BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30460 (647 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical... 58 5e-09 At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO) ... 58 7e-09 At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO) ... 48 7e-06 At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO) ... 35 0.041 At1g68185.1 68414.m07789 ubiquitin-related similar to ubiquitin-... 34 0.071 At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351 ... 34 0.094 At2g34960.1 68415.m04290 amino acid permease family protein simi... 28 6.1 >At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical to Ubiquitin-like protein SMT3 SP:P55852 from[Arabidopsis thaliana]; identical to cDNA SMT3 protein GI:1707371 Length = 100 Score = 58.0 bits (134), Expect = 5e-09 Identities = 28/51 (54%), Positives = 35/51 (68%), Gaps = 1/51 (1%) Frame = +1 Query: 106 DEKKGEN-EHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVV 255 D+K G+ HINLKV GQD V F+IK+ T L+KLMNAYCDR + M + Sbjct: 8 DKKPGDGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDMNSI 58 Score = 39.1 bits (87), Expect = 0.002 Identities = 16/34 (47%), Positives = 21/34 (61%) Frame = +3 Query: 258 FRFDGQPINENDTPTSLEMEEGDTIEVYQQQTGG 359 F FDG+ + TP L+ME+GD I+ QTGG Sbjct: 60 FLFDGRRLRAEQTPDELDMEDGDEIDAMLHQTGG 93 >At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO) similar to ubiquitin-like protein SMT3 SP:P55852 from [Arabidopsis thaliana]; identical to cDNA small ubiquitin-like modifier 2 (SUMO) GI:22652843; contains Pfam profile PF00240: Ubiquitin family Length = 103 Score = 57.6 bits (133), Expect = 7e-09 Identities = 26/50 (52%), Positives = 33/50 (66%) Frame = +1 Query: 106 DEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVV 255 D+K + HINLKV GQD V F+IK+ T L+KLMNAYCDR + + Sbjct: 8 DKKPDQGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDFNSI 57 Score = 40.3 bits (90), Expect = 0.001 Identities = 17/34 (50%), Positives = 21/34 (61%) Frame = +3 Query: 258 FRFDGQPINENDTPTSLEMEEGDTIEVYQQQTGG 359 F FDG+ + TP LEME+GD I+ QTGG Sbjct: 59 FLFDGRRLRAEQTPDELEMEDGDEIDAMLHQTGG 92 >At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO) similar to SP|O13351 Ubiquitin-like protein smt3/pmt3 {Schizosaccharomyces pombe}; identical to cDNA small ubiquitin-like modifier 3 (SUMO) GI:22652845 Length = 111 Score = 47.6 bits (108), Expect = 7e-06 Identities = 22/42 (52%), Positives = 26/42 (61%) Frame = +1 Query: 121 ENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSM 246 + H+ LKV QD V FK KK PL+KLM YCDR GL + Sbjct: 14 QEAHVILKVKSQDGDEVLFKNKKSAPLKKLMYVYCDRRGLKL 55 Score = 33.5 bits (73), Expect = 0.12 Identities = 14/35 (40%), Positives = 22/35 (62%) Frame = +3 Query: 258 FRFDGQPINENDTPTSLEMEEGDTIEVYQQQTGGV 362 F F+G I +TP L+ME+GD I+ + +GG+ Sbjct: 60 FIFNGARIGGLETPDELDMEDGDVIDACRAMSGGL 94 >At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO) similar to ubiquitin-like protein SMT3 SP:P55852 [Arabidopsis thaliana]; contains INTERPRO:IPR000626 ubiquitin domain; contains Pfam profile PF00240: Ubiquitin family; contains Pfam profile PF00240: Ubiquitin family; identical to cDNA small ubiquitin-like modifier 5 (SUMO) mRNA GI:22652847 Length = 108 Score = 35.1 bits (77), Expect = 0.041 Identities = 18/56 (32%), Positives = 27/56 (48%) Frame = +1 Query: 88 INLKMADEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVV 255 ++ K + + + LKV Q A +KI H L+KLM+AYC + L V Sbjct: 13 VSKKSRSPETSPHMKVTLKVKNQQGAEDLYKIGTHAHLKKLMSAYCTKRNLDYSSV 68 Score = 30.3 bits (65), Expect = 1.2 Identities = 14/35 (40%), Positives = 19/35 (54%) Frame = +3 Query: 255 RFRFDGQPINENDTPTSLEMEEGDTIEVYQQQTGG 359 RF ++G+ I TP L MEE D I + + GG Sbjct: 69 RFVYNGREIKARQTPAQLHMEEEDEICMVMELGGG 103 >At1g68185.1 68414.m07789 ubiquitin-related similar to ubiquitin-like protein smt3/pmt3 SP:O13351 from [Fission yeast] Length = 215 Score = 34.3 bits (75), Expect = 0.071 Identities = 15/32 (46%), Positives = 22/32 (68%) Frame = +3 Query: 258 FRFDGQPINENDTPTSLEMEEGDTIEVYQQQT 353 F FDG I+ + TP+ L ME+ D IEV+ ++T Sbjct: 184 FIFDGDKIDPSTTPSELGMEDHDMIEVHTKKT 215 >At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351 Ubiquitin-like protein smt3/pmt3 {Schizosaccharomyces pombe} Length = 114 Score = 33.9 bits (74), Expect = 0.094 Identities = 16/35 (45%), Positives = 19/35 (54%) Frame = +3 Query: 255 RFRFDGQPINENDTPTSLEMEEGDTIEVYQQQTGG 359 RF FDG I E TP LE ++GD I+ Q G Sbjct: 70 RFLFDGSRIREYHTPDELERKDGDEIDAMLCQQSG 104 >At2g34960.1 68415.m04290 amino acid permease family protein similar to cationic amino acid transporter 3 [Rattus norvegicus] GI:2116552; contains Pfam profile PF00324: Amino acid permease Length = 569 Score = 27.9 bits (59), Expect = 6.1 Identities = 15/37 (40%), Positives = 19/37 (51%) Frame = -1 Query: 299 WSVILIYWLPIKSESTTCIDRPALSQ*AFINFLRGVC 189 W V L+ WLP S +T +L AF+ F GVC Sbjct: 507 WGVPLVPWLPCLSIATNIFLMGSLGAMAFVRF--GVC 541 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,669,448 Number of Sequences: 28952 Number of extensions: 243931 Number of successful extensions: 481 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 473 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 481 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1344285648 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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