Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30417
(616 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g48760.1 68418.m06034 60S ribosomal protein L13A (RPL13aD) 114 4e-26
At3g24830.1 68416.m03115 60S ribosomal protein L13A (RPL13aB) si... 113 1e-25
At3g07110.1 68416.m00847 60S ribosomal protein L13A (RPL13aA) si... 113 1e-25
At4g13170.1 68417.m02049 60S ribosomal protein L13A (RPL13aC) ri... 112 2e-25
At3g10270.1 68416.m01231 DNA topoisomerase, ATP-hydrolyzing, put... 28 4.3
At5g11250.1 68418.m01314 disease resistance protein (TIR-NBS-LRR... 27 7.5
At5g03330.2 68418.m00285 OTU-like cysteine protease family prote... 27 7.5
At5g03330.1 68418.m00284 OTU-like cysteine protease family prote... 27 7.5
At1g49260.1 68414.m05523 hypothetical protein 27 7.5
At4g27590.1 68417.m03963 copper-binding protein-related low simi... 27 9.9
>At5g48760.1 68418.m06034 60S ribosomal protein L13A (RPL13aD)
Length = 206
Score = 114 bits (275), Expect = 4e-26
Identities = 50/84 (59%), Positives = 62/84 (73%)
Frame = +3
Query: 3 GFSNKAIVIDGRGHLLGRLAAVIAKVLLEGNKVVVVRCEQINISGNFFRNKLKLMSFLRK 182
G +K +V+D R H+LGRLA++ AK LL G KVV+VRCE+I +SG R K+K M FLRK
Sbjct: 6 GICSKRVVVDARHHMLGRLASITAKELLNGQKVVIVRCEEICLSGGLVRQKMKYMRFLRK 65
Query: 183 RCNVNPARGPFHFRAPSKILWKTV 254
R N P+ GP HFRAPSKI W+TV
Sbjct: 66 RMNTKPSHGPIHFRAPSKIFWRTV 89
Score = 78.6 bits (185), Expect = 3e-15
Identities = 37/90 (41%), Positives = 49/90 (54%)
Frame = +2
Query: 254 RGMIPHKTERGKNALRXLRTYDGCPPPFDNXXXXXXXXXXXXFCLKPGRNYCHVGRLSHE 433
RGMIPHKT+RG AL L+ Y+G P P+D L+ G YC +GRLS E
Sbjct: 90 RGMIPHKTKRGAAALARLKVYEGVPTPYDKIKRMVIPDALKVLRLQAGHKYCLLGRLSSE 149
Query: 434 IGWKYRDVVRKLEDKRXGXAVXRVAMKRNL 523
+GW + D +++LE KR A K+ L
Sbjct: 150 VGWNHYDTIKELETKRKERAHVVYERKKQL 179
>At3g24830.1 68416.m03115 60S ribosomal protein L13A (RPL13aB)
similar to 60S RIBOSOMAL PROTEIN L13A GB:P35427 from
[Rattus norvegicus]
Length = 206
Score = 113 bits (271), Expect = 1e-25
Identities = 50/84 (59%), Positives = 61/84 (72%)
Frame = +3
Query: 3 GFSNKAIVIDGRGHLLGRLAAVIAKVLLEGNKVVVVRCEQINISGNFFRNKLKLMSFLRK 182
G +K +V+D R H+ GRLA++IAK LL G VVVVRCE+I +SG R K+K M FLRK
Sbjct: 6 GICSKRVVVDARHHMCGRLASIIAKELLNGQSVVVVRCEEICLSGGLVRQKMKYMRFLRK 65
Query: 183 RCNVNPARGPFHFRAPSKILWKTV 254
R N P+ GP HFRAPSKI W+TV
Sbjct: 66 RMNTKPSHGPIHFRAPSKIFWRTV 89
Score = 79.0 bits (186), Expect = 2e-15
Identities = 34/76 (44%), Positives = 46/76 (60%)
Frame = +2
Query: 254 RGMIPHKTERGKNALRXLRTYDGCPPPFDNXXXXXXXXXXXXFCLKPGRNYCHVGRLSHE 433
RGMIPHKT+RG AL L+ ++G PPP+D L+ G YC +GRLS E
Sbjct: 90 RGMIPHKTKRGAAALARLKVFEGVPPPYDKVKRMVIPDALKVLRLQAGHKYCLLGRLSSE 149
Query: 434 IGWKYRDVVRKLEDKR 481
+GW + D +++LE KR
Sbjct: 150 VGWNHYDTIKELEVKR 165
>At3g07110.1 68416.m00847 60S ribosomal protein L13A (RPL13aA)
similar to ribosomal protein L13A GB:O49885 [Lupinus
luteus]
Length = 206
Score = 113 bits (271), Expect = 1e-25
Identities = 50/84 (59%), Positives = 61/84 (72%)
Frame = +3
Query: 3 GFSNKAIVIDGRGHLLGRLAAVIAKVLLEGNKVVVVRCEQINISGNFFRNKLKLMSFLRK 182
G K +V+D R H+LGRLA+V+AK LL G +VVVRCE+I +SG R K+K M FLRK
Sbjct: 6 GICAKRVVVDARHHMLGRLASVVAKDLLNGQNIVVVRCEEICLSGGLVRQKMKYMRFLRK 65
Query: 183 RCNVNPARGPFHFRAPSKILWKTV 254
R N P+ GP HFRAPSKI W+TV
Sbjct: 66 RMNTKPSHGPIHFRAPSKIFWRTV 89
Score = 81.4 bits (192), Expect = 4e-16
Identities = 37/90 (41%), Positives = 51/90 (56%)
Frame = +2
Query: 254 RGMIPHKTERGKNALRXLRTYDGCPPPFDNXXXXXXXXXXXXFCLKPGRNYCHVGRLSHE 433
RGMIPHKT+RG NAL L+ ++G P P+D L+ G YC +GRLS E
Sbjct: 90 RGMIPHKTKRGANALARLKVFEGVPTPYDKIKRMVVPDALKVLRLQAGHKYCLLGRLSSE 149
Query: 434 IGWKYRDVVRKLEDKRXGXAVXRVAMKRNL 523
+GW + D +++LE+KR A K+ L
Sbjct: 150 VGWNHYDTIKELENKRKERAQAVYERKKQL 179
>At4g13170.1 68417.m02049 60S ribosomal protein L13A (RPL13aC)
ribosomal protein L13a -Lupinus luteus,PID:e1237871
Length = 206
Score = 112 bits (269), Expect = 2e-25
Identities = 51/84 (60%), Positives = 61/84 (72%)
Frame = +3
Query: 3 GFSNKAIVIDGRGHLLGRLAAVIAKVLLEGNKVVVVRCEQINISGNFFRNKLKLMSFLRK 182
G K +V+DGR H+LGRLA+ AK LL G +VVVVRCE+I +SG R K+K M FLRK
Sbjct: 6 GICAKRVVVDGRHHMLGRLASNTAKELLNGQEVVVVRCEEICLSGGLVRQKMKYMRFLRK 65
Query: 183 RCNVNPARGPFHFRAPSKILWKTV 254
R N P+ GP HFRAPSKI W+TV
Sbjct: 66 RMNTKPSHGPIHFRAPSKIFWRTV 89
Score = 80.2 bits (189), Expect = 1e-15
Identities = 36/90 (40%), Positives = 50/90 (55%)
Frame = +2
Query: 254 RGMIPHKTERGKNALRXLRTYDGCPPPFDNXXXXXXXXXXXXFCLKPGRNYCHVGRLSHE 433
RGMIPHKT+RG AL L+ ++G PPP+D L+ G YC +GRLS E
Sbjct: 90 RGMIPHKTKRGAAALARLKVFEGIPPPYDKIKRMVIPDALKVLRLQSGHKYCLLGRLSSE 149
Query: 434 IGWKYRDVVRKLEDKRXGXAVXRVAMKRNL 523
+GW + D +++LE KR + K+ L
Sbjct: 150 VGWNHYDTIKELETKRKERSQVMYERKKQL 179
>At3g10270.1 68416.m01231 DNA topoisomerase, ATP-hydrolyzing,
putative / DNA topoisomerase II, putative / DNA gyrase,
putative similar to SP|O50627 DNA gyrase subunit B (EC
5.99.1.3) {Bacillus halodurans}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00204: DNA topoisomerase II
(N-terminal region), PF00986: DNA gyrase, B subunit,
carboxyl terminus, PF01751: Toprim domain
Length = 657
Score = 28.3 bits (60), Expect = 4.3
Identities = 15/52 (28%), Positives = 27/52 (51%)
Frame = +3
Query: 3 GFSNKAIVIDGRGHLLGRLAAVIAKVLLEGNKVVVVRCEQINISGNFFRNKL 158
G++N IDG H+ G A++ + K+ V++ + I++SG R L
Sbjct: 288 GYANSIRTIDGGTHIEGVKASLTRTLNSLAKKLKVIKEKDISLSGEHVREGL 339
>At5g11250.1 68418.m01314 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1189
Score = 27.5 bits (58), Expect = 7.5
Identities = 16/39 (41%), Positives = 25/39 (64%)
Frame = -1
Query: 244 HRILDGALKWKGPRAGFTLHLLRRNDISLSLFLKKLPEI 128
H LD + W+G + LH LR+ D+S S+ LK+LP++
Sbjct: 662 HSKLD--MLWEGVKP---LHNLRQMDLSYSVNLKELPDL 695
>At5g03330.2 68418.m00285 OTU-like cysteine protease family protein
contains Pfam profile PF02338: OTU-like cysteine
protease
Length = 356
Score = 27.5 bits (58), Expect = 7.5
Identities = 14/50 (28%), Positives = 25/50 (50%)
Frame = +3
Query: 147 RNKLKLMSFLRKRCNVNPARGPFHFRAPSKILWKTVEA*SHTKQSVARML 296
RN+L++ F + P G FRA + L+KT + H ++ + + L
Sbjct: 205 RNRLEMFDFTEVKV---PGDGNCQFRALADQLYKTADRHKHVRRQIVKQL 251
>At5g03330.1 68418.m00284 OTU-like cysteine protease family protein
contains Pfam profile PF02338: OTU-like cysteine
protease
Length = 356
Score = 27.5 bits (58), Expect = 7.5
Identities = 14/50 (28%), Positives = 25/50 (50%)
Frame = +3
Query: 147 RNKLKLMSFLRKRCNVNPARGPFHFRAPSKILWKTVEA*SHTKQSVARML 296
RN+L++ F + P G FRA + L+KT + H ++ + + L
Sbjct: 205 RNRLEMFDFTEVKV---PGDGNCQFRALADQLYKTADRHKHVRRQIVKQL 251
>At1g49260.1 68414.m05523 hypothetical protein
Length = 149
Score = 27.5 bits (58), Expect = 7.5
Identities = 10/28 (35%), Positives = 14/28 (50%)
Frame = -1
Query: 298 KSILATLCFVWDHASTVFHRILDGALKW 215
K+I+ T C W H +T RI + W
Sbjct: 64 KAIITTCCSAWFHLTTYQERIEESLFSW 91
>At4g27590.1 68417.m03963 copper-binding protein-related low
similarity to copper homeostasis factor gi:3168840 from
Arabidopsis thaliana; contains Pfam profile PF00403:
Heavy-metal-associated domain
Length = 156
Score = 27.1 bits (57), Expect = 9.9
Identities = 20/59 (33%), Positives = 24/59 (40%)
Frame = -1
Query: 409 TVITARFQTEDT*SSRHYNTSTVIKGRRAPIICT*PPKSILATLCFVWDHASTVFHRIL 233
T+I F T+ SS Y ST P PP S++ T F W VF R L
Sbjct: 103 TIIKTSFDTDK--SSAFYTYSTT-----PPRFSIRPPMSLIRTSSFTWKAVKKVFTRSL 154
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,832,333
Number of Sequences: 28952
Number of extensions: 276162
Number of successful extensions: 725
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 703
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 725
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1236350304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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