BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30479 (373 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g10700.1 68414.m01217 ribose-phosphate pyrophosphokinase 3 / ... 30 0.56 At5g35380.1 68418.m04205 protein kinase family protein contains ... 29 1.3 At5g15630.1 68418.m01829 phytochelatin synthetase family protein... 29 1.3 At4g05390.1 68417.m00821 ferredoxin--NADP(+) reductase, putative... 27 3.0 At3g11960.2 68416.m01476 cleavage and polyadenylation specificit... 27 5.3 At3g11960.1 68416.m01475 cleavage and polyadenylation specificit... 27 5.3 At2g13610.1 68415.m01500 ABC transporter family protein 27 5.3 At1g16220.1 68414.m01942 protein phosphatase 2C family protein /... 26 9.2 >At1g10700.1 68414.m01217 ribose-phosphate pyrophosphokinase 3 / phosphoribosyl diphosphate synthetase 3 (PRS3) nearly identical to phosphoribosyl diphosphate synthase GI:4902470 from [Arabidopsis thaliana] Length = 411 Score = 29.9 bits (64), Expect = 0.56 Identities = 15/39 (38%), Positives = 25/39 (64%) Frame = -2 Query: 276 SSICHSVTHTSSPSILDTPLRSQTSTCIHHIMRSIKHHS 160 SS S++ +SSPS L+ S ++ CI +RS+++HS Sbjct: 21 SSTSSSLSSSSSPSFLNFKTASVSNRCIKCGVRSLENHS 59 >At5g35380.1 68418.m04205 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 731 Score = 28.7 bits (61), Expect = 1.3 Identities = 13/25 (52%), Positives = 17/25 (68%) Frame = -2 Query: 219 LRSQTSTCIHHIMRSIKHHSHIRPS 145 LRS TS+ H M S++HHSH + S Sbjct: 153 LRSATSSPPHSNMPSMRHHSHAQTS 177 >At5g15630.1 68418.m01829 phytochelatin synthetase family protein / COBRA cell expansion protein COBL4 similar to phytochelatin synthetase [Hordeum vulgare subsp. vulgare] GI:29570314; identified in Roudier, et al, Plant Phys. (2002) 130:538-548 (PMID:12376623); contains Pfam profile PF04833: Phytochelatin synthetase-like conserved region Length = 431 Score = 28.7 bits (61), Expect = 1.3 Identities = 15/54 (27%), Positives = 26/54 (48%), Gaps = 2/54 (3%) Frame = -2 Query: 306 PSCSCFVRRRSSICHSVTHTSSPSILDTPLRSQTS--TCIHHIMRSIKHHSHIR 151 PSC+C + S + + + L+TP + T C HH M ++ H H++ Sbjct: 233 PSCACGCENKKSCVKADSKILTKKGLNTPKKDNTPLLQCTHH-MCPVRVHWHVK 285 >At4g05390.1 68417.m00821 ferredoxin--NADP(+) reductase, putative / adrenodoxin reductase, putative strong similarity to SP|P41345 Ferredoxin--NADP reductase, root isozyme, chloroplast precursor (EC 1.18.1.2) (FNR) {Oryza sativa}, ferredoxin-NADP reductase precursor [Zea mays] GI:500751 Length = 378 Score = 27.5 bits (58), Expect = 3.0 Identities = 13/29 (44%), Positives = 19/29 (65%) Frame = -2 Query: 303 SCSCFVRRRSSICHSVTHTSSPSILDTPL 217 S S V++RS+IC S+ +S +L TPL Sbjct: 49 SRSLGVKKRSTICMSLQQSSKSKVLVTPL 77 >At3g11960.2 68416.m01476 cleavage and polyadenylation specificity factor (CPSF) A subunit C-terminal domain-containing protein similar to Splicing factor 3B subunit 3 (Spliceosome associatedprotein 130) (SAP 130) (SF3b130) (Pre-mRNA splicing factor SF3b 130kDa subunit) (SP:Q15393) [Homo sapiens]; contains Pfam PF03178 : CPSF A subunit region Length = 1329 Score = 26.6 bits (56), Expect = 5.3 Identities = 13/55 (23%), Positives = 28/55 (50%), Gaps = 1/55 (1%) Frame = -2 Query: 237 SILDTPLRSQTST-CIHHIMRSIKHHSHIRPSCT*VCPQSYLFITLTSYNIEKVI 76 ++ D P QT+ + + S + +H P C+ CPQ LF++ ++ +++ Sbjct: 784 ALSDRPWLLQTARQSLSYTSISFQPSTHATPVCSFECPQGILFVSENCLHLVEMV 838 >At3g11960.1 68416.m01475 cleavage and polyadenylation specificity factor (CPSF) A subunit C-terminal domain-containing protein similar to Splicing factor 3B subunit 3 (Spliceosome associatedprotein 130) (SAP 130) (SF3b130) (Pre-mRNA splicing factor SF3b 130kDa subunit) (SP:Q15393) [Homo sapiens]; contains Pfam PF03178 : CPSF A subunit region Length = 1379 Score = 26.6 bits (56), Expect = 5.3 Identities = 13/55 (23%), Positives = 28/55 (50%), Gaps = 1/55 (1%) Frame = -2 Query: 237 SILDTPLRSQTST-CIHHIMRSIKHHSHIRPSCT*VCPQSYLFITLTSYNIEKVI 76 ++ D P QT+ + + S + +H P C+ CPQ LF++ ++ +++ Sbjct: 842 ALSDRPWLLQTARQSLSYTSISFQPSTHATPVCSFECPQGILFVSENCLHLVEMV 896 >At2g13610.1 68415.m01500 ABC transporter family protein Length = 649 Score = 26.6 bits (56), Expect = 5.3 Identities = 17/45 (37%), Positives = 20/45 (44%) Frame = -2 Query: 285 RRRSSICHSVTHTSSPSILDTPLRSQTSTCIHHIMRSIKHHSHIR 151 RRR SI V H ILD P ST I+ +KH + R Sbjct: 188 RRRVSIGVEVIHDPKVLILDEPTSGLDSTSALLIIDMLKHMAETR 232 >At1g16220.1 68414.m01942 protein phosphatase 2C family protein / PP2C family protein similar to protein phosphatase-2C; PP2C (GI:3643088) [Mesembryanthemum crystallinum]; contains Pfam PF00481 : Protein phosphatase 2C domain; Length = 491 Score = 25.8 bits (54), Expect = 9.2 Identities = 21/76 (27%), Positives = 35/76 (46%) Frame = -2 Query: 246 SSPSILDTPLRSQTSTCIHHIMRSIKHHSHIRPSCT*VCPQSYLFITLTSYNIEKVIQKV 67 S+PS DT R+ T + +K+ + C VC LF+ TS + + Sbjct: 338 SAPS-RDTAARAVVDTAVR--AWRLKYPTSKNDDCAVVC----LFLEDTSAGGTVEVSET 390 Query: 66 INHS*TKQLTTITLTS 19 +NHS + ++T+TS Sbjct: 391 VNHSHEESTESVTITS 406 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 6,407,685 Number of Sequences: 28952 Number of extensions: 119182 Number of successful extensions: 348 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 342 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 348 length of database: 12,070,560 effective HSP length: 73 effective length of database: 9,957,064 effective search space used: 497853200 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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