BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30466 (767 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 32 0.37 At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 32 0.37 At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 32 0.48 At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 31 0.64 At4g25434.1 68417.m03661 MutT/nudix family protein similar to SP... 29 2.6 At5g51230.2 68418.m06353 embryonic flower 2 (EMF2) identical to ... 29 3.4 At5g51230.1 68418.m06352 embryonic flower 2 (EMF2) identical to ... 29 3.4 At1g65710.1 68414.m07458 expressed protein ; expression supporte... 29 4.5 At4g14830.1 68417.m02280 expressed protein 28 6.0 At3g45690.1 68416.m04938 proton-dependent oligopeptide transport... 28 6.0 >At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HSP17.4-CI) identical to 17.4 kDa class I heat shock protein SP:P19036 from [Arabidopsis thaliana] Length = 156 Score = 32.3 bits (70), Expect = 0.37 Identities = 15/34 (44%), Positives = 24/34 (70%) Frame = +3 Query: 408 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAP 509 S +F+RR+ LPE A E V++ + +GVL++T P Sbjct: 108 SGKFMRRFRLPENAKVEEVKASM-ENGVLSVTVP 140 >At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein (HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha crystallin family; identified in Scharf, K-D., et al, Cell Stress & Chaperones (2001) 6: 225-237. Length = 153 Score = 32.3 bits (70), Expect = 0.37 Identities = 15/34 (44%), Positives = 22/34 (64%) Frame = +3 Query: 408 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAP 509 S QF RR+ LPE + V++ + +GVLT+T P Sbjct: 105 SGQFTRRFRLPENVKMDQVKAAM-ENGVLTVTVP 137 >At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HSP18.1-CI) identical to 18.2 kDa class I heat shock protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana]; contains Pfam profile: PF00011 Hsp20/alpha crystallin family Length = 161 Score = 31.9 bits (69), Expect = 0.48 Identities = 15/34 (44%), Positives = 23/34 (67%) Frame = +3 Query: 408 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAP 509 S +F+RR+ LPE A E V++ + +GVLT+ P Sbjct: 111 SGKFMRRFRLPENAKMEEVKATM-ENGVLTVVVP 143 >At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein (HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853) (GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res. 17 (19), 7995 (1989)) Length = 157 Score = 31.5 bits (68), Expect = 0.64 Identities = 14/34 (41%), Positives = 23/34 (67%) Frame = +3 Query: 408 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAP 509 S +F RR+ LPE A E +++ + +GVL++T P Sbjct: 109 SGKFTRRFRLPENAKMEEIKASM-ENGVLSVTVP 141 >At4g25434.1 68417.m03661 MutT/nudix family protein similar to SP|P53370 Nucleoside diphosphate-linked moiety X motif 6 {Homo sapiens}; contains Pfam profile PF00293: NUDIX domain Length = 304 Score = 29.5 bits (63), Expect = 2.6 Identities = 14/36 (38%), Positives = 18/36 (50%) Frame = -1 Query: 659 EQIKMHHCKPPYLFSASWVPSLWSLISLRNGTGSVR 552 E + HH +P YL W+P S I L N + VR Sbjct: 80 EGFRYHHAEPTYLMLVYWIPEAESTIPL-NASHRVR 114 >At5g51230.2 68418.m06353 embryonic flower 2 (EMF2) identical to embryonic flower 2 [Arabidopsis thaliana] GI:14276050; supporting cDNA gi|14276049|dbj|AB053171.1| Length = 626 Score = 29.1 bits (62), Expect = 3.4 Identities = 12/26 (46%), Positives = 16/26 (61%) Frame = -2 Query: 562 VLCDGHLSLSLDGVGYLPGAVMVRTP 485 VL DGH+ + + L G +MVRTP Sbjct: 565 VLADGHIPWACEAFSRLHGPIMVRTP 590 >At5g51230.1 68418.m06352 embryonic flower 2 (EMF2) identical to embryonic flower 2 [Arabidopsis thaliana] GI:14276050; supporting cDNA gi|14276049|dbj|AB053171.1| Length = 631 Score = 29.1 bits (62), Expect = 3.4 Identities = 12/26 (46%), Positives = 16/26 (61%) Frame = -2 Query: 562 VLCDGHLSLSLDGVGYLPGAVMVRTP 485 VL DGH+ + + L G +MVRTP Sbjct: 570 VLADGHIPWACEAFSRLHGPIMVRTP 595 >At1g65710.1 68414.m07458 expressed protein ; expression supported by MPSS Length = 455 Score = 28.7 bits (61), Expect = 4.5 Identities = 15/29 (51%), Positives = 15/29 (51%) Frame = +3 Query: 513 RYPTPSRERERCPSHRTGPVSQGDQGPER 599 R TPSRERER S G V G G R Sbjct: 169 RRRTPSRERERSGSRERGNVGGGGGGSRR 197 >At4g14830.1 68417.m02280 expressed protein Length = 152 Score = 28.3 bits (60), Expect = 6.0 Identities = 13/30 (43%), Positives = 15/30 (50%) Frame = +3 Query: 420 VRRYALPEGAAPETVESRLSSDGVLTITAP 509 V R+ LPE PE V DG L +T P Sbjct: 109 VWRFRLPESTRPELVTVDCDGDGELIVTVP 138 >At3g45690.1 68416.m04938 proton-dependent oligopeptide transport (POT) family protein contains Pfam profile: PF00854 POT family Length = 516 Score = 28.3 bits (60), Expect = 6.0 Identities = 12/29 (41%), Positives = 19/29 (65%) Frame = -2 Query: 580 PCETGPVLCDGHLSLSLDGVGYLPGAVMV 494 PCETG +LC+ L L G+ Y+ A+++ Sbjct: 120 PCETGSILCESPSKLQL-GILYIALALVI 147 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,914,654 Number of Sequences: 28952 Number of extensions: 334945 Number of successful extensions: 934 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 905 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 934 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1721869952 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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