BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30370 (676 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC25H2.05 |egd2|nac1|nascent polypeptide-associated complex al... 71 1e-13 SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces pomb... 28 1.4 SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 27 2.5 SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 25 10.0 >SPBC25H2.05 |egd2|nac1|nascent polypeptide-associated complex alpha subunit Egd2|Schizosaccharomyces pombe|chr 2|||Manual Length = 173 Score = 71.3 bits (167), Expect = 1e-13 Identities = 36/80 (45%), Positives = 54/80 (67%) Frame = +1 Query: 235 TVSW*EEARKIMSKLGLKPVQGVERVTIRKSKNILFVINSPDVYKNPHSDTYIVFGEAKI 414 TV E+A+K++ KLGLK V+G+ RV +R+ KNIL +IN P VYK+ ++ YIV G+ + Sbjct: 20 TVVHAEKAQKLIQKLGLKRVEGITRVAMRRPKNILLIINEPIVYKS-SNNAYIVLGKVTV 78 Query: 415 EDLSTQATMAAAERFKAPET 474 ED++ QA + +A ET Sbjct: 79 EDMAAQARAFNESQKQATET 98 Score = 35.1 bits (77), Expect = 0.009 Identities = 17/30 (56%), Positives = 23/30 (76%) Frame = +3 Query: 585 IVMSQANVSRAKAVRALKNNHLTLLMQLWS 674 +VM+QANVSRAKAV ALK N+ ++ + S Sbjct: 141 LVMAQANVSRAKAVTALKENNSDVVNAIMS 170 >SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces pombe|chr 1|||Manual Length = 695 Score = 27.9 bits (59), Expect = 1.4 Identities = 23/72 (31%), Positives = 35/72 (48%) Frame = +2 Query: 224 SKSKQSRGEKRPER**VNSVSNQYKV*RE*QSENQRTFSLSSTRLMYTRILIQTPTLFLV 403 SKSK + KR + +NSV + KV ++ + + S + L+ LI+ LV Sbjct: 220 SKSKSFQKHKRLDEQLLNSVKSMKKVSQQLKPQKNTNDSNNDHTLLSQDQLIELSK--LV 277 Query: 404 KPRLKICPHRPP 439 KPR K+ PP Sbjct: 278 KPRTKLLLLGPP 289 >SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1063 Score = 27.1 bits (57), Expect = 2.5 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = +2 Query: 38 QQYIKMPELTELDKATASMTEKRKEE 115 ++YIK +LTELD +T+ RK + Sbjct: 205 KEYIKKCQLTELDPTQQLLTQSRKNQ 230 >SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1687 Score = 25.0 bits (52), Expect = 10.0 Identities = 20/57 (35%), Positives = 28/57 (49%), Gaps = 4/57 (7%) Frame = +1 Query: 271 SKLGLK--PVQG--VERVTIRKSKNILFVINSPDVYKNPHSDTYIVFGEAKIEDLST 429 +KL LK PV+G VER + +I + D+Y P DT +V ED S+ Sbjct: 976 TKLDLKFSPVEGIMVERTAVNNFVDIGVSVAPKDLYGYPLYDTEVVSLAFNKEDASS 1032 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,345,115 Number of Sequences: 5004 Number of extensions: 41981 Number of successful extensions: 118 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 111 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 117 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 309878492 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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