Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30456
(752 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 155 6e-39
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 152 5e-38
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 74 2e-14
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 53 4e-08
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 31 0.23
SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|c... 31 0.23
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 27 2.2
SPBC428.07 |meu6||meiotic chromosome segregation protein Meu6|Sc... 27 2.9
SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.0
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 5.0
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 26 6.6
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 8.8
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 25 8.8
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 155 bits (376), Expect = 6e-39
Identities = 73/103 (70%), Positives = 83/103 (80%)
Frame = +3
Query: 255 RNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAP 434
RNLDIERP+Y NLNRLI Q+VSSITASLRF+G+LNVDL EFQTNLVPYPRIHFPLVTYAP
Sbjct: 215 RNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAP 274
Query: 435 VISAEKAYHEQLSVAEITNACFEPATRW*NATPVMASTWLAVC 563
++SA KA+HE SV EITN CFEP + P A ++A C
Sbjct: 275 IVSAAKAFHESNSVQEITNQCFEPYNQMVKCDP-RAGRYMATC 316
Score = 138 bits (335), Expect = 6e-34
Identities = 59/81 (72%), Positives = 69/81 (85%)
Frame = +2
Query: 509 NQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQP 688
NQMVKCDPR G+YMA C+LYRGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI +P
Sbjct: 300 NQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRP 359
Query: 689 PTVVPGXDLAKVQRAVCMLSN 751
P + G ++AKV RAVCMLSN
Sbjct: 360 PQHIEGSEIAKVDRAVCMLSN 380
Score = 121 bits (291), Expect = 1e-28
Identities = 53/83 (63%), Positives = 66/83 (79%)
Frame = +1
Query: 7 LQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTT 186
LQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTT
Sbjct: 132 LQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTT 191
Query: 187 HTTLEHSDCAFMVDNEAIYDICR 255
H TL+ +DC FMVDNE+ YDICR
Sbjct: 192 HATLDLADCTFMVDNESCYDICR 214
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 152 bits (369), Expect = 5e-38
Identities = 72/103 (69%), Positives = 81/103 (78%)
Frame = +3
Query: 255 RNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAP 434
RNLDIERPTY NLNRLI Q+VSSITASLRF G+LNVDL EFQTNLVPYPRIHFPLVTY+P
Sbjct: 219 RNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSP 278
Query: 435 VISAEKAYHEQLSVAEITNACFEPATRW*NATPVMASTWLAVC 563
++SA KA+HE SV EITN CFEP + P ++A C
Sbjct: 279 IVSAAKAFHESNSVQEITNQCFEPYNQMVKCDP-RTGRYMATC 320
Score = 143 bits (346), Expect = 3e-35
Identities = 60/81 (74%), Positives = 71/81 (87%)
Frame = +2
Query: 509 NQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQP 688
NQMVKCDPR G+YMA C+LYRGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+P
Sbjct: 304 NQMVKCDPRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEP 363
Query: 689 PTVVPGXDLAKVQRAVCMLSN 751
P VPG +AKV RAVCMLSN
Sbjct: 364 PQHVPGSGIAKVNRAVCMLSN 384
Score = 126 bits (304), Expect = 3e-30
Identities = 55/83 (66%), Positives = 67/83 (80%)
Frame = +1
Query: 7 LQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTT 186
LQGFL+FH LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTT
Sbjct: 136 LQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTT 195
Query: 187 HTTLEHSDCAFMVDNEAIYDICR 255
H TL++SDC FMVDNEA YDICR
Sbjct: 196 HATLDNSDCTFMVDNEACYDICR 218
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 74.1 bits (174), Expect = 2e-14
Identities = 31/81 (38%), Positives = 52/81 (64%)
Frame = +3
Query: 261 LDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVI 440
L I+ P+Y +LN L+ +++ +T S RF G LN DL + N+VP+PR+HF +V +AP+
Sbjct: 215 LKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLA 274
Query: 441 SAEKAYHEQLSVAEITNACFE 503
+ + + +SV E+T F+
Sbjct: 275 AIGSSSFQAVSVPELTQQMFD 295
Score = 64.9 bits (151), Expect = 1e-11
Identities = 31/82 (37%), Positives = 46/82 (56%)
Frame = +1
Query: 4 ALQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILT 183
ALQGF + H LL+ ++ +Y + F++ PAP+ S VVEPYN+ L+
Sbjct: 129 ALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLS 188
Query: 184 THTTLEHSDCAFMVDNEAIYDI 249
H +E+SD F +DNEA+ I
Sbjct: 189 MHQLVENSDETFCIDNEALSSI 210
Score = 60.9 bits (141), Expect = 2e-10
Identities = 26/68 (38%), Positives = 39/68 (57%)
Frame = +2
Query: 488 KRMLRARNQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFK 667
++M A N MV DPRHG+Y+ L+RG V K+V+ I +++TK + FV+W P
Sbjct: 291 QQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVL 350
Query: 668 VGINYQPP 691
+ PP
Sbjct: 351 KAVCSVPP 358
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 53.2 bits (122), Expect = 4e-08
Identities = 22/58 (37%), Positives = 35/58 (60%)
Frame = +3
Query: 261 LDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAP 434
L + PT+ N+L+ ++S+ T +LR+ G +N DL +L+P PR HF L +Y P
Sbjct: 218 LHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTP 275
Score = 48.8 bits (111), Expect = 8e-07
Identities = 27/83 (32%), Positives = 43/83 (51%), Gaps = 1/83 (1%)
Frame = +1
Query: 4 ALQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSIL 180
+L+GF + H L+ERL+ Y KK ++++P Q VS VV+PYNS+L
Sbjct: 131 SLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLL 190
Query: 181 TTHTTLEHSDCAFMVDNEAIYDI 249
++D ++DN A+ I
Sbjct: 191 ALKRLTLNADSVVVLDNAALAHI 213
Score = 42.7 bits (96), Expect = 5e-05
Identities = 18/71 (25%), Positives = 40/71 (56%), Gaps = 1/71 (1%)
Frame = +2
Query: 488 KRMLRARNQMVKCDP-RHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGF 664
+R+L +NQMV +P + +++ + +G+ P DV+ ++ I+ +R F+ W P
Sbjct: 297 RRLLLPKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASI 356
Query: 665 KVGINYQPPTV 697
+V ++ + P +
Sbjct: 357 QVALSKKSPYI 367
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 30.7 bits (66), Expect = 0.23
Identities = 16/45 (35%), Positives = 21/45 (46%)
Frame = +3
Query: 570 VVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 704
VV P + RP++P P LS V PV+ V + PP P
Sbjct: 552 VVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAP 596
>SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|chr
1|||Manual
Length = 474
Score = 30.7 bits (66), Expect = 0.23
Identities = 17/47 (36%), Positives = 28/47 (59%)
Frame = +1
Query: 394 LTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPQPDGEMRPP 534
LTP + S +S + +S +R ++N+ PS + +H+SS Q RPP
Sbjct: 130 LTPKNPSLFSSSNAAS--QRGSLNTAPSNFAYSHSSSLQTSASSRPP 174
>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 3227
Score = 27.5 bits (58), Expect = 2.2
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = -2
Query: 610 SDGRVHILGYDVTTVQHTASHVLAMTGVAFHHLVAGSKHAF 488
S G +LGY ++ A++V+A + V HL+ G AF
Sbjct: 407 SAGLTSLLGYHLSVKTPQATYVVARSIVMLDHLIDGYSMAF 447
>SPBC428.07 |meu6||meiotic chromosome segregation protein
Meu6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 651
Score = 27.1 bits (57), Expect = 2.9
Identities = 20/64 (31%), Positives = 30/64 (46%), Gaps = 2/64 (3%)
Frame = +3
Query: 351 ALNVDLTEFQTNLVPYPRIHFPLVT--YAPVISAEKAYHEQLSVAEITNACFEPATRW*N 524
A +V++ E +TN +P HFP T AP S EK + T + +T+ N
Sbjct: 368 AQSVEVPENETNQIPTTEEHFPATTEEVAPA-SEEKPATGPAEESTSTQNVEQASTQNDN 426
Query: 525 ATPV 536
TP+
Sbjct: 427 GTPI 430
>SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 751
Score = 26.2 bits (55), Expect = 5.0
Identities = 16/48 (33%), Positives = 27/48 (56%)
Frame = +1
Query: 367 SPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPQ 510
SPS LT L S++ S + + + R+ T +S SP+ Q+ S+P+
Sbjct: 619 SPSKMLT-TLRNNSSTFPSLRKNAMIARKSTADSLSSPKRQSVPSTPK 665
>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 628
Score = 26.2 bits (55), Expect = 5.0
Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%)
Frame = +3
Query: 606 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 692
+LP KPS+ +++S V+P S STTS+P
Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 25.8 bits (54), Expect = 6.6
Identities = 10/20 (50%), Positives = 11/20 (55%)
Frame = -1
Query: 587 WVRRHHGTAYSKPCTCHDGG 528
W R H Y+KPC DGG
Sbjct: 16 WRRDHPFGFYAKPCKSSDGG 35
>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 665
Score = 25.4 bits (53), Expect = 8.8
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%)
Frame = +3
Query: 603 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 698
P PS+P+++SN ST G+Q V V + W
Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 25.4 bits (53), Expect = 8.8
Identities = 17/56 (30%), Positives = 23/56 (41%)
Frame = +3
Query: 525 ATPVMASTWLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHP 692
+TPV ++ CT TS P T + S P +N +T S TS P
Sbjct: 437 STPVTSTPLATTNCTTSTSVPY--TSTPVTSTPLTTTNCTTSTSIPYTSTPVTSTP 490
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,223,667
Number of Sequences: 5004
Number of extensions: 69198
Number of successful extensions: 277
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 255
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 276
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 359287726
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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