Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30393
(334 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g03850.1 68418.m00356 40S ribosomal protein S28 (RPS28B) ribo... 73 4e-14
At3g10090.1 68416.m01209 40S ribosomal protein S28 (RPS28A) simi... 73 4e-14
At5g64140.1 68418.m08054 40S ribosomal protein S28 (RPS28C) 72 8e-14
At2g43890.1 68415.m05456 polygalacturonase, putative / pectinase... 31 0.15
At1g07180.1 68414.m00764 pyridine nucleotide-disulphide oxidored... 31 0.25
At4g35500.2 68417.m05045 protein kinase family protein contains ... 29 0.77
At4g35500.1 68417.m05044 protein kinase family protein contains ... 29 1.0
At4g34140.1 68417.m04845 D111/G-patch domain-containing protein ... 27 2.4
At1g29590.1 68414.m03618 eukaryotic translation initiation facto... 27 3.1
At1g29550.1 68414.m03614 eukaryotic translation initiation facto... 27 3.1
At1g53470.1 68414.m06061 mechanosensitive ion channel domain-con... 27 4.1
At1g29820.1 68414.m03645 expressed protein 27 4.1
At5g19460.1 68418.m02319 MutT/nudix family protein similar to SP... 26 5.5
At4g32560.2 68417.m04635 paramyosin-related contains weak simila... 26 5.5
At4g32560.1 68417.m04634 paramyosin-related contains weak simila... 26 5.5
At1g20260.2 68414.m02530 vacuolar ATP synthase subunit B, putati... 26 5.5
At5g02930.1 68418.m00236 F-box family protein low similarity to ... 26 7.2
At4g08500.1 68417.m01401 mitogen-activated protein kinase kinase... 26 7.2
At3g19480.1 68416.m02469 D-3-phosphoglycerate dehydrogenase, put... 26 7.2
At2g21790.1 68415.m02590 ribonucleoside-diphosphate reductase sm... 26 7.2
At5g47910.1 68418.m05918 respiratory burst oxidase protein D (Rb... 25 9.5
At1g73580.1 68414.m08518 C2 domain-containing protein similar to... 25 9.5
>At5g03850.1 68418.m00356 40S ribosomal protein S28 (RPS28B)
ribosomal protein S28, Arabidopsis thaliana,
EMBL:ATRP28A
Length = 64
Score = 73.3 bits (172), Expect = 4e-14
Identities = 38/57 (66%), Positives = 46/57 (80%)
Frame = +3
Query: 84 MDKPNVLARVVKVLGRTGSQGQCTQVKVEFIGETSRQIIRNVKGPVRDGDILTLLES 254
MD A VVKV+GRTGS+GQ TQV+V+F ++ R I+RNVKGPVR+GDILTLLES
Sbjct: 1 MDSQIKHAVVVKVMGRTGSRGQVTQVRVKFT-DSDRYIMRNVKGPVREGDILTLLES 56
>At3g10090.1 68416.m01209 40S ribosomal protein S28 (RPS28A) similar
to ribosomal protein S28 GB:P34789 [Arabidopsis
thaliana]
Length = 64
Score = 73.3 bits (172), Expect = 4e-14
Identities = 38/57 (66%), Positives = 46/57 (80%)
Frame = +3
Query: 84 MDKPNVLARVVKVLGRTGSQGQCTQVKVEFIGETSRQIIRNVKGPVRDGDILTLLES 254
MD A VVKV+GRTGS+GQ TQV+V+F ++ R I+RNVKGPVR+GDILTLLES
Sbjct: 1 MDSQIKHAVVVKVMGRTGSRGQVTQVRVKFT-DSDRYIMRNVKGPVREGDILTLLES 56
>At5g64140.1 68418.m08054 40S ribosomal protein S28 (RPS28C)
Length = 64
Score = 72.1 bits (169), Expect = 8e-14
Identities = 37/57 (64%), Positives = 46/57 (80%)
Frame = +3
Query: 84 MDKPNVLARVVKVLGRTGSQGQCTQVKVEFIGETSRQIIRNVKGPVRDGDILTLLES 254
MD A VVKV+GRTGS+GQ TQV+V+F ++ R I+RNVKGPVR+GD+LTLLES
Sbjct: 1 MDSQIKHAVVVKVMGRTGSRGQVTQVRVKFT-DSDRFIMRNVKGPVREGDVLTLLES 56
>At2g43890.1 68415.m05456 polygalacturonase, putative / pectinase,
putative similar to SP|P48979 Polygalacturonase
precursor (EC 3.2.1.15) (PG) (Pectinase) {Prunus
persica}; contains Pfam profile PF00295: Glycosyl
hydrolases family 28 (polygalacturonases)
Length = 392
Score = 31.5 bits (68), Expect = 0.15
Identities = 17/39 (43%), Positives = 24/39 (61%)
Frame = -3
Query: 263 LHVRFKKSEDVSVTDGSFHVSDDLTAGLPNELDLHLSTL 147
LHV+ S V+VTDG+FH DD + P +L++S L
Sbjct: 199 LHVQ--GSAGVTVTDGTFHTGDDCISIGPGTRNLYMSKL 235
>At1g07180.1 68414.m00764 pyridine nucleotide-disulphide
oxidoreductase family protein contains similarity to
alternative NADH-dehydrogenase GI:3718005 from [Yarrowia
lipolytica], SP|P32340 Rotenone-insensitive
NADH-ubiquinone oxidoreductase, mitochondrial precursor
(EC 1.6.5.3) (Internal NADH dehydrogenase)
{Saccharomyces cerevisiae} ; contains Pfam profile
PF00070: Pyridine nucleotide-disulphide oxidoreductase
Length = 510
Score = 30.7 bits (66), Expect = 0.25
Identities = 19/50 (38%), Positives = 29/50 (58%), Gaps = 3/50 (6%)
Frame = +3
Query: 165 VEFIGETSRQIIRNVK---GPVRDGDILTLLESNVKLGGCDRLLQHYCIR 305
VEF GE S I+++V+ V+D +TL+E+ L D L+HY I+
Sbjct: 254 VEFSGELSDFIMKDVRQRYSHVKDDIRVTLIEARDILSSFDDRLRHYAIK 303
>At4g35500.2 68417.m05045 protein kinase family protein contains
eukaryotic protein kinase domain, INTERPRO:IPR000719
Length = 439
Score = 29.1 bits (62), Expect = 0.77
Identities = 15/64 (23%), Positives = 31/64 (48%)
Frame = +3
Query: 12 VFFSKHASFGARVLIALSSILQAKMDKPNVLARVVKVLGRTGSQGQCTQVKVEFIGETSR 191
+F + A + LS+ +DK + R++ +G GQ + +EF+G++
Sbjct: 71 LFAQQFAQAALHEIEFLSAAADGDLDKTKCVVRLIDHFKHSGPNGQHLCMVLEFLGDSLL 130
Query: 192 QIIR 203
++IR
Sbjct: 131 RLIR 134
>At4g35500.1 68417.m05044 protein kinase family protein contains
eukaryotic protein kinase domain, INTERPRO:IPR000719
Length = 438
Score = 28.7 bits (61), Expect = 1.0
Identities = 13/48 (27%), Positives = 26/48 (54%)
Frame = +3
Query: 60 LSSILQAKMDKPNVLARVVKVLGRTGSQGQCTQVKVEFIGETSRQIIR 203
LS+ +DK + R++ +G GQ + +EF+G++ ++IR
Sbjct: 86 LSAAADGDLDKTKCVVRLIDHFKHSGPNGQHLCMVLEFLGDSLLRLIR 133
>At4g34140.1 68417.m04845 D111/G-patch domain-containing protein
contains Pfam PF01585: G-patch domain
Length = 418
Score = 27.5 bits (58), Expect = 2.4
Identities = 11/34 (32%), Positives = 18/34 (52%)
Frame = -2
Query: 165 PSLEYTGPENQYGQALSRHEQERWVYPFWLVISS 64
P ++ E +G+A E+ERW+ + VI S
Sbjct: 134 PEEDFDPQEENFGEAAPSSEEERWLAQYGQVIES 167
>At1g29590.1 68414.m03618 eukaryotic translation initiation factor
4E, putative / eIF-4E, putative / eIF4E, putative / mRNA
cap-binding protein, putative similar to SP|O23252
Eukaryotic translation initiation factor 4E (eIF-4E)
(eIF4E) (mRNA cap-binding protein) (eIF-4F 25 kDa
subunit) (eIF-4F P26 subunit) {Arabidopsis thaliana};
contains Pfam profile PF01652: Eukaryotic initiation
factor 4E
Length = 285
Score = 27.1 bits (57), Expect = 3.1
Identities = 9/24 (37%), Positives = 14/24 (58%)
Frame = +2
Query: 110 CRESAWPYWFSGPVYSSEGRVHWG 181
C +++W +WF P S +V WG
Sbjct: 111 CFQNSWTFWFDNP-SSKSNQVIWG 133
>At1g29550.1 68414.m03614 eukaryotic translation initiation factor
4E, putative / eIF-4E, putative / eIF4E, putative / mRNA
cap-binding protein, putative similar to SP|O23252
Eukaryotic translation initiation factor 4E (eIF-4E)
(eIF4E) (mRNA cap-binding protein) (eIF-4F 25 kDa
subunit) (eIF-4F P26 subunit) {Arabidopsis thaliana};
contains Pfam profile PF01652: Eukaryotic initiation
factor 4E
Length = 240
Score = 27.1 bits (57), Expect = 3.1
Identities = 9/24 (37%), Positives = 14/24 (58%)
Frame = +2
Query: 110 CRESAWPYWFSGPVYSSEGRVHWG 181
C +++W +WF P S +V WG
Sbjct: 66 CFQNSWTFWFDNP-SSKSNQVIWG 88
>At1g53470.1 68414.m06061 mechanosensitive ion channel
domain-containing protein / MS ion channel
domain-containing protein contains Pfam profile PF00924:
Mechanosensitive ion channel
Length = 881
Score = 26.6 bits (56), Expect = 4.1
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = -1
Query: 277 SQPPSFTLDSRRVRMSPSRTG 215
S PP T+ S R++ SPSR G
Sbjct: 462 SSPPQVTVGSGRLQKSPSRVG 482
>At1g29820.1 68414.m03645 expressed protein
Length = 540
Score = 26.6 bits (56), Expect = 4.1
Identities = 10/30 (33%), Positives = 16/30 (53%)
Frame = -2
Query: 120 LSRHEQERWVYPFWLVISSLMQLTHVHQSW 31
+ R+E WV W I+ L+Q+ V+ W
Sbjct: 108 VERYEGSHWVPIGWARITELVQMVQVNAEW 137
>At5g19460.1 68418.m02319 MutT/nudix family protein similar to
SP|P41888 Thiamine pyrophosphokinase (EC 2.7.6.2) (TPK)
(Thiamine kinase) {Schizosaccharomyces pombe}; contains
Pfam profile PF00293: NUDIX domain
Length = 374
Score = 26.2 bits (55), Expect = 5.5
Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 2/42 (4%)
Frame = +3
Query: 18 FSKHASFGARV--LIALSSILQAKMDKPNVLARVVKVLGRTG 137
FS++ S RV + L+ +LQ D+ +A V+K+LG G
Sbjct: 128 FSQNGSCPDRVDGYVTLNLMLQKPEDRTRAVADVIKILGDKG 169
>At4g32560.2 68417.m04635 paramyosin-related contains weak
similarity to Paramyosin (Swiss-Prot:P10567)
[Caenorhabditis elegans]
Length = 306
Score = 26.2 bits (55), Expect = 5.5
Identities = 12/47 (25%), Positives = 23/47 (48%)
Frame = +3
Query: 150 CTQVKVEFIGETSRQIIRNVKGPVRDGDILTLLESNVKLGGCDRLLQ 290
C VK ++ E +++ N+K VR+ + ++ K D+L Q
Sbjct: 11 CLSVKEKYTSEQPEEMVTNLKASVRELSVKVKEQNQRKCDAKDKLQQ 57
>At4g32560.1 68417.m04634 paramyosin-related contains weak
similarity to Paramyosin (Swiss-Prot:P10567)
[Caenorhabditis elegans]
Length = 306
Score = 26.2 bits (55), Expect = 5.5
Identities = 12/47 (25%), Positives = 23/47 (48%)
Frame = +3
Query: 150 CTQVKVEFIGETSRQIIRNVKGPVRDGDILTLLESNVKLGGCDRLLQ 290
C VK ++ E +++ N+K VR+ + ++ K D+L Q
Sbjct: 11 CLSVKEKYTSEQPEEMVTNLKASVRELSVKVKEQNQRKCDAKDKLQQ 57
>At1g20260.2 68414.m02530 vacuolar ATP synthase subunit B, putative
/ V-ATPase B subunit, putative / vacuolar proton pump B
subunit, putative / V-ATPase 57 kDa subunit, putative
strong similarity to SP|P11574 Vacuolar ATP synthase
subunit B (EC 3.6.3.14) (V-ATPase B subunit) (Vacuolar
proton pump B subunit) (V-ATPase 57 kDa subunit)
{Arabidopsis thaliana}; contains Pfam profiles PF00006:
ATP synthase alpha/beta family nucleotide-binding
domain, PF02874: ATP synthase alpha/beta family
beta-barrel domain
Length = 485
Score = 26.2 bits (55), Expect = 5.5
Identities = 9/26 (34%), Positives = 13/26 (50%)
Frame = +2
Query: 98 RSCSCRESAWPYWFSGPVYSSEGRVH 175
R C R S+W W SG Y+ ++
Sbjct: 285 RFCCSRRSSWKTWISGVYYTDLATIY 310
>At5g02930.1 68418.m00236 F-box family protein low similarity to
ribosomal RNA apurinic site specific lyase [Triticum
aestivum] GI:6505722; contains F-box domain Pfam:PF00646
Length = 469
Score = 25.8 bits (54), Expect = 7.2
Identities = 17/42 (40%), Positives = 24/42 (57%)
Frame = -3
Query: 257 VRFKKSEDVSVTDGSFHVSDDLTAGLPNELDLHLSTLALRTS 132
V FK+S++VSV G +SD A L + + LA+RTS
Sbjct: 13 VMFKRSKNVSVDVGVDSISDLPDAVLQHIFSYIPTELAIRTS 54
>At4g08500.1 68417.m01401 mitogen-activated protein kinase kinase,
putative similar to mitogen-activated protein kinase
MEKK1 GP|1255448 [Arabidopsis thaliana]
Length = 608
Score = 25.8 bits (54), Expect = 7.2
Identities = 18/61 (29%), Positives = 28/61 (45%), Gaps = 4/61 (6%)
Frame = +3
Query: 135 GSQGQ-CTQV---KVEFIGETSRQIIRNVKGPVRDGDILTLLESNVKLGGCDRLLQHYCI 302
GSQ Q C Q +++ + + Q I +G +DG L + V G +L Q Y +
Sbjct: 369 GSQAQECIQQLEGEIKLLSQLQHQNIVRYRGTAKDGSNLYIFLELVTQGSLLKLYQRYQL 428
Query: 303 R 305
R
Sbjct: 429 R 429
>At3g19480.1 68416.m02469 D-3-phosphoglycerate dehydrogenase,
putative / 3-PGDH, putative similar to SP:O04130 from
[Arabidopsis thaliana]
Length = 588
Score = 25.8 bits (54), Expect = 7.2
Identities = 12/36 (33%), Positives = 21/36 (58%)
Frame = +3
Query: 21 SKHASFGARVLIALSSILQAKMDKPNVLARVVKVLG 128
+K FG V + S IL ++D+P ++ +V +LG
Sbjct: 501 TKVGLFGVDVSLEGSVILCRQVDQPGMIGKVASILG 536
>At2g21790.1 68415.m02590 ribonucleoside-diphosphate reductase small
chain, putative / ribonucleotide reductase, putative
similar to ribonucleotide reductase GI:4151068 from
[Nicotiana tabacum]
Length = 816
Score = 25.8 bits (54), Expect = 7.2
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = -3
Query: 305 PNAVMLQ*SIAAS*LHVRFKKSEDVSVTDGSFHVSD 198
P+ L IA S LH KKS ++ D +HV+D
Sbjct: 72 PDYASLAARIAVSNLHKNTKKSFSETIKDMFYHVND 107
>At5g47910.1 68418.m05918 respiratory burst oxidase protein D
(RbohD) / NADPH oxidase identical to respiratory burst
oxidase protein D from Arabidopsis thaliana [gi:3242789]
Length = 921
Score = 25.4 bits (53), Expect = 9.5
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = +3
Query: 189 RQIIRNVKGPVRDGDI 236
+ II N+KGP RD DI
Sbjct: 756 KDIINNMKGPDRDSDI 771
>At1g73580.1 68414.m08518 C2 domain-containing protein similar to
zinc finger and C2 domain protein GI:9957238 from
[Arabidopsis thaliana]
Length = 168
Score = 25.4 bits (53), Expect = 9.5
Identities = 19/52 (36%), Positives = 30/52 (57%)
Frame = +3
Query: 159 VKVEFIGETSRQIIRNVKGPVRDGDILTLLESNVKLGGCDRLLQHYCIRLRH 314
+++E G II + GP R G+ L ES ++ DR++QH C+RLR+
Sbjct: 100 LRMELSGLPDGTIISTI-GPSR-GNCLAE-ESYIRWIN-DRIVQHICLRLRN 147
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,956,342
Number of Sequences: 28952
Number of extensions: 128963
Number of successful extensions: 361
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 355
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 358
length of database: 12,070,560
effective HSP length: 71
effective length of database: 10,014,968
effective search space used: 390583752
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -