Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30461
(674 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g16640.1 68416.m02127 translationally controlled tumor family... 56 2e-08
At3g05540.1 68416.m00607 translationally controlled tumor family... 55 4e-08
At4g29060.1 68417.m04157 elongation factor Ts family protein sim... 33 0.23
At2g17050.1 68415.m01968 disease resistance protein (TIR-NBS-LRR... 31 0.70
At1g63910.1 68414.m07236 myb family transcription factor (MYB103... 28 6.6
At5g49030.1 68418.m06067 tRNA synthetase class I (I, L, M and V)... 27 8.7
At2g46060.2 68415.m05730 transmembrane protein-related contains ... 27 8.7
At2g46060.1 68415.m05729 transmembrane protein-related contains ... 27 8.7
At1g08710.1 68414.m00967 F-box family protein similar to ESTs gb... 27 8.7
>At3g16640.1 68416.m02127 translationally controlled tumor family
protein similar to translationally controlled tumor
protein GB:AAD10032 from [Hevea brasiliensis]
Length = 168
Score = 56.4 bits (130), Expect = 2e-08
Identities = 39/106 (36%), Positives = 51/106 (48%)
Frame = +1
Query: 259 VDIVLNHRLVETYAFGDKKSYTLYLKDYMKKLVAKLEEKAPDQVEVFKTNMNKVMKDILG 438
VDIV RL E + DKK + Y+K Y+K L KL E+ DQ VFK + K +L
Sbjct: 68 VDIVDTFRLQEQPTY-DKKGFIAYIKKYIKLLTPKLSEE--DQA-VFKKGIEGATKFLLP 123
Query: 439 RFKELQFFTGESMDCDGMFAMMELETLMVRKYQFMMFFKHGLEEEK 576
R + QFF GE M D + ++F HGL+E K
Sbjct: 124 RLSDFQFFVGEGMHDDSTLVFAYYKEGSTN--PTFLYFAHGLKEVK 167
Score = 56.0 bits (129), Expect = 2e-08
Identities = 28/66 (42%), Positives = 44/66 (66%), Gaps = 3/66 (4%)
Frame = +2
Query: 65 MKIYKDIITGDEMFSDTYKMKLVDE-VIYEVTGRLVTRAQGDIQIEGFNPSAEEA--DEG 235
M +Y+D++TGDE+ SD++ K ++ +++EV G+ VT D+ I G NPSAEE DEG
Sbjct: 1 MLVYQDLLTGDELLSDSFPYKEIENGILWEVEGKWVTVGAVDVNI-GANPSAEEGGEDEG 59
Query: 236 TDSAVE 253
D + +
Sbjct: 60 VDDSTQ 65
>At3g05540.1 68416.m00607 translationally controlled tumor family
protein similar to translationally controlled tumor
protein GB:AAD10032 from [Hevea brasiliensis]
Length = 156
Score = 55.2 bits (127), Expect = 4e-08
Identities = 28/74 (37%), Positives = 46/74 (62%)
Frame = +1
Query: 259 VDIVLNHRLVETYAFGDKKSYTLYLKDYMKKLVAKLEEKAPDQVEVFKTNMNKVMKDILG 438
VDI+ RL E +F DKK + +++K Y+K+L KL+ + E+FK ++ K ++
Sbjct: 55 VDIIDTFRLQEQPSF-DKKQFVMFMKRYIKQLSPKLDS---ENQELFKKHIESATKFLMS 110
Query: 439 RFKELQFFTGESMD 480
+ K+ QFF GESM+
Sbjct: 111 KLKDFQFFVGESME 124
Score = 36.7 bits (81), Expect = 0.014
Identities = 18/52 (34%), Positives = 34/52 (65%), Gaps = 1/52 (1%)
Frame = +2
Query: 65 MKIYKDIITGDEMFSDTYKMKLVDE-VIYEVTGRLVTRAQGDIQIEGFNPSA 217
M +Y+DI+TGDE+ SD++ K ++ +++EV G+ + +G + EG + A
Sbjct: 1 MLVYQDILTGDELLSDSFPYKEIENGMLWEVEGKNPSGEEGG-EDEGVDDQA 51
>At4g29060.1 68417.m04157 elongation factor Ts family protein
similar to SP|P35019 Elongation factor Ts (EF-Ts)
{Galdieria sulphuraria}; contains Pfam profiles PF00627:
UBA/TS-N domain, PF00889: Elongation factor TS, PF00575:
S1 RNA binding domain
Length = 953
Score = 32.7 bits (71), Expect = 0.23
Identities = 20/53 (37%), Positives = 31/53 (58%), Gaps = 2/53 (3%)
Frame = +2
Query: 92 GDEMFSDTYKMKLVDEVIYEVT--GRLVTRAQGDIQIEGFNPSAEEADEGTDS 244
G+ S K +++D V+ +T G +T +G+ EGF P+AEEAD+G S
Sbjct: 238 GEGFNSKFAKGQMLDGVVKNLTRSGAFITIGEGE---EGFLPTAEEADDGIGS 287
>At2g17050.1 68415.m01968 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1355
Score = 31.1 bits (67), Expect = 0.70
Identities = 17/49 (34%), Positives = 26/49 (53%), Gaps = 1/49 (2%)
Frame = +1
Query: 337 DYMKKLVAKLEEKAPDQVEVFKTNMNKVMKDILGRFKELQ-FFTGESMD 480
D M+ + KL P +EVFK+N N + ++ F ++ FF GE D
Sbjct: 226 DKMEVKLLKLNHPPPQIMEVFKSNYNALNENEKSMFLDIACFFRGEKAD 274
>At1g63910.1 68414.m07236 myb family transcription factor (MYB103)
contains Pfam profile: PF00249 myb-like DNA-binding
domain
Length = 370
Score = 27.9 bits (59), Expect = 6.6
Identities = 17/52 (32%), Positives = 22/52 (42%)
Frame = -2
Query: 175 SRHQPTGHFVNNFIDQFHFVGVREHLITSDNVLIDLHFDGLEAIKNNKNRKN 20
SRHQP+ V D E T+ + + +LHFDG N N N
Sbjct: 124 SRHQPSVTTVTLNADTTSIATTIEASTTTTSTIDNLHFDGFTDSPNQLNFTN 175
>At5g49030.1 68418.m06067 tRNA synthetase class I (I, L, M and V)
family protein similar to SP|P41972 Isoleucyl-tRNA
synthetase (EC 6.1.1.5) (Isoleucine--tRNA ligase)
(IleRS) {Staphylococcus aureus}; contains Pfam profile
PF00133: tRNA synthetases class I (I, L, M and V)
Length = 1093
Score = 27.5 bits (58), Expect = 8.7
Identities = 12/31 (38%), Positives = 20/31 (64%), Gaps = 3/31 (9%)
Frame = +1
Query: 409 MNKVMKDILGRFKELQFFTGE---SMDCDGM 492
+NK++KDI+ R+K LQ + + DC G+
Sbjct: 167 LNKILKDIINRYKLLQNYKVQYVPGWDCHGL 197
>At2g46060.2 68415.m05730 transmembrane protein-related contains
weak similarity to Swiss-Prot:Q9HCN3 transmembrane
protein 8 precursor (M83 protein) [Homo sapiens]
Length = 766
Score = 27.5 bits (58), Expect = 8.7
Identities = 19/67 (28%), Positives = 29/67 (43%)
Frame = -2
Query: 397 ILQLGQAPSLPILLLIFSYSL*GTMCRISCHRRRMFRLACGSGLCQLRLDGRVRALVSLF 218
+L L QA ++++ + G + +R CGS L + R RA+ F
Sbjct: 660 LLALTQATRASNIIIVLAIGSLGLLIGFLVEFVTKYRSYCGSAGFSLNMLDRPRAVKEWF 719
Query: 217 SRRIKTL 197
S IKTL
Sbjct: 720 SNLIKTL 726
>At2g46060.1 68415.m05729 transmembrane protein-related contains
weak similarity to Swiss-Prot:Q9HCN3 transmembrane
protein 8 precursor (M83 protein) [Homo sapiens]
Length = 807
Score = 27.5 bits (58), Expect = 8.7
Identities = 19/67 (28%), Positives = 29/67 (43%)
Frame = -2
Query: 397 ILQLGQAPSLPILLLIFSYSL*GTMCRISCHRRRMFRLACGSGLCQLRLDGRVRALVSLF 218
+L L QA ++++ + G + +R CGS L + R RA+ F
Sbjct: 660 LLALTQATRASNIIIVLAIGSLGLLIGFLVEFVTKYRSYCGSAGFSLNMLDRPRAVKEWF 719
Query: 217 SRRIKTL 197
S IKTL
Sbjct: 720 SNLIKTL 726
>At1g08710.1 68414.m00967 F-box family protein similar to ESTs
gb|T22270 and gb|T76886 ; similar to SKP1 interacting
partner 2 (SKIP2) TIGR_Ath1:At5g67250
Length = 274
Score = 27.5 bits (58), Expect = 8.7
Identities = 15/33 (45%), Positives = 18/33 (54%)
Frame = -2
Query: 349 FSYSL*GTMCRISCHRRRMFRLACGSGLCQLRL 251
FSY +C IS RR+FRL+C L L L
Sbjct: 24 FSYK---DLCCISISSRRLFRLSCDDSLWDLLL 53
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,800,989
Number of Sequences: 28952
Number of extensions: 273937
Number of successful extensions: 686
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 662
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 684
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1432596384
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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