 31 ALNYHLDSPDNKPDLPWEFSEANQSKVKEILSYYPSNYKQ 70 >At4g19700.1 68417.m02893 expressed protein Length = 304 Score = 31.1 bits (67), Expect = 0.98 Identities = 22/76 (28%), Positives = 32/76 (42%) Frame = +3 Query: 120 TASRQHDSLFVHRDTPEDNPSIPFEFSQANQKRVEXLLAIYPEGHSEVP*FLYWIWHNDR 299 + RQ D F + P S+ F+ S + VE + I + SE+ F+ R Sbjct: 76 STKRQRDFQFSDSNAPVKRRSVAFDSSSPSLINVELVSQIQNQQQSEIDRFVAQQTEKLR 135 Query: 300 VEAGYRSQLCTRWLRS 347 +E R Q TR L S Sbjct: 136 IEIEARQQTQTRMLAS 151 >At3g59180.1 68416.m06598 hypothetical protein contains a novel domain with similarity to F-box domain; Length = 475 Score = 30.3 bits (65), Expect = 1.7 Identities = 18/51 (35%), Positives = 27/51 (52%), Gaps = 1/51 (1%) Frame = +1 Query: 517 GNSPCGKFSVS-EVECLGACVNAPMIQVNDDYYEDLSVEDTKEIISKLKKD 666 GN+P +FS+ +V C ACV+ +I V D DL + + E KL + Sbjct: 50 GNAPLNRFSLKCKVRCDSACVDGWIINVLDRGVLDLDLNISSESDYKLPSE 100 >At5g28480.1 68418.m03462 hypothetical protein Length = 1230 Score = 29.5 bits (63), Expect = 3.0 Identities = 14/41 (34%), Positives = 23/41 (56%) Frame = +1 Query: 592 QVNDDYYEDLSVEDTKEIISKLKKDEKPKPGPRSGRFASEP 714 Q+ D +EDL V +EI + L+ ++ + GPRS + P Sbjct: 494 QLLDKKFEDLKVFVREEIRAALQSQDRKEEGPRSSHKSDSP 534 >At2g12100.1 68415.m01300 Ulp1 protease family protein contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain; similar to At5g28270, At2g05450, At1g45090, At2g16180, At2g06750 Length = 1224 Score = 29.5 bits (63), Expect = 3.0 Identities = 14/41 (34%), Positives = 23/41 (56%) Frame = +1 Query: 592 QVNDDYYEDLSVEDTKEIISKLKKDEKPKPGPRSGRFASEP 714 Q+ D +EDL V +EI + L+ ++ + GPRS + P Sbjct: 507 QLLDKKFEDLKVFVREEIRAALQSQDRKEEGPRSSHKSDSP 547 >At1g45090.1 68414.m05169 Ulp1 protease family protein similar to At5g28270, At2g12100, At2g05450, At2g16180, At2g06750; contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain Length = 1210 Score = 29.5 bits (63), Expect = 3.0 Identities = 14/41 (34%), Positives = 23/41 (56%) Frame = +1 Query: 592 QVNDDYYEDLSVEDTKEIISKLKKDEKPKPGPRSGRFASEP 714 Q+ D +EDL V +EI + L+ ++ + GPRS + P Sbjct: 498 QLLDKKFEDLKVFVREEIRAALQSQDRKEEGPRSSHKSDSP 538 >At1g01900.1 68414.m00107 subtilase family protein contains similarity to cucumisin-like serine protease GB:AAC18851 GI:3176874 from [Arabidopsis thaliana] Length = 774 Score = 29.5 bits (63), Expect = 3.0 Identities = 14/58 (24%), Positives = 30/58 (51%) Frame = -1 Query: 681 RFRFLILL*FADNFFCIFYRQVLIVIIIDLYHWGVHTSSQTFNL*NGKLSTRTVASNL 508 RF ++L F+DN +F+R ++ + + V + QT+ + ST+ + ++L Sbjct: 3 RFLLMLLFPFSDNRPMMFFRSFIVFFFLIFFASNVSSRKQTYVIHTVTTSTKHIVTSL 60 >At3g22550.1 68416.m02849 senescence-associated protein-related similar to senescence-associated protein SAG102 (GI:22331931) [Arabidopsis thaliana] Length = 267 Score = 29.1 bits (62), Expect = 3.9 Identities = 11/18 (61%), Positives = 14/18 (77%) Frame = +1 Query: 643 IISKLKKDEKPKPGPRSG 696 I+ L +DE P+PGPRSG Sbjct: 93 IVDSLIQDETPEPGPRSG 110 >At3g02860.2 68416.m00279 expressed protein Length = 313 Score = 29.1 bits (62), Expect = 3.9 Identities = 15/55 (27%), Positives = 28/55 (50%), Gaps = 2/55 (3%) Frame = +1 Query: 511 VGGNSPCGKFSVSEVECLGACVNAPMIQVNDDY--YEDLSVEDTKEIISKLKKDE 669 V GN P G F E + L + + D+Y +E L +D + + S+++++E Sbjct: 182 VNGNLPTGFFDNKEADLLAHGIKLVKPDIKDEYKEFEKLIQDDLQVVDSRMEEEE 236 >At3g02860.1 68416.m00278 expressed protein Length = 312 Score = 29.1 bits (62), Expect = 3.9 Identities = 15/55 (27%), Positives = 28/55 (50%), Gaps = 2/55 (3%) Frame = +1 Query: 511 VGGNSPCGKFSVSEVECLGACVNAPMIQVNDDY--YEDLSVEDTKEIISKLKKDE 669 V GN P G F E + L + + D+Y +E L +D + + S+++++E Sbjct: 181 VNGNLPTGFFDNKEADLLAHGIKLVKPDIKDEYKEFEKLIQDDLQVVDSRMEEEE 235 >At4g17610.1 68417.m02633 tRNA/rRNA methyltransferase (SpoU) family protein similar to TAR RNA loop binding protein [Homo sapiens] GI:1184692; contains Pfam profile PF00588: SpoU rRNA Methylase (RNA methyltransferase, TrmH) family Length = 1850 Score = 28.3 bits (60), Expect = 6.9 Identities = 15/48 (31%), Positives = 25/48 (52%), Gaps = 4/48 (8%) Frame = +1 Query: 547 SEVECLGACVNAPMIQVNDDYYEDLSVEDTKEIIS----KLKKDEKPK 678 SE EC+ C+ ++ +D EDL K++++ K DE+PK Sbjct: 1572 SEFECVPTCLMDNVLSFLNDVREDLRASMAKDVVTIKNEGFKIDEEPK 1619 >At1g72220.1 68414.m08350 zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger); similar to GI:4928397 from [Arabidopsis thaliana] (Plant Mol. Biol. 40 (4), 579-590 (1999)) Length = 413 Score = 28.3 bits (60), Expect = 6.9 Identities = 11/26 (42%), Positives = 18/26 (69%) Frame = +2 Query: 740 KNPQDLASSTRRTQGVNLVLSCVNRN 817 K+ D++SST +T G + +SC N+N Sbjct: 381 KSQSDISSSTLKTNGSSSSVSCFNKN 406 >At5g02170.1 68418.m00138 amino acid transporter family protein belongs to INTERPRO:IPR002422 amino acid/polyamine transporter, family II Length = 526 Score = 27.9 bits (59), Expect = 9.1 Identities = 17/51 (33%), Positives = 26/51 (50%) Frame = +1 Query: 646 ISKLKKDEKPKPGPRSGRFASEPLGGLTSLTEEPTGPGFEYKTHSRRKSGV 798 +S+ + ++ P P PRS R + + L G+T PT F RR+S V Sbjct: 36 LSRNRSEDVPVPWPRSYRQSMDLLTGVT----PPTSTSFVSSFRQRRQSSV 82 >At3g29230.1 68416.m03667 pentatricopeptide (PPR) repeat-containing protein contains INTERPRO:IPR002885 PPR repeats Length = 600 Score = 27.9 bits (59), Expect = 9.1 Identities = 13/36 (36%), Positives = 19/36 (52%) Frame = -2 Query: 362 FLEDLRSQPPCA*LRSVASLHSVVVPDPVKESWHLA 255 F E L+ P CA L V LH+ ++ + E H+A Sbjct: 19 FEERLQDLPKCANLNQVKQLHAQIIRRNLHEDLHIA 54 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 19,211,499 Number of Sequences: 28952 Number of extensions: 413718 Number of successful extensions: 1224 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 1190 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1223 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1989897600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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