Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30375
(500 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g35350.2 68417.m05022 cysteine endopeptidase, papain-type (XC... 33 0.14
At4g35350.1 68417.m05023 cysteine endopeptidase, papain-type (XC... 33 0.14
At1g29110.1 68414.m03563 cysteine proteinase, putative contains ... 32 0.19
At5g60360.1 68418.m07568 cysteine proteinase, putative / AALP pr... 30 1.0
At2g34080.1 68415.m04172 cysteine proteinase, putative contains ... 29 1.3
At1g47128.1 68414.m05222 cysteine proteinase (RD21A) / thiol pro... 29 2.3
At4g11310.1 68417.m01827 cysteine proteinase, putative contains ... 28 3.1
At2g42920.1 68415.m05318 pentatricopeptide (PPR) repeat-containi... 28 3.1
At2g27420.1 68415.m03314 cysteine proteinase, putative contains ... 28 4.1
At4g10790.1 68417.m01759 UBX domain-containing protein low simil... 27 7.1
At4g31420.2 68417.m04461 zinc finger (C2H2 type) family protein ... 27 9.4
At4g31420.1 68417.m04460 zinc finger (C2H2 type) family protein ... 27 9.4
At2g47860.2 68415.m05974 phototropic-responsive NPH3 family prot... 27 9.4
At2g47860.1 68415.m05973 phototropic-responsive NPH3 family prot... 27 9.4
>At4g35350.2 68417.m05022 cysteine endopeptidase, papain-type (XCP1)
identical to papain-type cysteine endopeptidase XCP1
GI:6708181 from [Arabidopsis thaliana]
Length = 288
Score = 32.7 bits (71), Expect = 0.14
Identities = 18/63 (28%), Positives = 33/63 (52%), Gaps = 1/63 (1%)
Frame = +1
Query: 70 ALLIATVVMASSAETDTPRHY-DLNQAKELFEIFVKEHNREYKDDADRELHYQSFKKHLA 246
ALL S TP H + ++ ELFE ++ EH++ YK ++ ++ F+++L
Sbjct: 21 ALLCCAFARDFSIVGYTPEHLTNTDKLLELFESWMSEHSKAYKSVEEKVHRFEVFRENLM 80
Query: 247 EIN 255
I+
Sbjct: 81 HID 83
Score = 30.3 bits (65), Expect = 0.76
Identities = 18/36 (50%), Positives = 23/36 (63%), Gaps = 1/36 (2%)
Frame = +3
Query: 249 DKQLNEKNPYTTFGINKFADYTPEEQQSR-LGLRLP 353
D++ NE N Y G+N+FAD T EE + R LGL P
Sbjct: 83 DQRNNEINSYW-LGLNEFADLTHEEFKGRYLGLAKP 117
>At4g35350.1 68417.m05023 cysteine endopeptidase, papain-type (XCP1)
identical to papain-type cysteine endopeptidase XCP1
GI:6708181 from [Arabidopsis thaliana]
Length = 355
Score = 32.7 bits (71), Expect = 0.14
Identities = 18/63 (28%), Positives = 33/63 (52%), Gaps = 1/63 (1%)
Frame = +1
Query: 70 ALLIATVVMASSAETDTPRHY-DLNQAKELFEIFVKEHNREYKDDADRELHYQSFKKHLA 246
ALL S TP H + ++ ELFE ++ EH++ YK ++ ++ F+++L
Sbjct: 21 ALLCCAFARDFSIVGYTPEHLTNTDKLLELFESWMSEHSKAYKSVEEKVHRFEVFRENLM 80
Query: 247 EIN 255
I+
Sbjct: 81 HID 83
Score = 30.3 bits (65), Expect = 0.76
Identities = 18/36 (50%), Positives = 23/36 (63%), Gaps = 1/36 (2%)
Frame = +3
Query: 249 DKQLNEKNPYTTFGINKFADYTPEEQQSR-LGLRLP 353
D++ NE N Y G+N+FAD T EE + R LGL P
Sbjct: 83 DQRNNEINSYW-LGLNEFADLTHEEFKGRYLGLAKP 117
>At1g29110.1 68414.m03563 cysteine proteinase, putative contains
similarity to cysteine protease SPCP1 GI:13491750 from
[Ipomoea batatas]
Length = 334
Score = 32.3 bits (70), Expect = 0.19
Identities = 19/70 (27%), Positives = 39/70 (55%), Gaps = 4/70 (5%)
Frame = +1
Query: 61 VSV-ALLIATVVMASSAETDTPR-HYDLNQAK--ELFEIFVKEHNREYKDDADRELHYQS 228
VSV ++ +A +++ R H LN+ + + ++ + +R YKD++++E+ +
Sbjct: 2 VSVRSVFVALTILSMDLRISQARPHVTLNEQSIVDYHQQWMTQFSRVYKDESEKEMRLKV 61
Query: 229 FKKHLAEINN 258
FKK+L I N
Sbjct: 62 FKKNLKFIEN 71
>At5g60360.1 68418.m07568 cysteine proteinase, putative / AALP
protein (AALP) identical to AALP protein GI:7230640 from
[Arabidopsis thaliana]; similar to barley aleurain
Length = 358
Score = 29.9 bits (64), Expect = 1.0
Identities = 16/41 (39%), Positives = 25/41 (60%), Gaps = 1/41 (2%)
Frame = +3
Query: 222 SIIQETLGRDKQLNEKNPYTTFGINKFADYTPEE-QQSRLG 341
SI +E L + N+K G+N+FAD T +E Q+++LG
Sbjct: 81 SIFKENLDLIRSTNKKGLSYKLGVNQFADLTWQEFQRTKLG 121
>At2g34080.1 68415.m04172 cysteine proteinase, putative contains
similarity to cysteine protease SPCP1 GI:13491750 from
[Ipomoea batatas]
Length = 345
Score = 29.5 bits (63), Expect = 1.3
Identities = 18/69 (26%), Positives = 35/69 (50%)
Frame = +1
Query: 52 MNFVSVALLIATVVMASSAETDTPRHYDLNQAKELFEIFVKEHNREYKDDADRELHYQSF 231
M V+V +++ T S A + T + + + E ++ +REY+D+ ++ + F
Sbjct: 5 MVLVTVLIILFTGFRISQATSRTVIFREQSMVDK-HEQWMARFSREYRDELEKNMRRDVF 63
Query: 232 KKHLAEINN 258
KK+L I N
Sbjct: 64 KKNLKFIEN 72
>At1g47128.1 68414.m05222 cysteine proteinase (RD21A) / thiol
protease identical to SP|P43297 Cysteine proteinase
RD21A precursor (EC 3.4.22.-) {Arabidopsis thaliana},
thiol protease RD21A SP:P43297 from [Arabidopsis
thaliana]
Length = 462
Score = 28.7 bits (61), Expect = 2.3
Identities = 16/46 (34%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +3
Query: 225 IIQETLGRDKQLNEKNPYTTFGINKFADYTPEEQQSR-LGLRLPAK 359
I ++ L + NEKN G+ +FAD T +E +S+ LG ++ K
Sbjct: 75 IFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKK 120
>At4g11310.1 68417.m01827 cysteine proteinase, putative contains
similarity to cysteine proteinase RD21A (thiol protease)
GI:435619, SP:P43297 from [Arabidopsis thaliana]
Length = 364
Score = 28.3 bits (60), Expect = 3.1
Identities = 22/84 (26%), Positives = 40/84 (47%)
Frame = +1
Query: 7 LGTSKPVPLLIHCAKMNFVSVALLIATVVMASSAETDTPRHYDLNQAKELFEIFVKEHNR 186
+G++K L++ A M S A I V++ +D +A +FE ++ +H +
Sbjct: 1 MGSAKSAMLILLVA-MVIASCATAIDMSVVSYDDNNRLHSVFDA-EASLIFESWMVKHGK 58
Query: 187 EYKDDADRELHYQSFKKHLAEINN 258
Y A++E F+ +L INN
Sbjct: 59 VYGSVAEKERRLTIFEDNLRFINN 82
>At2g42920.1 68415.m05318 pentatricopeptide (PPR) repeat-containing
protein and genefinder
Length = 559
Score = 28.3 bits (60), Expect = 3.1
Identities = 16/43 (37%), Positives = 24/43 (55%)
Frame = -2
Query: 409 FVWNSIPID*SRRTYVFLAGSLNPNLLCCSSGV*SANLLMPNV 281
FVWN+I SR ++ +A S+ ++LC S V L P+V
Sbjct: 90 FVWNTIIRGFSRSSFPEMAISIFIDMLCSSPSVKPQRLTYPSV 132
>At2g27420.1 68415.m03314 cysteine proteinase, putative contains
similarity to cysteine protease SPCP1 GI:13491750 from
[Ipomoea batatas]
Length = 348
Score = 27.9 bits (59), Expect = 4.1
Identities = 17/66 (25%), Positives = 29/66 (43%), Gaps = 1/66 (1%)
Frame = +1
Query: 64 SVALLIATVVMASSAETDTPRHYDLN-QAKELFEIFVKEHNREYKDDADRELHYQSFKKH 240
S + I T+ ++ T R A E E ++ NR Y D+ ++ + FKK+
Sbjct: 3 STIIFILTIFLSYRTSLATSRGSLFEASAIEKHEQWMARFNRVYSDETEKRNRFNIFKKN 62
Query: 241 LAEINN 258
L + N
Sbjct: 63 LEFVQN 68
>At4g10790.1 68417.m01759 UBX domain-containing protein low
similarity to SP|Q9UNN5 FAS-associated factor 1 (FAF1
protein) {Homo sapiens}; contains Pfam profile PF00789:
UBX domain
Length = 480
Score = 27.1 bits (57), Expect = 7.1
Identities = 13/42 (30%), Positives = 25/42 (59%)
Frame = -3
Query: 423 HNTFISFGIAFR*TEVAELMSS*QAASIPTCSAVPQEYNQQI 298
+ F+S+G + R +E ++ +S +A+ P C+ V NQ+I
Sbjct: 234 NENFVSWGGSIRSSEGFKMSNSLKASRFPFCAVVMPAANQRI 275
>At4g31420.2 68417.m04461 zinc finger (C2H2 type) family protein
contains Pfam profile: PF00096 zinc finger, C2H2 type
Length = 405
Score = 26.6 bits (56), Expect = 9.4
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +1
Query: 181 NREYKDDADRELHYQS 228
N E+KD+ +R LHY+S
Sbjct: 10 NMEFKDEEERNLHYKS 25
>At4g31420.1 68417.m04460 zinc finger (C2H2 type) family protein
contains Pfam profile: PF00096 zinc finger, C2H2 type
Length = 404
Score = 26.6 bits (56), Expect = 9.4
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +1
Query: 181 NREYKDDADRELHYQS 228
N E+KD+ +R LHY+S
Sbjct: 10 NMEFKDEEERNLHYKS 25
>At2g47860.2 68415.m05974 phototropic-responsive NPH3 family protein
contains NPH3 family domain, Pfam:PF03000
Length = 517
Score = 26.6 bits (56), Expect = 9.4
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = +1
Query: 130 YDLNQAKELFEIFVKEHNREYKDDADRE 213
YD + + +F +F+ EY+DD D E
Sbjct: 221 YDTDSVQRIFSMFLNLDECEYRDDDDDE 248
>At2g47860.1 68415.m05973 phototropic-responsive NPH3 family protein
contains NPH3 family domain, Pfam:PF03000
Length = 635
Score = 26.6 bits (56), Expect = 9.4
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = +1
Query: 130 YDLNQAKELFEIFVKEHNREYKDDADRE 213
YD + + +F +F+ EY+DD D E
Sbjct: 339 YDTDSVQRIFSMFLNLDECEYRDDDDDE 366
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,662,489
Number of Sequences: 28952
Number of extensions: 178796
Number of successful extensions: 466
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 453
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 465
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 888318720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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