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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS30466
         (767 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS...    32   0.37 
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote...    32   0.37 
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS...    32   0.48 
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote...    31   0.64 
At4g25434.1 68417.m03661 MutT/nudix family protein similar to SP...    29   2.6  
At5g51230.2 68418.m06353 embryonic flower 2 (EMF2) identical to ...    29   3.4  
At5g51230.1 68418.m06352 embryonic flower 2 (EMF2) identical to ...    29   3.4  
At1g65710.1 68414.m07458 expressed protein ; expression supporte...    29   4.5  
At4g14830.1 68417.m02280 expressed protein                             28   6.0  
At3g45690.1 68416.m04938 proton-dependent oligopeptide transport...    28   6.0  

>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
           (HSP17.4-CI) identical to 17.4 kDa class I heat shock
           protein SP:P19036 from [Arabidopsis thaliana]
          Length = 156

 Score = 32.3 bits (70), Expect = 0.37
 Identities = 15/34 (44%), Positives = 24/34 (70%)
 Frame = +3

Query: 408 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAP 509
           S +F+RR+ LPE A  E V++ +  +GVL++T P
Sbjct: 108 SGKFMRRFRLPENAKVEEVKASM-ENGVLSVTVP 140


>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
           (HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
           crystallin family; identified in Scharf, K-D., et al,
           Cell Stress & Chaperones (2001) 6: 225-237.
          Length = 153

 Score = 32.3 bits (70), Expect = 0.37
 Identities = 15/34 (44%), Positives = 22/34 (64%)
 Frame = +3

Query: 408 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAP 509
           S QF RR+ LPE    + V++ +  +GVLT+T P
Sbjct: 105 SGQFTRRFRLPENVKMDQVKAAM-ENGVLTVTVP 137


>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
           (HSP18.1-CI) identical to 18.2 kDa class I heat shock
           protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
           contains Pfam profile: PF00011 Hsp20/alpha crystallin
           family
          Length = 161

 Score = 31.9 bits (69), Expect = 0.48
 Identities = 15/34 (44%), Positives = 23/34 (67%)
 Frame = +3

Query: 408 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAP 509
           S +F+RR+ LPE A  E V++ +  +GVLT+  P
Sbjct: 111 SGKFMRRFRLPENAKMEEVKATM-ENGVLTVVVP 143


>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
           (HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
           heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
           (GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
           17 (19), 7995 (1989))
          Length = 157

 Score = 31.5 bits (68), Expect = 0.64
 Identities = 14/34 (41%), Positives = 23/34 (67%)
 Frame = +3

Query: 408 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAP 509
           S +F RR+ LPE A  E +++ +  +GVL++T P
Sbjct: 109 SGKFTRRFRLPENAKMEEIKASM-ENGVLSVTVP 141


>At4g25434.1 68417.m03661 MutT/nudix family protein similar to
           SP|P53370 Nucleoside diphosphate-linked moiety X motif 6
           {Homo sapiens}; contains Pfam profile PF00293: NUDIX
           domain
          Length = 304

 Score = 29.5 bits (63), Expect = 2.6
 Identities = 14/36 (38%), Positives = 18/36 (50%)
 Frame = -1

Query: 659 EQIKMHHCKPPYLFSASWVPSLWSLISLRNGTGSVR 552
           E  + HH +P YL    W+P   S I L N +  VR
Sbjct: 80  EGFRYHHAEPTYLMLVYWIPEAESTIPL-NASHRVR 114


>At5g51230.2 68418.m06353 embryonic flower 2 (EMF2) identical to
           embryonic flower 2 [Arabidopsis thaliana] GI:14276050;
           supporting cDNA gi|14276049|dbj|AB053171.1|
          Length = 626

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 12/26 (46%), Positives = 16/26 (61%)
 Frame = -2

Query: 562 VLCDGHLSLSLDGVGYLPGAVMVRTP 485
           VL DGH+  + +    L G +MVRTP
Sbjct: 565 VLADGHIPWACEAFSRLHGPIMVRTP 590


>At5g51230.1 68418.m06352 embryonic flower 2 (EMF2) identical to
           embryonic flower 2 [Arabidopsis thaliana] GI:14276050;
           supporting cDNA gi|14276049|dbj|AB053171.1|
          Length = 631

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 12/26 (46%), Positives = 16/26 (61%)
 Frame = -2

Query: 562 VLCDGHLSLSLDGVGYLPGAVMVRTP 485
           VL DGH+  + +    L G +MVRTP
Sbjct: 570 VLADGHIPWACEAFSRLHGPIMVRTP 595


>At1g65710.1 68414.m07458 expressed protein ; expression supported
           by MPSS
          Length = 455

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 15/29 (51%), Positives = 15/29 (51%)
 Frame = +3

Query: 513 RYPTPSRERERCPSHRTGPVSQGDQGPER 599
           R  TPSRERER  S   G V  G  G  R
Sbjct: 169 RRRTPSRERERSGSRERGNVGGGGGGSRR 197


>At4g14830.1 68417.m02280 expressed protein
          Length = 152

 Score = 28.3 bits (60), Expect = 6.0
 Identities = 13/30 (43%), Positives = 15/30 (50%)
 Frame = +3

Query: 420 VRRYALPEGAAPETVESRLSSDGVLTITAP 509
           V R+ LPE   PE V      DG L +T P
Sbjct: 109 VWRFRLPESTRPELVTVDCDGDGELIVTVP 138


>At3g45690.1 68416.m04938 proton-dependent oligopeptide transport
           (POT) family protein contains Pfam profile: PF00854 POT
           family
          Length = 516

 Score = 28.3 bits (60), Expect = 6.0
 Identities = 12/29 (41%), Positives = 19/29 (65%)
 Frame = -2

Query: 580 PCETGPVLCDGHLSLSLDGVGYLPGAVMV 494
           PCETG +LC+    L L G+ Y+  A+++
Sbjct: 120 PCETGSILCESPSKLQL-GILYIALALVI 147


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,914,654
Number of Sequences: 28952
Number of extensions: 334945
Number of successful extensions: 934
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 905
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 934
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1721869952
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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