Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30352
(453 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g39840.1 68417.m05645 expressed protein 40 8e-04
At3g11890.2 68416.m01458 expressed protein 36 0.013
At5g67360.1 68418.m08494 cucumisin-like serine protease (ARA12) ... 33 0.068
At2g40070.1 68415.m04923 expressed protein 32 0.16
At5g01280.1 68418.m00037 expressed protein 31 0.28
At4g17410.1 68417.m02607 expressed protein 31 0.36
At3g11890.1 68416.m01457 expressed protein 31 0.36
At1g66470.1 68414.m07551 basic helix-loop-helix (bHLH) family pr... 30 0.64
At2g40270.2 68415.m04955 protein kinase family protein contains ... 29 1.9
At2g40270.1 68415.m04954 protein kinase family protein contains ... 29 1.9
At1g51355.1 68414.m05776 expressed protein 29 1.9
At5g45770.1 68418.m05627 leucine-rich repeat family protein cont... 27 7.8
At5g40340.1 68418.m04894 PWWP domain-containing protein KED, Nic... 27 7.8
At4g26630.1 68417.m03837 expressed protein 27 7.8
At2g28110.1 68415.m03415 exostosin family protein contains 1 tra... 27 7.8
At1g69550.1 68414.m07998 disease resistance protein (TIR-NBS cla... 27 7.8
At1g69330.1 68414.m07954 zinc finger (C3HC4-type RING finger) fa... 27 7.8
>At4g39840.1 68417.m05645 expressed protein
Length = 451
Score = 39.9 bits (89), Expect = 8e-04
Identities = 26/87 (29%), Positives = 45/87 (51%), Gaps = 3/87 (3%)
Frame = +1
Query: 10 SSSPSQKQTTKVLKPTTQSSSITPKSATRKVTSVASTTVGSKKLTSSTTVKPASTSKKV- 186
SSS ++K TK+LKP + SSS ++ K T++ ++ + +SS T K +S KK+
Sbjct: 86 SSSGTKKNQTKLLKPISSSSSTKNQTKLAKTTTMGTSHKLNSTKSSSNTTKTSSELKKLN 145
Query: 187 --TEKQSFTKKMTVDVFVAKKSKGAQK 261
T+ + T + ++K S K
Sbjct: 146 SGTKSTNSTSSIKKSADLSKSSSSKNK 172
>At3g11890.2 68416.m01458 expressed protein
Length = 527
Score = 35.9 bits (79), Expect = 0.013
Identities = 22/73 (30%), Positives = 34/73 (46%)
Frame = +1
Query: 49 KPTTQSSSITPKSATRKVTSVASTTVGSKKLTSSTTVKPASTSKKVTEKQSFTKKMTVDV 228
KP+T S ++ TRK T V + + S + ++ STS+ E ++ T K T V
Sbjct: 280 KPSTSQSQAELRAKTRKATLVHAAAIPSLPVPEHYSLLKPSTSQSQAEVRTLTHKAT-HV 338
Query: 229 FVAKKSKGAQKHC 267
A S +HC
Sbjct: 339 HTAMPSLSVTEHC 351
Score = 31.1 bits (67), Expect = 0.36
Identities = 17/58 (29%), Positives = 30/58 (51%)
Frame = +1
Query: 49 KPTTQSSSITPKSATRKVTSVASTTVGSKKLTSSTTVKPASTSKKVTEKQSFTKKMTV 222
KP T S ++ TRK T V + + S + +++ STS+ E ++ T+K T+
Sbjct: 242 KPATSQSQAELRANTRKATLVHAAAMPSLPVPEHYSLQKPSTSQSQAELRAKTRKATL 299
Score = 28.3 bits (60), Expect = 2.6
Identities = 16/50 (32%), Positives = 26/50 (52%)
Frame = +1
Query: 43 VLKPTTQSSSITPKSATRKVTSVASTTVGSKKLTSSTTVKPASTSKKVTE 192
+LKP+T S ++ T K T V T + S +T T++ +TS+ E
Sbjct: 316 LLKPSTSQSQAEVRTLTHKATHV-HTAMPSLSVTEHCTLQKPATSQSQAE 364
>At5g67360.1 68418.m08494 cucumisin-like serine protease (ARA12)
Asp48; almost identical to cucumisin-like serine
protease (ARA12) GI:3176874 from [Arabidopsis thaliana]
Length = 757
Score = 33.5 bits (73), Expect = 0.068
Identities = 23/51 (45%), Positives = 29/51 (56%), Gaps = 6/51 (11%)
Frame = +1
Query: 91 TRKVTSVASTTVGSKKLTSSTT-----VKPASTS-KKVTEKQSFTKKMTVD 225
TR VTSV S K+TS TT V+PA + K+ EK+S+T TVD
Sbjct: 676 TRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVD 726
>At2g40070.1 68415.m04923 expressed protein
Length = 607
Score = 32.3 bits (70), Expect = 0.16
Identities = 19/61 (31%), Positives = 31/61 (50%)
Frame = +1
Query: 7 RSSSPSQKQTTKVLKPTTQSSSITPKSATRKVTSVASTTVGSKKLTSSTTVKPASTSKKV 186
RS+ + + T + + T+ SSS +A++ TS A + + T STT K A S+
Sbjct: 226 RSTVSATTKPTPMSRSTSLSSSRLTPTASKPTTSTARSAGSVTRSTPSTTTKSAGPSRST 285
Query: 187 T 189
T
Sbjct: 286 T 286
Score = 29.1 bits (62), Expect = 1.5
Identities = 15/59 (25%), Positives = 31/59 (52%), Gaps = 1/59 (1%)
Frame = +1
Query: 7 RSSSPSQKQT-TKVLKPTTQSSSITPKSATRKVTSVASTTVGSKKLTSSTTVKPASTSK 180
R S+P+ + T + +P+ +S T + T+ ST++ S +LT + + ST++
Sbjct: 204 RPSTPTSRATVSSATRPSLTNSRSTVSATTKPTPMSRSTSLSSSRLTPTASKPTTSTAR 262
Score = 28.7 bits (61), Expect = 1.9
Identities = 22/63 (34%), Positives = 29/63 (46%)
Frame = +1
Query: 1 ARRSSSPSQKQTTKVLKPTTQSSSITPKSATRKVTSVASTTVGSKKLTSSTTVKPASTSK 180
+R +S S + T KPTT T +SA S STT S + STT ST++
Sbjct: 239 SRSTSLSSSRLTPTASKPTTS----TARSAGSVTRSTPSTTTKSAGPSRSTTPLSRSTAR 294
Query: 181 KVT 189
T
Sbjct: 295 SST 297
>At5g01280.1 68418.m00037 expressed protein
Length = 460
Score = 31.5 bits (68), Expect = 0.28
Identities = 25/74 (33%), Positives = 35/74 (47%), Gaps = 1/74 (1%)
Frame = +1
Query: 4 RRSSSPSQKQTTKVLKPTTQSSSITPKSATRKVTSVASTTVGSKKLT-SSTTVKPASTSK 180
RR SS S ++T PT S TP TS A++T LT SSTT S S+
Sbjct: 83 RRPSSSSSSRSTS-RPPTPTRKSKTPAKRPSTPTSRATSTTTRATLTSSSTTSSTRSWSR 141
Query: 181 KVTEKQSFTKKMTV 222
+ + T ++T+
Sbjct: 142 PSSSSGTGTSRVTL 155
Score = 31.1 bits (67), Expect = 0.36
Identities = 21/72 (29%), Positives = 38/72 (52%), Gaps = 2/72 (2%)
Frame = +1
Query: 1 ARRSSSPSQKQTTKVLKPTTQSSSITPKSATRKVTSVASTTVGSK-KLTSSTTVKP-AST 174
A+R S+P+ + T+ + T SSS T + + S +S T S+ LT++ +P ST
Sbjct: 108 AKRPSTPTSRATSTTTRATLTSSSTTSSTRSWSRPSSSSGTGTSRVTLTAARATRPTTST 167
Query: 175 SKKVTEKQSFTK 210
++ T + T+
Sbjct: 168 DQQTTGSATSTR 179
>At4g17410.1 68417.m02607 expressed protein
Length = 744
Score = 31.1 bits (67), Expect = 0.36
Identities = 16/62 (25%), Positives = 29/62 (46%)
Frame = +1
Query: 7 RSSSPSQKQTTKVLKPTTQSSSITPKSATRKVTSVASTTVGSKKLTSSTTVKPASTSKKV 186
+ +PS T LKP+ + + IT A+ ++ V S + S+ K A+ S+
Sbjct: 310 KKPAPSNNNETSTLKPSIEIAEITSAWASAEIVKVEKPVDASANIQGSSNGKEAAVSQLN 369
Query: 187 TE 192
T+
Sbjct: 370 TQ 371
>At3g11890.1 68416.m01457 expressed protein
Length = 500
Score = 31.1 bits (67), Expect = 0.36
Identities = 17/58 (29%), Positives = 30/58 (51%)
Frame = +1
Query: 49 KPTTQSSSITPKSATRKVTSVASTTVGSKKLTSSTTVKPASTSKKVTEKQSFTKKMTV 222
KP T S ++ TRK T V + + S + +++ STS+ E ++ T+K T+
Sbjct: 242 KPATSQSQAELRANTRKATLVHAAAMPSLPVPEHYSLQKPSTSQSQAELRAKTRKATL 299
Score = 27.9 bits (59), Expect = 3.4
Identities = 15/49 (30%), Positives = 24/49 (48%)
Frame = +1
Query: 49 KPTTQSSSITPKSATRKVTSVASTTVGSKKLTSSTTVKPASTSKKVTEK 195
KP+T S ++ TRK T V + + S + ++ STS+ EK
Sbjct: 280 KPSTSQSQAELRAKTRKATLVHAAAIPSLPVPEHYSLLKPSTSQSQAEK 328
>At1g66470.1 68414.m07551 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain
Length = 298
Score = 30.3 bits (65), Expect = 0.64
Identities = 20/59 (33%), Positives = 31/59 (52%), Gaps = 2/59 (3%)
Frame = +1
Query: 43 VLKPTTQSSSITPKSATRKVTSVASTTVGSKKLTSSTT--VKPASTSKKVTEKQSFTKK 213
V+ +++ S++PKSA K + +T SKKL+S T KP T+ + QS K
Sbjct: 154 VVTTGSRNESLSPKSAGNKRSHTGESTQPSKKLSSGVTGKTKPKPTTSP-KDPQSLAAK 211
>At2g40270.2 68415.m04955 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 482
Score = 28.7 bits (61), Expect = 1.9
Identities = 15/53 (28%), Positives = 29/53 (54%)
Frame = +1
Query: 19 PSQKQTTKVLKPTTQSSSITPKSATRKVTSVASTTVGSKKLTSSTTVKPASTS 177
PS+K + T + S +P+ A + V++ AST +K +S+ V P+ ++
Sbjct: 63 PSRKDRKNRVVAATTTPSSSPEPAPKHVSTKASTVSEPQKRSSTQDVSPSPSA 115
>At2g40270.1 68415.m04954 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 489
Score = 28.7 bits (61), Expect = 1.9
Identities = 15/53 (28%), Positives = 29/53 (54%)
Frame = +1
Query: 19 PSQKQTTKVLKPTTQSSSITPKSATRKVTSVASTTVGSKKLTSSTTVKPASTS 177
PS+K + T + S +P+ A + V++ AST +K +S+ V P+ ++
Sbjct: 70 PSRKDRKNRVVAATTTPSSSPEPAPKHVSTKASTVSEPQKRSSTQDVSPSPSA 122
>At1g51355.1 68414.m05776 expressed protein
Length = 116
Score = 28.7 bits (61), Expect = 1.9
Identities = 28/92 (30%), Positives = 39/92 (42%), Gaps = 1/92 (1%)
Frame = +1
Query: 1 ARRSSSPSQKQTTKVLKPTTQSSSITPKSATRKVTSVASTTVGSKKLTSSTTVKPAST-S 177
A + P ++ TT+ K + + P S VTS +ST S TS+ T P S S
Sbjct: 2 ASKGKKPLRRTTTRRRKRSHFKNPSPPCSINSDVTSTSST---STSPTSTATPSPVSAES 58
Query: 178 KKVTEKQSFTKKMTVDVFVAKKSKGAQKHCXR 273
T ++S +M KK K AQ R
Sbjct: 59 GCCTPEKSRIPEMLTCPPAPKKQKVAQNCALR 90
>At5g45770.1 68418.m05627 leucine-rich repeat family protein
contains leucine rich-repeat domains Pfam:PF00560,
INTERPRO:IPR001611
Length = 425
Score = 26.6 bits (56), Expect = 7.8
Identities = 17/42 (40%), Positives = 23/42 (54%), Gaps = 2/42 (4%)
Frame = +1
Query: 58 TQSSSITPKSAT--RKVTSVASTTVGSKKLTSSTTVKPASTS 177
+ SSSIT A+ R +TS++ T S S T+KP S S
Sbjct: 56 SSSSSITCDDASPYRHITSISFTNCSSTLSLPSKTLKPLSKS 97
>At5g40340.1 68418.m04894 PWWP domain-containing protein KED,
Nicotiana tabacum, EMBL:AB009883
Length = 1008
Score = 26.6 bits (56), Expect = 7.8
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +3
Query: 210 EDDGGRFCCEEIERSSKTLXTSGII 284
ED G + C EIE + KTL G++
Sbjct: 11 EDAGPKPCSAEIESAEKTLKDDGVV 35
>At4g26630.1 68417.m03837 expressed protein
Length = 763
Score = 26.6 bits (56), Expect = 7.8
Identities = 22/69 (31%), Positives = 31/69 (44%)
Frame = +1
Query: 4 RRSSSPSQKQTTKVLKPTTQSSSITPKSATRKVTSVASTTVGSKKLTSSTTVKPASTSKK 183
+ S +K+T K PT SSS + +SA + S +T V K L S + K+
Sbjct: 472 KSSKGAKRKRTPKKTSPTAGSSS-SKRSAKSQKKSEEATKVVKKSLAHSD--DESEEEKE 528
Query: 184 VTEKQSFTK 210
EKQ K
Sbjct: 529 EEEKQEEEK 537
Score = 26.6 bits (56), Expect = 7.8
Identities = 18/70 (25%), Positives = 33/70 (47%), Gaps = 3/70 (4%)
Frame = +1
Query: 1 ARRSSSP---SQKQTTKVLKPTTQSSSITPKSATRKVTSVASTTVGSKKLTSSTTVKPAS 171
A +SS P +QK+++ K T S +PK+++++ S S++ KP
Sbjct: 609 AAKSSPPEKITQKRSSAKRKKTDDDSDTSPKASSKRKKSENPIKASPAPSKSASKEKPVK 668
Query: 172 TSKKVTEKQS 201
+ K +K S
Sbjct: 669 RAGKGKDKPS 678
>At2g28110.1 68415.m03415 exostosin family protein contains 1
transmembrane domain; similar to
pectin-glucuronyltransferase (GI:23821292) [Nicotiana
plumbaginifolia]; similar to NpGUT1 homolog
(GI:23821294) [Arabidopsis thaliana]; contains Pfam
profile PF03016: Exostosin family
Length = 448
Score = 26.6 bits (56), Expect = 7.8
Identities = 20/67 (29%), Positives = 27/67 (40%), Gaps = 5/67 (7%)
Frame = -1
Query: 342 NVAKPPRIRPTQIHKTTHQLLC---QTXAVFLSSFRFLRNKNVHRHLLGKRLFFS--NLF 178
NV PP I P +HKT + + VF L KN+ KR+ + +
Sbjct: 246 NVVIPPYISPESLHKTQKNIPVTKERDIWVFFRGKMELHPKNISGRFYSKRVRTNIWRSY 305
Query: 177 AGTSRFY 157
G RFY
Sbjct: 306 GGDRRFY 312
>At1g69550.1 68414.m07998 disease resistance protein (TIR-NBS
class), putative domain signature TIR-NBS exists,
suggestive of a disease resistance protein.
Length = 703
Score = 26.6 bits (56), Expect = 7.8
Identities = 16/38 (42%), Positives = 20/38 (52%)
Frame = +1
Query: 64 SSSITPKSATRKVTSVASTTVGSKKLTSSTTVKPASTS 177
SSS +P S+ TS ST S SS+T P+S S
Sbjct: 38 SSSTSPPSSLASSTSPPSTLSSSTSHPSSSTSPPSSLS 75
>At1g69330.1 68414.m07954 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 270
Score = 26.6 bits (56), Expect = 7.8
Identities = 16/55 (29%), Positives = 27/55 (49%)
Frame = -2
Query: 293 HTNYYARRXQCF*APFDFFATKTSTVIFLVKDCFSVTFLLVLAGFTVVLDVNFLL 129
H+N +R+ F DFF + TS F++ F + + G ++L + FLL
Sbjct: 189 HSNQPSRQLFSFHKSLDFFISFTSKFPFVI--IFLLIVFFAIPGSLIILALYFLL 241
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,651,032
Number of Sequences: 28952
Number of extensions: 188477
Number of successful extensions: 721
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 693
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 717
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 742437000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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