Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30467
(587 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing ... 32 0.25
At4g31580.1 68417.m04485 splicing factor RSZp22 (RSZP22) / 9G8-l... 31 0.57
At2g24590.1 68415.m02936 splicing factor, putative similar to to... 30 1.00
At4g00980.1 68417.m00132 zinc knuckle (CCHC-type) family protein... 30 1.3
At2g46180.1 68415.m05742 intracellular protein transport protein... 28 4.0
At2g17870.1 68415.m02070 cold-shock DNA-binding family protein c... 28 4.0
At5g63920.1 68418.m08026 DNA topoisomerase III alpha, putative s... 27 7.0
At3g53500.2 68416.m05907 zinc knuckle (CCHC-type) family protein... 27 7.0
At3g53500.1 68416.m05906 zinc knuckle (CCHC-type) family protein... 27 7.0
At2g37340.3 68415.m04580 splicing factor RSZ33 (RSZ33) nearly id... 27 7.0
At2g37340.2 68415.m04579 splicing factor RSZ33 (RSZ33) nearly id... 27 7.0
At2g37340.1 68415.m04581 splicing factor RSZ33 (RSZ33) nearly id... 27 7.0
At5g41140.1 68418.m05001 expressed protein 27 9.3
At5g22040.1 68418.m02566 expressed protein 27 9.3
At1g54590.1 68414.m06226 splicing factor Prp18 family protein co... 27 9.3
At1g23860.2 68414.m03010 splicing factor RSZp21 (RSZP21) / 9G8-l... 27 9.3
At1g23860.1 68414.m03009 splicing factor RSZp21 (RSZP21) / 9G8-l... 27 9.3
>At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing
protein low similarity to nucleolar phosphoprotein
(Nopp52), Tetrahymena thermophila, EMBL:TT51555;
contains InterPro entry IPR000504: RNA-binding region
RNP-1 (RNA recognition motif) (RRM)
Length = 597
Score = 32.3 bits (70), Expect = 0.25
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = +2
Query: 494 VQALHCDSCGEKGHLKSMCRNRKGNQ 571
++ +C CGEKGHL S C N+ Q
Sbjct: 571 IKKRNCYECGEKGHLSSACPNKLQKQ 596
Score = 30.3 bits (65), Expect = 1.00
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = +2
Query: 482 GSVSVQALHCDSCGEKGHLKSMC 550
G+ V+ +C CGEKGHL + C
Sbjct: 472 GTGKVKRRNCYECGEKGHLSTAC 494
Score = 27.9 bits (59), Expect = 5.3
Identities = 11/22 (50%), Positives = 13/22 (59%)
Frame = +2
Query: 485 SVSVQALHCDSCGEKGHLKSMC 550
S V+ C CGEKGHL + C
Sbjct: 389 SSKVKRRVCYECGEKGHLSTAC 410
>At4g31580.1 68417.m04485 splicing factor RSZp22 (RSZP22) / 9G8-like
SR protein (SRZ22) identical to RSZp22 protein
[Arabidopsis thaliana] gi|2582645|emb|CAA05352, 9G8-like
SR protein [Arabidopsis thaliana] GI:3435094; contains
Pfam profiles PF00076: RNA recognition motif. (a.k.a.
RRM, RBD, or RNP domain) and PF00098: Zinc knuckle;
identical to cDNA 9G8-like SR protein (SRZ22) GI:3435093
Length = 200
Score = 31.1 bits (67), Expect = 0.57
Identities = 12/21 (57%), Positives = 12/21 (57%)
Frame = +2
Query: 503 LHCDSCGEKGHLKSMCRNRKG 565
L C CGE GH CRNR G
Sbjct: 99 LKCYECGETGHFARECRNRGG 119
>At2g24590.1 68415.m02936 splicing factor, putative similar to to
RSZp22 protein [Arabidopsis thaliana]
gi|2582645|emb|CAA05352
Length = 196
Score = 30.3 bits (65), Expect = 1.00
Identities = 14/36 (38%), Positives = 16/36 (44%)
Frame = +2
Query: 461 RQAGPCGGSVSVQALHCDSCGEKGHLKSMCRNRKGN 568
R G G L C CGE GH CR+R G+
Sbjct: 82 RGGGDGGRGRGGSDLKCYECGESGHFARECRSRGGS 117
>At4g00980.1 68417.m00132 zinc knuckle (CCHC-type) family protein
contains Pfam domain, PF00098: Zinc knuckle
Length = 488
Score = 29.9 bits (64), Expect = 1.3
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = +2
Query: 509 CDSCGEKGHLKSMCRNRKGNQ 571
CD+CG KGHL C K ++
Sbjct: 438 CDNCGRKGHLAKHCWGSKSDE 458
>At2g46180.1 68415.m05742 intracellular protein transport protein
USO1-related similar to Intracellular protein transport
protein USO1 (Swiss-Prot:P25386) [Saccharomyces
cerevisiae]
Length = 725
Score = 28.3 bits (60), Expect = 4.0
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +1
Query: 82 LEPERSEIAERHIFRQRKQQNNESIRSYLQSLK 180
LE ER + A I Q +++ NE+ + LQSL+
Sbjct: 206 LEKEREKSANLQILLQEERKQNETFKEELQSLR 238
>At2g17870.1 68415.m02070 cold-shock DNA-binding family protein
contains Pfam domains, PF00313: 'Cold-shock' DNA-binding
domain and PF00098: Zinc knuckle
Length = 301
Score = 28.3 bits (60), Expect = 4.0
Identities = 11/19 (57%), Positives = 11/19 (57%)
Frame = +2
Query: 509 CDSCGEKGHLKSMCRNRKG 565
C SCGE GHL CR G
Sbjct: 163 CYSCGEVGHLAKDCRGGSG 181
>At5g63920.1 68418.m08026 DNA topoisomerase III alpha, putative
similar to Swiss-Prot:Q9NG98 DNA topoisomerase III alpha
[Drosophila melanogaster]
Length = 926
Score = 27.5 bits (58), Expect = 7.0
Identities = 9/25 (36%), Positives = 13/25 (52%)
Frame = +2
Query: 494 VQALHCDSCGEKGHLKSMCRNRKGN 568
V + C SCG+ H + C NR +
Sbjct: 897 VSGIRCFSCGDPSHFANACPNRNNS 921
>At3g53500.2 68416.m05907 zinc knuckle (CCHC-type) family protein
contains Pfam domain PF00098: Zinc knuckle
Length = 284
Score = 27.5 bits (58), Expect = 7.0
Identities = 8/16 (50%), Positives = 12/16 (75%)
Frame = +2
Query: 509 CDSCGEKGHLKSMCRN 556
C CGE+GH++ C+N
Sbjct: 123 CYRCGERGHIERNCKN 138
>At3g53500.1 68416.m05906 zinc knuckle (CCHC-type) family protein
contains Pfam domain PF00098: Zinc knuckle
Length = 243
Score = 27.5 bits (58), Expect = 7.0
Identities = 8/16 (50%), Positives = 12/16 (75%)
Frame = +2
Query: 509 CDSCGEKGHLKSMCRN 556
C CGE+GH++ C+N
Sbjct: 82 CYRCGERGHIERNCKN 97
>At2g37340.3 68415.m04580 splicing factor RSZ33 (RSZ33) nearly
identical to splicing factor RSZ33 [Arabidopsis
thaliana] GI:9843663; contains Pfam profiles PF00076:
RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain),
PF00098: Zinc knuckle
Length = 249
Score = 27.5 bits (58), Expect = 7.0
Identities = 8/16 (50%), Positives = 12/16 (75%)
Frame = +2
Query: 509 CDSCGEKGHLKSMCRN 556
C CGE+GH++ C+N
Sbjct: 82 CYRCGERGHIERNCKN 97
>At2g37340.2 68415.m04579 splicing factor RSZ33 (RSZ33) nearly
identical to splicing factor RSZ33 [Arabidopsis
thaliana] GI:9843663; contains Pfam profiles PF00076:
RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain),
PF00098: Zinc knuckle
Length = 260
Score = 27.5 bits (58), Expect = 7.0
Identities = 8/16 (50%), Positives = 12/16 (75%)
Frame = +2
Query: 509 CDSCGEKGHLKSMCRN 556
C CGE+GH++ C+N
Sbjct: 93 CYRCGERGHIERNCKN 108
>At2g37340.1 68415.m04581 splicing factor RSZ33 (RSZ33) nearly
identical to splicing factor RSZ33 [Arabidopsis
thaliana] GI:9843663; contains Pfam profiles PF00076:
RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain),
PF00098: Zinc knuckle
Length = 290
Score = 27.5 bits (58), Expect = 7.0
Identities = 8/16 (50%), Positives = 12/16 (75%)
Frame = +2
Query: 509 CDSCGEKGHLKSMCRN 556
C CGE+GH++ C+N
Sbjct: 123 CYRCGERGHIERNCKN 138
>At5g41140.1 68418.m05001 expressed protein
Length = 983
Score = 27.1 bits (57), Expect = 9.3
Identities = 10/34 (29%), Positives = 20/34 (58%)
Frame = +1
Query: 19 CAPDLPEEKDFDILVKLVKDHLEPERSEIAERHI 120
C + +++D L +LVK H++ + + + ER I
Sbjct: 448 CTSETDDDEDQKALDELVKGHMDAKEAHVLERRI 481
>At5g22040.1 68418.m02566 expressed protein
Length = 284
Score = 27.1 bits (57), Expect = 9.3
Identities = 17/48 (35%), Positives = 26/48 (54%)
Frame = +1
Query: 37 EEKDFDILVKLVKDHLEPERSEIAERHIFRQRKQQNNESIRSYLQSLK 180
E+K D + +L KD +E E +EIA RH R++ N L +L+
Sbjct: 128 EQKFMDDITRLAKDQIEAEDAEIA-RH--REKINTINARYEEQLATLR 172
>At1g54590.1 68414.m06226 splicing factor Prp18 family protein
contains Pfam profile: PF02840 Prp18 domain
Length = 256
Score = 27.1 bits (57), Expect = 9.3
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = +1
Query: 142 NNESIRSYLQSLKHLAKTC 198
NNE+ R YLQS+K L C
Sbjct: 184 NNETTRKYLQSVKRLMTFC 202
>At1g23860.2 68414.m03010 splicing factor RSZp21 (RSZP21) / 9G8-like
SR protein (SRZ21) nearly identical to 9G8-like splicing
factor SRZ21 [Arabidopsis thaliana] GI:3435096, RSZp21
protein [Arabidopsis thaliana] GI:2582643
Length = 187
Score = 27.1 bits (57), Expect = 9.3
Identities = 11/35 (31%), Positives = 16/35 (45%)
Frame = +2
Query: 464 QAGPCGGSVSVQALHCDSCGEKGHLKSMCRNRKGN 568
+ G G ++ C CGE GH CR +G+
Sbjct: 76 RGGGGGRRGGIEDSKCYECGELGHFARECRRGRGS 110
>At1g23860.1 68414.m03009 splicing factor RSZp21 (RSZP21) / 9G8-like
SR protein (SRZ21) nearly identical to 9G8-like splicing
factor SRZ21 [Arabidopsis thaliana] GI:3435096, RSZp21
protein [Arabidopsis thaliana] GI:2582643
Length = 187
Score = 27.1 bits (57), Expect = 9.3
Identities = 11/35 (31%), Positives = 16/35 (45%)
Frame = +2
Query: 464 QAGPCGGSVSVQALHCDSCGEKGHLKSMCRNRKGN 568
+ G G ++ C CGE GH CR +G+
Sbjct: 76 RGGGGGRRGGIEDSKCYECGELGHFARECRRGRGS 110
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,164,878
Number of Sequences: 28952
Number of extensions: 173463
Number of successful extensions: 613
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 581
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 613
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1161268208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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