Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30362
(614 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g30870.1 68415.m03762 glutathione S-transferase, putative sup... 40 0.001
At1g02940.1 68414.m00261 glutathione S-transferase, putative sim... 39 0.002
At5g41240.1 68418.m05011 glutathione S-transferase, putative sim... 38 0.007
At2g30860.1 68415.m03761 glutathione S-transferase, putative ide... 38 0.007
At5g41210.1 68418.m05008 glutathione S-transferase (GST10) ident... 37 0.012
At5g41220.1 68418.m05009 glutathione S-transferase, putative sim... 35 0.037
At3g62760.1 68416.m07050 glutathione S-transferase, putative Glu... 35 0.037
At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1... 34 0.086
At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1... 34 0.086
At2g02380.1 68415.m00176 glutathione S-transferase, putative sim... 33 0.15
At5g17220.1 68418.m02018 glutathione S-transferase, putative 31 0.46
At2g34990.1 68415.m04293 zinc finger (C3HC4-type RING finger) fa... 31 0.46
At3g03190.1 68416.m00315 glutathione S-transferase, putative ide... 31 0.61
At1g62660.1 68414.m07071 beta-fructosidase (BFRUCT3) / beta-fruc... 31 0.61
At1g12240.1 68414.m01416 beta-fructosidase (BFRUCT4) / beta-fruc... 31 0.61
At1g02950.2 68414.m00263 glutathione S-transferase, putative sim... 30 1.1
At1g02950.1 68414.m00262 glutathione S-transferase, putative sim... 30 1.1
At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6) iden... 30 1.4
At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1... 29 1.9
At1g05080.1 68414.m00510 F-box family protein contains F-box dom... 29 1.9
At5g27160.1 68418.m03241 hypothetical protein contains Pfam prof... 29 2.4
At2g03760.1 68415.m00336 steroid sulfotransferase, putative stro... 28 4.3
At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 28 5.7
At4g07520.1 68417.m01174 hypothetical protein contains Pfam prof... 27 7.5
At1g79400.1 68414.m09253 cation/proton exchanger, putative (CHX2... 27 7.5
At1g20690.1 68414.m02591 expressed protein 27 7.5
At5g38830.1 68418.m04697 tRNA synthetase class I (C) family prot... 27 9.9
At4g04480.1 68417.m00650 hypothetical protein 27 9.9
At3g07960.1 68416.m00973 phosphatidylinositol-4-phosphate 5-kina... 27 9.9
At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 27 9.9
>At2g30870.1 68415.m03762 glutathione S-transferase, putative
supported by cDNA GI:443698 GB:D17673
Length = 215
Score = 39.9 bits (89), Expect = 0.001
Identities = 18/34 (52%), Positives = 23/34 (67%)
Frame = +2
Query: 152 GEQLKPEYLKLNPQHTVPTLVDDGLSIWESRAII 253
GEQ +PEYL + P +P LVD I+ESRAI+
Sbjct: 37 GEQRQPEYLAIQPFGKIPVLVDGDYKIFESRAIM 70
>At1g02940.1 68414.m00261 glutathione S-transferase, putative
similar to glutathione S-transferase GI:860955 from
[Hyoscyamus muticus]
Length = 256
Score = 39.1 bits (87), Expect = 0.002
Identities = 17/33 (51%), Positives = 22/33 (66%)
Frame = +2
Query: 152 GEQLKPEYLKLNPQHTVPTLVDDGLSIWESRAI 250
G+Q KP +L +NP VP +D GL + ESRAI
Sbjct: 74 GDQKKPSFLAINPFGQVPVFLDGGLKLTESRAI 106
>At5g41240.1 68418.m05011 glutathione S-transferase, putative
similar to glutathione S-transferase, GST 10b
GB:CAA10662 [Arabidopsis thaliana] 37349.
Length = 591
Score = 37.5 bits (83), Expect = 0.007
Identities = 15/33 (45%), Positives = 23/33 (69%)
Frame = +2
Query: 155 EQLKPEYLKLNPQHTVPTLVDDGLSIWESRAII 253
+QL PE+ ++NP VP +VD L ++ES AI+
Sbjct: 39 QQLSPEFKEINPMGKVPAIVDGRLKLFESHAIL 71
>At2g30860.1 68415.m03761 glutathione S-transferase, putative
identical to GB:Y12295
Length = 215
Score = 37.5 bits (83), Expect = 0.007
Identities = 17/34 (50%), Positives = 22/34 (64%)
Frame = +2
Query: 152 GEQLKPEYLKLNPQHTVPTLVDDGLSIWESRAII 253
GE +P YL L P TVP +VD I+ESRA++
Sbjct: 37 GEHKQPAYLALQPFGTVPAVVDGDYKIFESRAVM 70
>At5g41210.1 68418.m05008 glutathione S-transferase (GST10)
identical to glutathione transferase AtGST 10
[Arabidopsis thaliana] GI:4049401
Length = 245
Score = 36.7 bits (81), Expect = 0.012
Identities = 17/44 (38%), Positives = 26/44 (59%)
Frame = +2
Query: 155 EQLKPEYLKLNPQHTVPTLVDDGLSIWESRAIITTW*TSTPKAA 286
+QL PE+ +NP VP +VD L ++ES AI+ ++ P A
Sbjct: 40 QQLSPEFKDINPLGKVPAIVDGRLKLFESHAILIYLSSAFPSVA 83
>At5g41220.1 68418.m05009 glutathione S-transferase, putative
similar to emb|CAA10662
Length = 590
Score = 35.1 bits (77), Expect = 0.037
Identities = 15/33 (45%), Positives = 21/33 (63%)
Frame = +2
Query: 155 EQLKPEYLKLNPQHTVPTLVDDGLSIWESRAII 253
+QL PE+ +NP VP +VD L + ES AI+
Sbjct: 39 QQLSPEFKDINPMGKVPAIVDGKLKLSESHAIL 71
>At3g62760.1 68416.m07050 glutathione S-transferase, putative
Glutathione transferase III(b) - Zea mays, EMBL:AJ010296
Length = 219
Score = 35.1 bits (77), Expect = 0.037
Identities = 15/28 (53%), Positives = 20/28 (71%)
Frame = +2
Query: 167 PEYLKLNPQHTVPTLVDDGLSIWESRAI 250
P +L +NP VP L DD L+++ESRAI
Sbjct: 43 PSFLSMNPFGKVPALQDDDLTLFESRAI 70
>At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1)
(GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
(Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
{Arabidopsis thaliana}; contains Pfam profiles PF02798:
Glutathione S-transferase, N-terminal domain and
PF00043:Glutathione S-transferase, C-terminal domain
Length = 191
Score = 33.9 bits (74), Expect = 0.086
Identities = 16/34 (47%), Positives = 22/34 (64%)
Frame = +2
Query: 152 GEQLKPEYLKLNPQHTVPTLVDDGLSIWESRAII 253
G+Q ++ K+NP TVP LVD + I +S AII
Sbjct: 44 GDQFDSDFKKINPMGTVPALVDGDVVINDSFAII 77
>At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1)
(GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
(Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
{Arabidopsis thaliana}; contains Pfam profiles PF02798:
Glutathione S-transferase, N-terminal domain and
PF00043:Glutathione S-transferase, C-terminal domain
Length = 221
Score = 33.9 bits (74), Expect = 0.086
Identities = 16/34 (47%), Positives = 22/34 (64%)
Frame = +2
Query: 152 GEQLKPEYLKLNPQHTVPTLVDDGLSIWESRAII 253
G+Q ++ K+NP TVP LVD + I +S AII
Sbjct: 44 GDQFDSDFKKINPMGTVPALVDGDVVINDSFAII 77
>At2g02380.1 68415.m00176 glutathione S-transferase, putative
similar to gi:167970 gb:AAA72320 gb:AY052332
Length = 223
Score = 33.1 bits (72), Expect = 0.15
Identities = 16/34 (47%), Positives = 22/34 (64%)
Frame = +2
Query: 152 GEQLKPEYLKLNPQHTVPTLVDDGLSIWESRAII 253
G+Q ++ K+NP TVP LVD + I +S AII
Sbjct: 47 GDQSDSDFKKINPMGTVPALVDGDVVINDSFAII 80
>At5g17220.1 68418.m02018 glutathione S-transferase, putative
Length = 214
Score = 31.5 bits (68), Expect = 0.46
Identities = 15/32 (46%), Positives = 19/32 (59%)
Frame = +2
Query: 155 EQLKPEYLKLNPQHTVPTLVDDGLSIWESRAI 250
EQ KPE+L P VP + D ++ESRAI
Sbjct: 39 EQKKPEHLLRQPFGQVPAIEDGDFKLFESRAI 70
>At2g34990.1 68415.m04293 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 302
Score = 31.5 bits (68), Expect = 0.46
Identities = 22/75 (29%), Positives = 34/75 (45%), Gaps = 1/75 (1%)
Frame = +1
Query: 70 LCALQGCTADGEGAQLKPEPQTGRPTPRGTAQTRIFEVEPSTHGPDPSRRWSFNLGVPRH 249
LC + C GE + L PEP T + +F+ T+ PSR WS L R
Sbjct: 136 LCRVDLCLQPGERSYLNPEPDLVEST-----NSHLFDGVTWTNRNRPSRSWSTRLSQCRV 190
Query: 250 HHYLVNK-YAKGSSL 291
L+++ ++ G S+
Sbjct: 191 SQILISRSHSTGHSV 205
>At3g03190.1 68416.m00315 glutathione S-transferase, putative
identical to glutathione S-transferase GB:AAB09584 from
[Arabidopsis thaliana]
Length = 214
Score = 31.1 bits (67), Expect = 0.61
Identities = 15/32 (46%), Positives = 20/32 (62%)
Frame = +2
Query: 155 EQLKPEYLKLNPQHTVPTLVDDGLSIWESRAI 250
EQ KP++L P VP + D L ++ESRAI
Sbjct: 39 EQKKPQHLLRQPFGQVPAIEDGYLKLFESRAI 70
>At1g62660.1 68414.m07071 beta-fructosidase (BFRUCT3) /
beta-fructofuranosidase / invertase, vacuolar identical
to beta-fructosidase GB:CAA67560 GI:1429209 [Arabidopsis
thaliana]; supported by full-length cDNA GI:14517549;
identical to cDNA Beta-fructosidase GI:3115854
Length = 648
Score = 31.1 bits (67), Expect = 0.61
Identities = 19/78 (24%), Positives = 36/78 (46%), Gaps = 1/78 (1%)
Frame = +1
Query: 160 AQTRIFEVEPSTHGPDPSRRWSFNLGVPRHHHYLVNKYAKGSSLY-PEDPKARALVDQRL 336
++T++ ++ S +GPD ++ R HY + Y ++ + P++P + L
Sbjct: 306 SKTQLNGLDTSVNGPDVKHVIKASMDDTRIDHYAIGTYDDSNATWVPDNPSIDVGISTGL 365
Query: 337 YFDIGTLYQRFSDYFYPQ 390
+D G Y S FY Q
Sbjct: 366 RYDYGKYYA--SKTFYDQ 381
>At1g12240.1 68414.m01416 beta-fructosidase (BFRUCT4) /
beta-fructofuranosidase / invertase, vacuolar identical
to beta-fructosidase GI:1871503 from [Arabidopsis
thaliana]; contains Pfam profile PF00251:Glycosyl
hydrolases family 32; identical to cDNA
beta-fructosidase (vacuolar form) GI:1321683; similar to
SP:Q43857
Length = 664
Score = 31.1 bits (67), Expect = 0.61
Identities = 20/71 (28%), Positives = 30/71 (42%), Gaps = 1/71 (1%)
Frame = +1
Query: 181 VEPSTHGPDPSRRWSFNLGVPRHHHYLVNKYAKGSSLY-PEDPKARALVDQRLYFDIGTL 357
++ S +GPD ++ R HY V Y + + P+DP + L +D G
Sbjct: 328 LDTSVNGPDVKHIVKASMDDTRFDHYAVGTYFDSNGTWIPDDPTIDVGMTASLRYDYGKF 387
Query: 358 YQRFSDYFYPQ 390
Y S FY Q
Sbjct: 388 YA--SKSFYDQ 396
>At1g02950.2 68414.m00263 glutathione S-transferase, putative
similar to glutathione-S-transferase GI:169887 from
[Silene vulgaris]
Length = 245
Score = 30.3 bits (65), Expect = 1.1
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = +2
Query: 152 GEQLKPEYLKLNPQHTVPTLVDDGLSIWESRAI 250
GE +L LNP VP D + ++ESRAI
Sbjct: 62 GEHKTEPFLSLNPFGQVPVFEDGSVKLYESRAI 94
>At1g02950.1 68414.m00262 glutathione S-transferase, putative
similar to glutathione-S-transferase GI:169887 from
[Silene vulgaris]
Length = 243
Score = 30.3 bits (65), Expect = 1.1
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = +2
Query: 152 GEQLKPEYLKLNPQHTVPTLVDDGLSIWESRAI 250
GE +L LNP VP D + ++ESRAI
Sbjct: 60 GEHKTEPFLSLNPFGQVPVFEDGSVKLYESRAI 92
>At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6)
identical to GB:X95295. Based on identical cDNA hits,
the translation is now 40 AAs longer at the N-terminal,
and start of exon2 is also corrected.
Length = 263
Score = 29.9 bits (64), Expect = 1.4
Identities = 14/33 (42%), Positives = 20/33 (60%)
Frame = +2
Query: 152 GEQLKPEYLKLNPQHTVPTLVDDGLSIWESRAI 250
G + +L LNP +P L D L+++ESRAI
Sbjct: 87 GAHKQEAHLALNPFGQIPALEDGDLTLFESRAI 119
>At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1)
(GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
(Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
{Arabidopsis thaliana}; contains Pfam profiles PF02798:
Glutathione S-transferase, N-terminal domain and
PF00043:Glutathione S-transferase, C-terminal domain
Length = 228
Score = 29.5 bits (63), Expect = 1.9
Identities = 14/28 (50%), Positives = 19/28 (67%)
Frame = +2
Query: 170 EYLKLNPQHTVPTLVDDGLSIWESRAII 253
++ K+NP TVP LVD + I +S AII
Sbjct: 57 DFKKINPMGTVPALVDGDVVINDSFAII 84
>At1g05080.1 68414.m00510 F-box family protein contains F-box domain
Pfam:PF00646
Length = 439
Score = 29.5 bits (63), Expect = 1.9
Identities = 25/77 (32%), Positives = 37/77 (48%), Gaps = 2/77 (2%)
Frame = +2
Query: 176 LKLNPQHTVPTLVDDGLSIWESRAIITTW*TSTPKAAVCTPKTPKLVLSLTNGCISTSEL 355
++L PQ PT D + W ++A+ T T K T L SL + C S SEL
Sbjct: 90 MELGPQ--CPTTDDVDIGKWVAKAVDCLVMTLTIKLLWSAGPT-SLPKSLYS-CTSLSEL 145
Query: 356 CTRDSVIISIPK--YLP 400
D +++++P YLP
Sbjct: 146 TLSDQILVNVPSSAYLP 162
>At5g27160.1 68418.m03241 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 702
Score = 29.1 bits (62), Expect = 2.4
Identities = 13/33 (39%), Positives = 22/33 (66%)
Frame = -2
Query: 253 DDGAGLPD*KTIVD*GRDRVLRVQLQIFWFELF 155
DDG G D +VD R+R+++ + +IFW ++F
Sbjct: 412 DDGIGFVD--PVVDSWRNRLIKERKRIFWKDMF 442
>At2g03760.1 68415.m00336 steroid sulfotransferase, putative strong
similarity to steroid sulfotransferases from [Brassica
napus] GI:3420008, GI:3420004, GI:3420006; contains Pfam
profile PF00685: Sulfotransferase domain
Length = 326
Score = 28.3 bits (60), Expect = 4.3
Identities = 23/69 (33%), Positives = 36/69 (52%), Gaps = 4/69 (5%)
Frame = -3
Query: 393 YLGIEIITE---SLVQSSDVEIQPLVNESTSF-GVFGVQTAAFGVLVHQVVMMARDSQIE 226
YLG E +T+ +L+ S E LV+E F G++ Q G+L+ Q A+DS I
Sbjct: 10 YLGDEDLTQETRALISSLPKEKGWLVSEIYEFQGLWHTQAILQGILICQKRFEAKDSDII 69
Query: 225 RPSSTRVGT 199
++ + GT
Sbjct: 70 LVTNPKSGT 78
>At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative similar to elongation factor 1B
gamma GI:3868758 from [Oryza sativa]
Length = 413
Score = 27.9 bits (59), Expect = 5.7
Identities = 14/28 (50%), Positives = 17/28 (60%)
Frame = +2
Query: 167 PEYLKLNPQHTVPTLVDDGLSIWESRAI 250
PE+LK+NP VP L I+ES AI
Sbjct: 43 PEFLKMNPIGKVPVLETPEGPIFESNAI 70
>At4g07520.1 68417.m01174 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 734
Score = 27.5 bits (58), Expect = 7.5
Identities = 12/33 (36%), Positives = 21/33 (63%)
Frame = -2
Query: 253 DDGAGLPD*KTIVD*GRDRVLRVQLQIFWFELF 155
DDG G D +VD R+R+++ + + FW ++F
Sbjct: 417 DDGIGFVD--PVVDSWRNRLIKERKRFFWKDMF 447
>At1g79400.1 68414.m09253 cation/proton exchanger, putative (CHX2)
monovalent cation:proton antiporter family 2 (CPA2)
member, PMID:11500563
Length = 783
Score = 27.5 bits (58), Expect = 7.5
Identities = 13/31 (41%), Positives = 20/31 (64%), Gaps = 1/31 (3%)
Frame = -1
Query: 581 NVGVFLEVNVRGFQAGDTGDETQ-VATVRFG 492
++G+F++ N+ GFQ D Q VAT+ FG
Sbjct: 588 SIGIFVDRNITGFQQPHGFDSVQHVATLFFG 618
>At1g20690.1 68414.m02591 expressed protein
Length = 240
Score = 27.5 bits (58), Expect = 7.5
Identities = 10/27 (37%), Positives = 19/27 (70%)
Frame = -3
Query: 378 IITESLVQSSDVEIQPLVNESTSFGVF 298
++ E +++SSD+E+ E+ S+GVF
Sbjct: 139 VVREKMIRSSDIELHVYDMEADSWGVF 165
>At5g38830.1 68418.m04697 tRNA synthetase class I (C) family protein
similar to SP|Q06752 Cysteinyl-tRNA synthetase (EC
6.1.1.16) (Cysteine--tRNA ligase) (CysRS) {Bacillus
subtilis}; contains Pfam profile PF01406: tRNA
synthetases class I (C)
Length = 511
Score = 27.1 bits (57), Expect = 9.9
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = -2
Query: 604 LTVSYHLLTLAYFLKSMSEASRLETLAMRLKS 509
+T SYH L L +FL S S L A +L+S
Sbjct: 285 ITASYHPLALRHFLMSAQYRSPLSFTASQLES 316
>At4g04480.1 68417.m00650 hypothetical protein
Length = 398
Score = 27.1 bits (57), Expect = 9.9
Identities = 15/34 (44%), Positives = 23/34 (67%), Gaps = 1/34 (2%)
Frame = +2
Query: 227 SIWESRAIITTW*TST-PKAAVCTPKTPKLVLSL 325
S+ SR ++++ +ST PKAAV PK PK +S+
Sbjct: 3 SLQVSRLVLSSSSSSTRPKAAVSIPKLPKFHVSV 36
>At3g07960.1 68416.m00973 phosphatidylinositol-4-phosphate 5-kinase
family protein similar to
phosphatidylinositol-4-phosphate 5-kinase AtPIP5K1
[Arabidopsis thaliana] GI:3702691; contains Pfam
profiles PF01504: Phosphatidylinositol-4-phosphate
5-Kinase, PF02493: MORN repeat
Length = 715
Score = 27.1 bits (57), Expect = 9.9
Identities = 13/38 (34%), Positives = 19/38 (50%)
Frame = +1
Query: 127 PQTGRPTPRGTAQTRIFEVEPSTHGPDPSRRWSFNLGV 240
P +G TP G ++TR+ E D S+ S LG+
Sbjct: 591 PTSGARTPTGNSETRLSRAEMDRFLLDASKLASIKLGI 628
>At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative Similar to elongation factor
1-gamma (gb|EF1G_XENLA). ESTs
gb|T20564,gb|T45940,gb|T04527 come from this gene
Length = 414
Score = 27.1 bits (57), Expect = 9.9
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +2
Query: 167 PEYLKLNPQHTVPTLVDDGLSIWESRAI 250
P +LK+NP VP L S++ES AI
Sbjct: 43 PAFLKMNPIGKVPVLETPEGSVFESNAI 70
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,914,266
Number of Sequences: 28952
Number of extensions: 273414
Number of successful extensions: 841
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 824
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 841
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1236350304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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