Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30446
(692 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g02210.1 68417.m00298 expressed protein 32 0.31
At1g19230.1 68414.m02393 respiratory burst oxidase protein E (Rb... 30 1.7
At4g39790.1 68417.m05634 expressed protein ; expression support... 29 2.2
At1g78270.1 68414.m09121 UDP-glucose glucosyltransferase, putati... 29 2.9
At4g10270.1 68417.m01688 wound-responsive family protein similar... 29 3.9
At4g35560.1 68417.m05053 expressed protein 28 5.1
At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase famil... 28 5.1
At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase famil... 28 5.1
At1g64610.2 68414.m07324 WD-40 repeat family protein contains Pf... 28 5.1
At1g64610.1 68414.m07323 WD-40 repeat family protein contains Pf... 28 5.1
At4g31390.1 68417.m04452 ABC1 family protein contains Pfam domai... 28 6.8
At1g59453.1 68414.m06679 transcription factor-related weak simil... 28 6.8
At5g03140.1 68418.m00262 lectin protein kinase family protein co... 27 8.9
At5g02990.1 68418.m00243 kelch repeat-containing F-box family pr... 27 8.9
At2g35110.1 68415.m04307 HEM protein-related weak similarity to ... 27 8.9
>At4g02210.1 68417.m00298 expressed protein
Length = 439
Score = 32.3 bits (70), Expect = 0.31
Identities = 18/54 (33%), Positives = 28/54 (51%)
Frame = +1
Query: 496 QLGVFRSIEERVGSAYENVKGKVASRSNSTQSFDEALRDASGATSPPIPEHKPL 657
Q +SI G A++N + V + +N Q + +A RDA + PIP +K L
Sbjct: 247 QFNAIKSILRSDGFAWDNERQMVTADNNVWQDYIKAHRDARQFMTRPIPYYKDL 300
>At1g19230.1 68414.m02393 respiratory burst oxidase protein E
(RbohE) / NADPH oxidase nearly identical to respiratory
burst oxidase protein E GI:3242787 [gi:3242787] from
[Arabidopsis thaliana]
Length = 926
Score = 29.9 bits (64), Expect = 1.7
Identities = 14/25 (56%), Positives = 17/25 (68%)
Frame = +1
Query: 67 HSFNTVRVFHCKI*AVFKQLKKQCQ 141
H +TV VF+C I V K+LKKQ Q
Sbjct: 885 HPNSTVGVFYCGIQTVAKELKKQAQ 909
>At4g39790.1 68417.m05634 expressed protein ; expression supported
by MPSS
Length = 631
Score = 29.5 bits (63), Expect = 2.2
Identities = 17/56 (30%), Positives = 26/56 (46%)
Frame = +1
Query: 355 RQSGFEKRQRKPSIPKN*LCDKNDRREDVFDHRRHHGRRLQQARSDAQLGVFRSIE 522
+ S F+ +QRK S C+ ND RED + H + + D + FR+ E
Sbjct: 203 QDSSFKTKQRKQS------CEDNDEREDPSEFITHRAKDFVSSMKDIEHKFFRASE 252
>At1g78270.1 68414.m09121 UDP-glucose glucosyltransferase, putative
similar to UDP-glucose glucosyltransferase GI:3928543
from [Arabidopsis thaliana]
Length = 489
Score = 29.1 bits (62), Expect = 2.9
Identities = 19/95 (20%), Positives = 44/95 (46%)
Frame = +2
Query: 287 KIRQSSDLKRKLGITVWKEITEDVNQGLKNVKESQVYQKTDSVIKTTAEKTSSIIGGITA 466
+I ++S++ RKLG+ +W+E TE ++ +++ +Y S+ T+E+ G+
Sbjct: 265 EIDKNSEI-RKLGLNLWEEETESLDWLDTKAEKAVIYVNFGSLTVLTSEQILEFAWGLAR 323
Query: 467 GVSSKLGQMRNSESSAPSKNASARLMKTSRVKWLL 571
L +R+ A + ++ + +L
Sbjct: 324 SGKEFLWVVRSGMVDGDDSILPAEFLSETKNRGML 358
>At4g10270.1 68417.m01688 wound-responsive family protein similar to
wound induced protein (GI:19320) [Lycopersicon
esculentum]
Length = 90
Score = 28.7 bits (61), Expect = 3.9
Identities = 18/46 (39%), Positives = 22/46 (47%)
Frame = +2
Query: 308 LKRKLGITVWKEITEDVNQGLKNVKESQVYQKTDSVIKTTAEKTSS 445
LK +LG+ W I VNQ L+N S K S +A TSS
Sbjct: 20 LKDQLGLCRWNYILRSVNQHLRNNVRSVSQGKRFSSSSVSAAVTSS 65
>At4g35560.1 68417.m05053 expressed protein
Length = 917
Score = 28.3 bits (60), Expect = 5.1
Identities = 20/76 (26%), Positives = 36/76 (47%), Gaps = 5/76 (6%)
Frame = +1
Query: 415 DKNDRREDVFDHRRHHGRRLQQARSDAQLGVFRSI-EERVGSAYENVKGKV----ASRSN 579
D+ + D D HH + QQ + Q G+ + ++++ + + N KGK+ A
Sbjct: 773 DEEELDIDDIDIDDHHPNQQQQEKPKEQ-GILSGLSKQKMANRFSNFKGKLKQMAAKNEK 831
Query: 580 STQSFDEALRDASGAT 627
S + DE + +GAT
Sbjct: 832 SVVTNDEKHEEKNGAT 847
>At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS8) similar to LACS 4 [SP|O35547] from Rattus
norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
contains Pfam HMM hit: AMP-binding enzymes PF00501
Length = 720
Score = 28.3 bits (60), Expect = 5.1
Identities = 24/69 (34%), Positives = 33/69 (47%), Gaps = 3/69 (4%)
Frame = +2
Query: 356 VNQGLKNVKESQVYQKTDSVIKTTAEKTSSIIGGITAGVSS---KLGQMRNSESSAPSKN 526
+ LK VK +Y + D V +++ S +G IT S KLGQ + PSKN
Sbjct: 218 IQSSLKTVKNI-IYIEEDGVDVASSDVNS--MGDITVSSISEVEKLGQKNAVQPILPSKN 274
Query: 527 ASARLMKTS 553
A +M TS
Sbjct: 275 GVAVIMFTS 283
>At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS8) similar to LACS 4 [SP|O35547] from Rattus
norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
contains Pfam HMM hit: AMP-binding enzymes PF00501
Length = 720
Score = 28.3 bits (60), Expect = 5.1
Identities = 24/69 (34%), Positives = 33/69 (47%), Gaps = 3/69 (4%)
Frame = +2
Query: 356 VNQGLKNVKESQVYQKTDSVIKTTAEKTSSIIGGITAGVSS---KLGQMRNSESSAPSKN 526
+ LK VK +Y + D V +++ S +G IT S KLGQ + PSKN
Sbjct: 218 IQSSLKTVKNI-IYIEEDGVDVASSDVNS--MGDITVSSISEVEKLGQKNAVQPILPSKN 274
Query: 527 ASARLMKTS 553
A +M TS
Sbjct: 275 GVAVIMFTS 283
>At1g64610.2 68414.m07324 WD-40 repeat family protein contains Pfam
PF00400: WD domain, G-beta repeat; similar to WD-repeat
protein 5 (WD repeat protein BIG-3) (SP: Q9UGP9) [Homo
sapiens]
Length = 647
Score = 28.3 bits (60), Expect = 5.1
Identities = 24/98 (24%), Positives = 42/98 (42%)
Frame = +2
Query: 275 VLXSKIRQSSDLKRKLGITVWKEITEDVNQGLKNVKESQVYQKTDSVIKTTAEKTSSIIG 454
V+ + SS +K ++ + V E T + +V S +T + TS++ G
Sbjct: 73 VVSEVVNVSSGVK-EIDLLVRNESTTSSGFDVSSVSSSDTDDQTLLARNDASSSTSTMPG 131
Query: 455 GITAGVSSKLGQMRNSESSAPSKNASARLMKTSRVKWL 568
G++ SSK G + +S S+ L+K WL
Sbjct: 132 GLSESSSSKSGSFGDFHNSPFSQRDD--LLKKGAKSWL 167
>At1g64610.1 68414.m07323 WD-40 repeat family protein contains Pfam
PF00400: WD domain, G-beta repeat; similar to WD-repeat
protein 5 (WD repeat protein BIG-3) (SP: Q9UGP9) [Homo
sapiens]
Length = 647
Score = 28.3 bits (60), Expect = 5.1
Identities = 24/98 (24%), Positives = 42/98 (42%)
Frame = +2
Query: 275 VLXSKIRQSSDLKRKLGITVWKEITEDVNQGLKNVKESQVYQKTDSVIKTTAEKTSSIIG 454
V+ + SS +K ++ + V E T + +V S +T + TS++ G
Sbjct: 73 VVSEVVNVSSGVK-EIDLLVRNESTTSSGFDVSSVSSSDTDDQTLLARNDASSSTSTMPG 131
Query: 455 GITAGVSSKLGQMRNSESSAPSKNASARLMKTSRVKWL 568
G++ SSK G + +S S+ L+K WL
Sbjct: 132 GLSESSSSKSGSFGDFHNSPFSQRDD--LLKKGAKSWL 167
>At4g31390.1 68417.m04452 ABC1 family protein contains Pfam domain,
PF03109: ABC1 family
Length = 682
Score = 27.9 bits (59), Expect = 6.8
Identities = 13/44 (29%), Positives = 22/44 (50%)
Frame = +1
Query: 508 FRSIEERVGSAYENVKGKVASRSNSTQSFDEALRDASGATSPPI 639
F IEE +G EN+ K++S++ + S + R AT +
Sbjct: 219 FNIIEEELGQPLENIFSKISSQTIAAASLGQVYRATLRATGEDV 262
>At1g59453.1 68414.m06679 transcription factor-related weak similarity
to TFIIIC Box B-binding subunit [Homo sapiens] GI:442362
Length = 1729
Score = 27.9 bits (59), Expect = 6.8
Identities = 18/57 (31%), Positives = 28/57 (49%)
Frame = +2
Query: 338 KEITEDVNQGLKNVKESQVYQKTDSVIKTTAEKTSSIIGGITAGVSSKLGQMRNSES 508
K ++D N+ ++N S +K S++KT E SII ++S NSES
Sbjct: 907 KSNSKDKNKAVENSPSSSKKRKRASLVKTKGEGVKSIIVDGQKVLNSDAIDASNSES 963
>At5g03140.1 68418.m00262 lectin protein kinase family protein
contains Pfam domains, PF00138: Legume lectins alpha
domain, PF00139: Legume lectins beta domain and PF00069:
Protein kinase domain
Length = 711
Score = 27.5 bits (58), Expect = 8.9
Identities = 17/47 (36%), Positives = 25/47 (53%)
Frame = -2
Query: 250 STRASSRLHSARNWSACSGVRPANSSGVCMSPATPVPDIASSAV*TP 110
ST+ S+ +HS WS S ++ S M P +P P SS++ TP
Sbjct: 244 STQGSTEIHSVDWWSFSSSFEESSESPPPM-PNSPPPSSPSSSI-TP 288
>At5g02990.1 68418.m00243 kelch repeat-containing F-box family
protein contains Pfam profiles PF01344: Kelch motif,
PF00646: F-box domain
Length = 548
Score = 27.5 bits (58), Expect = 8.9
Identities = 12/41 (29%), Positives = 20/41 (48%)
Frame = +1
Query: 409 LCDKNDRREDVFDHRRHHGRRLQQARSDAQLGVFRSIEERV 531
+C + +R VFD R H RRL R V +++++
Sbjct: 328 VCGTSSKRVFVFDSRSHQWRRLHDMRLPRVSAVVNIVDKKI 368
>At2g35110.1 68415.m04307 HEM protein-related weak similarity to
Membrane-associated protein Hem (Dhem-2)
(Swiss-Prot:P55162) [Drosophila melanogaster]; weak
similarity to Nck-associated protein 1 (NAP 1)
(p125Nap1) (Membrane-associated protein HEM-2)
(Swiss-Prot:P55161) [Rattus norvegicus]
Length = 1339
Score = 27.5 bits (58), Expect = 8.9
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = -3
Query: 474 ETPAVMPPMIEDVFSAVVFITESVFWY 394
+ P+++ P I+ VFSA+ V WY
Sbjct: 417 DQPSLLAPNIQMVFSALALAQSEVLWY 443
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,720,295
Number of Sequences: 28952
Number of extensions: 239071
Number of successful extensions: 845
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 822
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 845
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1477286152
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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