Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30428
(385 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g63250.2 68416.m07106 homocysteine S-methyltransferase 2 (HMT... 68 2e-12
At3g63250.1 68416.m07107 homocysteine S-methyltransferase 2 (HMT... 68 2e-12
At3g25900.2 68416.m03227 homocysteine S-methyltransferase 1 (HMT... 67 3e-12
At3g25900.1 68416.m03228 homocysteine S-methyltransferase 1 (HMT... 67 3e-12
At3g22740.1 68416.m02868 homocysteine S-methyltransferase 3 (HMT... 59 1e-09
At5g15300.1 68418.m01792 pentatricopeptide (PPR) repeat-containi... 29 0.81
At5g16350.1 68418.m01911 expressed protein 27 5.7
At4g26750.1 68417.m03854 hydroxyproline-rich glycoprotein family... 26 7.5
At3g57430.1 68416.m06394 pentatricopeptide (PPR) repeat-containi... 26 7.5
>At3g63250.2 68416.m07106 homocysteine S-methyltransferase 2 (HMT-2)
99.7% identical to homocysteine S-methyltransferase
AtHMT-2 (GI:6685163) [Arabidopsis thaliana]
Length = 293
Score = 67.7 bits (158), Expect = 2e-12
Identities = 37/91 (40%), Positives = 59/91 (64%), Gaps = 6/91 (6%)
Frame = +2
Query: 2 HLDFLRAGADLIITNTYQASVEGFVEHLGVTKEQGYELIARAVQLAKQARTLYLEEYRDY 181
HLD+L AGAD+I + +YQA+++GF E G ++E+ L+ ++V++A +AR Y ++
Sbjct: 18 HLDYLEAGADIISSASYQATIQGF-EAKGFSREESESLLKKSVEIATEARNSYYDKCGTS 76
Query: 182 VQNDD-----IPLIV-GSVGPYGAHLHDGSD 256
DD P++V SVG YGA+L DGS+
Sbjct: 77 SSMDDKILKKRPILVAASVGSYGAYLADGSE 107
Score = 50.0 bits (114), Expect = 5e-07
Identities = 19/39 (48%), Positives = 29/39 (74%)
Frame = +1
Query: 256 YDGSYADTTSIETMREWHRPRIQALVEAGVDLLALETIP 372
Y G Y D+ ++E ++++HR R+Q L E+G DL+A ETIP
Sbjct: 108 YSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETIP 146
>At3g63250.1 68416.m07107 homocysteine S-methyltransferase 2 (HMT-2)
99.7% identical to homocysteine S-methyltransferase
AtHMT-2 (GI:6685163) [Arabidopsis thaliana]
Length = 333
Score = 67.7 bits (158), Expect = 2e-12
Identities = 37/91 (40%), Positives = 59/91 (64%), Gaps = 6/91 (6%)
Frame = +2
Query: 2 HLDFLRAGADLIITNTYQASVEGFVEHLGVTKEQGYELIARAVQLAKQARTLYLEEYRDY 181
HLD+L AGAD+I + +YQA+++GF E G ++E+ L+ ++V++A +AR Y ++
Sbjct: 58 HLDYLEAGADIISSASYQATIQGF-EAKGFSREESESLLKKSVEIATEARNSYYDKCGTS 116
Query: 182 VQNDD-----IPLIV-GSVGPYGAHLHDGSD 256
DD P++V SVG YGA+L DGS+
Sbjct: 117 SSMDDKILKKRPILVAASVGSYGAYLADGSE 147
Score = 50.0 bits (114), Expect = 5e-07
Identities = 19/39 (48%), Positives = 29/39 (74%)
Frame = +1
Query: 256 YDGSYADTTSIETMREWHRPRIQALVEAGVDLLALETIP 372
Y G Y D+ ++E ++++HR R+Q L E+G DL+A ETIP
Sbjct: 148 YSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETIP 186
>At3g25900.2 68416.m03227 homocysteine S-methyltransferase 1 (HMT-1)
identical to GB:AAF23821 from [Arabidopsis thaliana]
Length = 268
Score = 67.3 bits (157), Expect = 3e-12
Identities = 31/86 (36%), Positives = 58/86 (67%), Gaps = 1/86 (1%)
Frame = +2
Query: 2 HLDFLRAGADLIITNTYQASVEGFVEHLGVTKEQGYELIARAVQLAKQARTLYLEEYRDY 181
H+++L AGAD+++T++YQA++ GF+ G++ E+ L+ ++V+LA +AR + E+
Sbjct: 59 HMEYLEAGADIVVTSSYQATIPGFLSR-GLSIEESESLLQKSVELAVEARDRFWEKVSKV 117
Query: 182 VQND-DIPLIVGSVGPYGAHLHDGSD 256
+ + L+ S+G YGA+L DGS+
Sbjct: 118 SGHSYNRALVAASIGSYGAYLADGSE 143
Score = 51.6 bits (118), Expect = 2e-07
Identities = 21/39 (53%), Positives = 29/39 (74%)
Frame = +1
Query: 256 YDGSYADTTSIETMREWHRPRIQALVEAGVDLLALETIP 372
Y G Y + S++ ++++HR R+Q LVEAG DLLA ETIP
Sbjct: 144 YSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFETIP 182
>At3g25900.1 68416.m03228 homocysteine S-methyltransferase 1 (HMT-1)
identical to GB:AAF23821 from [Arabidopsis thaliana]
Length = 326
Score = 67.3 bits (157), Expect = 3e-12
Identities = 31/86 (36%), Positives = 58/86 (67%), Gaps = 1/86 (1%)
Frame = +2
Query: 2 HLDFLRAGADLIITNTYQASVEGFVEHLGVTKEQGYELIARAVQLAKQARTLYLEEYRDY 181
H+++L AGAD+++T++YQA++ GF+ G++ E+ L+ ++V+LA +AR + E+
Sbjct: 59 HMEYLEAGADIVVTSSYQATIPGFLSR-GLSIEESESLLQKSVELAVEARDRFWEKVSKV 117
Query: 182 VQND-DIPLIVGSVGPYGAHLHDGSD 256
+ + L+ S+G YGA+L DGS+
Sbjct: 118 SGHSYNRALVAASIGSYGAYLADGSE 143
Score = 51.6 bits (118), Expect = 2e-07
Identities = 21/39 (53%), Positives = 29/39 (74%)
Frame = +1
Query: 256 YDGSYADTTSIETMREWHRPRIQALVEAGVDLLALETIP 372
Y G Y + S++ ++++HR R+Q LVEAG DLLA ETIP
Sbjct: 144 YSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFETIP 182
>At3g22740.1 68416.m02868 homocysteine S-methyltransferase 3 (HMT-3)
identical to homocysteine S-methyltransferase HMT-3
[Arabidopsis thaliana] GI:9966515; similar to
homocysteine S-methyltransferase AtHMT-2 (GI:6685163)
[Arabidopsis thaliana]; similar to selenocysteine
methyltransferase GB:P56707 from [Astragalus bisulcatus]
Length = 347
Score = 58.8 bits (136), Expect = 1e-09
Identities = 34/94 (36%), Positives = 55/94 (58%), Gaps = 9/94 (9%)
Frame = +2
Query: 2 HLDFLRAGADLIITNTYQASVEGFVEHLGVTKEQGYELIARAVQLAKQARTLYLEE---- 169
HLD+L +GA++IIT +YQA+++GFV G++ + L+ R+V++ +AR ++
Sbjct: 62 HLDYLESGANIIITASYQATIQGFVAK-GLSVGEAENLLRRSVEITYEAREIFYNRCTKG 120
Query: 170 -----YRDYVQNDDIPLIVGSVGPYGAHLHDGSD 256
Y I L+ SVG YGA+L DGS+
Sbjct: 121 SWDFAYAGKASRRPI-LVAASVGSYGAYLADGSE 153
Score = 50.4 bits (115), Expect = 4e-07
Identities = 20/39 (51%), Positives = 29/39 (74%)
Frame = +1
Query: 256 YDGSYADTTSIETMREWHRPRIQALVEAGVDLLALETIP 372
Y G Y D+ S ET++++HR R+Q L ++G DL+A ETIP
Sbjct: 154 YSGIYGDSVSKETLKDFHRRRVQILAKSGADLIAFETIP 192
>At5g15300.1 68418.m01792 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 548
Score = 29.5 bits (63), Expect = 0.81
Identities = 14/57 (24%), Positives = 28/57 (49%)
Frame = -1
Query: 289 LLTWCLHNCHRIGTVVKMSSIWSDRTHYEWNVIILNVVTVFFQIEGAGLFSELNCAG 119
++T CL C + + ++ ++++ WN +I V + E G+F E+ AG
Sbjct: 215 MITGCL-KCKEMDSARELFDRFTEKDVVTWNAMISGYVNCGYPKEALGIFKEMRDAG 270
>At5g16350.1 68418.m01911 expressed protein
Length = 488
Score = 26.6 bits (56), Expect = 5.7
Identities = 15/49 (30%), Positives = 26/49 (53%)
Frame = -1
Query: 247 VVKMSSIWSDRTHYEWNVIILNVVTVFFQIEGAGLFSELNCAGY*FITL 101
+ +++ I DR+ W++ ILNV T + + G F+ G FI+L
Sbjct: 98 ISRLTLIPLDRSRPLWDIHILNVKTSYAEAVGVIRFNHALADGMSFISL 146
>At4g26750.1 68417.m03854 hydroxyproline-rich glycoprotein family
protein
Length = 421
Score = 26.2 bits (55), Expect = 7.5
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +2
Query: 188 NDDIPLIVGSVGPYGAHLHDGSDT 259
+DD L + S GP G++ H SDT
Sbjct: 159 DDDTDLSIPSSGPSGSYDHSASDT 182
>At3g57430.1 68416.m06394 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 890
Score = 26.2 bits (55), Expect = 7.5
Identities = 12/44 (27%), Positives = 19/44 (43%)
Frame = -1
Query: 319 FGADAIPA*SLLTWCLHNCHRIGTVVKMSSIWSDRTHYEWNVII 188
+G D++ + L C G V K+ S+R WN +I
Sbjct: 128 YGVDSVTVANTLVNLYRKCGDFGAVYKVFDRISERNQVSWNSLI 171
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,436,383
Number of Sequences: 28952
Number of extensions: 171676
Number of successful extensions: 401
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 383
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 396
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 537681456
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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