BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30472 (697 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_23747| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.3 SB_28262| Best HMM Match : Sarcoglycan_1 (HMM E-Value=0.95) 28 8.3 >SB_23747| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 853 Score = 28.3 bits (60), Expect = 6.3 Identities = 27/102 (26%), Positives = 47/102 (46%) Frame = +3 Query: 258 HRLVETYAFGDKKSYTLYLKDYMKKLVAKLEEKAPDQVEVFKTNMNKVMKDILGRFKELQ 437 H+LVE +++ ++ +D LVA Q VF+ + N V K G K Sbjct: 734 HKLVEKRLVSNQQQRSIRFRDQRPHLVA--------QGVVFEPDANDVSK---GTLKVTG 782 Query: 438 FFTGESMDCDGMVAMMEYRDFDGTKYQS*CFSNMV*KKRNSK 563 F G+++ + +V + Y DF ++ S S + KK ++K Sbjct: 783 FVRGQTLSANRLVHLAGYGDFQISQIDSVADSFSI-KKSSTK 823 >SB_28262| Best HMM Match : Sarcoglycan_1 (HMM E-Value=0.95) Length = 255 Score = 27.9 bits (59), Expect = 8.3 Identities = 12/32 (37%), Positives = 19/32 (59%) Frame = +3 Query: 576 NNSIIPNWALDTFVFLSIYQSCPHPGRNWLLS 671 +NSI N + FVF ++++SC R+W S Sbjct: 169 SNSIPSNTIIINFVFAALHRSCLRQKRSWNFS 200 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 20,627,967 Number of Sequences: 59808 Number of extensions: 394419 Number of successful extensions: 826 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 765 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 826 length of database: 16,821,457 effective HSP length: 80 effective length of database: 12,036,817 effective search space used: 1817559367 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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