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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS30370
         (676 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC25H2.05 |egd2|nac1|nascent polypeptide-associated complex al...    71   1e-13
SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces pomb...    28   1.4  
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos...    27   2.5  
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|...    25   10.0 

>SPBC25H2.05 |egd2|nac1|nascent polypeptide-associated complex alpha
           subunit Egd2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 173

 Score = 71.3 bits (167), Expect = 1e-13
 Identities = 36/80 (45%), Positives = 54/80 (67%)
 Frame = +1

Query: 235 TVSW*EEARKIMSKLGLKPVQGVERVTIRKSKNILFVINSPDVYKNPHSDTYIVFGEAKI 414
           TV   E+A+K++ KLGLK V+G+ RV +R+ KNIL +IN P VYK+  ++ YIV G+  +
Sbjct: 20  TVVHAEKAQKLIQKLGLKRVEGITRVAMRRPKNILLIINEPIVYKS-SNNAYIVLGKVTV 78

Query: 415 EDLSTQATMAAAERFKAPET 474
           ED++ QA      + +A ET
Sbjct: 79  EDMAAQARAFNESQKQATET 98



 Score = 35.1 bits (77), Expect = 0.009
 Identities = 17/30 (56%), Positives = 23/30 (76%)
 Frame = +3

Query: 585 IVMSQANVSRAKAVRALKNNHLTLLMQLWS 674
           +VM+QANVSRAKAV ALK N+  ++  + S
Sbjct: 141 LVMAQANVSRAKAVTALKENNSDVVNAIMS 170


>SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 695

 Score = 27.9 bits (59), Expect = 1.4
 Identities = 23/72 (31%), Positives = 35/72 (48%)
 Frame = +2

Query: 224 SKSKQSRGEKRPER**VNSVSNQYKV*RE*QSENQRTFSLSSTRLMYTRILIQTPTLFLV 403
           SKSK  +  KR +   +NSV +  KV ++ + +     S +   L+    LI+     LV
Sbjct: 220 SKSKSFQKHKRLDEQLLNSVKSMKKVSQQLKPQKNTNDSNNDHTLLSQDQLIELSK--LV 277

Query: 404 KPRLKICPHRPP 439
           KPR K+    PP
Sbjct: 278 KPRTKLLLLGPP 289


>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1063

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 11/26 (42%), Positives = 17/26 (65%)
 Frame = +2

Query: 38  QQYIKMPELTELDKATASMTEKRKEE 115
           ++YIK  +LTELD     +T+ RK +
Sbjct: 205 KEYIKKCQLTELDPTQQLLTQSRKNQ 230


>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
            Sen1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1687

 Score = 25.0 bits (52), Expect = 10.0
 Identities = 20/57 (35%), Positives = 28/57 (49%), Gaps = 4/57 (7%)
 Frame = +1

Query: 271  SKLGLK--PVQG--VERVTIRKSKNILFVINSPDVYKNPHSDTYIVFGEAKIEDLST 429
            +KL LK  PV+G  VER  +    +I   +   D+Y  P  DT +V      ED S+
Sbjct: 976  TKLDLKFSPVEGIMVERTAVNNFVDIGVSVAPKDLYGYPLYDTEVVSLAFNKEDASS 1032


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,345,115
Number of Sequences: 5004
Number of extensions: 41981
Number of successful extensions: 118
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 111
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 117
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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