BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30371 (646 letters) Database: uniref50 1,657,284 sequences; 575,637,011 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value UniRef50_UPI00003BFA45 Cluster: PREDICTED: similar to Nidogen/en... 63 6e-09 UniRef50_UPI0000D55DA8 Cluster: PREDICTED: similar to CG12908-PA... 51 3e-05 UniRef50_UPI0000F1F8DC Cluster: PREDICTED: similar to Nidogen-1 ... 46 8e-04 UniRef50_A6QNS6 Cluster: NID1 protein; n=5; Euteleostomi|Rep: NI... 44 0.003 UniRef50_P14543 Cluster: Nidogen-1 precursor; n=28; Tetrapoda|Re... 44 0.004 UniRef50_A1Z876 Cluster: CG12908-PB, isoform B; n=2; Drosophila ... 42 0.010 UniRef50_Q4RPY1 Cluster: Chromosome 17 SCAF15006, whole genome s... 40 0.039 UniRef50_Q04901 Cluster: Entactin/nidogen; n=1; Halocynthia rore... 40 0.051 UniRef50_Q7Q440 Cluster: ENSANGP00000021850; n=1; Anopheles gamb... 38 0.16 UniRef50_Q172X3 Cluster: Nidogen; n=1; Aedes aegypti|Rep: Nidoge... 38 0.16 UniRef50_Q93791 Cluster: Putative uncharacterized protein nid-1;... 37 0.36 UniRef50_UPI0000584B22 Cluster: PREDICTED: similar to Low-densit... 36 0.84 UniRef50_Q6XYD3 Cluster: LP2570; n=1; Homo sapiens|Rep: LP2570 -... 35 1.5 UniRef50_UPI00006A008C Cluster: UPI00006A008C related cluster; n... 34 2.6 UniRef50_A7S9Z7 Cluster: Predicted protein; n=1; Nematostella ve... 34 2.6 UniRef50_Q8IKQ4 Cluster: Putative uncharacterized protein; n=4; ... 34 3.4 UniRef50_UPI0000ECAC64 Cluster: Stabilin-1 precursor (Fasciclin,... 33 5.9 UniRef50_A5V5G9 Cluster: TonB-dependent receptor precursor; n=1;... 33 5.9 UniRef50_Q22AZ6 Cluster: Putative uncharacterized protein; n=1; ... 33 5.9 UniRef50_A7S7T4 Cluster: Predicted protein; n=1; Nematostella ve... 33 5.9 UniRef50_A6SE27 Cluster: Predicted protein; n=1; Botryotinia fuc... 33 5.9 >UniRef50_UPI00003BFA45 Cluster: PREDICTED: similar to Nidogen/entactin CG12908-PA, isoform A; n=1; Apis mellifera|Rep: PREDICTED: similar to Nidogen/entactin CG12908-PA, isoform A - Apis mellifera Length = 1263 Score = 62.9 bits (146), Expect = 6e-09 Identities = 31/73 (42%), Positives = 39/73 (53%), Gaps = 2/73 (2%) Frame = +1 Query: 1 RTLKIEIIDTITQARGQ-IDIPRS-TIRLYGVANAPSECPGAANVCSQRNGNCNAGQLCL 174 +T KIE+ T R Q I +P + +LYG+ P CP N C G C+ QLCL Sbjct: 1181 KTHKIEVAMKSTGDRKQAISVPPGGSGKLYGIVVVPESCPRVTNACQFEGGRCSKNQLCL 1240 Query: 175 PNGSGGRSCLNGD 213 PNG GRSC+ D Sbjct: 1241 PNGQNGRSCVCAD 1253 >UniRef50_UPI0000D55DA8 Cluster: PREDICTED: similar to CG12908-PA, isoform A; n=1; Tribolium castaneum|Rep: PREDICTED: similar to CG12908-PA, isoform A - Tribolium castaneum Length = 1320 Score = 50.8 bits (116), Expect = 3e-05 Identities = 19/52 (36%), Positives = 28/52 (53%) Frame = +1 Query: 49 QIDIPRSTIRLYGVANAPSECPGAANVCSQRNGNCNAGQLCLPNGSGGRSCL 204 Q+ + S +L+G+ P CP NVC C +CLPNG+G R+C+ Sbjct: 1254 QVPLGGSGNKLFGLVAVPQSCPQLTNVCQYYKDQCPVDHICLPNGAGSRTCV 1305 >UniRef50_UPI0000F1F8DC Cluster: PREDICTED: similar to Nidogen-1 precursor (Entactin); n=1; Danio rerio|Rep: PREDICTED: similar to Nidogen-1 precursor (Entactin) - Danio rerio Length = 942 Score = 46.0 bits (104), Expect = 8e-04 Identities = 23/66 (34%), Positives = 32/66 (48%), Gaps = 2/66 (3%) Frame = +1 Query: 10 KIEIIDTITQARGQIDI--PRSTIRLYGVANAPSECPGAANVCSQRNGNCNAGQLCLPNG 183 ++ ++ R ++D P+ RLYG+ A + CP N CS NG C LCL Sbjct: 865 RVSVVVVDRHLRREVDEFQPQKRTRLYGITTAHARCPAGQNYCSSNNGGCT--HLCLAT- 921 Query: 184 SGGRSC 201 GRSC Sbjct: 922 PAGRSC 927 >UniRef50_A6QNS6 Cluster: NID1 protein; n=5; Euteleostomi|Rep: NID1 protein - Bos taurus (Bovine) Length = 1241 Score = 44.0 bits (99), Expect = 0.003 Identities = 21/47 (44%), Positives = 25/47 (53%) Frame = +1 Query: 61 PRSTIRLYGVANAPSECPGAANVCSQRNGNCNAGQLCLPNGSGGRSC 201 P RLYG+ +A S+CP N CS NG C LCL G R+C Sbjct: 1183 PHKQTRLYGITSALSQCPEGHNYCSVNNGGCT--HLCLAT-PGSRTC 1226 >UniRef50_P14543 Cluster: Nidogen-1 precursor; n=28; Tetrapoda|Rep: Nidogen-1 precursor - Homo sapiens (Human) Length = 1247 Score = 43.6 bits (98), Expect = 0.004 Identities = 21/47 (44%), Positives = 24/47 (51%) Frame = +1 Query: 61 PRSTIRLYGVANAPSECPGAANVCSQRNGNCNAGQLCLPNGSGGRSC 201 P RLYG+ A S+CP N CS NG C LCL G R+C Sbjct: 1189 PHKQTRLYGITTALSQCPQGHNYCSVNNGGCT--HLCLAT-PGSRTC 1232 >UniRef50_A1Z876 Cluster: CG12908-PB, isoform B; n=2; Drosophila melanogaster|Rep: CG12908-PB, isoform B - Drosophila melanogaster (Fruit fly) Length = 1370 Score = 42.3 bits (95), Expect = 0.010 Identities = 22/69 (31%), Positives = 34/69 (49%), Gaps = 3/69 (4%) Frame = +1 Query: 4 TLKIEIIDTITQARGQIDIPR-STIRLYGVANAPSECPGAANVCSQRNGNCNAGQLCLPN 180 T K+EI+D++ + I P + ++YG+ CP + C NG C +LCL N Sbjct: 1286 TKKVEIVDSLGARQTPIQPPFFGSHKMYGMTVVEQHCPQYQSPCQISNGGCTDSRLCLVN 1345 Query: 181 --GSGGRSC 201 G+SC Sbjct: 1346 RQAPSGKSC 1354 >UniRef50_Q4RPY1 Cluster: Chromosome 17 SCAF15006, whole genome shotgun sequence; n=1; Tetraodon nigroviridis|Rep: Chromosome 17 SCAF15006, whole genome shotgun sequence - Tetraodon nigroviridis (Green puffer) Length = 1396 Score = 40.3 bits (90), Expect = 0.039 Identities = 19/47 (40%), Positives = 24/47 (51%) Frame = +1 Query: 61 PRSTIRLYGVANAPSECPGAANVCSQRNGNCNAGQLCLPNGSGGRSC 201 P+ +LYG+ ++CP N CS NG C LCL GRSC Sbjct: 1339 PQKRTKLYGLTTTYAQCPSGQNYCSVNNGGCT--HLCLATPL-GRSC 1382 >UniRef50_Q04901 Cluster: Entactin/nidogen; n=1; Halocynthia roretzi|Rep: Entactin/nidogen - Halocynthia roretzi (Sea squirt) Length = 1161 Score = 39.9 bits (89), Expect = 0.051 Identities = 22/48 (45%), Positives = 26/48 (54%), Gaps = 1/48 (2%) Frame = +1 Query: 61 PRSTI-RLYGVANAPSECPGAANVCSQRNGNCNAGQLCLPNGSGGRSC 201 PR T + YG+A S+CP N C Q NG C+ LCLP GR C Sbjct: 1105 PRGTHGQTYGIAVVHSQCPRGYNYCGQDNGGCS--HLCLPT-PYGRVC 1149 >UniRef50_Q7Q440 Cluster: ENSANGP00000021850; n=1; Anopheles gambiae str. PEST|Rep: ENSANGP00000021850 - Anopheles gambiae str. PEST Length = 1289 Score = 38.3 bits (85), Expect = 0.16 Identities = 22/69 (31%), Positives = 33/69 (47%), Gaps = 3/69 (4%) Frame = +1 Query: 4 TLKIEIIDTITQARGQIDIPR-STIRLYGVANAPSECPGAANVCSQRNGNCNAGQLCL-- 174 T KIE I+ + I+ P ++YG+ +CP + C NG+C ++CL Sbjct: 1207 TKKIESINLYGVRQKPINSPVFGNHKMYGMTAVTDKCPLFHSPCVSNNGDCPEDKICLIN 1266 Query: 175 PNGSGGRSC 201 P GRSC Sbjct: 1267 PRAPSGRSC 1275 >UniRef50_Q172X3 Cluster: Nidogen; n=1; Aedes aegypti|Rep: Nidogen - Aedes aegypti (Yellowfever mosquito) Length = 1298 Score = 38.3 bits (85), Expect = 0.16 Identities = 22/75 (29%), Positives = 33/75 (44%), Gaps = 3/75 (4%) Frame = +1 Query: 4 TLKIEIIDTITQARGQIDIPR-STIRLYGVANAPSECPGAANVCSQRNGNCNAGQLCL-- 174 T K+E I+ + I P ++YG+ +CP + C NG+C ++CL Sbjct: 1215 TKKVESINAYGEREKGIPSPVFGNHKMYGMTAVTDKCPLFYSPCVVNNGDCTEKRICLSN 1274 Query: 175 PNGSGGRSCLNGDAN 219 P GR C D N Sbjct: 1275 PRAPSGRGCKCADDN 1289 >UniRef50_Q93791 Cluster: Putative uncharacterized protein nid-1; n=2; Caenorhabditis|Rep: Putative uncharacterized protein nid-1 - Caenorhabditis elegans Length = 1584 Score = 37.1 bits (82), Expect = 0.36 Identities = 16/42 (38%), Positives = 22/42 (52%) Frame = +1 Query: 76 RLYGVANAPSECPGAANVCSQRNGNCNAGQLCLPNGSGGRSC 201 +++G+ P C G + CS+ NG C LCLP G G C Sbjct: 1535 KVFGILAVPKSCVGPSTPCSEDNGGCQ--HLCLP-GQNGAVC 1573 >UniRef50_UPI0000584B22 Cluster: PREDICTED: similar to Low-density lipoprotein receptor-related protein 4 precursor (Multiple epidermal growth factor-like domains 7); n=1; Strongylocentrotus purpuratus|Rep: PREDICTED: similar to Low-density lipoprotein receptor-related protein 4 precursor (Multiple epidermal growth factor-like domains 7) - Strongylocentrotus purpuratus Length = 948 Score = 35.9 bits (79), Expect = 0.84 Identities = 23/56 (41%), Positives = 27/56 (48%) Frame = +1 Query: 46 GQIDIPRSTIRLYGVANAPSECPGAANVCSQRNGNCNAGQLCLPNGSGGRSCLNGD 213 G+ D R L+ V NA P N CS+ NG C+ LCLP GRSC D Sbjct: 294 GKRDFTRPNA-LFAVTNA--SLPSGINSCSENNGGCST--LCLPTAI-GRSCACAD 343 >UniRef50_Q6XYD3 Cluster: LP2570; n=1; Homo sapiens|Rep: LP2570 - Homo sapiens (Human) Length = 117 Score = 35.1 bits (77), Expect = 1.5 Identities = 22/68 (32%), Positives = 30/68 (44%) Frame = -3 Query: 218 LASPFRQLLPPLPLGRHSCPALQLPFLCEQTLAAPGHSLGALATPYNRIVDLGMSICPLA 39 L P L PLP +HSC Q + PG G A+P ++I G + LA Sbjct: 2 LPQPLEFTLTPLPSAQHSCLKFQPNHRKQLLCEGPGTGRGWQASPNSQIAVTGYDLTSLA 61 Query: 38 WVIVSMIS 15 VS++S Sbjct: 62 RSRVSLLS 69 >UniRef50_UPI00006A008C Cluster: UPI00006A008C related cluster; n=3; Xenopus tropicalis|Rep: UPI00006A008C UniRef100 entry - Xenopus tropicalis Length = 1403 Score = 34.3 bits (75), Expect = 2.6 Identities = 15/35 (42%), Positives = 19/35 (54%) Frame = +1 Query: 100 PSECPGAANVCSQRNGNCNAGQLCLPNGSGGRSCL 204 PS P +N C NG C+ QLCLP R+C+ Sbjct: 928 PSRQPQGSNPCQINNGGCS--QLCLPTSETTRTCM 960 >UniRef50_A7S9Z7 Cluster: Predicted protein; n=1; Nematostella vectensis|Rep: Predicted protein - Nematostella vectensis Length = 1137 Score = 34.3 bits (75), Expect = 2.6 Identities = 13/26 (50%), Positives = 16/26 (61%) Frame = +1 Query: 124 NVCSQRNGNCNAGQLCLPNGSGGRSC 201 ++C NG C+A LCL G G RSC Sbjct: 647 DLCQVDNGGCHANALCLKTGPGKRSC 672 >UniRef50_Q8IKQ4 Cluster: Putative uncharacterized protein; n=4; Plasmodium|Rep: Putative uncharacterized protein - Plasmodium falciparum (isolate 3D7) Length = 553 Score = 33.9 bits (74), Expect = 3.4 Identities = 25/80 (31%), Positives = 35/80 (43%), Gaps = 1/80 (1%) Frame = +2 Query: 359 NVIHVD*PLFKINYCFSISKLQIKFETLLIEYIYVRFFNRYSINIQV*L*KWTDLNNLK- 535 N V+ L + + F +K QI F+ + Y V N S + KW LN LK Sbjct: 420 NASSVNKNLENLYFDFPYNKNQIIFKPVFYPYKNVEIINN-SFQNYIKNIKWDKLNKLKD 478 Query: 536 ITFFYHVTTKTFNLFKRVNI 595 F+Y+ K FN K + I Sbjct: 479 AQFYYNTKNKIFNFSKEIYI 498 >UniRef50_UPI0000ECAC64 Cluster: Stabilin-1 precursor (Fasciclin, EGF-like, laminin-type EGF-like and link domain-containing scavenger receptor 1) (FEEL-1) (MS-1 antigen).; n=2; Gallus gallus|Rep: Stabilin-1 precursor (Fasciclin, EGF-like, laminin-type EGF-like and link domain-containing scavenger receptor 1) (FEEL-1) (MS-1 antigen). - Gallus gallus Length = 2291 Score = 33.1 bits (72), Expect = 5.9 Identities = 16/39 (41%), Positives = 19/39 (48%) Frame = +1 Query: 85 GVANAPSECPGAANVCSQRNGNCNAGQLCLPNGSGGRSC 201 G A C A N C NG C+ +C+P G G RSC Sbjct: 940 GYAGDGHNCD-AINPCLMDNGGCHDLAICVPLGGGERSC 977 >UniRef50_A5V5G9 Cluster: TonB-dependent receptor precursor; n=1; Sphingomonas wittichii RW1|Rep: TonB-dependent receptor precursor - Sphingomonas wittichii RW1 Length = 809 Score = 33.1 bits (72), Expect = 5.9 Identities = 15/54 (27%), Positives = 26/54 (48%) Frame = -1 Query: 253 ADNYSLSAKVNSWRHRSDNSFPHFHWVGTAVRRCSYRFSASKRWQPRDTRSARW 92 +D +L+ VN+W+ +SD P F + + A++ + P D R A W Sbjct: 246 SDRLTLNLNVNAWQDKSDPQAPQFFGLQSQAPATLPPLIAAQPFTPHDARLAEW 299 >UniRef50_Q22AZ6 Cluster: Putative uncharacterized protein; n=1; Tetrahymena thermophila SB210|Rep: Putative uncharacterized protein - Tetrahymena thermophila SB210 Length = 1326 Score = 33.1 bits (72), Expect = 5.9 Identities = 18/51 (35%), Positives = 25/51 (49%) Frame = -1 Query: 598 SNINPFKKVKCFRSDVVKKRNFQII*VCPFLQLNLNINTIPIKKSNVNVFY 446 + + F + K R+ + KK+N QII P L I +KKS N FY Sbjct: 1245 NQLKTFMRWKLLRNSIKKKKNEQIIINIPQLDRTRLTENISLKKSKSNFFY 1295 >UniRef50_A7S7T4 Cluster: Predicted protein; n=1; Nematostella vectensis|Rep: Predicted protein - Nematostella vectensis Length = 330 Score = 33.1 bits (72), Expect = 5.9 Identities = 12/25 (48%), Positives = 15/25 (60%) Frame = +3 Query: 18 NHRHNNPGERANRHSKIDDTVIRCC 92 NHRHNN R++RHS D + C Sbjct: 168 NHRHNNNNNRSSRHSTADSAIDSIC 192 >UniRef50_A6SE27 Cluster: Predicted protein; n=1; Botryotinia fuckeliana B05.10|Rep: Predicted protein - Botryotinia fuckeliana B05.10 Length = 238 Score = 33.1 bits (72), Expect = 5.9 Identities = 20/63 (31%), Positives = 29/63 (46%) Frame = +1 Query: 13 IEIIDTITQARGQIDIPRSTIRLYGVANAPSECPGAANVCSQRNGNCNAGQLCLPNGSGG 192 I +I T +P T +G +N S C G A +C GN ++G C+ GS G Sbjct: 91 IALITTSANMEDYPYVPSQTTAGHGYSN--SACGGGAGICIPSGGNDDSG-FCVAAGSCG 147 Query: 193 RSC 201 +C Sbjct: 148 ITC 150 Database: uniref50 Posted date: Oct 5, 2007 11:19 AM Number of letters in database: 575,637,011 Number of sequences in database: 1,657,284 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 613,049,715 Number of Sequences: 1657284 Number of extensions: 12384777 Number of successful extensions: 32563 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 30345 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 32481 length of database: 575,637,011 effective HSP length: 97 effective length of database: 414,880,463 effective search space used: 48541014171 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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